Sam+U.

Welcome to my research page! The wait is over, the results of my labwork are finally here.

__ Transformation Efficiency Results __ I only got colonies to grow on one of the three plates. And here is the plate.



MidiPrep Attempt #1 The outcome of my midiprep was a tad strange. My guess is that I could have done a better job with keeping my sample pure...



Midiprep Attempt #2 This time my Midiprep results were a success. I borrowed **four** pellets of PNIC-BSa4 to ensure a high concentration of pNIC in the end.Other than that, I followed the same procedure I did the previous attempt.

My __PCR results__ have been extremely consistent. It's too bad they never turn out right. The following are my various attempts at PCR.

__Restriction Digest__ done in cahoots with Janice

Lane 1:Skipped Lane 2: 1KB ladder Lane 3: Uncut pGBR22 Lane 4: EcoRI- Janice Lane 5: Pvull Lane 6: EcoRI+Pvull Lane 7: Uncut pGBR22 Lane 8: EcoRI- Sam Lane 9: Pvull Lane 10: EcoRI+Pvull

My first successful PCR! The results from __Secondary PCR__.



After Secondary PCR, I proceeded to PCR squared. From there I did PCR cleanup, and the results were pretty bad. It wasn't that surprising seeing how I had no idea what I was doing, and I messed up on every step, including flipping the column upside down.

__PCR Cleanup__

I gave PCR clean up another try. Luckily, I had saved my Secondary PCR tube. I had put loading dye into the tube, and discovered afterward that is discouraged. The loading dye didn't make a negative impact on the results. They were a lot better.