Target-+Carbonic+Anhydrase+(E.+coli)

//E. coli // carbonic anhydrase - (can or YadF) have sequence, assay ok, expression?, essentiality?

@http://ecogene.org/geneinfo.php?eg_id=EG12319

 forms Synthetic lethal with cynT....

**__Target: __** carbonic anhydrase- (can or YadF)
 * __Organism: __** E. coli

**__NCBI Gene # or RefSeq#: __**
**__EC#: __** 4.2.1.1

**__PDB # or closest PDB entry is using homology model: __**
1I6O- CRYSTAL STRUCTURE OF E. COLI BETA CARBONIC ANHYDRASE (ECCA) 1I6P 1T75

**CDS Gene Sequence: **
ATGAAAGACATAGATACACTCATCAGCAACAATGCACTATGGTCAAAAATGCTGGTGGAAGAGGATCCCGGGTTTTTTGAGAAACTGGCACAAGCGCAAAAACCGCGCTTTCTATGGATTGGATGTTCCGACAGTCGCGTTCCTGCAGAACGTTTAACCGGTCTTGAGCCGGGCGAACTCTTTGTTCACCGTAATGTTGCTAACCTGGTCATTCACACTGACCTGAACTGCCTTTCCGTGGTTCAGTATGCAGTGGATGTACTCGAAGTTGAACACATTATTATCTGTGGCCACTACGGTTGCGGCGGCGTACAAGCCGCAGTTGAAAACCCGGAACTGGGGCTTATCAACAACTGGCTGCTGCATATCCGCGATATCTGGTTCAAACATAGCTCATTGCTCGGCGAAATGCCGCAAGAGCGCCGTCTGGATACCTTGTGTGAACTGAACGTCATGGAACAGGTGTATAACCTGGGCCACTCCACCATTATGCAATCAGCGTGGAAACGCGGGCAGAAAGTTACCATTCACGGCTGGGCCTACGGCATTCACGACGGCTTGCTGCGTGATCTGGATGTTACCGCCACCAACCGCGAAACCCTTGAGCAACGTTACCGTCACGGGATTTCCAACCTCAAGCTGAAACACGCCAACCACAAATAA

**Amino Acid Sequence: ** >EG12319 Can Carbonic anhydrase, beta class MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSDSRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCGGVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGHSTIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHANHK

**length of your protein in Amino Acids ** 220 aa

=== **<span style="font-family: Arial,sans-serif; font-size: 10pt;">Molecular Weight of your protein in kiloDaltons using the **<span style="font-family: Arial,sans-serif; font-size: 10pt;">[|Expasy ProtParam] **<span style="font-family: Arial,sans-serif; font-size: 10pt;">website ** === 25096.7 daltons = ~25 kDa

ATGAAAGACATCGACACCCTCATCAGCAACAACGCGCTGTGGTCTAAAATGCTGGTTGAA GAAGACCCTGGTTTCTTCGAAAAACTGGCGCAGGCGCAGAAACCGCGTTTCCTGTGGATC GGTTGCTCTGACTCTCGTGTTCCGGCGGAACGTCTGACGGGTCTGGAACCGGGTGAACTG TTCGTTCACCGTAACGTTGCGAACCTGGTTATTCACACGGACCTGAACTGCCTGTCTGTT GTTCAATACGCGGTGGATGTTCTGGAAGTTGAACACATCATCATCTGCGGTCACTACGGT TGCGGTGGTGTTCAGGCGGCTGTTGAAAACCCGGAACTGGGTCTGATCAACAACTGGCTG CTGCACATCCGTGATATCTGGTTCAAACACTCTTCTCTGCTGGGTGAAATGCCGCAAGAA CGTCGCCTGGACACGCTCTGCGAACTGAACGTTATGGAACAGGTTTACAACCTGGGTCAC TCTACCATCATGCAGTCTGCGTGGAAACGTGGTCAGAAAGTTACCATCCACGGTTGGGCG TACGGTATTCACGACGGCCTGCTCCGCGACCTCGACGTCACTGCGACGAACCGCGAAACC CTGGAGCAGCGTTACCGTCACGGTATCTCTAATCTGAAACTGAAACACGCGAACCACAAA TAA
 * Primer Overlap Sequence for YadF:**

**<span style="font-family: Arial,sans-serif; font-size: 10pt;">Primer design for pNIC-Bsa4 cloning: ** <span style="font-family: 'Calibri','sans-serif'; font-size: 13.3333px;"><span style="font-family: Arial,sans-serif; font-size: 10pt;">Forward Primer: TACTTCCAATCCATG__AAAGACATCGACACCCT__ <span style="font-family: 'Calibri','sans-serif'; font-size: 13.3333px;"> <span style="font-family: 'Calibri','sans-serif'; font-size: 14.6667px;">Reverse Primer: TATCCACCTTTACTG__TTATTTGTGGTTCGCGTGT__ <span style="font-family: 'Calibri','sans-serif'; font-size: 14.6667px;">

= __<span style="font-family: Arial,sans-serif; font-size: 10pt;">E. coli carbonic anhydrase - cynT __ =

<span style="color: black; font-family: Arial,sans-serif; font-size: 10pt;">@http://ecogene.org/geneinfo.php?eg_id=EG10176

**__<span style="font-family: Arial,sans-serif;">Organism: __****<span style="font-family: Arial,sans-serif;"> E. coli ** **__<span style="font-family: Arial,sans-serif;">Target: __**<span style="font-family: Arial,sans-serif; font-size: 10pt;">carbonic anhydrase - cynT **__<span style="font-family: Arial,sans-serif;">NCBI Gene # or RefSeq#: __** **__<span style="font-family: Arial,sans-serif;">EC#: __**<span style="font-family: Helvetica,sans-serif; font-size: 11pt;">4.2.1.1

**__<span style="font-family: Arial,sans-serif;">PDB # or closest PDB entry is using homology model: __****<span style="font-family: Arial,sans-serif;"> N/A **
**__<span style="font-family: Arial,sans-serif;">Background/Disease: __**

**__<span style="font-family: Arial,sans-serif;">Image of protein (PyMol or etc): __****<span style="font-family: Arial,sans-serif;">N/A **
**__<span style="font-family: Arial,sans-serif;">CDS Gene Sequence: __** >EG10176 cynT Carbonic anhydrase, beta class GTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAACAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAACGCGGGCAATATCGTCCCTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCATATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCAAACTCATCCATCGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCCGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAA **__<span style="font-family: Arial,sans-serif;">Amino Acid __** **__<span style="font-family: Arial,sans-serif;">Amino acide Sequence: __** >EG10176 CynT Carbonic anhydrase, beta classMKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRLVPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCGAMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANLQTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTAA

**__<span style="font-family: Arial,sans-serif;">length of your protein in Amino Acids: __**<span style="font-family: Verdana,sans-serif; font-size: 8.5pt;">219 aa  **__<span style="font-family: Arial,sans-serif;">Molecular Weight of protein: __**<span style="font-family: Verdana,sans-serif; font-size: 8.5pt;"> 23764.26 daltons __**Primer overlap sequence of CynT from DNAworks:**__

ATGAAAGAAATCATCGACGGTTTCCTGAAATTCCAACGTGAAGCTTTCCCTAAACGCGAA GCGCTCTTCAAACAGCTGGCCACCCAGCAGTCTCCACGTACCCTGTTCATCTCTTGCTCT GACTCTCGTCTGGTACCGGAACTGGTTACCCAGCGTGAGCCGGGTGACCTGTTCGTTATC CGTAACGCGGGTAACATTGTTCCGTCTTACGGCCCAGAACCGGGTGGCGTTAGCGCGTCT GTTGAATACGCGGTTGCGGCTCTGCGTGTTTCTGACATCGTTATTTGCGGTCACTCTAAC TGCGGTGCGATGACCGCGATTGCCTCTTGCCAGTGCATGGACCACATGCCTGCAGTTTCT CACTGGCTGCGTTATGCGGATTCTGCACGTGTTGTTAATGAGGCGCGTCCACACTCTGAC CTGCCGTCTAAAGCGGCTGCGATGGTTCGTGAAAACGTTATTGCACAGCTCGCCAACCTG CAGACCCACCCGAGCGTTCGCCTGGCTCTGGAGGAAGGTCGTATCGCACTCCACGGTTGG GTTTACGACATCGAATCTGGTTCTATCGCGGCGTTCGATGGTGCGACCCGTCAGTTCGTT CCGCTGGCGGCGAACCCTCGTGTTTGTGCGATCCCTCTGCGCCAGCCGACTGCCGCGTAA

__**Primers for pNIC-Bsa4 cloning:**__ Forward primers: <span style="font-family: 'Calibri','sans-serif'; font-size: 13.3333px;">TACTTCCAATCCATG__AAAGAAATCATCGACGG__ Reverse primers: <span style="font-family: 'Calibri','sans-serif'; font-size: 14.6667px;">TATCCACCTTTACTG__TTACGCGGCAGTCGGCTGG__