Target-microcin+C7+self-immunity+protein+MccF+(Bacillus+anthracis)................Daniella+Perez

microcin immunity protein MccF Bacillus anthracis str. Ames Bacillus anthracis, more commonly known as anthrax, a bacteria normally found in the soil that can transform itself into a spore to survive years under the harshest conditions. Once these spores are in favorable conditions, they germinate and become thriving colonies and gain the ability to produce toxins which is what makes the bacteria such a potent killer in humans as well as animals. @http://www.ncbi.nlm.nih.gov/protein/BAR77315.1 Is it a monomer or multimer as biological unit**? (make prediction at** @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): Multimer yes with SELENOMETHIONINE
 * Target (protein/gene name):**
 * NCBI Gene # or RefSeq#:** 821575425
 * Protein ID (NP or XP #) or Wolbachia#:** BAR77315
 * Organism (including strain):**
 * Etiologic Risk Group (see link below):**
 * / Disease Information (sort of like the Intro to your Mini __Research Write__ up):**
 * Link to TDR Targets page (if present):** n/a
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.)**
 * Essentiality of this protein:**
 * Complex of proteins?:** No
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):**
 * *EC#: 4.2.1.11 **
 * Link to BRENDA EC# page:[|brenda-enzymes.org/enzyme.php?ecno=4.2.1.11]**
 * --** Show screenshot of BRENDA enzyme mechanism schematic



Expression organism: E. coli (BL21(DE3) magic Media Type: M9 minimal  Media volume: 5mL  Growth Temp: 37*C  Induction Temp: 18*C  Induction Reagent: 1 mM IPTG  Experiment type: assay
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**

[] **-- links to assay reagents (substrates) pages.** IPTG- [] E. coli- [] M9 Minimal solution- [] IPTG - $61.40 for 1G (16758) E. coli BL21 - $236.50 for 1 vial (GE27-1542-01) M9 Minimal Salts, 5x - $47.70 for 500mL (M9956) All supplied by Sigma-Aldrich
 * -- link to Sigma (or other company ) page for assay (see Sigma links below)**
 * -- -or link (or citation) to paper that contains assay information**
 * --- List cost and quantity of substrate reagents, supplier, and catalog #**
 * Structure (PDB or H**** omology model) **



>3GJZ:A|PDBID|CHAIN|SEQUENCE
 * Current Inhibitors:** There are 18 compounds
 * Expression Information (has it been expressed in bacterial cells):** It can be expressed in E. coli BL21 (DE3)
 * Purification Method :**
 * Image of protein (PyMol with features delineated and shown separately):**
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):**

SNAMPLPKSLKYGDTIGIYSPSSPVTYTSPKRFERAKSYLLQKGFHILEGSLTGRYDYYRSGSIQERAKELNALIRNPNV

SCIMSTIGGMNSNSLLPYIDYDAFQNNPKIMIGYSDATALLLGIYAKTGIPTFYGPALVPSFGEFEPFVDDTYKYFLETL

LHDQALPYNIKQPLFWSDEFINWEEKTKEKELRPNNWISVTNGQATGRVIGGNLNTIQGIWGSPYMPCIQEGDILFIEDS

SKDAATIERSFSFLKINGVFDKVSGIILGKHEQFDDCGTNRKPYEILLEVLQNQRIPLLADFDCCHTHPMITMPIGVQVK

MDATNKTIHILEKWKI


 * length of your protein in Amino Acids:** 336
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website-** 37984.4
 * Molar Extinction coefficient of your protein at 280 nm wavelength:** 52830
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
 * CDS Gene Sequence (paste as text only):**

 1 mstiidvyar evldsrgnpt vevevytesg afgraivpsg astgeheave lrdgdksryl  61 gkgvmnavnn vneaiapeiv gfdvtdqagi dramieldgt pnkgklgana ilgvsmavah  121 aaadfvglpl yrylggfnak qlptpmmnii nggshadnnv dfqefmilpv gaptfkesir  181 mgaevfhalk avlhdkglnt avgdeggfap nlgsnreale viieaiekag ykagenvflg  241 mdvassefyn ketgkydlag egrtgltsae mvdfyeelck dfpiisiedg ldendwdghk  301 llterigdkv qlvgddlfvt ntqklaegie kgisnsilik vnqigtltet feaiemakra  361 gytavvshrs getedatiad iavatnagqi ktgsmsrtdr iakynqllri edelgeiavy  421 dgiksfynik r
 * GC% Content for gene:**
 * CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):**
 * GC% Content for gene (codon optimized):**

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**
 * ( link to DNA Works output text file - **that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences):**