Target-+trehalose-phosphate+phosphatase+(Mycobacterium+tuberculosis)

NP_394665
 * Target (protein/gene name):** Trehalose-phosphate phosphatase
 * NCBI Gene # or RefSeq#: 16082598**
 * Protein ID (NP or XP #) or Wolbachia#:**

Risk Group 3 (RG3) @http://www.sciencedirect.com/science/article/pii/S0003986104000931
 * Organism (including strain):** Thermoplasma Acidophilum
 * Etiologic Risk Group (see link below):**
 * Background/Disease Information:** ​Thermoplasma Acidophilum is known to be one of the causes of diseases such as congenital heart disease and Parkinson's Disease.

Yes
 * Druggable Target:**

http://www.brenda-enzymes.org/php/result_flat.php4?ecno=3.1.3.12&Suchword=&organism[]=Mycobacterium+tuberculosis&show_tm=0 **+H20 >** ** +** @http://mic.sgmjournals.org/content/137/2/323.full.pdf+html []
 * *EC#: **3.3.1.12 ​
 * Link to BRENDA EC# page:**
 * **Reaction catalyzed by alkaline phosphatase (3.1.3.), -(3.13.12)** ||  ||
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**
 * -- link to Sigma (or other company) page for assay or assay reagents (substrates)**

@http://mic.sgmjournals.org/content/137/2/323.full.pdf+html
 * -- link (or citation) to paper that contains assay information**

-- PDB # or closest PDB entry if using homology model:1U02
 * -- List cost and quantity of substrate reagents and supplier**
 * HEPES**
 * 25 grams, $38.70**
 * Structure Available (PDB or Homology model)**

Diumycin Moenomycin
 * Current Inhibitors:**

[] []
 * Expression Information (has it been expressed in bacterial cells):**
 * Purification Method:**
 * Image of protein (PyMol with features delineated and shown separately):**

>1U02:A|PDBID|CHAIN|SEQUENCE MIEQSLEDALYAIVTQNPMIFLDYDGTLVPIIMNPEESYADAGLLSLISDLKERFDTYIVTGRSPEEISR FLPLDINMICYHGACSKINGQIVYNNGSDRFLGVFDRIYEDTRSWVSDFPGLRIYRKNLAVLYHLGLMGA DMKPKLRSRIEEIARIFGVETYYGKMIIELRVPGVNKGSAIRSVRGERPAIIAGDDATDEAAFEANDDAL TIKVGEGETHAKFHVADYIEMRKILKFIEMLGVQKKQ
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):**

247 Residues 27105 M-1 cm-1, at 280 nm measured in water
 * length of your protein in Amino Acids:**
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website:**
 * Molar Extinction coefficient of your protein at 280 nm wavelength:**
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.



ATGATAGAACAGTCACTTGAAGATGCACTTTATGCCATAGTAACGCAGAACCCCATGATATTCCTCGATT ATGACGGCACGCTTGTGCCGATAATAATGAACCCGGAGGAGAGCTATGCTGATGCTGGTCTCCTCTCGCT GATATCCGATCTTAAAGAAAGATTTGATACCTATATCGTTACGGGAAGATCTCCCGAGGAAATAAGCAGG TTTCTGCCACTTGACATAAATATGATCTGCTATCATGGCGCATGTTCGAAGATCAATGGGCAGATCGTTT ACAACAATGGATCAGATCGCTTCCTTGGAGTCTTCGACAGAATATACGAAGATACAAGGTCATGGGTATC TGATTTCCCGGGGCTCAGGATATATAGGAAGAATCTTGCAGTGCTGTATCATCTCGGACTTATGGGGGCG GATATGAAGCCAAAATTAAGATCGAGGATCGAAGAGATAGCCAGAATATTTGGAGTGGAAACATATTACG GGAAGATGATCATAGAGCTCCGTGTTCCCGGCGTTAACAAGGGATCTGCAATAAGGTCTGTAAGAGGAGA AAGGCCGGCCATAATAGCAGGCGATGATGCTACCGACGAGGCGGCCTTCGAAGCCAATGATGACGCGCTT ACAATAAAAGTCGGAGAAGGAGAAACGCACGCAAAGTTTCACGTGGCAGATTACATAGAAATGCGAAAAA TACTTAAGTTCATAGAAATGTTAGGAGTTCAGAAAAAGCAATAA
 * CDS Gene Sequence (paste as text only):**

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**
 * ( link to DNA Works output text file - **that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences):**