Protein+phosphatase+2C+(Toxoplasma+Gondii)

//Toxoplasma gondii// Targets a Protein Phosphatase 2C to the Nuclei of Infected Host Cells Research Paper: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1800361/

Protein Link from Paper: http://www.ncbi.nlm.nih.gov/protein/?Db=protein&DbFrom=pmc&Cmd=Link&LinkName=pmc_protein&IdsFromResult=1800361

Link to Protein Phosphatase 2C: http://www.ncbi.nlm.nih.gov/protein/ABO31335.1


 * Target (protein/gene name): Protein Phosphatase 2C **
 * *NCBI Gene # or RefSeq#: ** ABO31335.1 GI:129716418
 * *Protein ID (NP or XP #) or Wolbachia#: **


 * *Organism (including strain): **Toxoplasma Gondii


 * Etiologic Risk Group (see link below): **


 * *Background/Disease Information (sort of like the Intro to your Mini Research Write up): ** Toxoplasma Gondii is a eukaryotic parasitic protozoan that causes toxoplasmosis. This organism is found worldwide and capable of infecting all warm-blooded animals. As one of the most common human parasites, T. Gondii has been predicted to have infected up to a third of the global population. Although the parasite is found worldwide, the disease itself (toxoplasmosis) only causes mild flu-like symptoms. However in infants and HIV/AIDS patients, this infection cause serious and potentially fatal illness. Infection of this disease usually occurs due to consumption of raw/undercooked meat, ingesting soil/vegetables/water contaminated with cat feces, or through transmission from mother to fetus during pregnancy if mother is infected.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1800361/
 * Essentiality of this protein: ** Essential
 * Complex of proteins?: **
 * Druggable Target: **
 * *EC#: 3.1.3.16 **
 * Link to BRENDA EC# page: ** []
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **
 * -- List cost and quantity of substrate reagents and supplier **
 * Structure Available (PDB): ** []
 * Current Inhibitors: **
 * Expression Information (has it been expressed in bacterial cells): **
 * Purification Method: **
 * Image of protein (PyMol with features delineated and shown separately): **

ORIGINAL: maslrvlalaclplfavlhadaktllgkvkratgmgvgegpsvakkpkytatvpgftpps gdqrmnefmavdtsgefmrhlyieegrtvcasatsrnrrptsesphsddvvvvegmlrgr petrvhamfdgfqgrhsamwlaqnvmnylndlrdvneeeitrqfermdgdlraanlpggs saliifvryekkptearvvgrqivpegakeftsvaealggplmpvvamnfrrdpraakgi ytihvaslgnsrcvlksgrtaihlstphtasshkerhrvqaaggvfttvngelllggvvp mtrafgsfdfkkggqgklqqdlvsavpdvttffaypgddivagtagafahfrshaaiaaa ialypvspetvldaakamvvnakrrkvtknistfvrhlpesrtrsqkmlegtsgengeed fsidrtneltqalqagffsfllyyp
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **

"CUT DOWN" SEQUENCE (FROM RESEARCH PAPER): madaktllgkvkratgmgvgegpsvakkpkytatvpgftpps gdqrmnefmavdtsgefmrhlyieegrtvcasatsrnrrptsesphsddvvvvegmlrgr petrvhamfdgfqgrhsamwlaqnvmnylndlrdvneeeitrqfermdgdlraanlpggs saliifvryekkptearvvgrqivpegakeftsvaealggplmpvvamnfrrdpraakgi ytihvaslgnsrcvlksgrtaihlstphtasshkerhrvqaaggvfttvngelllggvvp mtrafgsfdfkkggqgklqqdlvsavpdvttffaypgddivagtagafahfrshaaiaaa ialypvspetvldaakamvvnakrrkvtknistfvrhlpesrtrsqkmlegtsgenge

Cut Sequence: 400
 * *length of your protein in Amino Acids- ** ORIGINAL: ** 445 **


 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam]website: ** 43297.3 Da
 * Molar Extinction coefficient of your protein at 280 nm wavelength: **
 * TMpred graph Image **




 * *CDS Gene Sequence (paste as text only): **

ATGGCGGATGCTAAAACC CTGCTCGGTAAAGTTAAACGTGCGACCGGTATGGGTGTTGGTGAAGGTCCGTCTGT TGCGAAAAAACCGAAATACACCGCGACCGTTCCTGGCTTTACTCCGCCGTCTGGTGACCAGCGTATGAACGAGT TCATGGCAGTTGACACCTCTGGCGAATTCATGCGCCACCTGTACATCGAGGAAGGTCGTACCGTTTGCGCGTCT GCGACTTCTCGTAATCGTCGCCCGACCTCTGAATCTCCGCACTCTGACGATGTTGTTGTGGTTGAGGGTATGCT GCGTGGTCGTCCGGAAACCCGTGTTCACGCGATGTTCGACGGTTTCCAGGGTCGTCACTCTGCGATGTGGCTGG CGCAGAACGTTATGAACTACCTGAACGACCTGCGCGACGTCAACGAGGAGGAAATTACCCGTCAGTTTGAACGC ATGGACGGTGATCTCCGTGCAGCGAACCTGCCGGGTGGCTCTAGCGCCCTGATCATCTTTGTTCGCTACGAAAA GAAACCAACCGAAGCGCGTGTAGTTGGCCGTCAGATCGTCCCGGAGGGTGCGAAGGAATTCACTTCTGTGGCGG AAGCGCTGGGCGGTCCACTGATGCCTGTCGTTGCGATGAACTTCCGTCGTGACCCGCGTGCTGCGAAAGGTATC TACACCATCCACGTAGCGTCTCTGGGTAACTCTCGTTGTGTACTGAAATCTGGCCGTACCGCGATCCACCTCTC TACCCCGCACACTGCCTCTTCTCACAAAGAACGCCACCGCGTACAAGCCGCTGGCGGTGTCTTTACGACGGTAA ATGGTGAACTGCTGCTGGGTGGTGTTGTCCCGATGACCCGTGCGTTCGGTTCTTTCGACTTCAAAAAAGGCGGT CAGGGTAAACTGCAGCAGGACCTCGTTTCTGCGGTTCCTGATGTTACCACCTTCTTCGCGTACCCAGGCGATGA CATCGTTGCAGGTACTGCGGGTGCTTTCGCGCACTTTCGTTCTCATGCCGCAATCGCTGCGGCTATTGCCCTGT ATCCGGTTTCTCCTGAGACTGTTCTGGACGCCGCAAAGGCTATGGTTGTTAACGCGAAACGTCGCAAAGTTACC AAAAACATCTCTACGTTCGTTCGTCACCTCCCGGAATCTCGTACCCGTTCCCAGAAAATGCTGGAAGGTACTTC CGGTGAAAACGGCGAATAA


 * *GC% Content for gene: 55.36% **


 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **

TACTTCCAATCC ATGGCGGATGCTAAAACC CTGCTCGGTAAAGTTAAACGTGCGACCGGTATGGGTGTTGGTGAAGGTCCGTC TGTTGCGAAAAAACCGAAATACACCGCGACCGTTCCTGGCTTTACTCCGCCGTCTGGTGACCAGCGTATGAACGAGTTCATGG CAGTTGACACCTCTGGCGAATTCATGCGCCACCTGTACATCGAGGAAGGTCGTACCGTTTGCGCGTCTGCGACTTCTCGTAAT CGTCGCCCGACCTCTGAATCTCCGCACTCTGACGATGTTGTTGTGGTTGAGGGTATGCTGCGTGGTCGTCCGGAAACCCGTGT TCACGCGATGTTCGACGGTTTCCAGGGTCGTCACTCTGCGATGTGGCTGGCGCAGAACGTTATGAACTACCTGAACGACCTGC GCGACGTCAACGAGGAGGAAATTACCCGTCAGTTTGAACGCATGGACGGTGATCTCCGTGCAGCGAACCTGCCGGGTGGCTCT AGCGCCCTGATCATCTTTGTTCGCTACGAAAAGAAACCAACCGAAGCGCGTGTAGTTGGCCGTCAGATCGTCCCGGAGGGTGC GAAGGAATTCACTTCTGTGGCGGAAGCGCTGGGCGGTCCACTGATGCCTGTCGTTGCGATGAACTTCCGTCGTGACCCGCGTG CTGCGAAAGGTATCTACACCATCCACGTAGCGTCTCTGGGTAACTCTCGTTGTGTACTGAAATCTGGCCGTACCGCGATCCAC CTCTCTACCCCGCACACTGCCTCTTCTCACAAAGAACGCCACCGCGTACAAGCCGCTGGCGGTGTCTTTACGACGGTAAATGG TGAACTGCTGCTGGGTGGTGTTGTCCCGATGACCCGTGCGTTCGGTTCTTTCGACTTCAAAAAAGGCGGTCAGGGTAAACTGC AGCAGGACCTCGTTTCTGCGGTTCCTGATGTTACCACCTTCTTCGCGTACCCAGGCGATGACATCGTTGCAGGTACTGCGGGT GCTTTCGCGCACTTTCGTTCTCATGCCGCAATCGCTGCGGCTATTGCCCTGTATCCGGTTTCTCCTGAGACTGTTCTGGACGC CGCAAAGGCTATGGTTGTTAACGCGAAACGTCGCAAAGTTACCAAAAACATCTCTACGTTCGTTCGTCACCTCCCGGAATCTC GTACCCGTTCCCAGAAAATGCTGGAAGGTACTTCCGGTGAAAACGGCGAATAACAGTAAAGGTGGATA


 * *GC% Content for gene (codon optimized): 55.04% **

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences): **

Forward: TACTTCCAATCC ATGGCGGATGCTAAAACC

Reverse: TATCCACCTTTACTGTTATTCGCCGTTTTCACC


 * The oligonucleotide primer sequences: **

1 ATGGCGGATGCTAAAACCCTGCTCGGTAAAGTTAAACGTGCG 42 2 TTTCGCAACAGACGGACCTTCACCAACACCCATACCGGTCGCACGTTTAACTTTACCGAG 60 3 GGTCCGTCTGTTGCGAAAAAACCGAAATACACCGCGACCGTTCCTGGCTTTACTCCGCCG 60 4 AGGTGTCAACTGCCATGAACTCGTTCATACGCTGGTCACCAGACGGCGGAGTAAAGCCAG 60 5 GTTCATGGCAGTTGACACCTCTGGCGAATTCATGCGCCACCTGTACATCGAGGAAGGTCG 60 6 CGGGCGACGATTACGAGAAGTCGCAGACGCGCAAACGGTACGACCTTCCTCGATGTACAG 60 7 TCTCGTAATCGTCGCCCGACCTCTGAATCTCCGCACTCTGACGATGTTGTTGTGGTTGAG 60 8 CGCGTGAACACGGGTTTCCGGACGACCACGCAGCATACCCTCAACCACAACAACATCGTC 60 9 AAACCCGTGTTCACGCGATGTTCGACGGTTTCCAGGGTCGTCACTCTGCGATGTGGCTGG 60 10 TTGACGTCGCGCAGGTCGTTCAGGTAGTTCATAACGTTCTGCGCCAGCCACATCGCAGAG 60 11 ACCTGCGCGACGTCAACGAGGAGGAAATTACCCGTCAGTTTGAACGCATGGACGGTGATC 60 12 TGATCAGGGCGCTAGAGCCACCCGGCAGGTTCGCTGCACGGAGATCACCGTCCATGCGTT 60 13 GCTCTAGCGCCCTGATCATCTTTGTTCGCTACGAAAAGAAACCAACCGAAGCGCGTGTAG 60 14 AAGTGAATTCCTTCGCACCCTCCGGGACGATCTGACGGCCAACTACACGCGCTTCGGTTG 60 15 GGGTGCGAAGGAATTCACTTCTGTGGCGGAAGCGCTGGGCGGTCCACTGATGCCTGTCGT 60 16 ATACCTTTCGCAGCACGCGGGTCACGACGGAAGTTCATCGCAACGACAGGCATCAGTGGA 60 17 GCGTGCTGCGAAAGGTATCTACACCATCCACGTAGCGTCTCTGGGTAACTCTCGTTGTGT 60 18 CGGGGTAGAGAGGTGGATCGCGGTACGGCCAGATTTCAGTACACAACGAGAGTTACCCAG 60 19 GATCCACCTCTCTACCCCGCACACTGCCTCTTCTCACAAAGAACGCCACCGCGTACAAGC 60 20 CCAGCAGCAGTTCACCATTTACCGTCGTAAAGACACCGCCAGCGGCTTGTACGCGGTGGC 60 21 AATGGTGAACTGCTGCTGGGTGGTGTTGTCCCGATGACCCGTGCGTTCGGTTCTTTCGAC 60 22 ACGAGGTCCTGCTGCAGTTTACCCTGACCGCCTTTTTTGAAGTCGAAAGAACCGAACGCA 60 23 CTGCAGCAGGACCTCGTTTCTGCGGTTCCTGATGTTACCACCTTCTTCGCGTACCCAGGC 60 24 GAAAGTGCGCGAAAGCACCCGCAGTACCTGCAACGATGTCATCGCCTGGGTACGCGAAGA 60 25 GTGCTTTCGCGCACTTTCGTTCTCATGCCGCAATCGCTGCGGCTATTGCCCTGTATCCGG 60 26 AACCATAGCCTTTGCGGCGTCCAGAACAGTCTCAGGAGAAACCGGATACAGGGCAATAGC 60 27 GCCGCAAAGGCTATGGTTGTTAACGCGAAACGTCGCAAAGTTACCAAAAACATCTCTACG 60 28 GAACGGGTACGAGATTCCGGGAGGTGACGAACGAACGTAGAGATGTTTTTGGTAACTTTG 60 29 CGGAATCTCGTACCCGTTCCCAGAAAATGCTGGAAGGTACTTCCGGTGAAAACGGCGAAT 60 30 TTATTCGCCGTTTTCACCGG 20