NEUSHA+R.

__Title:__ Protein Purification/Protein Characterization

__ Introduction: __

__ Materials & Methods: __

__ Results: __  Figure 4. Two 125 mL Erlenmeyer flasks containing PGEM-gbr22[G], AV LB Agar, Ampicillin, and E. Coli BL21 (DE3) indicating growth of bacteria producing purple protein after 24 hours in a shaking incubator operating at 37 degrees Celsius at 200-350 rpm.

 Figure 6. 50 mL conical tube containing resuspended purple protein pellet containing transformed bacteria cells (BL21 DE3) with plasmid (pGEM-gbr22[G]), PBS, and lysozyme.

Figure 8 and 8a. Trial 1 concentration reading of 0.06mg/ml and absorbance reading of 0.013 at 574nm and 0.021 at 280nm for Elution 1. Figure 9 and 9a. Trial 2 concentration reading of 0.07mg/ml and absorbance reading of 0.014 at 574nm and 0.024 at 280nm for Elution 1.  Figure 10 and 10a. Trial 1 concentration reading of -0.02mg/ml and absorbance reading of 0.005 at 574nm and 0.024 at 280nm for Elution 2.  Figure 11 and 11a. Trial 2 concentration reading of 0.01mg/ml and absorbance reading of 0.006 at 574nm and 0.018 at 280nm for Elution 2.

__ Discussion: __

__ Conclusions: __

__ References: __