Target+-+deoxyuridine+triphosphatase+(Leishmania+major)

dUTP works together with thymidylate synthase in the synthesis of dTMP. When the function of this enzyme is altered the concentration of dUMP is too high and excessive DNA repair cycles occur. It ultimately results in the death of the disease causing cells.
 * *Target (protein/gene name): **deoxyuridine triphosphatase
 * *NCBI Gene # or RefSeq#: **29483
 * *Protein ID (NP or XP #) or Wolbachia#: ** LmjF06.0560
 * *Organism (including strain): **Leishmania major
 * Etiologic Risk Group (see link below): **
 * * Disease Information : **
 * Link to TDR Targets page (if present): **[|TDR information]
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.): **[|TriTrypDB]
 * Essentiality of this protein: **[|The Crystal Structure of the Leishmania major dUTP]
 * Complex of proteins: **
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **Check the article where Essensiality is dicussed.


 * *EC#: **3.6.1.23
 * Link to BRENDA EC# page: **[|BRENDA Info]




 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **
 * -- link to Sigma (or other company ) page for assay (see Sigma links below) **
 * -[|Spectrophotometric Assayability] **
 * -- links to assay reagents (substrates) pages. **
 * --- List cost and quantity of substrate reagents, supplier, and catalog # **

[|2CJE]
 * Structure (PDB or Homology model) **

>2CJE:A|PDBID|CHAIN|SEQUENCE MKRARSANIPGAILHSLAELQDGLNAMIDPSWRAVRSLDNWALAITMESTELLDSYPWKWWKNLNATPDLANVRIELVDI FHFSLSGAMQMRSTPDDEIPAASLKPLKEVMTTFLPAKECTSDPYGFVFFPLTDTQNAIASFRNIIQLANAYRFDVIIEC IIYAAEDLGFNLVAYYIAKHTLNCIRQLSGYKDGSYVKVNNGVEDNSLLHNCIKDVSLDEVLDADKYVQAWNSIMANVYE AFQIKESDRKDAERWFALAKENRLAIKA
 * Current Inhibitors: **alpha-beta-imido-dUTP, dUDP, dUMP.
 * Expression Information (has it been expressed in bacterial cells): ** Expressed in E. coli
 * Purification Method : **No commentary on purification methods.
 * Image of protein (PyMol with features delineated and shown separately): **
 * *Amino Acid Sequence **** : **


 * *length of your protein in Amino Acids: ** 268 Residues
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website: ** 30353.6
 * Molar Extinction coefficient of your protein at 280 nm wavelength: ** 53650
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
 * *CDS Gene Sequence : ** No similar human structures
 * *GC% Content for gene: **
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **
 * *GC% Content for gene (codon optimized): **

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)

**
 * Primer design results for 'tail' primers (this is just 2 sequences): **

Resources:
See ** ProtocolTargetDiscoveryVDS.docx ** for more

Etiologic Risk Group Categories (for pathogens): http://www.utexas.edu/research/rsc/ibc/agent_class.html#_Toc7238334


 * SIGMA-ALDRICH RESOURCES **

Enzyme Explorer

@http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer.html

Enzyme Classification Index (EC number)

@http://www.sigmaaldrich.com/life-science/biochemicals/biochemical-products.html?TablePage=14573088

WolframAlpha @http://www.wolframalpha.com/

DrugBank @http://www.drugbank.ca/


 * Databases of genes/organisms: **

@http://www.niaid.nih.gov/Pages/default.aspx

@http://eupathdb.org/eupathdb/

@https://patricbrc.vbi.vt.edu/portal/portal/patric/Home

@http://www.nmpdr.org/FIG/wiki/view.cgi/Main/EssentialGenes

@http://tubic.tju.edu.cn/deg/

@http://csgid.org/csgid/cake/pages/community_request_gateway

@http://tdrtargets.org/

@http://gsc.jcvi.org/status.shtml

@http://wwwnc.cdc.gov/eid/pages/scientific-nomenclature.htm
 * Scientific Nomenclature page from Center for Disease Control (gene, protein names and abbreviations) **

NCBI GENE Page: @http://www.ncbi.nlm.nih.gov/gene BLAST Page: @http://blast.ncbi.nlm.nih.gov/
 * Gene Information: **

NCBI Protein Page: @http://www.ncbi.nlm.nih.gov/protein Protein Expression Website Protein Expression Paper:
 * Protein Information: **


 * Primer Overlap PCR Articles **


 * Is my target good for Virtual Screening programs? **