Phosphoglycerate+kinase+A+(Crithidia+fasciculata)

=Phosphoglycerate kinase, glycosomal (PGKA) =


 * *Target (protein/gene name): **Phosphoglycerate kinase, glycosomal (PGKA)


 * *NCBI Gene # or RefSeq#: N/A **
 * EC#: 2.7.2.3**


 * *Protein ID (NP or XP #) or Wolbachia#: 1QPG **


 * *Organism (including strain): //Crithidia fasciculata// **


 * Etiologic Risk Group (see link below): Risk Group 2 **

Subspecies of the African trypanosome, Trypanosoma brucei, which cause human African trypanosomiasis, are transmitted by the tsetse fly, with transmission-essential lifecycle stages occurring in both the insect vector and human host. During infection of the human host, the parasite is limited to using glycolysis of host sugar for ATP production. This dependence on glucose breakdown presents a series of targets for potential therapeutic development, many of which have been explored and validated as therapeutic targets experimentally. These include enzymes directly involved in glucose metabolism (e.g., the trypanosome hexokinases), as well as cellular components required for development and maintenance of the essential subcellular compartments that house the major part of the pathway, the glycosomes. []
 * */ Disease Information (sort of like the Intro to your Mini Research Write up): **

Link: []
 * Essentiality: **During infection of the human host, the parasite is limited to using glycolysis of host sugar for ATP production. This parasite's dependence on glucose breakdown presents a series of targets for potential therapeutic development, many of which have been explored and validated as therapeutic targets experimentally. These include enzymes directly involved in glucose metabolism (e.g., the trypanosome hexokinases and phosphoglycerate kinase), as well as cellular components required for development and maintenance of the essential subcellular compartments that house the major part of the pathway, the glycosomes.


 * Materials and Reagents**: N/A


 * Link to BRENDA EC# page: **

[]


 * -- ** Show screenshot of BRENDA enzyme mechanism schematic:


 * Structure (PDB or Homology model) **

-- PDB # or closest PDB entry if using homology model: 1QPG

-- For Homology Model option:

Show pairwise alignment of your BLASTP search in NCBI against the PDB

Query Coverage: N/A

Max % Identities: N/A

% Positives: N/A

Chain used for homology:

Druggable Target: N/A Assayable Enzyme Target: N/A

Link to Brenda: https://www.brenda-enzymes.org/enzyme.php?ecno=1.11.1.21&Suchword=&reference=&UniProtAcc=&organism%5B%5D=Mycobacterium+tuberculosis&show_tm=0 Structure:


 * Current Inhibitors: N/A **


 * Expression Information (has it been expressed in bacterial cells): Yes **


 * Purification Method : N/A **


 * Image of protein (PyMol with features delineated and shown separately): **




 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **

**Length of protein in amino acids: 415 **
**Molecular Weight**: **45356.61 kDa **


 * Molar Extinction coefficient of your protein at 280 nm wavelength: 1.67 M-1*cm-1 **


 * [[image:- phosphoglycerate kinase A.gif]] ||
 * [[image:- phosphoglycerate kinase A.gif]] ||

code format="genbank"  1 cacaacgact catctaataa cattctctcc atctcttccc ttcactcacg tgtgcgccat 61 ccgacacacc cgtctattcc ggtgcctgtt tctctctaca gaacctcctt tttcttgttc 121 agctggctgc ctcaggcact gcgctcgcac cccctccgcc gagccatgtc tctcgtgctg 181 aagaagagca tcgatgacgc caccgtcagg gacaagaagg tgctcatccg cgtggacttc 241 aacgtccccg tgaagaacgg taagatcacg aatgacttcc gcattcgctc cgcccttccg 301 acgatccaaa aggtgctgaa ggagggcggc tcctgcatcc tgatgagcca ccttggtcgg 361 ccgaagggtg cgagaatgag tgacccaagt ccggagaagg gtgtgcgcgg gtacgaggag 421 gccgccacgc tgcgcccggt tgctgcacgc atcagcgagc tgctggggca gaaggtggag 481 tttgcaccgg actgcctgga cgctgcgtcg tacgcgtcga agctgaagaa cgcggacgtg 541 ctgttgctgg agaacgtgcg tttctacgcg gaggagggca gcaagaagga ggaggagcgc 601 gacgcgatgg cgaaggtact agcgtcgtac ggcgacgtgt atgtcagtga cgcgtttggc 661 actgcgcacc gcgacagcgc gacgatgacg ggcatcccca aggtgctggg tgcgggctac 721 gccgggtacc tgatggagaa ggagatcaac tacttctcgc gagtgctgaa caacccgccg 781 <span class="ff_line">cggccgctgg ttgcgattgt cggcggtgcg aaggtcagcg acaagatcca gctgcttgac 841 <span class="ff_line">aacatgctgg gccgcatcaa ctaccttgtg attggtggcg cgatggcgta cacgttccag 901 <span class="ff_line">aaggcgcagg gccgcaagat cggcatctcg atgtgcgagg aggataagct ggaccttgcc 961 <span class="ff_line">aagtcgctgc tgaagaaggc gcaagagcgg aatgtgcagg tgtttctgcc agttgaccac 1021 <span class="ff_line">gtgtgcaaca aggaatttaa ggccgcggac tcgccgctgg tgacggagag cgtggacgtc 1081 <span class="ff_line">ccggacggct acatggcgct ggacattggc ccaaggacga tccacatgta cgaggaagtg 1141 <span class="ff_line">attggccgct gcaagagcgc gatctggaac ggcccgatgg gcgtgttcga gatgccgtgc 1201 <span class="ff_line">tactcgaagg gcacatttgc cgttgcgaag gcgatgggga ctggaacgca gaagaacggg 1261 <span class="ff_line">ctgatgagca tcatcggcgg tggtgacagc gcgagcgctg cggagctgag cggcgaggcg 1321 <span class="ff_line">aagaacatgt cgcatgtgtc caccggcggc ggtgcgtccc tggagctgct ggagggcaag 1381 <span class="ff_line">acgctgcctg gcgtcgccat tctgacggac aaggacgtga aggagcgggg agcttcgtgc 1441 <span class="ff_line">aagttcgctt tcggggtcgg gagcccgagc agggaggctt gtccgctccg ttgtgggcat 1501 <span class="ff_line">atctttggcg gtgcctccat cgtaagagag atagttaaga tcgtcgtcgc gctgctgatc 1561 <span class="ff_line">ggcattttta ttggtcgccg catgagcacc aagctgatcc ggtaaggtgt acgctgcgtc 1621 <span class="ff_line">tttttctctc cgtgcggaat ttcatctgag atgagcagca gcggcgccct tccggagaca 1681 <span class="ff_line">gcgaacgagt atcggcggcc acccaagcta cttgcgcctg gcactggcgc aatgattgtt 1741 <span class="ff_line">gttgctggca aaggatgcag gtgagtgggt gatggtggtg gagggcctgc ctgtctgtcg 1801 <span class="ff_line">ctctgtgtgt gtgtgtgtgt gtgcctcacg tactcagcac tcttctttct tcttcgcttt 1861 <span class="ff_line">ctagctcctc cttgtcgccc tcttgaagtc tgttgatgat gtacaagttt gtgtgttgtt 1921 <span class="ff_line">ggtcaccgca gcgtgagccg ttgtgcgctc ttcgtctacc tccgttctct ccgtctctac 1981 <span class="ff_line">aattttttct gtgtgtgtgt gtgtgaggaa gagagtgccg atgtgctgcg ccgtctcgac 2041 <span class="ff_line">ggaaagcgca gcaggtggca aaaacatgcg tgaagagctt gagtggcggc agctgcacag 2101 <span class="ff_line">aagcacttca gtcctcgagt gcgtgggcgt gtgctagttc acgggtattg gtgtgcgcga 2161 <span class="ff_line">ctcttttcct gcctgccgct gccgtttttt ttttttcaac ttcgaacgtc ctgacttcgc 2221 <span class="ff_line">cgctctagtc gtggcgctct cgaaggatcc code
 * *CDS Gene Sequence (paste as text only): **


 * *GC% Content for gene: 69.7% **

code format="genbank" <span style="background-color: #ffffff; font-family: monospace,serif;"> "MSLVLKKSIDDATVRDKKVLIRVDFNVPVKNGKITNDFRIRSAL PTIQKVLKEGGSCILMSHLGRPKGARMSDPSPEKGVRGYEEAATLRPVAARISELLGQ KVEFAPDCLDAASYASKLKNADVLLLENVRFYAEEGSKKEEERDAMAKVLASYGDVYV SDAFGTAHRDSATMTGIPKVLGAGYAGYLMEKEINYFSRVLNNPPRPLVAIVGGAKVS DKIQLLDNMLGRINYLVIGGAMAYTFQKAQGRKIGISMCEEDKLDLAKSLLKKAQERN VQVFLPVDHVCNKEFKAADSPLVTESVDVPDGYMALDIGPRTIHMYEEVIGRCKSAIW NGPMGVFEMPCYSKGTFAVAKAMGTGTQKNGLMSIIGGGDSASAAELSGEAKNMSHVS TGGGASLELLEGKTLPGVAILTDKDVKERGASCKFAFGVGSPSREACPLRCGHIFGGA SIVREIVKIVVALLIGIFIGRRMSTKLIR" code
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **


 * *GC% Content for gene (codon optimized): 69.7% **