Summer+Plasmids

These will be the plasmids that you

Enter Information Regarding Plasmid Overnight Culture in LB media

NOTE: after you have Midi-prepped your culture to get your final plasmid, upload the yield to **GoogleDocs/Misc/Plasmid Concentrations** @https://docs.google.com/spreadsheet/ccc?key=0AoO2KqKh2q_-dENvNGJEdW9qdUxQZmFYeTVoeXA0SUE&usp=sharing Then send a sample to DNA Sequencing to verify authenticity of plasmid. -- once the plasmid is verified - move it to the regular 'plasmids' box (and but the other half in the top loading freezer in the 'plasmids' box) -- Update plasmid info on the Google 'Plasmid Concentrations' spreadsheet:

Low copy plasmids need **160 ml** of culture per person/midiprep: anything in pNIC-Bsa4 High copy plasmids only need **80 ml** of culture per person/midiprep: pGRB22, pGFP, pmCherry, pUC19


 * **Plasmid Name** || **Researcher Name(s)** || **Date** || **Cultured** **Bacterial** **Species** || **Volume of LB (ml)** ||
 * PNiC-BSa4 ||  ||   || E. coli DH5-alpha || 2x 160mL ||
 * pNIC-BSA4 ||  ||   || E.coli DH5alpha || 2x 160mL ||
 * FrTUHP in pnic-BSA4 ||  ||   || E. Coli DH5-alpha || 2x 160mL ||
 * pmcherry ||  ||   || E. Coli DH5-alpha || 160mL (each do 80) ||
 * YopH+pNic-BSSA4 ||  ||   || E. Coli DH5-alpha || 2x 160mL ||
 * PNiC-BSa4 ||  ||   || E. Coli DH5-alpha || 2x 160mL ||
 * puc19 ||  ||   || E.Coli DH5-alpha || 160mL (each grow 80 ml) ||
 * pGBR22 ||  ||   || E.Coli DH5-alpha || 160 mL (grow another 160 ml o/n so that you each have 160 ml) ||
 * pGBR22 ||  ||   || E.coli DH5-alpha || 80 mL ||
 * pGFP ||  ||   || E.coli DH5-alpha || 80 mL ||
 * pmcherry ||  ||   || E. coli DH5-alpha || 80 mL ||