Target+-+serine_threonine+protein+phosphatase+catalytic+subunit,+putative+(T.+cruzi)


 * Target (protein/gene name):** serine/threonine protein phosphatase catalytic subunit, putative
 * NCBI Gene # or RefSeq#:** EKF38512.1

http://www.ncbi.nlm.nih.gov/protein/EKF38512.1

(use **Summer13A** Plate for Oligos instead of ordering new ones - Dr. B 090313)) 091213 - also use the **GDocs/Misc/Primers/DNAWorks_OutputSummer13** to get your correct DNA Works file for **Tail** Primer Design
 * Protein ID (NP or XP #) or Wolbachia#:** enter this info - Dr. B 090313


 * Organism (including strain):** //Trypanosoma cruzi//
 * Etiologic Risk Group (see link below):**

http://www.utexas.edu/research/rsc/ibc/agent_class.html#_Toc7238334

TDRTargets Link: @http://tdrtargets.org/targets/view?gene_id=44419

//Chagas disease//
 * Background/Disease Information (sort of like the Intro to your Mini Research Write up):**


 * Essentiality of this protein:**
 * Gene/Ortholog:** Tb11.01.0450 (OG4_10262); **Phenotype:** significant loss of fitness in bloodstream forms (6 days); **Source study:** alsford


 * Complex of proteins?: No**
 * Druggable Target:** Yes, Druggability index (range: 0 to 1): 0.6


 * *EC#: 3.1.3.16 **


 * Link to BRENDA EC# page:** http://www.brenda-enzymes.org/php/result_flat.php4?ecno=3.1.3.16

Spectrophotometric.
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**


 * -- link to Sigma (or other company) page for assay or assay reagents (substrates)**


 * -- link (or citation) to paper that contains assay information**


 * -- List cost and quantity of substrate reagents and supplier**

-- PDB # or closest PDB entry if using homology model: ?? -- For Homology Model option:
 * Structure Available (PDB or Homology model)**

Query Coverage: 98% Max % Identities: 76% % Positives % Chain used for homology:


 * Current Inhibitors:**


 * Expression Information (has it been expressed in bacterial cells):**
 * Purification Method:**


 * Image of protein (PyMol with features delineated and shown separately):**

MSLDVDAIIDKLLGVRVSKPGKQVNLAENDVKNLALRSREILLAQPALLELEAPIKICGD IHGQYYDLLRLFDNGGFPPSANYLFLGDYVDRGKQGLETICLVLAFKVKFPENFFLLRGN HECASINRIYGFFDECKRRYNIRLWKAFTDTFNCLPVACIIDDKIFCCHGGLSPELQTMD QIKRIARPCDVTDAGLICDLLWSDPEEGLSGWGENDRGVSYTFGQDIVEKFLRKHEFDLI VRAHQVVEDGYQFFAKRQLITIFSAPNYCNEFDNSGAVMSVDADLLCSFQILKPSVKKPK FYQ
 * Amino Acid Sequence:**


 * length of your protein in Amino Acids:** 303 Amino Acids
 * Molecular Weight:** Predicted: 34417 Da
 * Molar Extinction coefficient of your protein at 280 nm wavelength:**
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html).




 * CDS Gene Sequence:**

ATGTCCCTCGACGTTGACGCGATCATCGATAAACTGCTGGGTGTTCGTGTTTCTAAACCG

GGTAAACAAGTTAATCTGGCAGAAAATGACGTTAAAAACCTGGCGCTGCGTTCTCGTGAA

ATCCTCCTCGCGCAGCCAGCCCTGCTGGAACTGGAAGCGCCGATCAAGATCTGCGGTGAC

ATCCATGGTCAGTACTACGATCTCCTCCGCCTCTTCGACAACGGTGGTTTCCCGCCGTCT

GCGAATTACCTGTTCCTGGGTGACTACGTCGACCGCGGCAAGCAGGGTCTGGAGACCATC

TGCCTGGTTCTGGCGTTCAAAGTTAAATTCCCGGAAAACTTCTTCCTGCTGCGTGGCAAC

CATGAATGCGCGTCTATCAACCGTATCTATGGTTTCTTCGATGAATGTAAACGTCGCTAC

AACATCCGCCTGTGGAAAGCGTTCACCGACACGTTCAACTGCCTGCCTGTTGCCTGCATT

ATCGACGACAAAATCTTTTGCTGCCACGGCGGTCTGAGCCCGGAACTGCAGACTATGGAT

CAGATCAAACGTATCGCGCGTCCGTGCGATGTTACCGATGCGGGTCTGATCTGTGACCTG

CTGTGGTCTGACCCGGAGGAAGGTCTGTCTGGTTGGGGTGAAAACGACCGTGGCGTTTCT

TATACCTTCGGTCAGGACATCGTTGAAAAATTCCTGCGTAAGCACGAGTTCGACCTGATC

GTTCGTGCGCATCAGGTTGTTGAAGACGGTTACCAGTTCTTCGCAAAACGTCAGCTCATC

ACCATCTTCTCTGCGCCGAACTATTGCAACGAATTTGACAACTCTGGCGCGGTTATGTCT

GTCGACGCGGACCTCCTGTGCTCTTTTCAGATCCTGAAACCGAGCGTGAAAAAACCGAAA

TTCTATCAGTAA


 * GC% Content for gene:** %

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**
 * ( link to DNA Works output text file - **that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences):**


 * References:**

DVGGDIMNNDYIFLGDYVDRGYNSVETFEYLLLLKLLFPKNITLLRGNHESRQITTVYGF 127