Target-+Eonyl-(acyl+carrier+protein)+reductase+(Burkholderia+Pseudomallei+1719b)

//Burkholderia pseudomallei// is classified as a category B bioterrorism pathogen by the US National Institute of Allergy and Infectious Diseases.This organism causes disease meliodosis, often refered to as Glanders, which is found in South-East Asia and parts of Northern Australia. Few cases of this disease have been reported each year, but the lack of research and medical facilities in the countries of incidence may be the cause for the underestimate in the records.Currently, there is no vaccine to prevent melioidosis. The mortality rate is very high. Treatment using first-line agents have ended in continual relapse.
 * *Target (protein/gene name): Eonyl -(acyl carrier protein) reductase **
 * *NCBI Gene # or RefSeq#:208435803 (3EK_D) **
 * *Protein ID (NP or XP #) or Wolbachia#: **
 * *Organism (including strain): Burkholderia Pseudomallei 1719b **
 * Etiologic Risk Group (see link below):Risk II **
 * *Background/Disease Information (sort of like the Intro to your Mini Research Write up): **

Fatty acid biosynthesis (FAS) is used to synthesize the metabolic precursors for membrane phospholipids in the cell wall. Due to the essential role that fatty acids play in bacterial cell survival and the low degree of sequence homology with the mammalian FAS-I synthase, the FAS-II pathway is thought to be an attractive antibacterial drug target. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3037155/?report=reader
 * Essentiality of this protein: **
 * Complex of proteins?: Fatty Acid Synthesis II (FAS II) **
 * Druggable Target: **

[|**http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.3.1.9**]
 * *EC#: 1.3.1.9 **
 * Link to BRENDA EC# page: **


 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): Spectrophotometric **

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3037155/?report=reader#!po=15.0000
 * Assay Information: **
 * -- List cost and quantity of substrate reagents and supplier **
 * Structure Available (PDB or Homology model) **

Crystal structure of eonyl-(acyl carrier protein) reductase from burkholderia pseudomallei 1719b PDB #3EK2 

-Ceftazidime -Imipenem http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3037155/?report=reader#!po=15.0000 N-terminal His-tag on bpmFabI-2 was cleaved by treatment with thrombin.The purity of each protein was verified by 12% SDS–PAGE
 * Current Inhibitors: **
 * Expression Information: **
 * It has been expressed in E.Coli BL21(DE3) **
 * Purification Method: **

MAHHHHHHMGFLDGKRILLTGLLSNRSIAYGIAKACKREGAELAFTYVGDRFKDRITEFAAEFGSELVFPCDVADDAQID ALFASLKTHWDSLDGLVHSIGFAPREAIAGDFLDGLTRENFRIAHDISAYSFPALAKAALPMLSDDASLLTLSYLGAERA IPNYNTMGLAKAALEASVRYLAVSLGAKGVRVNAISAGPIKTLAASGIKSFGKILDFVESNSPLKRNVTIEQVGNAGAFL LSDLASGVTAEVMHVDSGFNAVVGGMAGLEE
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **

http://web.expasy.org/cgi-bin/protparam/protparam
 * *length of your protein in Amino Acids: 271 **
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website: 28795.8 **
 * Molar Extinction coefficient of your protein at 280 nm wavelength: 14565 **


 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.


 * *CDS Gene Seque **** nce (paste as text only): **
 * *GC% Content for gene: **
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **
 * *GC% Content for gene (codon optimized): **