Target+-+Pteridine+Reductase+1,+(L.+Major)

Is it a monomer or multimer as biological unit**? (make prediction at** @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): Predicted to be Multimer
 * Target (protein/gene name):** pteridine reductase 1
 * NCBI Gene # or RefSeq#:** LmjF23.0270
 * Protein ID (NP or XP #) or Wolbachia#:** Q01782
 * Organism (including strain):** Leishmania major
 * Etiologic Risk Group (see link below):** People in areas of North Africa, Middle East and some parts of China. It infects people who are exposed to sand flies or work outside and without fly net.
 * / Disease Information (sort of like the Intro to your Mini __Research Write__ up):**
 * Link to TDR Targets page (if present): []**
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.)**
 * Essentiality of this protein:** Essential in some stages of the organism.
 * Complex of proteins?:**Forms a complex of proteins
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):** Yes TDR targets give it a .7 druggability.

[]
 * *EC#: **1.5.1.33
 * Link to BRENDA EC# page:[]**
 * --** Show screenshot of BRENDA enzyme mechanism schematic
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):** Biopterin + NADPH(need to confirm)
 * -- link to Sigma (or other company ) page for assay (see Sigma links below):**
 * -- -or link (or citation) to paper that contains assay information**
 * -- links to assay reagents (substrates) pages.**
 * --- List cost and quantity of substrate reagents, supplier, and catalog #:** (Sigma $82.30 for 5 MG), maybe Cayman Chemical 10007662 $31 for 5 MG

-- PDB # or closest PDB entry if using homology model: 3H4V
 * Structure (PDB or Homology model)**

-- For Homology Model option:(no need for homology model because have original protein) Show pairwise alignment of your BLASTP search in NCBI against the PDB Query Coverage: Max % Identities: % Positives Chain used for homology:

[] MTAPTVPVALVTGAAKRLGRSIAEGLHAEGYAVCLHYHRSAAEANALSATLNARRPNSAI TVQADLSNVATAPVSGADGSAPVTLFTRCAELVAACYTHWGRCDVLVNNASSFYPTPLLR NDEDGHEPCVGDREAMETATADLFGSNAIAPYFLIKAFAHRVAGTPAKHRGTNYSIINMV DAMTNQPLLGYTIYTMAKGALEGLTRSAALELAPLQIRVNGVGPGLSVLVDDMPPAVWEG HRSKVPLYQRDSSAAEVSDVVIFLCSSKAKYITGTCVKVDGGYSLTRA ATGACTGCTCCGACCGTGCCGGTGGCGTTGGTAACAGGCGCCGCGAAGCGTCTTGGCCGC AGTATCGCTGAGGGACTCCACGCGGAGGGGTACGCTGTCTGCTTGCACTATCATCGCTCT GCTGCAGAAGCGAACGCACTATCCGCGACGCTCAACGCAAGGCGACCGAACAGCGCCATC ACGGTGCAGGCGGATCTGAGCAACGTTGCCACAGCCCCGGTCAGCGGCGCTGATGGCTCT GCACCTGTTACCCTCTTCACGCGCTGTGCTGAGTTGGTGGCTGCGTGCTACACCCACTGG GGACGCTGCGACGTGCTAGTGAACAACGCCTCTTCTTTCTACCCCACGCCGCTGCTGAGG AATGACGAGGATGGACACGAGCCCTGTGTCGGAGATAGAGAGGCAATGGAGACGGCCACC GCTGACCTCTTCGGCTCCAACGCGATAGCGCCCTACTTCTTGATTAAGGCGTTCGCGCAT CGCGTCGCGGGCACCCCAGCCAAGCATCGCGGCACCAACTACTCCATCATCAACATGGTC GACGCCATGACGAACCAGCCTCTTCTCGGGTACACCATATATACCATGGCCAAAGGGGCG TTGGAGGGGCTGACACGGTCTGCCGCGCTGGAGCTTGCGCCGCTGCAGATTCGAGTGAAC GGCGTTGGTCCGGGTTTGTCGGTGCTCGTCGATGACATGCCCCCTGCTGTGTGGGAGGGC CACCGCAGCAAGGTGCCTCTGTACCAGCGCGATTCCTCCGCCGCAGAGGTGAGCGACGTT GTTATCTTTCTGTGCTCCTCCAAGGCCAAGTACATCACCGGCACCTGTGTCAAAGTGGAT  GGTGGCTACAGCCTTACCCGGGCCTGA ATGACTGCGCCGACTGTCCCGGTTGCGCTGGTCACGGGTGCAGCGAAACGTCTCGGTCGT TCTATCGCCGAAGGTCTGCACGCGGAAGGTTACGCAGTTTGCCTGCACTATCACCGTAGC GCAGCGGAAGCGAATGCGCTGTCTGCGACGCTGAATGCACGTCGTCCGAACTCTGCGATC ACGGTACAGGCGGACCTGTCTAACGTTGCGACTGCCCCGGTTTCTGGTGCGGACGGTTCT GCCCCTGTTACCCTGTTCACCCGTTGCGCGGAACTCGTTGCGGCGTGCTACACCCACTGG GGTCGTTGTGACGTCCTGGTTAACAACGCGTCTTCTTTCTACCCGACGCCGCTCCTCCGT AACGACGAAGACGGTCACGAACCTTGCGTTGGCGACCGTGAAGCGATGGAAACCGCGACG GCGGATCTGTTCGGCTCTAACGCAATCGCGCCGTACTTCCTGATCAAAGCGTTCGCCCAC CGTGTTGCGGGTACTCCGGCGAAACACCGTGGTACTAACTACTCTATCATCAACATGGTT GACGCGATGACCAATCAGCCGCTGCTGGGTTATACCATCTACACCATGGCGAAAGGTGCG CTCGAGGGTCTGACTCGTTCTGCGGCGCTGGAACTGGCGCCTCTGCAAATTCGTGTTAAT GGTGTTGGTCCGGGTCTGTCTGTTCTCGTTGACGACATGCCACCGGCCGTTTGGGAAGGC CACCGCTCTAAAGTCCCGCTGTACCAGCGTGACTCTTCTGCTGCAGAAGTTTCCGATGTT GTTATCTTCCTGTGCTCTTCTAAAGCGAAATACATTACGGGTACCTGCGTTAAAGTTGAC <span style="background-color: #ffffff; font-family: courier,sans-serif; font-size: medium;">GGTGGTTACTCTCTGACCCGTGCGTAA
 * Current Inhibitors:** 25
 * Expression Information (has it been expressed in bacterial cells):** yes
 * Purification Method : n/a**
 * Image of protein (PyMol with features delineated and shown separately):**
 * (PDB ribbon image)**
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):**
 * length of your protein in Amino Acids:** 288aa
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website:** 30408.6
 * Molar Extinction coefficient of your protein at 280 nm wavelength:** 27765
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
 * CDS Gene Sequence (paste as text only):**
 * GC% Content for gene: 61.7 %**
 * CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):**


 * GC% Content for gene (codon optimized):** 57.5

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**
 * ( link to DNA Works output text file - **that should be saved in your Google Docs folder after you did the primer design protocol)

__Reverse Primer tail__ TAT CCA CCT TTA CTG TTA CGC ACG GGT CAG AGA GTA AC
 * Primer design results for 'tail' primers (this is just 2 sequences):**
 * [[image:reverseprimerdesignhwd.PNG width="633" height="479"]]

__Forward primer design__

TAC TTC CAA TCC ATG ACT GCG CCG ACT GTC