TargetF15- S-adeosylmethionine+syntheses(M.+tuberculosis)...............................................................................Ana+R.,+Bethany+R.

Target: S-adenosylmethionine synthetase(M. tuberculosis)

Organism:

M. tuberculosis

Prefix IDT Ordering: MtSAMs

Forward Tail Primer in 5' to 3' orientation:

5’ TACTTCCAATCCATGGTTTCTGAAAAAGGTCGT 3’

Reverse Tail Primer in 5' to 3' orientation:

5’ TATCCACCTTTACTGTTAGATCGCACGTTTGAGAT 3’

Disease/Relevance

Tuberculosis

Type (anhydrase, reductase, phosphatase, etc.): Synthase

E.C.

2.5.1.6 (methionine adenosyltransferase) same-same

structure:

<span style="font-family: arial,sans,sans-serif;">3TDE

<span style="color: #0070c0; font-family: arial,sans,sans-serif; font-size: 80%;">activity (from Brenda site)

<span style="font-family: arial,sans,sans-serif; font-size: 90%;">ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine

<span style="color: #0070c0; font-family: arial,sans,sans-serif; font-size: 80%;">assay

<span style="background-color: #ffffff; font-family: arial,sans,sans-serif;">Comparative protein modeling of methionine S-adenosyltransferase (MAT) enzyme from Mycobacterium tuberculosis: a potential target for antituberculosis drug discovery, shows essentiality

<span style="color: #0070c0; font-family: arial,sans,sans-serif; font-size: 80%;">Essentiality

<span style="background-color: #ffffff; font-family: arial,sans,sans-serif;">Comparative protein modeling of methionine S-adenosyltransferase (MAT) enzyme from Mycobacterium tuberculosis: a potential target for antituberculosis drug discovery, shows essentiality

<span style="color: #0070c0; font-family: arial,sans,sans-serif; font-size: 80%;">Druggability <span style="color: #0070c0; font-family: arial,sans,sans-serif; font-size: 80%;">0.5

<span style="color: #0070c0; font-family: arial,sans,sans-serif; font-size: 80%;">TDR Targets Link or EuPath, etc

<span style="color: #1155cc; font-family: arial,sans,sans-serif;">__@http://tdrtargets.org/targets/view?gene_id=6457__

<span style="color: #0070c0; font-family: arial,sans,sans-serif; font-size: 80%;">Gene (NOT gene ontology)

<span style="color: #1155cc; font-family: arial,sans,sans-serif;">Tuberculist / Rv1392

<span style="color: #0070c0; font-family: arial,sans,sans-serif; font-size: 80%;">protein/clone/DNA available

<span style="color: #0070c0; font-family: arial,sans,sans-serif; font-size: 80%;">substrate available?

<span style="color: #0070c0; font-family: arial,sans,sans-serif; font-size: 80%;">supplier

<span style="color: #0070c0; font-family: arial,sans,sans-serif; font-size: 80%;">Second Substrate

<span style="color: #0070c0; font-family: arial,sans,sans-serif; font-size: 80%;"># of inhibitors

<span style="color: #0070c0; font-family: arial,sans,sans-serif; font-size: 80%;">known inhibitors

<span style="color: #0070c0; font-family: arial,sans,sans-serif; font-size: 80%;">Notes B

<span style="color: #1155cc; font-family: arial,sans,sans-serif;">__@http://www.ncbi.nlm.nih.gov/pubmed/15670956__

<span style="color: #0070c0; font-family: arial,sans,sans-serif; font-size: 80%;">Notes D (a.a. size)

<span style="font-family: arial,sans,sans-serif;">261 aa

<span style="display: block; font-family: arial,sans,sans-serif; text-align: right;">0.5