Summer14

What are we doing today?
see the Daily Schedule

Schedules
please enter your summer class schedule here (if you are also taking classes in the summer or have other conflicts with lab work): LINK HERE

Mentor Schedules
Want to know when the mentors will be on? - check here Google Calendar Mentor Conflict sheet


 * Start Date**: Thursday June 5th ---> **End Date**: ~ August 1st

Group Meeting/Journal Club
once a week Location: GEA 403 (4th floor) LINK TO JOURNAL CLUB Group Meeting Slides are in the Google Drive folder

Spaces
PAI 2.14 wet lab GEA - classroom PAI 3.04H Dr. B's office WEL 2.128 computer lab Painter 1st floor - ice room, autoclave, big centrifuge Biotech lab - WEL 3.270 Robertus lab - WEL 5th floor Chemistry Storeroom - WEL 1st floor (basement) - supplies and dry ice Chemistry Electronics Shop - WEL 2nd floor (near computer lab) BioScience Storeroom - NHB 1.502 - supplies ICMB Core Facilities:
 * NHB glassware shop - next to BioSci storeroo
 * DNA Sequencing Facility (DASF) - MBB 1.426
 * ICMB Storeroom - MBB 1.426K (separate room for turning in invoice and for picking up items)
 * Can only pick up items from 9-12 AM and 2-5 PM

The Plan
We are going to work on our targets that we started picking in the Target Discovery Exercise. First, we will review those and see if they are good. Order overlap primers for our targets (we need these before we can start cloning for 'real') While we wait on the primers, we will express, purify and characterize YopH & FtHap enzymes - 2 current VDS targets. -- may also do NDM-1 and RpFabG

Then we will do some practice cloning procedures to teach you guys the new DNA cloning skills. Then we will proceed to work on cloning our targets Then we will express, purify and characterize our targets. Then we will perform enzyme activity assays to see if our targets work Then we will carry out inhibition assays

Lab Safety:
Have you complete your lab safety trainings?

Tours and Fun Stuff

 * **Naturally Obsessed Form** - when to watch this documentary- Please fill out this form....
 * == TACC Tour at VisLab in ACES building __ON CAMPUS__ - @==
 * (meet in lab before at to walk over)
 * Fill out - **Medical Waiver**
 * Fill out - **Liability form**
 * ==TACC Machine room Tour - @==
 * (meet in lab before - then we'll catch bus up to Pickle campus)
 * == GASF (Genome Sequencing and Analysis Facility) Tour in MBB 4.102 (not done) ==
 * Paper to read before the tour: NGS_Mardis.pdf
 * == Algae Lab Tour : Meet in PAI 2.14 - then will walk over to BIO 320 (not done)==

Skills List:
Target Discovery PCR (primer overlap) Restriction Enzyme Digest Cloning Plasmid Midi-Prep DNA Sequencing Protein Expression, Purification, Characterization (Large scale) FPLC Enzyme assays

Things to Keep in Mind
Wikispaces no like it when you have more than 3 windows open at a time! It be slow. Store reagents at the proper temperature & in the right location (e.g. correct freezer box) Label all reagents (tubes, boxes, liguids, powders) with VDS and Date (also include your initials if it is yours) Write down everything that you do in lab into your lab notebook Clean your area when done and return reagents and materials to their 'homes'

Summer Researchers:
Grace T, Melissa H., Nicole W., Tony G. Jesus De La O
 * Mentors:**
 * Super Researcher:**
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