4-(cytidine+5'+-diphospho)-2-C-methyl-D-erythritol+kinase

** *Target (protein/gene name): ** 4-(cytidine 5' -diphospho)-2-C-methyl-D-erythritol kinase

 * *NCBI Gene # or RefSeq#: ** NCBI [|112699]


 * *Protein ID (NP or XP #) or Wolbachia#: ** [|Accession: CAN02442]


 * *Organism (including strain): **Bacillus anthracis, Strain: Ames Ancestor


 * *Etiologic Risk Group (see link below): ** Category A Priority Pathogens

The bacterium //Bacillus anthracis//, mostly found in soil, causes anthrax. One of the reasons this disease is considered so dangerous is because of the capability of the bacterial cells to behave like bacterial spores which can survive extremely harsh conditions. These spores can last up do decades and thus livestock, such as cows grazing a field, can easily take up these spores years after a previous outbreak has occurred. Anthrax occurs in three forms: cutaneous (affects skin), inhalational (in the lungs), and gastrointestinal (digestive tract). Anthrax can also be used as a bioterrorism weapon and has been developed to do so by many nations in the past. However, what actually kills people is not the bacterium, but the toxins the bacterium releases. One toxin is the poly-D-glutamic acid capsule and the other is simply termed the anthrax capsule. If left untreated, anthrax will almost certainly prove to be lethal.
 * * Disease Information (sort of like the Intro to your Mini __Research Write__ up): **


 * Link to TDR Targets page (if present): ** N/A


 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.): **[]

Is it a monomer or multimer as biological unit ** ? (make prediction at ** @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): 2 amino acid chains and 7 ligands
 * Essentiality of this protein: **


 * Complex of proteins? ** Yes
 * Druggable Target (cite evidence from a paper/database showing druggable in another organism): **[]
 * Druggable Target (cite evidence from a paper/database showing druggable in another organism): **[]


 * *EC#: ** 2.7.1.148


 * Link to BRENDA EC# page: **[]


 * __[[image:imageedit_2_5980963598.png]] __**

__** Enzyme Assay information **__ Bacteriophage of Bacillus cereus- anthracis, MHWa; PTA-4173--**$200.00**
 * -- Lik to Assay Information and Reagents: **[]
 * -- List cost and quantity of substrate reagents, supplier, and catalog #: **[]


 * PDB Number: ** 4M0C
 * Current Inhibitors: ** unknown
 * Expression Information (has it been expressed in bacterial cells): ** Yes, it has been expressed in M. tuberculosis
 * Purification Method : ** Purified by column chromatography on Whatman fibrous cellulose using 2-propanol, methanol, and water (in a ratio of 4:2:4) solution


 * Image of protein (PyMol with features delineated and shown separately): **



__**Figure 1.**__ The crystal structure of a FMN- dependent NADH-azoreductase from Bacillus anthracis str. Ames Ancestor in complex with FMN

SNA(MSE)NKTLIINAHPKVDDTSSVSIKVFKHFLESYKELISNN
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **

ETIEQINLYDDVVP(MSE)IDKTVLSAWEKQGNGQELTREEQKVT

ER(MSE)SEILQQFKSANTYVIVLPLHNFNIPSKLKDY(MSE)DN

I(MSE)IARETFKYTETGSVGLLKDGRR(MSE)LVIQASGGIYTN

DDWYTDVEYSHKYLKA(MSE)FNFLGIEDYQIVRAQGTAVLDPTE

VLQNAYKEVEEAASRLANKYIFSLEE


 * Length of your protein in Amino Acids: ** 237
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website: ** 27154.4 kDa
 * Molar Extinction coefficient of your protein at 280 nm wavelength: ** 28880 M-1 C-1
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.


 * *CDS Gene Sequence (paste as text only): **


 * *GC% Content for gene: **
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **
 * *GC% Content for gene (codon optimized): **