TargetF15+-+S-adenosylmethionine+Synthetase+(Mycobacterium+tuberuclosis)

**Target (protein/gene name)**:
S-adenosylmethionine synthetase

**NCBI Gene # or RefSeq#**:
Tuberculist: Rv1392 NCBI Gene: 83332 GeneID: [|886741]

https://www.ncbi.nlm.nih.gov/Structure/pdb/3TDE
 * Link to PubChem:**

**Protein ID (NP or XP #) or Wolbachia#**:
[|NP_215908]

**Organism (including strain):** //Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)//
Tuberculosis is an airborne disease that is spread from one person to another through the air. Symptoms of tuberculosis include coughing, chills, loss of appetite, fever, and coughing blood. Even though the bacteria usually attacks the lungs, it also can affect the kidney spine, and brain.
 * Disease Information: **

**Link to TDR Targets page (if present):** []
====**Link to Gene Database page** **([|NCBI], [|Tuberculist] ,[| BioCyc], [|TBDB]):** ====

https://www.ncbi.nlm.nih.gov/gene/885638

**Essentiality of this protein:**
It catalyzes the formation of S-adenosylmethionine (SAM) from Met and ATP. S-adenosylmethionine is an essential methyl donor in mycolipid biosynthesis. SAM synthetase is also involved in a subpathway involved in S-adenosyl-L-methionine biosyntheis which is part of the amino-acid biosynthesis pathway.  Contains ideR protein in its genes which is a metal regulator and when it is in the presence of iron, it can regulate transcription.

===**<span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">Is it a monomer or multimer as biological unit? (make prediction at @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): **=== <span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">This protein is a homotetramer. <span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">**Complex of proteins?**: No

====**<span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **<span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">0.5 From **TDR targets** ====

<span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">-- links to assay reagents (substrates) pages. [|Pyrophosphate Assay Kit]
 * <span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">-- link to [|Sigma] (or other company) page for assay (see Sigma links below) **
 * <span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">-- link (or citation) to paper that contains assay information: **[]

<span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">**List cost and quantity of substrate reagents, supplier, and catalog #**
__<span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">SIGMA: __ <span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">-Pyrophosphate assay kit (MAK168): $251.50

<span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">-Pyrophosphate reagent (P7275): $56.40 (1VL) $282.50 (10VL)

**<span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">Structure (PDB or Homology model): 3TDE **
<span style="font-family: Arial,Helvetica,sans-serif;">Current Inhibitors <span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">: (none for //M. tuberulosis//)

<span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">Expression Information (has it been expressed in bacterial cells):
AVA0421 vector (derived from pET14b and provides cleavable 6xHis tags at the N-termini of the expressed proteins). The recombinant plasmids were transformed into //E. coli// strain BL21(DE3)-R3-pRARE2 cells for expression testing.

<span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">Purification Method: Purified from 6xHis nickel-affinity tag
<span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">Image of protein (PyMol with features delineated and shown separately): <span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif; line-height: 0px; overflow: hidden;">

<span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">Amino Acid Sequence:
<span style="font-family: 'Times New Roman',serif; font-size: 16px;">GPGSMVSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETL <span style="font-family: 'Times New Roman',serif; font-size: 16px;">VTTGQVHVVGEVTTSAKEAFADITNTVRARILEIGYDSSDKGFDGATCGVN <span style="font-family: 'Times New Roman',serif; font-size: 16px;">IGIGAQSPDIAQGVDTAHEARVEGAADPLDSQGAGDQGLMFGYAINATPEL <span style="font-family: 'Times New Roman',serif; font-size: 16px;">MPLPIALAHRLSRRLTEVRKNGVLPYLRPDGKTQVTIAYEDNVPVRLDTVV <span style="font-family: 'Times New Roman',serif; font-size: 16px;">ISTQHAADIDLEKTLDPDIREKVLNTVLDDLAHETLDASTVRVLVNPTGKFV <span style="font-family: 'Times New Roman',serif; font-size: 16px;">LGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMRW <span style="font-family: 'Times New Roman',serif; font-size: 16px;">VAKNVVAAGLAERVEVQVAYAIGKAAPVGLFVETFGTETEDPVKIEKAIGE <span style="font-family: 'Times New Roman',serif; font-size: 16px;">VFDLRPGAIIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDKVDDLKRAI

===<span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif;">Length of your protein in Amino Acids: <span style="background-color: #ffffff; font-family: arial,sans,sans-serif;">__261__ aa ===

Molar Extinction coefficient of your protein at 280 nm wavelength
__ Ext. coefficient 30035 __ Abs 0.1% (=1 g/l) 0.691, assuming all pairs of Cys residues form cystines

__ Ext. coefficient 29910 __ Abs 0.1% (=1 g/l) 0.688, assuming all Cys residues are reduced

TMpred graph Image ([]):

CDS Gene:
ATGGTTTCTGAAAAAGGTCGTCTGTTTACGTCTGAGTCTGTTACGGAAGGCCATCCTGACAAAATTTGCGA CGCTATCTCTGACTCTGTACTGGATGCACTCCTGGCGGCAGACCCTCGTTCTCGTGTTGCGGTTGAGACTC TCGTAACCACCGGTCAGGTTCACGTTGTTGGTGAAGTTACCACCTCTGCAAAAGAAGCGTTTGCGGATATC ACTAACACGGTCCGTGCGCGTATTCTGGAAATTGGCTACGACTCTTCTGACAAGGGTTTCGATGGTGCGAC GTGCGGCGTGAACATCGGTATCGGTGCGCAGTCTCCTGATATTGCGCAGGGTGTTGACACCGCACACGAAG CGCGTGTGGAAGGTGCTGCAGATCCTCTGGACTCTCAGGGTGCCGGTGACCAGGGCCTGATGTTCGGTTAC GCAATCAATGCGACCCCTGAACTGATGCCGCTCCCGATCGCGCTGGCCCATCGCCTGTCTCGTCGTCTCAC TGAGGTTCGTAAAAACGGTGTTCTCCCGTACCTCCGCCCAGATGGTAAGACCCAGGTTACTATCGCGTACG AGGATAACGTTCCGGTTCGTCTCGACACTGTGGTAATCTCTACCCAGCACGCGGCTGACATCGACCTGGAG AAAACCCTCGATCCGGACATCCGCGAGAAAGTTCTCAACACTGTCCTGGATGACCTCGCACATGAGACGCT GGACGCATCCACCGTTCGTGTTCTGGTAAATCCTACGGGCAAATTCGTTCTGGGTGGTCCGATGGGTGATG CGGGTCTGACTGGTCGCAAGATCATCGTAGACACCTACGGCGGTTGGGCCCGTCACGGTGGTGGTGCGTTC AGCGGCAAAGACCCGTCTAAGGTTGACCGTTCTGCGGCCTATGCCATGCGTTGGGTTGCGAAAAACGTCGT GGCAGCAGGTCTGGCGGAGCGTGTAGAAGTTCAAGTCGCGTATGCAATCGGTAAAGCGGCACCTGTTGGTC TGTTCGTTGAAACTTTCGGCACCGAAACGGAAGATCCGGTAAAAATCGAAAAAGCGATTGGTGAGGTTTTT GATCTGCGTCCGGGTGCCATCATTCGTGATCTGAATCTGCTCCGTCCAATCTACGCGCCGACCGCAGCGTA CGGCCACTTTGGTCGTACCGACGTTGAACTGCCGTGGGAACAACTGGACAAGGTCGACGATCTCAAACGTG CGATCTAA

CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol:
1 ATGGTTTCTGAAAAAGGTCGTCTGTTTACGTCTGAGTC 2 ATAGCGTCGCAAATTTTGTCAGGATGGCCTTCCGTAACAGACTCAGACGTAAACAGACGA 3 TGACAAAATTTGCGACGCTATCTCTGACTCTGTACTGGATGCACTCCTGGCGGCAGACCC 4 ACCTGACCGGTGGTTACGAGAGTCTCAACCGCAACACGAGAACGAGGGTCTGCCGCCAGG 5 CGTAACCACCGGTCAGGTTCACGTTGTTGGTGAAGTTACCACCTCTGCAAAAGAAGCGTT 6 TTCCAGAATACGCGCACGGACCGTGTTAGTGATATCCGCAAACGCTTCTTTTGCAGAGGT 7 CGTGCGCGTATTCTGGAAATTGGCTACGACTCTTCTGACAAGGGTTTCGATGGTGCGACG 8 AATATCAGGAGACTGCGCACCGATACCGATGTTCACGCCGCACGTCGCACCATCGAAACC 9 GTGCGCAGTCTCCTGATATTGCGCAGGGTGTTGACACCGCACACGAAGCGCGTGTGGAAG 10 CCCTGGTCACCGGCACCCTGAGAGTCCAGAGGATCTGCAGCACCTTCCACACGCGCTTCG 11 GTGCCGGTGACCAGGGCCTGATGTTCGGTTACGCAATCAATGCGACCCCTGAACTGATGC 12 TGAGACGACGAGACAGGCGATGGGCCAGCGCGATCGGGAGCGGCATCAGTTCAGGGGTCG 13 GCCTGTCTCGTCGTCTCACTGAGGTTCGTAAAAACGGTGTTCTCCCGTACCTCCGCCCAG 14 CGGAACGTTATCCTCGTACGCGATAGTAACCTGGGTCTTACCATCTGGGCGGAGGTACGG 15 CGTACGAGGATAACGTTCCGGTTCGTCTCGACACTGTGGTAATCTCTACCCAGCACGCGG 16 CTCGCGGATGTCCGGATCGAGGGTTTTCTCCAGGTCGATGTCAGCCGCGTGCTGGGTAGA 17 ATCCGGACATCCGCGAGAAAGTTCTCAACACTGTCCTGGATGACCTCGCACATGAGACGC 18 TTGCCCGTAGGATTTACCAGAACACGAACGGTGGATGCGTCCAGCGTCTCATGTGCGAGG 19 CTGGTAAATCCTACGGGCAAATTCGTTCTGGGTGGTCCGATGGGTGATGCGGGTCTGACT 20 ACGGGCCCAACCGCCGTAGGTGTCTACGATGATCTTGCGACCAGTCAGACCCGCATCACC 21 GCGGTTGGGCCCGTCACGGTGGTGGTGCGTTCAGCGGCAAAGACCCGTCTAAGGTTGACC 22 GACGTTTTTCGCAACCCAACGCATGGCATAGGCCGCAGAACGGTCAACCTTAGACGGGTC 23 GTTGGGTTGCGAAAAACGTCGTGGCAGCAGGTCTGGCGGAGCGTGTAGAAGTTCAAGTCG 24 CGAACAGACCAACAGGTGCCGCTTTACCGATTGCATACGCGACTTGAACTTCTACACGCT 25 GCACCTGTTGGTCTGTTCGTTGAAACTTTCGGCACCGAAACGGAAGATCCGGTAAAAATC 26 CCGGACGCAGATCAAAAACCTCACCAATCGCTTTTTCGATTTTTACCGGATCTTCCGTTT 27 GTTTTTGATCTGCGTCCGGGTGCCATCATTCGTGATCTGAATCTGCTCCGTCCAATCTAC 28 GTCGGTACGACCAAAGTGGCCGTACGCTGCGGTCGGCGCGTAGATTGGACGGAGCAGATT 29 CCACTTTGGTCGTACCGACGTTGAACTGCCGTGGGAACAACTGGACAAGGTCGACGATCT 30 TTAGATCGCACGTTTGAGATCGTCGACCTTGTCCA

VSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETLVTTGQVHVVGEVT
TSAKEAFADITNTVRARILEIGYDSSDKGFDGATCGVNIGIGAQSPDIAQGVDTAHEARV EGAADPLDSQGAGDQGLMFGYAINATPELMPLPIALAHRLSRRLTEVRKNGVLPYLRPDG KTQVTIAYEDNVPVRLDTVVISTQHAADIDLEKTLDPDIREKVLNTVLDDLAHETLDAST VRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMR WVAKNVVAAGLAERVEVQVAYAIGKAAPVGLFVETFGTETEDPVKIEKAIGEVFDLRPGA IIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDKVDDLKRAI

Methionine added to beginning of sequence for E coli
MVSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETLVTTGQVHVVGE VTTSAKEAFADITNTVRARILEIGYDSSDKGFDGATCGVNIGIGAQSPDIAQGVDTAHE ARVEGAADPLDSQGAGDQGLMFGYAINATPELMPLPIALAHRLSRRLTEVRKNGVLPYL RPDGKTQVTIAYEDNVPVRLDTVVISTQHAADIDLEKTLDPDIREKVLNTVLDDLAHET LDASTVRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRS AAYAMRWVAKNVVAAGLAERVEVQVAYAIGKAAPVGLFVETFGTETEDPVKIEKAIGEV FDLRPGAIIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDKVDDLKRAI

Primer design results for 'tail' primers (this is just 2 sequences):
Forward Tail Primer in 5' to 3' orientation: 5’ TACTTCCAATCCATGGTTTCTGAAAAAGGTCGT 3’

Reverse Tail Primer in 5' to 3' orientation: 5’ TATCCACCTTTACTGTTAGATCGCACGTTTGAGAT 3’

<span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;"><span style="display: block; font-family: Verdana,sans-serif; font-size: 10.624px;"> Range 1: 1 to 403  Query_132989&tracks=[key:sequence_track,name:Sequence,display_name:Sequence,id:STD1,category:Sequence,annots:Sequence,ShowLabel:true][key:gene_model_track,CDSProductFeats:false][key:alignment_track,name:other%20alignments,annots:NG%20Alignments|Refseq%20Alignments|Gnomon%20Alignments|Unnamed,shown:false]&v=0:423&appname=ncbiblast&link_loc=fromHSP|Graphics Alignment statistics for match #1 ||~ Score <span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;"> <span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;">**Kalign of PDB, NCBI, and TubercuList amino acid sequences:** <span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;"> code <span style="background-color: #ffffff; color: #222222; font-size: 11.7px;" >3TDE        GPGSMVSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETLVTTGQVHV NP_215908    -MSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETLVTTGQVHV M            -VSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETLVTTGQVHV

3TDE         VGEVTTSAKEAFADITNTVRARILEIGYDSSDKGFDGATCGVNIGIGAQSPDIAQGVDTA NP_215908    VGEVTTSAKEAFADITNTVRARILEIGYDSSDKGFDGATCGVNIGIGAQSPDIAQGVDTA M            VGEVTTSAKEAFADITNTVRARILEIGYDSSDKGFDGATCGVNIGIGAQSPDIAQGVDTA

3TDE         HEARVEGAADPLDSQGAGDQGLMFGYAINATPELMPLPIALAHRLSRRLTEVRKNGVLPY NP_215908    HEARVEGAADPLDSQGAGDQGLMFGYAINATPELMPLPIALAHRLSRRLTEVRKNGVLPY M            HEARVEGAADPLDSQGAGDQGLMFGYAINATPELMPLPIALAHRLSRRLTEVRKNGVLPY

3TDE         LRPDGKTQVTIAYEDNVPVRLDTVVISTQHAADIDLEKTLDPDIREKVLNTVLDDLAHET NP_215908    LRPDGKTQVTIAYEDNVPVRLDTVVISTQHAADIDLEKTLDPDIREKVLNTVLDDLAHET M            LRPDGKTQVTIAYEDNVPVRLDTVVISTQHAADIDLEKTLDPDIREKVLNTVLDDLAHET

3TDE         LDASTVRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSA NP_215908    LDASTVRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSA M            LDASTVRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSA

3TDE         AYAMRWVAKNVVAAGLAERVEVQVAYAIGKAAPVGLFVETFGTETEDPVKIEKAIGEVFD NP_215908    AYAMRWVAKNVVAAGLAERVEVQVAYAIGKAAPVGLFVETFGTETEDPVKIEKAIGEVFD M            AYAMRWVAKNVVAAGLAERVEVQVAYAIGKAAPVGLFVETFGTETEDPVKIEKAIGEVFD

3TDE         LRPGAIIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDKVDDLKRAI NP_215908    LRPGAIIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDKVDDLKRAI M            LRPGAIIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDKVDDLKRAI code <span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;"> <span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;">**PDB Sequence:** <span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;">http://www.rcsb.org/pdb/explore/remediatedSequence.do?structureId=3tde <span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;">>3TDE:D|PDBID|CHAIN|SEQUENCE GPGSMVSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETLVTTGQVHVVGEVTTSAKEAFADITNTVR ARILEIGYDSSDKGFDGATCGVNIGIGAQSPDIAQGVDTAHEARVEGAADPLDSQGAGDQGLMFGYAINATPELMPLPIA LAHRLSRRLTEVRKNGVLPYLRPDGKTQVTIAYEDNVPVRLDTVVISTQHAADIDLEKTLDPDIREKVLNTVLDDLAHET LDASTVRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLAERV EVQVAYAIGKAAPVGLFVETFGTETEDPVKIEKAIGEVFDLRPGAIIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDK VDDLKRAI

https://www.ncbi.nlm.nih.gov/protein/15608531?report=fasta code >MSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETLVTTGQVHVVGEVTTSAKEAFADI TNTVRARILEIGYDSSDKGFDGATCGVNIGIGAQSPDIAQGVDTAHEARVEGAADPLDSQGAGDQGLMFG YAINATPELMPLPIALAHRLSRRLTEVRKNGVLPYLRPDGKTQVTIAYEDNVPVRLDTVVISTQHAADID LEKTLDPDIREKVLNTVLDDLAHETLDASTVRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGG GAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLAERVEVQVAYAIGKAAPVGLFVETFGTETEDPVKIEKAI GEVFDLRPGAIIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDKVDDLKRAI code
 * NCBI Sequence: THIS WAS USED TO DESIGN PRIMER (SUMMER 2017)**

http://tuberculist.epfl.ch/quicksearch.php?gene+name=Rv1392&submit=Search >M. tuberculosis H37Rv|Rv1392|metK VSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETLVTTGQVHVVGEVT TSAKEAFADITNTVRARILEIGYDSSDKGFDGATCGVNIGIGAQSPDIAQGVDTAHEARV EGAADPLDSQGAGDQGLMFGYAINATPELMPLPIALAHRLSRRLTEVRKNGVLPYLRPDG KTQVTIAYEDNVPVRLDTVVISTQHAADIDLEKTLDPDIREKVLNTVLDDLAHETLDAST VRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMR WVAKNVVAAGLAERVEVQVAYAIGKAAPVGLFVETFGTETEDPVKIEKAIGEVFDLRPGA IIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDKVDDLKRAI
 * TubercuList Sequence:**

<span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;"> <span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;"> code Query 6    VSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETLVTTGQVHVVGEVT  65 +SEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETLVTTGQVHVVGEVT Sbjct 1    MSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETLVTTGQVHVVGEVT  60
 * ~ Expect ||~ Method ||~ Identities ||~ Positives ||~ Gaps ||
 * 810 bits(2092) || 0.0 || Compositional matrix adjust. || 402/403(99%) || 403/403(100%) || 0/403(0%) ||

Query 66   TSAKEAFADITNTVRARILEIGYDSSDKGFDGATCGVNIGIGAQSPDIAQGVDTAHEARV  125 TSAKEAFADITNTVRARILEIGYDSSDKGFDGATCGVNIGIGAQSPDIAQGVDTAHEARV Sbjct 61   TSAKEAFADITNTVRARILEIGYDSSDKGFDGATCGVNIGIGAQSPDIAQGVDTAHEARV  120

Query 126  EGAADPLDSQGAGDQGLMFGYAINATPELMPLPIALAHRLSRRLTEVRKNGVLPYLRPDG  185 EGAADPLDSQGAGDQGLMFGYAINATPELMPLPIALAHRLSRRLTEVRKNGVLPYLRPDG Sbjct 121  EGAADPLDSQGAGDQGLMFGYAINATPELMPLPIALAHRLSRRLTEVRKNGVLPYLRPDG  180

Query 186  KTQVTIAYEDNVPVRLDTVVISTQHAADIDLEKTLDPDIREKVLNTVLDDLAHETLDAST  245 KTQVTIAYEDNVPVRLDTVVISTQHAADIDLEKTLDPDIREKVLNTVLDDLAHETLDAST Sbjct 181  KTQVTIAYEDNVPVRLDTVVISTQHAADIDLEKTLDPDIREKVLNTVLDDLAHETLDAST  240

Query 246  VRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMR  305 VRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMR Sbjct 241  VRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMR  300

Query 306  WVAKNVVAAGLAERVEVQVAYAIGKAAPVGLFVETFGTETEDPVKIEKAIGEVFDLRPGA  365 WVAKNVVAAGLAERVEVQVAYAIGKAAPVGLFVETFGTETEDPVKIEKAIGEVFDLRPGA Sbjct 301  WVAKNVVAAGLAERVEVQVAYAIGKAAPVGLFVETFGTETEDPVKIEKAIGEVFDLRPGA  360

Query 366  IIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDKVDDLKRAI  408 IIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDKVDDLKRAI Sbjct 361  IIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDKVDDLKRAI  403 code


 * SUMMER 2017: CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol:**

1 ATGTCTGAAAAGGGTCGCCTGTTCAC 26 2 TCGCAGATTTTGTCCGGGTGACCTTCCGTAACAGATTCGCTGGTGAACAGGCGACCCTTT 60 3 CCCGGACAAAATCTGCGACGCAATCTCTGACTCTGTACTGGATGCGCTCCTCGCGGCTGA 60 4 TGACCAGTAGTAACGAGCGTTTCAACCGCAACACGGGAACGTGGGTCAGCCGCGAGGAGC 60 5 ACGCTCGTTACTACTGGTCAGGTACACGTTGTTGGCGAAGTTACCACCTCTGCGAAGGAA 60 6 AGGATACGAGCGCGTACCGTATTCGTGATGTCGGCGAACGCTTCCTTCGCAGAGGTGGTA 60 7 GGTACGCGCTCGTATCCTGGAGATCGGTTACGACTCTTCTGATAAAGGCTTCGATGGTGC 60 8 AGGAGACTGCGCACCGATACCGATATTAACACCACACGTAGCACCATCGAAGCCTTTATC 60 9 CGGTGCGCAGTCTCCTGATATCGCGCAGGGTGTAGATACCGCACACGAAGCGCGTGTTGA 60 10 TTGGTCACCTGCACCCTGAGAGTCGAGCGGGTCTGCCGCACCTTCAACACGCGCTTCGTG 60 11 AGGGTGCAGGTGACCAAGGCCTGATGTTCGGTTATGCCATCAACGCGACCCCGGAACTCA 60 12 GAGACGGCGAGAGAGACGGTGGGCGAGGGCGATCGGCAGCGGCATGAGTTCCGGGGTCGC 60 13 CGTCTCTCTCGCCGTCTCACTGAGGTTCGTAAAAACGGTGTTCTGCCGTACCTGCGTCCT 60 14 GGGACGTTATCTTCGTACGCGATGGTAACCTGGGTTTTACCGTCAGGACGCAGGTACGGC 60 15 GCGTACGAAGATAACGTCCCGGTTCGTCTGGACACTGTTGTTATCTCTACCCAGCACGCA 60 16 CGGATATCTGGGTCCAGAGTTTTTTCCAGATCAATGTCCGCTGCGTGCTGGGTAGAGATA 60 17 ACTCTGGACCCAGATATCCGTGAAAAAGTTCTGAATACCGTCCTGGACGACCTGGCTCAC 60 18 TCGGATTAACCAGAACGCGAACGGTGGAGGCGTCCAGCGTCTCGTGAGCCAGGTCGTCCA 60 19 CGCGTTCTGGTTAATCCGACCGGTAAATTCGTACTGGGTGGTCCGATGGGTGACGCCGGT 60 20 GCCCAACCACCGTAGGTGTCTACAATGATTTTACGGCCGGTGAGACCGGCGTCACCCATC 60 21 ACCTACGGTGGTTGGGCCCGTCACGGTGGCGGTGCGTTTTCCGGCAAAGATCCGTCTAAA 60 22 TCGCAACCCAACGCATCGCATAGGCCGCAGAGCGATCAACTTTAGACGGATCTTTGCCGG 60 23 GATGCGTTGGGTTGCGAAAAATGTTGTTGCTGCGGGCCTCGCTGAACGCGTGGAAGTGCA 60 24 GAACAGACCAACTGGCGCTGCTTTACCAATTGCGTAGGCGACTTGCACTTCCACGCGTTC 60 25 GCGCCAGTTGGTCTGTTCGTCGAAACCTTTGGCACCGAAACCGAAGACCCGGTTAAGATT 60 26 CACCAGGGCGGAGGTCGAAAACTTCACCGATTGCTTTCTCAATCTTAACCGGGTCTTCGG 60 27 GACCTCCGCCCTGGTGCGATCATCCGCGACCTGAACCTGCTGCGCCCAATTTATGCGCCT 60 28 CAGTTCAACGTCCGTACGACCGAAATGACCATAAGCCGCGGTAGGCGCATAAATTGGGCG 60 29 TCGTACGGACGTTGAACTGCCGTGGGAACAGCTGGACAAAGTAGATGATCTCAAGCGTGC 60 30 TTAAATGGCACGCTTGAGATCATCTACT 28 <span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;"> <span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;">**SUMMER 2017 TAIL PRIMER DESIGN** <span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;">**Alexandra B.** <span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;">Forward: 5’ TACTTCCAATCCATG ATG TCTGAAAAGGGTCGCC 3' <span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;">Reverse: 5’ TATCCACCTTTACTG TTA AATGGCACGCTTGAGAT 3’  <span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;">** Haoyi W. ** <span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;"> Forward: 5' <span style="font-family: 'Times New Roman',serif; font-size: 12pt;">TACTTCCAATCCATG <span style="color: #ff0000; font-family: 'Times New Roman',serif; font-size: 10.5pt;">ATG <span style="font-family: 'Times New Roman',serif; font-size: 10.5pt;">TCTGAAAAGGGTCGC 3'  <span style="background-color: #ffffff; color: #222222; display: block; font-family: arial,sans-serif; font-size: 13.28px;"> Reverse: 5' TATCCACCTTTACTG <span style="color: #ff0000; font-family: 'Times New Roman',serif; font-size: 10pt;">TTA <span style="font-family: 'Times New Roman',serif; font-size: 10pt;">AATGGCACGCTTGAGA 3'