Target-+YopH+(S.+aureus)-------------------------------------Stephanie+Bsaibes

A sudden onset of fever, chills, vomiting, diarrhea, muscle aches and rash characterize Toxic Shock Syndrome and can progress into severe hypotension. The bacterium colonizes skin and mucous membranes in humans. [for Yersinia= gastrointestinal syndromes to Bubonic Plague] []
 * *Target (protein/gene name): **YopH (protein-tyrosine-phosphatase)
 * *NCBI Gene # or RefSeq#: **
 * *Protein ID (NP or XP #) or Wolbachia#: ** NP_783699
 * *Organism: **//Staphylococcus aureus/ Yersinia entercolitica //
 * Etiologic Risk Group (see link below): **“usually exotoxin producing strains of //Staphylococcus aureus//”
 * *Background/Disease Information: **[]
 * Essentiality of this protein: **[]
 * Complex of enzymes: **no
 * *EC#: **3.1.3.48
 * Link to BRENDA EC# page: **[]
 * <span style="font-family: 'Calibri','sans-serif';">Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **

-- []
 * <span style="font-family: 'Calibri','sans-serif';">-- **[] (assay proof?)

-[]
 * <span style="font-family: 'Calibri','sans-serif';">Structure Available (PDB or Homology model) **
 * <span style="font-family: 'Calibri','sans-serif';">-- PDB # or closest PDB entry if using homology model: **<span style="font-family: 'Calibri','sans-serif';">1K46
 * <span style="font-family: 'Calibri','sans-serif';">-- Query Coverage (if not direct match): **
 * <span style="font-family: 'Calibri','sans-serif';">-- Max Ident (if not direct match): **
 * <span style="font-family: 'Calibri','sans-serif';">Druggable Target (see Databases for this): **<span style="font-family: 'Calibri','sans-serif';">Yes
 * <span style="font-family: 'Calibri','sans-serif';">Current Inhibitors: **[]
 * <span style="font-family: 'Calibri','sans-serif';">Expression Information (has it been expressed in bacterial cells): --- ****<span style="font-family: 'Calibri','sans-serif';">[] **


 * <span style="font-family: 'Calibri','sans-serif';">Purification Method: ****<span style="font-family: 'Calibri','sans-serif';">http://onlinelibrary.wiley.com/doi/10.1046/j.1462-5822.2000.00061.x/full#ss2 **
 * <span style="font-family: 'Calibri','sans-serif';">Image of protein (PyMol or etc): **

>gi|28373018|ref|NP_783699.1| YopH [Yersinia enterocolitica] MNLSLSDLHRQVSRLVQQESGDCTGKLRGNVAANKETTFQGLTIASGARESEKVFAQTVLSHVANIVLTQ EDTAKLLQSTVKHNLNNYELRSVGNGNSVLVSLRSDQMTLQDAKVLLEAALRQESGARGHVSSHSHSVLH APGTPVREGLRSHLDPRTPPLPPRERPHTSGHHGAGEARATAPSTVSPYGPEARAELSSRLTTLRNTLTP ATNDPRYLQACGGEKLNRFRDIQCCRQTAVRADLNANYIQVGNTRTIACQYPLQSQLESHFRMLAENRTP VLAVLASSSEIANQRFGMPDYFRQSGTYGSITVESKMTQQVGLGDGIMADMYTLTIREAGQKTISVPVVH VGNWPDQTAVSSEVTKALASLVDQTAETKRNMYESKGSSAVADDSKLRPVIHCRAGVGRTAQLIGAMCMN DNRNSQLSVEDMVSQMRVQRNGIMVQKDEQLDVLIKLAEGQGRPLLNS
 * [[image:steph.jpg width="400" height="400"]] ||
 * Image of YopH ||  ||
 * <span style="font-family: 'Calibri','sans-serif';">*Amino Acid Sequence: **

<span style="font-family: 'Calibri','sans-serif'; font-size: 14.6667px;"> **<span style="font-family: 'Calibri','sans-serif';">*length of your protein in Amino Acids: **<span style="font-family: 'Calibri','sans-serif';">468 <span style="font-family: 'Calibri','sans-serif'; font-size: 14.6667px;"> **<span style="font-family: 'Calibri','sans-serif';">Molecular Weight of your protein in kiloDaltons using the **[|**Expasy ProtParam**]**<span style="font-family: 'Calibri','sans-serif';"> website: **50996.4 ATGAACTTATCATTAAGCGATCTTCATCGTCAGGTATCTCGATTGGTGCAGCAAGAGAGCGGTGATT GTA CCGGGAAATTAAGAGGTAACGTTGCTGCCAATAAAGAAACTACCTTTCAAGGTTTGACCATAG CCAGTGGTGCCAGAGAGTCAGAAAAAGTATTTGCTCAAACTGTACTAAGCCACGTAGCAAATATTG TTCTAACTCAAGAAGATACCGCTAAGCTATTGCAAAGCACGGTAAAGCATAATTTGAATAATTATGAA TTAAGAAGTGTCGGCAATGGTAATAGTGTACTTGTCAGTTTACGTAGTGACCAAATGACACTACAAG ACGCCAAAGTGCTGTTGGAGGCTGCATTGCGACAAGAGTCGGGAGCGAGGGGGCATGTATCATC TCATTCACATTCAGTCCTTCACGCACCGGGAACTCCGGTGCGTGAAGGACTGCGTTCACATCTAGA CCCCAGAACACCACCGTTGCCACCGCGTGAACGACCACACACTTCTGGCCATCACGGGGCTGGC GAAGCCAGAGCCACCGCACCAAGCACTGTTTCTCCTTATGGCCCAGAAGCGCGCGCAGAACTCA GCAGCCGCCTCACCACATTGCGCAATACGCTGACGCCAGCAACGAATGATCCGCGTTACTTACAAG CCTGCGGCGGTGAAAAGCTAAACCGATTTAGAGATATTCAATGCTGTCGGCAAACCGCAGTACGCG CCGATCTTAATGCCAATTACATCCAGGTCGGTAACACTCGTACCATAGCGTGCCAGTATCCGCTACAA TCTCAACTTGAAAGCCATTTCCGTATGCTGGCAGAAAACCGAACGCCAGTGTTGGCTGTTTTAGCGT CCAGTTCTGAGATAGCCAATCAAAGATTCGGTATGCCAGATTATTTCCGCCAGAGTGGTACCTATGGC AGTATCACTGTAGAGTCTAAAATGACTCAGCAAGTTGGTCTCGGTGACGGGATTATGGCAGATATGTA TACTTTAACGATTCGTGAAGCGGGTCAAAAAACAATTTCTGTTCCTGTGGTTCATGTTGGCAATTGGCC CGATCAGACCGCAGTCAGCTCTGAAGTTACCAAGGCACTCGCTTCACTGGTAGATCAAACAGCAGA AACAAAACGCAATATGTATGAAAGCAAAGGAAGTTCAGCGGTAGCAGATGACTCCAAATTACGGCC GGTAATACATTGCCGTGCGGGTGTTGGCCGTACTGCGCAACTGATTGGCGCAATGTGCATGAATGAT AATCGTAATAGTCAGTTAAGCGTAGAAGATATGGTCAGCCAAATGCGAGTACAAAGAAATGGTATTAT GGTACAAAAAGATGAGCAACTTGATGTTCTGATTAAGTTGGCTGAAGGACAAGGGCGACCATTATTA AATAGCTAA
 * <span style="font-family: 'Calibri','sans-serif'; font-size: 14.6667px;">Molar Extinction coefficient of your protein at 280 nm wavelength: **<span style="font-family: 'Calibri','sans-serif'; font-size: 14.6667px;">19285
 * <span style="font-family: 'Calibri','sans-serif';">*CDS Gene Sequence: **


 * <span style="font-family: 'Calibri','sans-serif';">*GC% Content for gene: ****<span style="font-family: 'Calibri','sans-serif';">46.9% **
 * <span style="font-family: 'Calibri','sans-serif';">*CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol: **
 * <span style="font-family: 'Calibri','sans-serif';">*GC% Content for gene (codon optimized): **