TargetF15+-+S-adenosylmethionine+Synthetase+(Burkholderia+pseudomallei)

Burkholderia pseudomallei is a gram negative, rod-shaped bacterium that causes the disease melioidosis. It is found in soil and water in areas such as Thailand and northern Australia. Signs and symptoms include pain in the chest, bones and joints, and it also causes lung nodules and pneumonia. This bacteria also causes Glanders, with formation of nodular lesions in the lungs and ulceration of the mucous membranes in the upper respiratory tract. http://www.ncbi.nlm.nih.gov/gene/945389 http://www.uniprot.org/uniprot/P0A817
 * *Target (protein/gene name): **S-adenosylmethionine Synthetase
 * *NCBI Gene # or RefSeq#: ** metK 945389
 * *Protein ID (NP or XP #) or Wolbachia#: ** NP_417417.1
 * *Organism (including strain): ** Burkholderia pseudomallei
 * Etiologic Risk Group (see link below): ** Risk group 2
 * */ Disease Information (sort of like the Intro to your Mini __Research Write__ up): **
 * Link to TDR Targets page (if present): **
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) **

Is it a monomer or multimer as biological unit ** ? (make prediction at ** @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): dimer
 * Essentiality of this protein: ** Involved in DNA methylation, and controls gene expression.
 * Complex of proteins?: **
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): ** 0.5 in M. tuberculosis

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0053851

http://www.brenda-enzymes.info/enzyme.php?ecno=2.5.1.6&Suchword=&organism%5B%5D=Escherichia+coli&show_tm=0 **ATP + L-methionine + H2O = phosphate + diphosphate +** S-adenosyl L - methionine
 * *EC#: ** 2.5.1.6
 * Link to BRENDA EC# page: **
 * -- ** ATP ** + ** [[image:L-met.png width="121" height="81"]]**+** H2O **= [[image:Phosphate.png width="62" height="72"]] + [[image:diphosphate.png width="114" height="67"]] + [[image:s-adenosylmethionine.png width="93" height="125"]]**


 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **
 * -- link to Sigma (or other company ) page for assay (see Sigma links below) **
 * -- -or link (or citation) to paper that contains assay information **
 * -- links to assay reagents (substrates) pages. **
 * --- List cost and quantity of substrate reagents, supplier, and catalog # **

-- PDB # or closest PDB entry if using homology model: 3IML (PDB) -- For Homology Model option: Show pairwise alignment of your BLASTP search in NCBI against the PDB Query Coverage: Max % Identities: % Positives Chain used for homology:
 * Structure (PDB or Homology model) **

MANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITT TANIDYIQIARDTIKRIGYDNTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGA GDQGLMFGYACDETPELMPLPIHLSHRLVERQANLRRDGRLPWLRPDAKSQVTVRYVDGK PHSIDTVVLSTQHAPEIDLPALREAVIEEVIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALI QVSYAIGVAEPTSVMVNTFGTGRVSDETITKLVREHFDLRPKGIIQMLDLLRPIYEKTAA YGHFGREEPEFSWEAADKALALAEAAGVEPAVQVAX ATGGCGAACGACTATCTGTTCACCTCTGAATCTGTTTCTGAAGGTCACCCGGACAAAGTT GCGGATCAAATCTCTGACGCGATTCTGGACGCAATCCTCGCGCAAGACAAATACTCTCGT GTTGCGGCGGAAACCCTGTGCAATACGGGTCTGGTTGTTCTCGCAGGTGAAATCACCACT ACCGCGAACATTGACTACATCCAAATTGCGCGTGACACCATCAAACGTATTGGTTACGAT AATACCGACTACGGTATCGACTACCGTGGTTGCGCGGTTCTCGTCGCGTACGACAAGCAA TCTCCGGACATCGCGCAGGGCGTTGACCGTGCGCATGACAATAATCTGGACCAGGGTGCG GGCGATCAGGGCCTGATGTTCGGTTATGCGTGCGACGAAACCCCAGAACTGATGCCGCTC CCGATCCACCTGTCTCACCGTCTGGTCGAACGTCAGGCGAACCTCCGCCGTGACGGTCGC CTGCCGTGGCTCCGTCCTGACGCAAAAAGCCAGGTTACCGTCCGTTACGTTGACGGCAAA CCGCATTCTATCGACACCGTTGTCCTGTCTACCCAGCACGCTCCGGAAATTGACCTGCCG GCTCTCCGTGAAGCGGTTATCGAAGAAGTTATCAAACCAACCCTCCCTGCCGACCTGATC AAAGGTGACATCAAATTCCTGGTGAATCCGACTGGCCGCTTCGTAATCGGTGGTCCGCAG GGTGACTGCGGTCTCACGGGTCGTAAAATCATTGTTGACACCTACGGTGGCGCAGCACCA CACGGTGGTGGTGCGTTCTCTGGTAAAGATCCGTCTAAAGTTGATCGTTCTGCGGCCTAC GCTGGTCGTTATGTAGCGAAAAACATCGTTGCAGCCGGTCTGGCGTCTCGTGCACTGATC CAGGTCTCTTACGCTATCGGCGTTGCCGAACCGACCTCTGTTATGGTTAATACCTTCGGT ACTGGTCGCGTTAGCGATGAAACCATTACCAAACTCGTTCGTGAACACTTCGACCTGCGC CCGAAGGGTATCATCCAGATGCTGGACCTGCTGCGTCCGATCTACGAAAAAACTGCTGCT TACGGTCACTTTGGTCGTGAAGAACCGGAATTCTCTTGGGAAGCTGCGGATAAAGCGCTC GCTCTGGCCGAGGCTGCGGGTGTTGAACCAGCGGTTCAGGTTGCGTAA ATGGCGAACGACTATCTGTTCACCTCTGAATCTGTT GAGATTTGATCCGCAACTTTGTCCGGGTGACCTTCAGAAACAGATTCAGAGGTGAACAGA ACAAAGTTGCGGATCAAATCTCTGACGCGATTCTGGACGCAATCCTCGCGCAAGACAAAT CAGACCCGTATTGCACAGGGTTTCCGCCGCAACACGAGAGTATTTGTCTTGCGCGAGGAT CCTGTGCAATACGGGTCTGGTTGTTCTCGCAGGTGAAATCACCACTACCGCGAACATTGA AACCAATACGTTTGATGGTGTCACGCGCAATTTGGATGTAGTCAATGTTCGCGGTAGTGG GACACCATCAAACGTATTGGTTACGATAATACCGACTACGGTATCGACTACCGTGGTTGC CGATGTCCGGAGATTGCTTGTCGTACGCGACGAGAACCGCGCAACCACGGTAGTCGATAC AAGCAATCTCCGGACATCGCGCAGGGCGTTGACCGTGCGCATGACAATAATCTGGACCAG CACGCATAACCGAACATCAGGCCCTGATCGCCCGCACCCTGGTCCAGATTATTGTCATGC CTGATGTTCGGTTATGCGTGCGACGAAACCCCAGAACTGATGCCGCTCCCGATCCACCTG CGTCACGGCGGAGGTTCGCCTGACGTTCGACCAGACGGTGAGACAGGTGGATCGGGAGCG AACCTCCGCCGTGACGGTCGCCTGCCGTGGCTCCGTCCTGACGCAAAAAGCCAGGTTACC ACGGTGTCGATAGAATGCGGTTTGCCGTCAACGTAACGGACGGTAACCTGGCTTTTTGCG CGCATTCTATCGACACCGTTGTCCTGTCTACCCAGCACGCTCCGGAAATTGACCTGCCGG GGGTTGGTTTGATAACTTCTTCGATAACCGCTTCACGGAGAGCCGGCAGGTCAATTTCCG CGAAGAAGTTATCAAACCAACCCTCCCTGCCGACCTGATCAAAGGTGACATCAAATTCCT GGACCACCGATTACGAAGCGGCCAGTCGGATTCACCAGGAATTTGATGTCACCTTTGATC GCTTCGTAATCGGTGGTCCGCAGGGTGACTGCGGTCTCACGGGTCGTAAAATCATTGTTG CGCACCACCACCGTGTGGTGCTGCGCCACCGTAGGTGTCAACAATGATTTTACGACCCGT ACACGGTGGTGGTGCGTTCTCTGGTAAAGATCCGTCTAAAGTTGATCGTTCTGCGGCCTA AGACCGGCTGCAACGATGTTTTTCGCTACATAACGACCAGCGTAGGCCGCAGAACGATCA ATCGTTGCAGCCGGTCTGGCGTCTCGTGCACTGATCCAGGTCTCTTACGCTATCGGCGTT CCAGTACCGAAGGTATTAACCATAACAGAGGTCGGTTCGGCAACGCCGATAGCGTAAGAG TGGTTAATACCTTCGGTACTGGTCGCGTTAGCGATGAAACCATTACCAAACTCGTTCGTG GCATCTGGATGATACCCTTCGGGCGCAGGTCGAAGTGTTCACGAACGAGTTTGGTAATGG CGAAGGGTATCATCCAGATGCTGGACCTGCTGCGTCCGATCTACGAAAAAACTGCTGCTT CCAAGAGAATTCCGGTTCTTCACGACCAAAGTGACCGTAAGCAGCAGTTTTTTCGTAGAT TGAAGAACCGGAATTCTCTTGGGAAGCTGCGGATAAAGCGCTCGCTCTGGCCGAGGCTGC TTACGCAACCTGAACCGCTGGTTCAACACCCGCAGCCTCGGCCAGAG Do Not Need this info for Spring (but still copy these lines to your Target page for now) [|DNA Works output] Forward: 5' TAC TTC CAA TCC ATG GCG AAA CAC CTC TTC ACG 3' Reverse: 5' TAT CCA CCT TTA CTG TTA TTT GAG ACC TGC GGC ATC 3' **
 * Current Inhibitors: **
 * Expression Information (has it been expressed in bacterial cells): ** E. coli
 * Purification Method : **
 * Image of protein (PyMol with features delineated and shown separately): **
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **
 * *length of your protein in Amino Acids: **396aa
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website: ** 42.7525kDa
 * Molar Extinction coefficient of your protein at 280 nm wavelength: ** 33600
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
 * *CDS Gene Sequence (paste as text only): **
 * *GC% Content for gene: **
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **
 * *GC% Content for gene (codon optimized): **
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * Primer design results for 'tail' primers (this is just 2 sequences): Ordered and designed by Marissa Hansen **