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Hong's Research
Working on the virtual screening, no result yet. Working on the virtual screening, no result yet. Also, I worked on the transformation, but the result isn't come out right. There is no colony shows on the plate, which leads to start the accepting vector again. Lane 1: 1kb ladder Lane 2: pNIC as accepting vector Two bands are seemed, one band is very fainted. This means the input of DNA into pNIC Bsa4 is successful. Successfully put pNIC as accepting vector. Did not had a very good result, so redo. I will be working on the lab over the weekend. Finished PCR squared reaction and got a fairly good result in nanodrop. Started preparing pNIC-Bsa4 as accepting vector, the process was not successful which I did it three times. During the first time, the result is not valid because that I used different plasmid than the pNIC-Bsa4. Later, I did another accepting vector but someone changed the temperature of the water bath to 95 degree celsius which could kill the DNA in it. So the result was threw away. Finally, I did it correctly on Sunday and the result is acceptable.
 * __Week 11__**
 * __Week 10__**
 * __Week 9__**
 * __Week 8__**
 * __Week 7__**
 * __Week 6__**
 * __Week 5__**

I have redo my primer overlap and get a decent result that I can continue my research. Lane 1: Skip Lane 2: 100bp marker Lane 3: Primary PCR Lane 4: Secondary PCR
 * __Week 4__**

The gel doesn't look very good because I left the agarose on the table for too long. From this we can not tell if there is band in the primary PCR since I let it run for too long. So I redo the gel electrophoresis. Lane 1: Skip Lane 2: 100bp marker Lane 3: Primary PCR Lane 4: Secondary PCR

We see a clear band in the secondary PCR and a smear in the primary PCR. It is still hard to consider it is not contaminated. For the sake of time, I'll continue into Cloning.

This week, I have double check the results that I obtained from last week on Thursday. I couldn't get the image from the UV machine because the computer is not correctly formatted. I saved the gel into one of our fridges and will be check in the future. But the result is similar to the result from last week, which indicates that I have to start it over. So I guess I have to come on Saturday to obtain a reasonable result before the due date and make up my hours.
 * __Week 3__**

__**Week 2**__ Lane 1: 100bp marker Lane 2: Primary PCR Lane 3: Secondary PCR (There is a band in my primary PCR and no band in secondary PCR, I think this is possibly because I put secondary PCR into lane 2 and primary PCR into lane 3?)

Started Pymol Refresher that should be due in two weeks and finished it.

__**Week 1**__

Finished Primary Polymerase chain reaction and secondary chain reaction.

Hong - show us your latest and greatest research here. New gel pics and Weekly updates. - Dr. B

__**Summer Results:**__

Lane1 - Marker 100 bp Lane2 - Sample A Lane3 - Sample B Lane4 - Sample C Lane5 - Sample D

Lane-1: Marker 100bp Lane-2: Sample #1 Lane-3: Sample #2 Lane-4: Sample #3 Lane-5: Sample #4 Lane-6: Sample #5 Lane-7: Sample #6 Lane-8: Sample #7 Error in pipette out the samples, maybe it didn't work. Few days later, I went back to look at the samples and find out that they were evaporated. This means I can't start another gel electrophoresis because of time consuming.

Lane 1: Marker 100bp Lane 2: Sample A Lane 3: Sample B Lane 4: Sample C Lane 5: Sample D



Lane 1: Marker 100bp Lane 2: Sample A Lane 3: Sample B Lane 4: Sample C Lane 5: Sample D

Lane 1: Marker 100 bp Lane 2: Primary Reaction Lane 3: Secondary Reaction