Low+Molecular+Weight+Phosphotyrosine+phosphatase (Vibrio+Cholerae)


 * Target (protein/gene name): **Low Molecular Weight Phosphotyrosine phosphatase

 ***NCBI Gene # or RefSeq#:** VC0395_A0559 VC395_1055

 ***Protein ID (NP or XP #) or Wolbachia#:** 4LRQ

 ***Organism (including strain):** Vibrio Cholerae

 **Etiologic Risk Group (see link below):** 2

 ***/****Disease Information** **(sort of like the Intro to your Mini ****Research Write ****up): **Vibrio cholerae is a virulent bacteria that causes Cholera, a diarrheal infection. There are 1.4 to 4.3 cases of cholera a year and 28,000 to 142,000 deaths due to cholera a year. Cholera is spread mainly through water that has been contaminated by fecal matter. Outbreaks of cholera tend to be explosive due to the short incubation period of the bacteria (2 hours to 5 days).


 * Link to TDR Targets page (if present): **N/A

[]
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) **


 * Essentiality of this protein: Yes **

Is it a monomer or multimer as biological unit**? (make prediction at ** <span style="font-family: Arial,sans-serif; font-size: 10pt;">@http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): 4 amino acid chains and 12 ligands in ASU

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Complex of proteins?: yes**

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):** []

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ***EC#:** 3.1.3.48

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Link to BRENDA EC# page:** [] <span style="font-family: Arial,sans-serif; font-size: 10pt;"> **--** Show screenshot of BRENDA enzyme mechanism schematic

<span style="font-family: Arial,sans-serif; font-size: 10pt;">**Enzyme Assay** **information (spectrophotometric, coupled assay ?, reagents):**

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **-- link to Sigma (or other****company****) page for assay (see Sigma links below)** @https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/ptp101bul.pdf

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **-- -or link (or citation) to paper that contains assay information**

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **-- links to assay reagents (substrates) pages.**

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **--- List cost and quantity of substrate reagents, supplier, and catalog #**

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Structure (PDB or Homology model)**

<span style="font-family: Arial,sans-serif; font-size: 10pt;">-- PDB # : 4LRQ

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Current Inhibitors:** <span style="background-color: #ffffff; color: #333333; font-family: 'Helvetica Neue',Helvetica,Arial,sans-serif; font-size: 14px;">3[N-MORPHOLINO]PROPANE SULFONIC ACID

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Expression Information (has it been expressed in bacterial cells):** yes

<span style="font-family: Arial,sans-serif; font-size: 10pt;">**Purification Method****:**

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Image of protein (PyMol with features delineated and shown separately):** <span style="font-family: Arial,sans-serif; font-size: 10pt;">

MQKVLVVCMGNICRSPTAEAVLRAKAAQLKVDVEVDSAGTIGYHQGNPPDARSKAAGEKRGYSFSGIKARKIRDEDFVKFDWILAADQENLAELKARCPQSHQHKLSLMLSHSDSEYQEIPDPYYGGERGFELVLDLVEDAAEQFLLKLKQQGQH <span style="font-family: Arial,sans-serif; font-size: 10pt;"> ***length of your protein in Amino Acids** 176 <span style="font-family: Arial,sans-serif; font-size: 10pt;">**Molecular Weight****of your protein in kiloDaltons using the****[|Expasy ProtParam]****website** code 17287.5 code <span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Molar****Extinction coefficient****of your protein at 280 nm wavelength:** code 12950 code <span style="font-family: Arial,sans-serif; font-size: 10pt;"> **TMpred graph Image** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it. <span style="font-family: Arial,sans-serif; font-size: 10pt;"> ***CDS****Gene Sequence****(paste as text only):**
 * <span style="font-family: Arial,sans-serif; font-size: 10pt; line-height: 1.5;">*Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ***GC% Content for gene:**

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ***CDS Gene Sequence (codon optimized) - copy from****output****of****Primer Design****Protocol (paste as text only):**

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ***GC% Content for gene (codon optimized):**

<span style="font-family: Arial,sans-serif; font-size: 10pt;">Do Not Need this info for Spring (but still copy these lines to your Target page for now)

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **(** **<span style="color: #008000; font-family: Arial,sans-serif; font-size: 10pt;">link to DNA Works output ****<span style="color: #008000; font-family: Arial,sans-serif; font-size: 10pt;">text file ****<span style="color: #008000; font-family: Arial,sans-serif; font-size: 10pt;">- **<span style="font-family: Arial,sans-serif; font-size: 10pt;">that should be saved in your Google Docs <span class="m9l40l772" style="font-family: Arial,sans-serif; font-size: 10pt;"> folder after you did the primer design protocol)

<span style="font-family: Arial,sans-serif; font-size: 10pt;">-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not <span class="apple-converted-space" style="font-family: Arial,sans-serif; font-size: 10pt;"> sure how to.

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Primer design results for 'tail' primers (this is just 2 sequences):**

<span style="font-family: Arial,sans-serif; font-size: 10pt;">**