Karuna+A.

RE Digest result gel: 6/26/12 VDS Lane 1- empty 2- 1 kb ladder 3-uncut plasmid 4- sample 1: EcoRI+Ne2buffer 5-sample 2: PvuII+ne2buffer 6- sample 3: ecoRI+PvuII+ne2buffer 7- sample 4- EcoRI+neb buffer The sample came out properly, with the weights accurately shown above.
 * Week 3/4:**

Pgbr22 Retry-3 & 4(successful!)

Pgbr22 PCR try 4:

Lane 1: 100 bp ladder Lane 2-5: PCR KJA samples+dye UV PCR pgbr22- sources of error: problems in making gel (agarose maybe not completely dissolved), issues in agarose itself, incorrect dilution Pgbr22 Unsuccessful Try 3: Lane 1: 1 Kb ladder Lane 2-5: PCR samples KJA

pmcherry plasmid growth My First (and Second) PCR-pgbr22 purple protein Midiprep of pmcherry
 * Week 2:**

DNA sequence still unavailable.



Dilution of primer: M13 Reverse (-27) 100 to 20 µ M 4 plates of LB Agar Plates made with Amp added Transformation Efficiency: pmcherry- 120ng/microL Colonies grown overnight, checked morning after, stored in incubator, then fridge. PCR with pGBR22; M13 Forward and ReversePrimers; Stored in freeze r
 * Week 1**