Thioredoxin+Reductase+(Entamoeba+Hystolica)


 * *Target (protein/gene name): ** Thioredoxin Reductase


 * *NCBI Gene # or RefSeq#: **3410063


 * *Protein ID (NP or XP #) or Wolbachia#: ** EHI_155440


 * *Organism (including strain): ** Entamoeba Histolytica


 * Etiologic Risk Group (see link below): ** 2

Entamoeba Histolytica is the pathogenic amoeba which causes amebiasis, a parasitic infection which gives rise to (extra)intestinal diseases. Amebiasis is spread by fecal contamination (water, soil, improper sanitation) as well as through fecal-oral transmission during rectal inoculation or sexual exchange. An estimated 50 million cases occur every year, mainly in developing countries. Amebiasis can be asymptomatic, and it is predicted that as high as 25% of individuals in endemic places carry the amoeba antibodies. Though it affects individuals of all ages, mainly adult men are impacted by invasive amebiasis (it is suggested that this may be the result of differences in endocrinological processes between the sexes). A link between HIV/AIDS and amebiasis has also been made but the epidemiological impact of one on the other is disputed at this point in time.
 * Disease Information: **


 * [|Gene database link here] **

This protein reduces thioredoxin, which allows for correct folding of target proteins, and also reduces hydrogen peroxide (which is toxic to the organism).
 * Essentiality of this protein: **


 * Complex of proteins?: ** Multimer ("2-mer")

[|See this list]
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **


 * *EC#: **1.8.1.9


 * Link to BRENDA EC# page: **Brenda Page

Assay Kit (an E.Coli version is also available) NADP: N8035 SIGMA (5 mg per vial) = $174.00 6/6/2016 Edit: [|DTNB]
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **
 * Substrate**

**PDB#:** 4A5L
 * Structure (PDB or Homology model) **

Azelaic Acid in Staphylococcus aureus (strain Mu50)
 * Current Inhibitors: **


 * Expression Information (has it been expressed in bacterial cells): ** Yes, through E.Coli, engineered with a 6-His Tag


 * Purification Method : **Ni-NTA affinity column chromatography

>4CCQ:A|PDBID|CHAIN|SEQUENCE
 * Image of protein (PyMol with features delineated and shown separately): **
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code') **

MSNIHDVVIIGSGPAAHTAAIYLGRSSLKPVMYEGFMAGGVAAGGQLTTTTIIENFPGFPNGIDGNELMMNMRTQSEKYG

TTIITETIDHVDFSTQPFKLFTEEGKEVLTKSVIIATGATAKRMHVPGEDKYWQNGVSACAICDGAVPIFRNKVLMVVGG

GDAAMEEALHLTKYGSKVIILHRRDAFRASKTMQERVLNHPKIEVIWNSELVELEGDGDLLNGAKIHNLVSGEYKVVPVA

GLFYAIGHSPNSKFLGGQVKTADDGYILTEGPKTSVDGVFACGDVCDRVYRQAIVAAGSGCMAALSCEKWLQTH

>4CCQ:B|PDBID|CHAIN|SEQUENCE

MSNIHDVVIIGSGPAAHTAAIYLGRSSLKPVMYEGFMAGGVAAGGQLTTTTIIENFPGFPNGIDGNELMMNMRTQSEKYG

TTIITETIDHVDFSTQPFKLFTEEGKEVLTKSVIIATGATAKRMHVPGEDKYWQNGVSACAICDGAVPIFRNKVLMVVGG

GDAAMEEALHLTKYGSKVIILHRRDAFRASKTMQERVLNHPKIEVIWNSELVELEGDGDLLNGAKIHNLVSGEYKVVPVA

GLFYAIGHSPNSKFLGGQVKTADDGYILTEGPKTSVDGVFACGDVCDRVYRQAIVAAGSGCMAALSCEKWLQTH


 * Length of your protein in Amino Acids : ** 649


 * Molecular Weight: **  69827.7 kD


 * Molar Extinction coefficient of your protein at 280 nm wavelength: ** 59820 M^-1 cm^-1


 * TMpred graph Image **



ATGAGTAATATTCATGATGTTGTGATTATCGGCTCAGGACCTGCTGCACATACTGCGGCTATTTATTTAG GAAGATCTTCATTGAAACCAGTTATGTATGAAGGGTTTATGGCAGGAGGAGTAGCTGCAGGAGGACAACT AACTACCACTACTATCATTGAGAATTTCCCAGGATTTCCAAACGGTATTGATGGAAATGAATTAATGATG AATATGAGAACACAATCAGAGAAGTATGGAACTACTATCATTACAGAAACTATTGACCATGTTGACTTTT CAACACAACCATTTAAATTGTTTACTGAAGAAGGAAAAGAGGTACTTACAAAATCAGTTATTATTGCAAC AGGTGCTACAGCTAAAAGAATGCATGTTCCAGGAGAAGACAAATATTGGCAAAATGGAGTTAGTGCATGT GCCATTTGTGATGGGGCTGTTCCTATTTTCAGAAATAAAGTATTAATGGTAGTTGGTGGAGGAGATGCAG CTATGGAAGAAGCACTTCATTTAACAAAATATGGAAGTAAAGTTATTATTCTTCATCGAAGAGATGCTTT TAGGGCAAGTAAAACTATGCAAGAACGAGTATTAAATCATCCAAAGATTGAAGTAATATGGAATTCTGAA TTAGTAGAATTAGAAGGAGATGGTGATTTATTAAATGGGGCAAAAATTCATAATCTAGTTTCAGGAGAAT ACAAAGTTGTTCCAGTAGCTGGATTGTTTTATGCTATTGGACATAGTCCAAATAGTAAATTCTTAGGTGG ACAAGTTAAAACTGCTGATGATGGATATATTCTTACAGAAGGACCAAAAACTAGTGTTGATGGTGTATTT GCATGTGGGGATGTATGTGATAGAGTATATAGACAAGCTATTGTAGCTGCAGGAAGTGGTTGTATGGCAG CCTTAAGCTGTGAAAAATGGCTTCAAACTCATTAATTCAGTTTCTTTTTTAACA
 * *CDS Gene Sequence (paste as text only): **

***CDS Gene Sequence (codon optimized) - copy from** **output** **of** **Primer Design** **Protocol (paste as text only):** ***GC% Content for gene (codon optimized):**
 * *GC% Content for gene: **

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)

**
 * Primer design results for 'tail' primers (this is just 2 sequences): **