Glucose-6-phosphate+dehydrogenase+(Plasmodium+falciparum)


 * Glucose-6-phosphate dehydrogenase //[P. falciparum]// **

**NCBI Gene # or RefSeq#:** **Gene ID: ****[|812093] **

**Protein ID (NP or XP #) or Wolbachia#:** **[|Accession: AAA65930.1] **

**Organism:** **//P. falciparum //****Strain: 3D7**

**Etiologic Risk Group: 2**

**Disease Information:** Malaria ended the lives of approximately 450,000 people in 2015, most of whom were children under the age of five. The disease causes symptoms such as accelerated heart rate, chills, fatigue, and fever which can be fatal to children. Majority of malaria cases come about through infection by //Plasmodium falciparum//, a protozoan parasite that is commonly transmitted through mosquitoes. Resistance to chloroquine, an anti-parasitic drug widely used to treat malaria, has caused a demand for the discovery and production of new drugs to combat the disease.

**Link to TDR Targets page (if present):** **N/A**

**Link to Gene Database page:** [|NCBI]; [|PlasmoDB]


 * Essentiality of this protein: ****[|Essential in pentose phosphate pathway and glutathione metabolism] **


 * <span style="font-family: Arial,sans-serif; font-size: 10pt;">Is it a monomer or multimer as biological unit?: two identical monomers **

<span style="font-family: Arial,sans-serif; font-size: 10pt;">**Complex of proteins?:** **<span style="font-family: Arial,sans-serif; font-size: 10pt;">No, dimer of two identical monomers **

<span style="font-family: Arial,sans-serif; font-size: 10pt;">**Druggable Target:** **<span style="color: #92d050; font-family: Arial,sans-serif; font-size: 10pt;">[|.2 in //L. major//] **


 * <span style="font-family: Arial,sans-serif; font-size: 10pt;">EC#: 1.1.1.49 **

<span style="font-family: Arial,sans-serif; font-size: 10pt;">**BRENDA EC# page:** <span style="font-family: Arial,sans-serif;"> http://www.brenda-enzymes.info/enzyme.php?ecno=1.1.1.49

<span style="font-family: Arial,sans-serif; font-size: 10pt;">Enzyme schematic of glucose-6-phosphate-dehydrogenase from BRENDA.

<span style="font-family: Arial,sans-serif; font-size: 10pt;">**Enzyme Assay information:**
 * **<span style="font-family: Arial,sans-serif; font-size: 10pt;">[|Sigma Aldrich Enzyme Assay Info] **
 * **<span style="font-family: Arial,sans-serif; font-size: 10pt;">Substrate pages: ****<span style="color: #92d050; font-family: Arial,sans-serif; font-size: 10pt;">[|glucose-6-phosphate dehydrogenase],[| NADP] **
 * <span style="font-family: Arial,sans-serif; font-size: 10pt;">G6PD human, recombinant, expressed in //E. coli//
 * o <span style="font-family: Arial,sans-serif; font-size: 10pt;">$385/ 100UG (SRP6505 SIGMA)
 * <span style="font-family: Arial,sans-serif; font-size: 10pt;">20 mM β-Nicotinamide adenine dinucleotide phosphate sodium salt hydrate
 * <span style="font-family: Arial,sans-serif; font-size: 10pt;">$89.80/ 100mg (N0505 SIGMA)
 * <span style="font-family: Arial,sans-serif; font-size: 10pt;">Other assay reagents: [|250 mM Glycylglycine (Buffer),] [|60 mM D-Glucose 6-Phosphate Solution (G-6-P)], [|300 mM Magnesium Chloride Solution (MgCl2)]

<span style="font-family: Arial,sans-serif;">**Structure (PDB or Homology model)**
 * BLASTP pairwise alignment:**

<span style="font-family: Arial,sans-serif;">-- Closest PDB# For Homology Model option: 2BH9

<span style="font-family: Arial,sans-serif;"> Query Coverage: 73%

<span style="font-family: Arial,sans-serif;"> Max % Identities: 35%

<span style="font-family: Arial,sans-serif;"> % Positives: 51%

<span style="font-family: Arial,sans-serif;"> Chain used for homology: N/A

<span style="font-family: Arial,sans-serif;">**Current Inhibitors: 94**

<span style="font-family: Arial,sans-serif;">**Expression Information: [|PubMed]; [|BRENDA]**

<span style="font-family: Arial,sans-serif;">**Purification Method: [|affinity column]**

<span style="font-family: Arial,sans-serif;">**Image of protein (for human 2BH9, G6PD not in PDB):** <span style="font-family: Arial,sans-serif;">

<span style="font-family: Arial,sans-serif;">**Amino Acid Sequence:** MNNYQNNYIYNEKTLDFINNDQDNDNLKYLKEYVYFTTTNQFDVRKRITVSLNLLANASSKIFLLNSKDK LDLWKNMLIKSYIEVNYNLYPATYLIDTSCTNENVNINNNNNNNNKNKNNYCYSNTTVISCGYENYTKYI EEIYDSKYALSLYSNSLNKEELLTIIIFGCSGDLAKKKIYPALFKLFCNNSLPKDLLIIGFARTVQDFDT FFDKIVIYLKRCLLCYEDWSISKKKDLLNGFKNRCRYFVGNYSSSESFENFNKYLTTIEEEEAKKKYYAT CYKMNGSDYNISNNVAEDNISIDDENKTNEYFQMCTPKNCPDNVFSSNYNFPYVINSILYLALPPHIFIS TLKKIIKKNCLNSKGTDKILLEKPFGNDLDSFKMLSKQILENFNEQQIYRIDHYLGKDMVSGLLKLKFTN TFLLSLMNRHFIKCIKITLKETKGVYGRGQYFDPYGIIRDVMQNHMLQLLTLITMEDPIDLNDESVKNEK IKILKSIPSIKLEDTIIGQYEKAENFKEDENNDDESKKNHSYHDDPHIDKNSITPTFCTCILYINSINWY GVPIIFKSGKGLNKDICEIRIQFHNIMGSSDENMNNNEFVIILQPVEAIYLKMMIKKTGCEEMEEVQLNL TVNEKNKKINVPEAYETLLLECFKGHKKKFISDEELYESWRIFTPLLKELQEKQVKPLKYSFGSSGPKEV FGLVKKYYNYGKNYTHRPEFVRKSSFYEDDLLDINY

<span style="font-family: Arial,sans-serif;">**Length of your** **<span style="font-family: Arial,sans-serif;">protein in Amino Acids: 736 aa **

<span style="font-family: Arial,sans-serif;">**Molecular Weight:** **<span style="font-family: Arial,sans-serif;">86358.6 Da **

<span style="font-family: Arial,sans-serif;">**Molar Extinction coefficient of your protein at 280 nm wavelength:** **<span style="font-family: Arial,sans-serif;">99115 ****<span style="font-family: Arial,sans-serif;">M^-1 cm^-1 **

<span style="font-family: Arial,sans-serif;">**TMpred graph Image** ( <span style="color: #0000ff; font-family: Arial,sans-serif;">http://www.ch.embnet.org/software/TMPRED_form.html <span style="font-family: Arial,sans-serif;">).

<span style="font-family: Arial,sans-serif;">

<span style="font-family: Arial,sans-serif;">**CDS Gene Sequence (paste as text only):** ATGGATTATGAGAATTTTGTAAAAAGTGCAGAAGAAATAAATAATTTACACAATGTAAATTATTTGGAAA CAAAAGACCTGAATGATTTTAATTGGAAAGCAGCTTATTATATATGTAAAGAAATATATGATAAGCAACA AATTAATAAAGATGGTTATGTTGTGATAGGTTTATCGGGAGGTCGAACTCCGATAGATGTTTATAAAAAT ATGTGTTTAATTAAAGACATTAAGATAGATAAATCTAAATTAATATTTTTTATTATTGATGAAAGGTATA AAAGTGATGATCATAAATTTAGTAATTATAATAATATAAAATTTTTATTTCATAATTTAAATATAAATGA AAAAGAACAATTATATAAACCTGACACTACCAAAAGTATTGTAGATTGTATATTAGATTATAATGACAAA ATAAAAATAATGATAGAGAAATATAAAAAAGTTGATATAGCAATATTAGGTATGGGTAGTGATTTCCATA TTGCCAGTTTATTTCCAAATATATTTTATAATATATATATGAATAACTATCAAAATAATTATATATATAA TGAAAAAACATTAGATTTTATAAATAATGATCAAGATAATGATAATTTAAAATATTTGAAAGAATATGTA TATTTTACGACAACAAATCAATTTGATGTTAGGAAAAGAATTACAGTATCTTTAAATTTATTAGCTAATG CATCAAGTAAAATATTTTTATTAAATTCTAAAGACAAATTAGATTTATGGAAAAATATGTTGATTAAATC ATATATTGAAGTGAATTATAATTTATATCCAGCTACTTATTTAATAGATACATCATGCACCAACGAAAAT GTTAATATTAACAATAACAACAATAATAATAATAAGAATAAGAATAATTATTGTTATAGTAATACCACTG TTATATCTTGTGGTTATGAAAATTATACAAAATCTATTGAAGAAATTTATGATTCTAAATATGCTCTATC TCTTTATTCTAATAGTTTGAATAAAGAAGAATTATTAACTATAATAATTTTTGGCTGTTCAGGTGATTTA GCCAAAAAAAAAATATATCCAGCTTTATTTAAATTATTTTGTAATAATTCCTTACCAAAAGATTTATTAA TCATTGGATTTGCTAGAACAGTTCAAGATTTCGATACATTTTTTGATAAAATAGTTATATATTTAAAACG ATGTTTATTATGTTATGAAGATTGGTCTATATCAAAAAAGAAGGATCTTTTAAATGGTTTTAAAAATAGG TGTCGATATTTTGTTGGTAATTATTCGTCTTCAGAAAGTTTTGAAAATTTTAATAAATATTTAACAACTA TTGAAGAAGAAGAAGCAAAAAAAAAATATTATGCAACATGTTATAAAATGAATGGTTCAGATTATAATAT ATCAAATAATGTTGCAGAGGATAATATTAGTATAGATGATGAAAATAAAACAAATGAATATTTTCAAATG TGTACTCCAAAAAATTGCCCTGATAATGTATTTTCATCAAATTATAATTTTCCATATGTTATAAATCGTA TGTTATATTTAGCATTACCTCCACATATATTTGTTAGTACTTTAAAAAATTATAAAAAAAATTGTTTAAA TAGTAAAGGCACTGATAAAATATTACTAGAAAAACCATTTGGAAATGATTTAGATTCATTTAAAATGTTA TCAAAACAAATATTAGAGAATTTTAATGAACAACAAATATATAGAATAGATCATTATTTGGGTAAGGATA TGGTTTCAGGATTGTTGAAATTAAAATTTACAAATACATTTTTATTATCTTTAATGAATAGACATTTTAT AAAATGTATTAAAATTACTCTTAAAGAAACTAAAGGTGTATATGGTAGAGGACAATATTTTGATCCCTAT GGTATTATTAGAGATGTTATGCAAAATCATATGTTACAATTATTAACATTAATAACTATGGAAGATCCTA TAGATTTAAATGATGAATCTGTAAAAAATGAGAAAATAAAAATTCTTAAATCAATTCCTTCGATCAAATT AGAAGATACTATTATTGGACAATATGAAAAAGCTGAAAATTTTAAAGAAGATGAAAATAATGATGATGAA TCGAAAAAAAATCATAGTTATCATGATGATCCACATATAGATAAAAATTCGATTACTCCAACATTTTGTA CATGTATCTTATATATTAATTCAATTAATTGGTATGGTGTACCAATCATTTTTAAATCTGGAAAAGGTCT GAATAAAGATATATGTGAAATACGTATACAATTCCATAATATTATGGGGTCGTCTGATGAAAATATGAAT AATAATGAATTTGTTATTATATTACAACCTGTTGAAGCTATATACCTAAAAATGATGATTAAAAAAACGG GTTGTGAAGAAATGGAAGAAGTACAATTAAACCTAACAGTGAATGAGAAAAATAAAAAAATTAATGTACC AGAAGCATATGAAACATTACTCTTAGAATGTTTTAAAGGACATAAAAAAAAATTCATCTCAGACGAGGAA TTGTATGAATCATGGAGAATATTTACTCCTTTACTTAAGGAACTCCAGGAAAAACAAGTCAAGCCTCTTA AATATTCTTTTGGATCATCAGGCCCTAAAGAGGTATTTGGACTTGTCAAAAAATATTACAATTATGGTAA AAATTATACGCACAGACCTGAGTTTGTTAGAAAATCCTCTTTTTATGAAGACGATTTGTTAGATATTAAT TGATATATGTATATATTTAAATTAACAAATTAACACCCAATGAATATGAAAATAATATATATATATATAT ATATATTATATGATTGTTTAGTATATTATTACCTATCTTTTATAAGATAACATAAATGTATATATTATGA CATATATATATATATATATATATTATTTCACTTATCTGCCCACGAACTTTATTTTTGTTTTAAAATTCTA GTATAATTAAATAAAAGAAAATATTTGGAACAATTTGCATTTTTTATGTATAAATAAAATTTATATAATA ATATACTTTCATACTTACTTTTTATTTTATTTTATTTTATTTATTTTTTAAATGTCTATTATATATACAT ATAAATGCGTTTTCAAATAAATAATAAAAACCCATGTTTAACTAATAATATTACAAA


 * <span style="font-family: Arial,sans-serif; font-size: 10pt;">GC% Content for gene: **

<span style="font-family: Arial,sans-serif; font-size: 10pt;">**CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):**

<span style="font-family: Arial,sans-serif; font-size: 10pt;">**GC% Content for gene (codon optimized):**