MentorsFall15

= **MENTOR TASKS** = NOTE: the best way to figure out what you need to work on is to do 'Ctrl+F' and search for your name. Hit 'enter' to find the next instance. However, some tasks say ' ** All ** ' so your name won't show up specifically - Thanks, Dr. B - this assumes I haven't misspelled your name! Mentors: **Andee, Charina, Nikki, Cidia, Brianna** Research Gurus: **Avery, Alberto, Luis** Lab Tech: **John G.**

Lab Safety mentors - Please fill out your weekly schedule:- ** ALL ** Use the MentorLabLog on GDocs/VDSstaff for a Lab Notebook for Wet Lab tasks - ** ALL ** Put new dates on Daily Schedule (i.e. take last year's items for each week and put the new dates for this year there) - Send out FRI Facebook link - Send out FRI LinkedIn link -

Lab Notebook Grading
Cidia is the point person for all notebook grading Lab Notebook A - due by Students on Saturday Sept 6th - divide up notebooks among mentors ---Cidia, Brianna, Nikki --> Springers ---Andee, Charina --> Veterans - comment and assign preliminary grades using the rubric for Fall - have them close to finished by Tuesday morning - All meet with Dr. B at 11 am in lab on Tuesday Sept 8th to review and approve grades - Finalize and return to cubbies by Tuesday night - Cidia enters grades to Canvas

Create a Lab Sign in Sheet for the folder at front of lab. Use last semester's as a guide. See GDrive - Brianna - DONE

Journal Club
Set up journal club page for Fall 2015 - make schedule of presenters **-**

Research Presentations Schedule -
- Mentors to update -

Lab Safety (Monday)
LAB SAFETY MENTORS: Update Lab Safety page in Wikispaces with names of each students and table, link from left hand menu.

Print Pink & Blue & Orange Sign Off Sheet for Site Specific training (Autoclave, Centrifuge) in GDocs/Misc/ --- SignOffSheet_SiteSpecific_Centrifuge_Autoclave_etc_Blue.docx - --- SignOffSheet_SiteSpecific_Centrifuge_Autoclave_etc_Pink.docx - --- SignOffSheet_SiteSpecific_Centrifuge_Autoclave_etc_Pink.docx -
 * See One, Do One, Teach One **

- designate Summer Plasmids for students to Transform and make more of -
 * Summer Plasmids **

Update Hours spreadsheet in GDocs - Tally weekly hours from sign in sheet and enter into spreadsheet on VDSStaff -
 * Hours for Students **

Create ** Research Pages ** for each student (OR SHOULD WE JUST DO DROPBOX FOLDERS? - no Diana's google sheets embed is pretty good) - (use Research Pages from Summer as a guide) - Help Springers set up their research pages according to how we did it this summer - Ask Diane Chau. Go through all of the Veterans and Springers pages and make sure they do this - you can just add this text to their page to let them know:

"Put a line and 'Week 1&2', 'Week 3&4', etc.


 * put a horizontal line after each section so you can tell where each begins or ends"

- make Target Selection form (copy from Summ15 - or make fresh) - once we know which targets are options
 * Targets **

PCR, RE Digest, Cloning, etc. - ?
 * Teaching Modules ** (in class brief activities – 5 slides, 1 activity, 2 slides)

=MENTOR TASKS=

TASKS FOR 1st WEEK:
Take a new set of pictures of the mentors with their names - to put on the this Mentors page. Take groups of 2 or 3 or use individuals. - schedule mentor meeting - ? - or have Mentors attend students' mini-meetings? prepare mentor schedule on GDocs - Andee - clean up the extra pages (keep template1, spacer page and 'blank version') prepare student schedule on GDocs - Nikki - DONE make a copy of 'hours' chart in GDocs and Archive under new name (Summer) - clear existing Hours chart and add/substract name - SHEET CREATED - just needs names of students use VDS Mendeley account and upload journal papers - update VDS google Website and upload this past years posters as PDFs (have Dr. B add you or VDSstaff@gmail as an editor)-

Walk through TDRTargets Exercise - on computer -

ADMINISTRATIVE/OFFICE WORK
view and revise our lab 'mission statement' - **ALL** update mentor schedules - post Labnotebook Grading rubric to Wiki (upload PDF then in space.menu on lhs - make link to file) -

Create new 'blank' pages on the Research Pages for each new student. - Organize them into Veterans, Springers Copy the '2 Weeks Layout' from teh Timeline F15 to the Research Pages page:

Create a Tips&Hints section on the Journal Club page for reading articles– i.e. read the Conclusion first, etc., -

Set up a Springers schedule for this week to do Nanodrop & Submit to DNA Sequencing - - use Spring schedules as a template - it will be a spreadsheet that we have open time on - then they will sign up for time slots (each time has 6-8 openings(

In 'Pigeon Holes' for lab notebooks
organize pigeon holes by moving VDS Alums to the other lab notebook stacks - organize binders (Student one and Mentor one) -


 * Mentor Folder:**
 * 1) print new timesheet pay periods sheet (from UT webpage - Timesheet Pay Periods and Due Dates (see bottom of link)
 * 2) create and print new hourly sign-in sheet (don't need to do TA though) **- see old one in GDocs either VDSStaff or /Mentors**
 * Student Folder:**
 * 1) put new sign in sheet for students
 * 2) make picture roster of students and staff pics (get photo roster from Dr. B - but add in staff pictures)

**Check Ice Room access list -**

LAB WORK
general lab cleaning - **ALL**

Grow overnight culture of pGBR22 (4x80 ml) - Midiprep 2 of them the next day - save the other two in freezer as pellets for backup - make LB -

Check that we have EcoRI and PvuII enzymes Check that we have enough pGBR22 for DNA sequencing on Thursday/Friday

Prep collirollers - split into 2 bottles and rinse then autoclave, then distribute ~6 beads/tube into 600 ul microcentrifuge tubes in a sterile fashion and store in 96well plastic 'brick' in our cubby. - make a full ~ 80 tube rack of these

1 person make a 100 bp ladder (need to have received it from ordering) - clean pH probe and bottle and replace solution -

Make some Amp plates (~20) Make some Kan plates (~20)

Make autoclaved water in 2x100 ml volumes (store at room temp). Then aliquot several into 1.7 ml centrifuge tubes, label and store in -20degC. -

put specs in correct boxes, see if we are missing anything - Get rid of old LB media and wash bottles - organize red Sigma Kit boxes (PCR Cleanup, Gel Extraction, Miniprep Kit). Consolidate and then discard all but two boxes for each type: current box and a spare solutions box. - Organize plates by Staff, vs. Summer, vs etc. - -- Put contaminated plates in zip locs, put a note on them that they are for disposal, but leave in fridge in a certain spot for the user to dispose of (I want them to see first).

Update Laptops and Desktops -

 * Start up laptops and do Windows and Browser updates on them. Remove security credentials. -
 * Clean off old icons from Desktops of __Desktop__ & __Laptop__ computers-
 * Install both printers on each laptop and each Desktop (see below on this page for instructions) -

Lab Tech Tasks (John G) - see Purchasing sheet on GDrive for items
@https://drive.google.com/open?id=19gXV3i-WeguSKluGbEH21akQqgE--S4UhNEUKmzalx4&authuser=0

Open, figure out if it is VDS, show to John G. if there, Label appropriately, Place in proper storage, Place receipt in Dr. B's pigeon hole by the lab notebooks and let him know it has arrived and is stored properly.
 * What do I do with a package that is delivered to lab?: **

**SOLUTIONS RECIPES & INSTRUCTIONS:**

 * (also see the Solutions&Dilutions Spreadsheet on GDocs for help with calculations) **


 * Make LB ** - 2Liters - and autoclave
 * Make 5 M NaCl ** - 500 ml in water, sterile filter into a bottle, label, store at Room Temp


 * Make Kanamycin ** - stocks 50ug/ul store in -20degC, Make 30 tubes of 500 ul each. __ Sterile filter with syringe filter __ before aliquoting into tubes. Label. Store in -20degC


 * Make IPTG ** - 1 M IPTG stock to make. Make 30 tubes of this with 500 ul in each. Label. Store in -20degC


 * Make Lysozyme ** - 20 aliquots of 500 ul at 50 mg/ml in 1.7 ml tubes. Label. Store in -20degC


 * Make TCEP - ** make ~50 x 100ul aliquots of 500 mM aliquots medium size centrifuge tubes. Label, put in a box and Store at Room Temp in cubby.


 * Make PMSF - ** 30 x 500 ul aliquots of 10mM in ** __Isop____ropanol__ ** 1.7 ml tubes. Label. store in -20degC


 * Make Imidazole ** - 1M stock ** make in beaker of 500 ml of water, then bottle top sterile filter it into a bottle, store at Room Temp **

=== Lysis Buffer for Sonication (make 500 ml) === Tris 100 mM NaCl 300 mM  Imidazole 30 mM  pH 8.0 w/ NaOH label, store at 4degC
 * (see GDocs ' ** Solutions&Dilutions ** ' in / ** Misc ** folder for calculations) **

Wash Buffer Tris 100 mM NaCl 300 mM  Imidazole 30 mM  pH 8.0 w/ NaOH
 * NEW Wash and Elution Buffers and Storage Buffer(see GDocs ' ** Solutions&Dilutions ** ' in / ** Misc ** folder for calculations) **

Tris 100 mM NaCl 300 mM  Imidazole 300 mM  pH 8.0 w/ NaOH
 * Elution Buffer **

Tris 50 mM NaCl 150 mM  pH 8.0 w/ NaOH Then filter sterilize Then degass**
 * FPLC column buffer - tris Also 'storage' buffer **

6X SDS Page Buffer from 5 Prime Protocol
30 ml of glycerol – add first so that you can see the volume (viscous) (In a separate tube) - 30 ml of Nanopure water --- 1.635 g Tris powder --- 3 g SDS poweder --- 0.3 g Bromophenol blue 2.3136 g DTT Mix contents of second tube into tube with glycerol Add some more Nanopure water to 50 ml total final volume

= Printer Installation  =
 * 1) Login as Mentor, Start Menu,
 * 2) Devices And printers
 * 3) Add New Printer
 * 4) Add "local" printer
 * 5) Create New Port --> standard TCP pathway
 * 6) Enter IP 128.82.195.186
 * 7) Printer Name Lexmark C543
 * 8) Add Printer (if can't find printer do Window Update)
 * For installation of a different Printer (such as Dell) change the IP address and Printer Name **

Printer Name: Lexmark C543 IP: 128.83.195.186 Printer Name: Dell IP: 129.116.152.49

= COMPLETED STUFF++++++++++++++++++++++++++++++++++++++++++++++++++++++++ =