Protein+Labs+SP13

Alyssa K and James T Aditi S and Nicolet F (pretty sure) Brendan C and Caroline C Kevin E and Shawn O Aaron H and Jensen G Madeline J
 * Mentors:**
 * Plates to save:**
 * Maybe save:**

List your bacterial yield here (final mg's- milligrams of total protein) for GBR22 there will be some kind of prize for the winner - of course your yield must be backed up by your data - e.g. Nandrop
 * Protein Yield Competition:**

85
 * || **Yield (mg)** ||  || ** Yield (mg) ** ||   || ** Yield (mg) ** ||
 * ARIEL || 0.47 || JENSEN || 0.940 || MUHAMMAD ||  ||
 * ALICE || 0.56 || JULIA || 0.763 mg at 574 nm || MELISSA || 0.757 ||
 * ANTONIO (Tony) || 1.02 || Jesus || 0.870 || NICOLET || 1.102 ||
 * AARON || 0.39 || JAMES || 1.586 || NANCY || 0.25 ||
 * ASHLEE ||  || JESSICA || 0.559 || NICOLE ||   ||
 * ALYSSA || 0.207 || JIAQI (Jackie) ||  || PRIYA || .42 ||
 * ALAKH (Okie) ||  || KARUNA ||   || RAMIRO ||   ||
 * ADITI || 0.25 || KHADY ||  || SHIVANI || 0.472 ||
 * ANITA ||  || KATHERINE (Renee) || 1.85 || STELLA || 0.558 ||
 * BRENDAN || 1.180 || KEVIN || 1.13 || Shelby (Dax) ||  ||
 * BRANDY || 0.996 || KATHERINE ||  || SHANNON ||   ||
 * CAROLINE || 0.279 || KEREN ||  || SELENA || 0.53 ||
 * D'ONDRIA ||  || KAVYA || 1.382 || SEO (Kelly) || 0.273 ||
 * DANIEL || 1.83 || KARINNA ||  || SHAWN ||   ||
 * EMILY || 0.996 || KELSEY ||  || SO-YOUN (So) ||   ||
 * FENG (Fin) || 0.310 || KEELY || .279 || SPENCER || 0.427 ||
 * GRAYSON ||  || MIGUEL ||   || SERENA || 0.927 ||
 * GRANT ||  || MARIANNA || 0.7 || HUNTER ||   ||
 * GRACE || 0.25 || MANUEL ||  || TRACY ||   ||
 * GAUTAM || 0.99 || Madeline ||  || VICTORIA (Vicky) || 0.427 ||
 * HYUN-YOUNG (Young) || 0.763 || MOHAMMAD (Qasim) || 0.477mg/L || WILLIAM (Will) ||  ||
 * IMRAN || 0.273 || MIN || 0.65 || WILLIAM ||  ||
 * JACQUELINE (Jacky) ||  ||   ||   ||   ||   ||

Online Lab Reports:
NOTE: For the Purification Lab Pictures - show your images of the Elutions 1 & 2, but only show the **Nanodrop image** for the Elution 1 at the **280 nm Wavelength only.** ARIEL C ALICE C ANTONIO G AARON H ASHLEE W ALYSSA K ALAKH R ADITI S ANITA V BRENDAN C BRANDY C CAROLINE C D'ONDRIA P DANIEL D EMILY J FENG G GRAYSON N GRANT T GRACE T GAUTAM W HYUN-YOUNG L(Young) IMRAN Z JACQUELINE E JENSEN G JULIA C Jesus De La O JAMES T JESSICA N JIAQI Z KARUNA A KHADY D KATHERINE F (Renee) KEVIN E KATHERINE V KEREN K KAVYA K KARINNA L KELSEY C KEELY W MIGUEL T MARIANNA U MANUEL Z MADELINE J MOHAMMAD Q A MIN S MUHAMMAD A MELISSA H NICOLET F NANCY I NICOLE W PRIYA P RAMIRO R SHIVANI B STELLA M Shelby F-G (Dax) SHANNON R SELENA I SEO K (Kelly) SHAWN O SO-YOUN P SPENCER C SERENA Z HUNTER T TRACY C VICTORIA G WILLIAM I WILLIAM E

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031013 - Need pics (from Expression part of the lab):

Alice, Alakh (Okie), D'Ondria, Grace, Gautam, Young, Jensen, Julia, Jesus (De La O), Jiaqi Z, Karuna, Renee (Katherine), Keren, Karinna, Kelsey, Miguel, Manuel, Muhammad A, Ramiro, Stella, Dax (Shelby), Kelly (Seo), Shawn, So P., Serena, Tracy, William I, William E.

Daniel - pics didn't work right

2013 Version:

**__Online Report: __**** use your page on the WikiSpaces (under Protein Labs) **

**__Lab Report: __**

This report will encompass the last three (3) labs: protein expression, purification, and characterization.

1,000 words total –

Since this is online – you must be efficient with your words! Make them count!

**Title: **think of something semi-creative

**Intro words: ** this should have some background information on technique of protein production and purification.

There is a good article here: Nat Methods. **2008** Feb;5(2):135-46. Protein production and purification.

Also a good website: []

End with an objective statement where you briefly sum-up what you set out to do in this set of experiments. Include a brief hypothesis.

**M&M 250 words **: be __succinct__, __concise__, __and brief__ while allowing an informed reader to be able to replicate your work. Do not show calculations for prelabs.

**<span style="font-family: Arial,sans-serif; font-size: 11pt;">Results **<span style="font-family: Arial,sans-serif; font-size: 11pt;">: show images of your results that you have already taken

**<span style="font-family: Arial,sans-serif; font-size: 11pt;">CAPTIONS **<span style="font-family: Arial,sans-serif; font-size: 11pt;">: make sure you have good captions (captions don’t count against the word limit)

**<span style="font-family: Arial,sans-serif; font-size: 11pt;">Transformation plates images - **<span style="font-family: Arial,sans-serif; font-size: 11pt;"> Mention how many colonies you got. **Flask images, purple pellet images (include the weight of your pellet)**

**<span style="font-family: Arial,sans-serif; font-size: 11pt;">Images of Elution 1 and 2 in tubes, Nanodrop screen shots (of 280nm reading for Elution 1 only) - **<span style="font-family: Arial,sans-serif; font-size: 11pt;">Include yields from your Nanodrop spectrophotometry for your protein amounts.

**<span style="font-family: Arial,sans-serif; font-size: 11pt;">Show brief calculations for Beer’s law calculations. A=Ebc **

**<span style="font-family: Arial,sans-serif; font-size: 11pt;">Protein Gel images **<span style="font-family: Arial,sans-serif; font-size: 11pt;">, **Image of Ladder from web as reference** (molecular weight marker used – get from website of company)

**<span style="font-family: Arial,sans-serif; font-size: 11pt;">Discusssion **

<span style="font-family: Arial,sans-serif; font-size: 11pt;">Analyze your results, address sources of error, and answer any questions from the handout


 * <span style="font-family: Arial,sans-serif; font-size: 11pt;">Q: **<span style="font-family: Arial,sans-serif; font-size: 11pt;"> Why do we use lysozyme? Why do we use Benzonase/Cyanase?


 * <span style="font-family: Arial,sans-serif; font-size: 11pt;">Q: **<span style="font-family: Arial,sans-serif; font-size: 11pt;"> Briefly, address how the HIS tag system works


 * <span style="font-family: Arial,sans-serif; font-size: 11pt;">Q: **<span style="font-family: Arial,sans-serif; font-size: 11pt;"> Explain what is in the sample after each step: Sample 1, 2, 3,4 ,5, and 6


 * <span style="font-family: Arial,sans-serif; font-size: 11pt;">Q: **<span style="font-family: Arial,sans-serif; font-size: 11pt;"> What is different about the Wash vs. the Elution buffer?


 * <span style="font-family: Arial,sans-serif; font-size: 11pt;">Q: **<span style="font-family: Arial,sans-serif; font-size: 11pt;"> State the size of your protein from the gel and compare it to the size you determined in the protein purification lab


 * <span style="font-family: Arial,sans-serif; font-size: 11pt;">Q: **<span style="font-family: Arial,sans-serif; font-size: 11pt;"> address the purity of your sample

**<span style="font-family: Arial,sans-serif; font-size: 11pt;">Conclusions **

<span style="display: block; font-family: arial,sans-serif; font-size: 11pt; text-align: justify;">Recap what was done

<span style="display: block; font-family: arial,sans-serif; font-size: 11pt; text-align: justify;">State key finding(s)

<span style="display: block; font-family: arial,sans-serif; font-size: 11pt; text-align: justify;">Address future directions/applications of this work to VDS research

**<span style="font-family: Arial,sans-serif; font-size: 11pt;">References (2-3) **

=Fun Plates:=