LUIS+V.

__ Results: __

Fig 1. Ampicillin coated agar plate with BL(DE3) E.coli without gbr22 plasmid.

Fig 2. Ampicillin coated agar plate with BL (DE3) E. coli transformed with gbr22 plasmid



Fig 3. BL(DE3) E. coli culture transformed with gbr22 plasmid after 24 hour incubation period.

Fig 4. Wet pellet of BL(DE3) E. coli transformed with gbr22 after being centrifuged at 5000 rpm at 4 degrees Celsius for 10 minutes.



Fig 5. Elution 1 and 2 containing purified gbr22 protein purified using Ni-NTA chromatography.



Fig 6. SDS PAGE wet gel of gbr22 protein. The leftmost well contains a protein ladder followed by cell lysate, soluble fraction, flow through, wash, elution 1, and elution 2 samples. The last three wells are wash, elution 1, and elution 2.



Fig 7. SDS PAGE dry gel of gbr22 protein. The leftmost well contains a protein ladder followed by cell lysate, soluble fraction, flow through, wash, elution 1, and elution 2 samples. The last three wells are wash, elution 1, and elution 2.