*Target (protein/gene name): beta-lactamase class A

*NCBI Gene # or RefSeq#: 1280 [NCBI] & Refseq #: KSA61805.1
*Protein ID (NP or XP #) or Wolbachia#: NP_878023.1
*Organism (including strain): staphylococcus aureus PC1
Etiologic Risk Group (see link below):
*/Disease Information (sort of like the Intro to your Mini Research Write up): Staphylococcus aureus is a gram-positive bacteria grouped with Bacillus sp based on ribosomal RNA sequences. This coccus bacterium grows in both aerobic and anaerobic conditions, forming grape-like clusters. Its resides in mainly the nasal membranes and skin of warm-blooded animals, causing infections ranging from mild, such as skin infections and food poisoning, to life-threatening, such as pneumonia, sepsis, osteomyelitis, and infectious endocarditis. A major concern is the MRSA infection (meticillin-resistant S aureus) which shows up as a skin infection, prominent in medical facilities. This bacteria is highly resistant to antibiotics including methicillin and the potent β-lactam antibiotics.Beta-lactamase is the enzyme involved in bacterial resistance to beta-lactam antibiotics, and if it is inhibited, then these antibiotics could work.


Link to TDR Targets page (if present): http://vdsstream.wikispaces.com/Targets ?

Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) -
1280 [NCBI]
https://www.patricbrc.org/portal/portal/patric/Feature?cType=feature&cId=RefSeq.46170.176.LFUU01000013.CDS.6906.7472.fwd

Essentiality of this protein:
Is it a monomer or multimer as biological unit? (make prediction at http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): Appears to be monomeric
Complex of proteins?: In class 2a of beta-lactamases - most common. Not in a complex with other proteins
Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):
It is druggable – similar to PBP4 target and has some known drugs already.

http://database.idrb.cqu.edu.cn/TTD/ZFTTDDetail.asp?ID=TTDS00228 - Lists drugs already discovered


*EC#: 3.5.2.6
Link to BRENDA EC# page:
http://www.brenda-enzymes.info/enzyme.php?ecno=3.5.2.6
Specifically:
http://www.brenda-enzymes.info/enzyme.php?ecno=3.5.2.6&Suchword=&organism%5B%5D=Staphylococcus+aureus+PC1&show_tm=0
-- Show screenshot of BRENDA enzyme mechanism schematic - No schematic given

Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):
-- link to Sigma (or other company) page for assay (see Sigma links below)
-- -or link (or citation) to paper that contains assay information
-- links to assay reagents (substrates) pages.
--- List cost and quantity of substrate reagents, supplier, and catalog #

Structure (PDB or Homology model)
-- PDB # or closest PDB entry if using homology model:
-- For Homology Model option:
---- Show pairwise alignment of your BLASTP search in NCBI against the PDB
---- Query Coverage:
---- Max % Identities:
---- % Positives
---- Chain used for homology:

Current Inhibitors:
Expression Information (has it been expressed in bacterial cells):
Purification Method:
Image of protein (PyMol with features delineated and shown separately):
*Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):
*length of your protein in Amino Acids
Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website
Molar Extinction coefficient of your protein at 280 nm wavelength:
TMpred graph Image (http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
*CDS Gene Sequence (paste as text only):
*GC% Content for gene:
*CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):
*GC% Content for gene (codon optimized):

Do Not Need this info for Spring (but still copy these lines to your Target page for now)
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):
(link to DNA Works output text file - that should be saved in your Google Docs folder after you did the primer design protocol)
-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.

Primer design results for 'tail' primers (this is just 2 sequences):
**