Leishmania+major+strain+Friendlin,+phosphatase+2c,+putative

code

code ***NCBI Gene** __ : ____ XP_001685487.1 __ ***Protein ID: 1A6Q, LmjF30.0380**
 * Target (protein/gene name): phosphatase 2c, putative **

***Organism (including strain): Leishmania major strain Friedlin**

**Leishmania is a protozoan and its parasite reposible for the disease leishmaniasis. It is spread to humans though bloodsucking sand flies. The symptoms of leishmaniasis are skin sores, which erupt weeks to months after a person is affected. The infection include fever, damage to the spleen and liver and anemia.**
 * Etiologic Risk Group (see link below): **
 * Essentiality of this protein: **
 * LmjF30.0380 ** has essentiality data

**Gene/Ortholog:** Tb927.6.1800 (OG4_19717); **Phenotype:** significant loss of fitness in bloodstream forms (3 days); **Source study:** alsford

**Gene/Ortholog:** Tb927.6.1800 (OG4_19717); **Phenotype:** no significant loss or gain of fitness in bloodstream forms (6 days); **Source study:** alsford

**Gene/Ortholog:** Tb927.6.1800 (OG4_19717); **Phenotype:** no significant loss or gain of fitness in procyclic forms; **Source study:** alsford

**Gene/Ortholog:** Tb927.6.1800 (OG4_19717); **Phenotype:** significant loss of fitness in differentiation of procyclic to bloodstream forms; **Source study:** alsford

**Druggable Target:yes**
 * Complex of proteins?:1 **

***EC#:** 3.1.3.16 **Link to BRENDA EC# page:** http://www.brenda-enzymes.org/php/result_flat.php4?ecno=3.1.3.16


 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **

**http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Enzyme_Assay/calcineurin.pdf**

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**-- List cost and quantity of substrate reagents and supplier**

**Structure Available (PDB or Homology model)**

http://www.pdb.org/pdb/explore/explore.do?pdbId=1a6q

PDB#: 1A6Q

**Current Inhibitors: none**

**Expression Information (has it been expressed in bacterial cells):** Escherichia coli


 * Purification Method: ** Purification is accomplished by ammonium sulfate fractionation, Sephadex G-75 chromatography, affinity chromatography on Amicon Matrex Gel Orange A and chromatofocusing.

**Image of protein (PyMol with features delineated and shown separately):**



amino acid sequence:

M GLMLPKPILSKVVDRAGNSFVNAACASQNGFRNSMEDAHMLVATDDADVAYFGIFDGHSNAECSAYVARELPQRLKKLPESITAEMLEKVCIEVDEAYMKTSTEGGTTGTFCVIRKDLTVTIANVGDSRILVCRGGKLIFATEDHKPYIPGETERIVACGGSVVSNRVDGDLAVSRAFGDASFKVKGTKDYRMQKVIAVPDVSVLQCEPNDFIILACDGVFEGNFSNEDVCQFVWEQQQNCWDDLAVVACRVCDEAIRCGSKDNISCLVVQLAEGASRVKAFGATSFVPGPPFPRNQQPCRAAYAQMAELGHTTTAAALQTRYQLLQAFSKNKLNSQPPVMRTAFEMSDEVDVETE WSFFGKGPAPGNEKNFFEALANTGNSS


 * Number of amino acids: ** 384


 * Molecular weight: ** 41732.2


 * Theoretical pI: ** 4.97




 * || atgggcctta tgctaccgaa accgatcctt agcaaggtcg tggatcgtgc agggaactct tttgtaaacg ctgcctgcgc ctcgcagaac ggctttcgca actccatgga ggatgcacac atgttagtcg ccaccgacga cgccgacgtg gcgtactttg gcatcttcga tgggcatagt aacgccgagt gtagcgccta tgtggcgagg gagctgccac agcggctgaa gaagctgccg gagtcgatca cggcggagat gttagagaag gtatgcatag aggtggatga ggcctacatg aagaccagca ccgagggcgg caccaccggc accttttgcg tcatccgcaa ggacctcaca gtgaccatcg ccaacgtcgg tgactcccgc atcctcgtct gccgcggcgg caaactgatt ttcgccactg aagaccacaa gccgtacatt ccgggggaga cggagcgcat cgttgcctgc ggcggcagtg tagtcagcaa ccgcgtcgac ggagaccttg ctgtatcgcg cgcctttggt gatgcgtcct tcaaggtaaa ggggacaaag gactaccgaa tgcagaaggt gattgccgtg cccgacgtgt cggtgctgca atgcgagccg aacgacttta tcattctcgc ctgtgacggc gtcttcgagg gcaacttctc caacgaggac gtttgccagt ttgtgtggga gcagcagcaa aactgctggg acgacttggc ggttgtggcg tgccgcgttt gcgatgaggc gatccggtgc ggcagcaagg acaacatctc ctgcttagtg gtgcagctgg ccgagggcgc ttccagggta aaggcattcg gcgccacatc cttcgtcccg gggccgccct ttccgcgcaa tcagcagccc tgccgcgccg cctacgcgca gatggcagag ctcggccaca ccacgacggc ggcagcgctg cagacgcgct accagcttct gcaggcgttc tccaagaaca agttgaattc gcagccgcct gtgatgcgaa ccgctttcga gatgagcgac gaggtggacg tggagacgga atggtccttc ttcggcaagg gccccgcgcc aggaaacgag aagaacttct ttgaggcgct cgccaatacg ggcaacagct cgtag ||

***GC% Content for gene:**

***CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):**

***GC% Content for gene (codon optimized):**

See **ProtocolTargetDiscoveryVDS.docx** for more
 * Resources: **

Etiologic Risk Group Categories (for pathogens): http://www.utexas.edu/research/rsc/ibc/agent_class.html#_Toc7238334

**Databases of genes/organisms:**

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