Target+-+Protein-Tyrosine+Phosphatase+Yersinia+pestis+YopH+(Bubonic+Plague)

http://www.medicalnewstoday.com/articles/233648.php
 * Breaking News.**


 * Organism: Yersinia pestis YopH (Bubonic Plague)**
 * Target:** Protein-Tyrosine Phosphatase

What strain of //Yersinia// is this in and what is the NCBI gene #? - Dr .B


 * NCBI Gene # or RefSeq#:**
 * EC#: 3.1.3.48**

2Y2F Link -> []
 * PDB # or closest PDB entry is using homology model:**

During the 14th century, a disease known as the bubonic plague wiped out about one-third of the population in Europe. Classified as a zoonotic disease, it spread to the human population by small rodents and their fleas. Also known as the Black Death, the disease killed an infected individual within 4 days if left untreated. People with the disease experienced swollen lymph nodes (buboes), chills, high fever, muscle cramps, seizures, and change in skin color. The swollen lymph nodes were commonly found in the armpits, groin or neck, and were associated with pain. The cause of the bubonic plague was recently confirmed to the organism Yersinia pestis. It is a gram-negative bacteria that can take three main forms, the bubonic plague being the most prominent. //Yersinia pestis// attacks humans by using organisms like a flea as a vector. The bacteria accumulates inside the gut of a flea, and once the flea bites a human host, infected blood from the flea enters a human. The bacteria quickly spreads to the lymph nodes, infecting the lymphatic system. Phagocytes are not effective against //Yersinia pestis// because the bacteria can resist phagocytosis and kill them instead. As the disease progresses, lymph nodes can become necrotic and problems with the lungs can occur. Over 200 different rodents and species can also serve as hosts even though the flea is the most common. Although the disease is not extremely present in humans as it once was 7 centuries ago, cases of the plague are still reported to the World Health Organization.
 * Background/Disease:**


 * Image of protein (PyMol or etc):**




 * CDS Gene Sequence: 921 base pairs**

1 ATGCGTGAACGTCCTCACACCAGCGGTCACCACGGTGCGGGTGAGGCGCGTGCGACGGCT 61 CCTTCTACCGTTTCTCCATACGGTCCGGAAGCCCGTGCGGAACTGAGCTCTCGTCTGACC 121 ACCCTGCGTAATACCCTGGCTCCGGCGACCAACGACCCGCGTTACCTCCAGGCGTGCGGT 181 GGTGAAAAACTGAACCGTTTTCGTGACATCCAGTGTCGTCGCCAGACTGCTGTACGCGCG 241 GACCTGAACGCGAACTATATCCAGGTCGGTAACACCCGTACCATCGCGTGCCAGTATCCG 301 CTGCAATCTCAACTGGAGTCTCACTTCCGTATGCTGGCCGAAAACCGCACCCCGGTGCTC 361 GCGGTTCTGGCGTCCTCCTCTGAAATCGCTAACCAGCGCTTTGGTATGCCGGACTACTTT 421 CGTCAATCTGGTACCTACGGCTCTATCACCGTTGAATCTAAGATGACCCAGCAAGTTGGC 481 CTGGGTGACGGTATCATGGCGGACATGTACACGCTGACCATCCGTGAAGCCGGTCAGAAA 541 ACCATCAGCGTCCCAGTGGTCCACGTTGGTAACTGGCCGGATCAGACCGCAGTTAGCAGC 601 GAGGTTACGAAAGCCCTCGCGTCTCTGGTTGACCAAACGGCGGAGACTAAACGTAACATG 661 TATGAAAGCAAAGGTTCTTCTGCGGTTGCGGACGACTCTAAACTGCGTCCTGTTATTCAC 721 TGCCGTGCCGGTGTAGGTCGTACCGCGCAGCTGATCGGTGCGATGTGCATGAACGATTCT 781 CGTAACAGCCAGCTGTCTGTCGAAGATATGGTTTCTCAGATGCGTGTTCAGCGTAATGGC 841 ATCATGGTTCAGAAAGACGAACAACTCGACGTACTCATCAAACTGGCGGAAGGTCAGGGT <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 11pt; text-decoration: none; vertical-align: baseline;">901 CGTCCACTGCTGAACTCTTAA

OLIGOS (actual ones ordered from IDT DNA in 2011 - for Juan Carlos Salazar) - Dr. B Jan 12th, 2018 YopH_1 ATGCGTGAACGTCCTCACACCAGCGGTCACCACGGTGCGGGTGAGGCGCGTGCGACG YopH_2 GTTCCGCACGGGCTTCCGGACCGTATGGAGAAACGGTAGAAGGAGCCGTCGCACGCGCCT YopH_3 GAAGCCCGTGCGGAACTGAGCTCTCGTCTGACCACCCTGCGTAATACCCTGGCTCCGGCG YopH_4 TCAGTTTTTCACCACCGCACGCCTGGAGGTAACGCGGGTCGTTGGTCGCCGGAGCCAGGG YopH_5 TGCGGTGGTGAAAAACTGAACCGTTTTCGTGACATCCAGTGTCGTCGCCAGACTGCTGTA YopH_6 GGTGTTACCGACCTGGATATAGTTCGCGTTCAGGTCCGCGCGTACAGCAGTCTGGCGACG YopH_7 CTATATCCAGGTCGGTAACACCCGTACCATCGCGTGCCAGTATCCGCTGCAATCTCAACT YopH_8 CCGGGGTGCGGTTTTCGGCCAGCATACGGAAGTGAGACTCCAGTTGAGATTGCAGCGGAT YopH_9 GAAAACCGCACCCCGGTGCTCGCGGTTCTGGCGTCCTCCTCTGAAATCGCTAACCAGCGC YopH_10 CCGTAGGTACCAGATTGACGAAAGTAGTCCGGCATACCAAAGCGCTGGTTAGCGATTTCA YopH_11 CGTCAATCTGGTACCTACGGCTCTATCACCGTTGAATCTAAGATGACCCAGCAAGTTGGC YopH_12 GATGGTCAGCGTGTACATGTCCGCCATGATACCGTCACCCAGGCCAACTTGCTGGGTCAT YopH_13 ACATGTACACGCTGACCATCCGTGAAGCCGGTCAGAAAACCATCAGCGTCCCAGTGGTCC YopH_14 AACCTCGCTGCTAACTGCGGTCTGATCCGGCCAGTTACCAACGTGGACCACTGGGACGCT YopH_15 GCAGTTAGCAGCGAGGTTACGAAAGCCCTCGCGTCTCTGGTTGACCAAACGGCGGAGACT YopH_16 CCGCAACCGCAGAAGAACCTTTGCTTTCATACATGTTACGTTTAGTCTCCGCCGTTTGGT YopH_17 TTCTTCTGCGGTTGCGGACGACTCTAAACTGCGTCCTGTTATTCACTGCCGTGCCGGTGT YopH_18 GAATCGTTCATGCACATCGCACCGATCAGCTGCGCGGTACGACCTACACCGGCACGGCAG YopH_19 GCGATGTGCATGAACGATTCTCGTAACAGCCAGCTGTCTGTCGAAGATATGGTTTCTCAG YopH_20 CTTTCTGAACCATGATGCCATTACGCTGAACACGCATCTGAGAAACCATATCTTCGACAG YopH_21 ATGGCATCATGGTTCAGAAAGACGAACAACTCGACGTACTCATCAAACTGGCGGAAGGTC YopH_22 TTAAGAGTTCAGCAGTGGACGACCCTGACCTTCCGCCAGTTTGAT


 * Amino Acid Sequence:**

<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 11pt; text-decoration: none; vertical-align: baseline;">1 MRERPHTSGHHGAGEARATAPSTVSPYGPEARAELSSRLTTLRNTLAPATNDPRYLQACG <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 11pt; text-decoration: none; vertical-align: baseline;">61 GEKLNRFRDIQCRRQTAVRADLNANYIQVGNTRTIACQYPLQSQLESHFRMLAENRTPVL <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 11pt; text-decoration: none; vertical-align: baseline;">121 AVLASSSEIANQRFGMPDYFRQSGTYGSITVESKMTQQVGLGDGIMADMYTLTIREAGQK <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 11pt; text-decoration: none; vertical-align: baseline;">181 TISVPVVHVGNWPDQTAVSSEVTKALASLVDQTAETKRNMYESKGSSAVADDSKLRPVIH <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 11pt; text-decoration: none; vertical-align: baseline;">241 CRAGVGRTAQLIGAMCMNDSRNSQLSVEDMVSQMRVQRNGIMVQKDEQLDVLIKLAEGQG <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 11pt; text-decoration: none; vertical-align: baseline;">301 RPLLNSX

<span style="color: black; font-family: &#39;Courier New&#39;; font-size: 10pt;">32 bp long
@ xmM Mg2+ || 0 mM || 1.5 mM || 2 mM || 4 mM || 6 mM ||
 * Melting Temperature
 * (Degrees Celsius) || 64.6 || 71.5 || 72 || 72.9 || 73.3 ||

Reverse:
<span style="color: black; font-family: &#39;Courier New&#39;; font-size: 120%;">36 bp long @ xmM Mg2+ || 0 mM || 1.5 mM || 2 mM || 4 mM || 6 mM || GC content: 48.6%
 * TATCCACCTTTACTGTTAAGAGTTCAGCAGTGGACG ||
 * Melting Temperature
 * (Degrees Celsius) || 64.9 || 71.9 || 72.4 || 73.2 || 73.6 ||

Melting Temp
 * Melting Temperature @ mM Mg2+ || 0 mM || 1.5 mM || 2 mM || 4 mM || 6 mM ||
 * (Degree Celsius) || 61.2 || 68.6 || 69.1 || 70 || 70.5 ||

<span style="font-family: Verdana,sans-serif; font-size: 15px; line-height: 18px;">Expasy ProtParam Results:

 * Numbers of amino acids**: 306 **Molecular Weight:** 33512.8 kDa **Theoretical pI:** 8.99