Target+-+serine-threonine+protein+phosphatase+2C

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 * Target (protein/gene name): **serine/threonine protein phosphatase
 * *NCBI Gene # or RefSeq#: ** GI:237839921
 * *Protein ID (NP or XP #) or Wolbachia#: ** XP_002369258.1
 * *Organism (including strain): **Toxoplasma Gondii
 * Etiologic Risk Group (see link below): ** Risk Group 2 (RG2) - Parasitic Agents
 * *Background/Disease Information (sort of like the Intro to your Mini Research Write up): ** Toxoplasma Gondii is a eukaryotic parasitic protozoan that causes toxoplasmosis. This organism is found worldwide and capable of infecting all warm-blooded animals. As one of the most common human parasites, T. Gondii has been predicted to have infected up to a third of the global population. Although the parasite is found worldwide, the disease itself (toxoplasmosis) only causes mild flu-like symptoms. However in infants and HIV/AIDS patients, this infection cause serious and potentially fatal illness. Infection of this disease usually occurs due to consumption of raw/undercooked meat, ingesting soil/vegetables/water contaminated with cat feces, or through transmission from mother to fetus during pregnancy if mother is infected.
 * Essentiality of this protein: **Essential
 * Complex of proteins?: ** None
 * Druggable Target: ** No known targets, 0.3 Druggability index

[] No BRENDA information was found specifically to Toxoplasma Gondii. [] Kinase and Phosphatase Assay - Reagent:Oxadaic acid, EDTA, cyclosporin, FK-506. []
 * *EC#: ** 3.1.3.16
 * Link to BRENDA EC# page: **
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **

$68.30 - Okadaic Acid (from Sigma) [] $19.10- EDTA (from Sigma) []
 * -- List cost and quantity of substrate reagents and supplier **

-- PDB #: 2I44
 * Structure Available (PDB) **

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 * Current Inhibitors: ** EDTA, Okadaic Acid, Cyclosporin, FK-506
 * Expression Information (has it been expressed in bacterial cells): ** E. Coli
 * Purification Method: ** Immunoaffinity purification


 * Image of protein (PyMol with features delineated and shown separately): **

1 magiegpvag egaggaalgs cegpgenatk aassmslldr qieqlrrcec iperevkalc 61 akakeilvee snvqrvdvpv ticgdihgqf ydllelfrvg nflpetsylf lgdfvdrgyf 121 svetflflla lkvrypdrit lirgnhesrq itqvygfyde clrkygsstv wryctevfdy 181 lalaaiiddr lfalhgglsp aissvdeirs idrkqevphe gpmcdllwsd pedmqgwgys 241 prgagylfga divkafchtn nieiiarahq lvmdgykwwf gkklvtvwsa pnycyrcgnv 301 atvmeldeql nyqfktfeaa pperrgipsk kpppdyfl
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **
 * *length of your protein in Amino Acids- ** 338 amino acids

Ext. coefficient 57465, assuming all pairs of Cys residues form cystines Ext. coefficient 56840, assuming all Cys residues are reduced
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam]website: ** 38032.3g
 * Molar Extinction coefficient of your protein at 280 nm wavelength: **
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.



atggcgggcattgaaggcccggtggcgggcgaaggcgcgggcggcgcggcgctgggcagc tgcgaaggcccgggcgaaaacgcgaccaaagcggcgagcagcatgagcctgctggatcgc cagattgaacagctgcgccgctgcgaatgcattccggaacgcgaagtgaaagcgctgtgc gcgaaagcgaaagaaattctggtggaagaaagcaacgtgcagcgcgtggatgtgccggtg accatttgcggcgatattcatggccagttttatgatctgctggaactgtttcgcgtgggc aactttctgccggaaaccagctatctgtttctgggcgattttgtggatcgcggctatttt agcgtggaaacctttctgtttctgctggcgctgaaagtgcgctatccggatcgcattacc ctgattcgcggcaaccatgaaagccgccagattacccaggtgtatggcttttatgatgaa tgcctgcgcaaatatggcagcagcaccgtgtggcgctattgcaccgaagtgtttgattat ctggcgctggcggcgattattgatgatcgcctgtttgcgctgcatggcggcctgagcccg gcgattagcagcgtggatgaaattcgcagcattgatcgcaaacaggaagtgccgcatgaa ggcccgatgtgcgatctgctgtggagcgatccggaagatatgcagggctggggctatagc ccgcgcggcgcgggctatctgtttggcgcggatattgtgaaagcgttttgccataccaac aacattgaaattattgcgcgcgcgcatcagctggtgatggatggctataaatggtggttt ggcaaaaaactggtgaccgtgtggagcgcgccgaactattgctatcgctgcggcaacgtg gcgaccgtgatggaactggatgaacagctgaactatcagtttaaaacctttgaagcggcg ccgccggaacgccgcggcattccgagcaaaaaaccgccgccggattattttctg
 * *CDS Gene Sequence (paste as text only): **


 * *GC% Content for gene: ** 56.35%
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **
 * *GC% Content for gene (codon optimized): **

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences): **