Targets+fructose-1,6-bisphosphate+aldolase+(P.+falciparum)


 * Target (protein/gene name):** fructose-1,6-bisphosphate aldolase
 * NCBI Gene # or RefSeq#:** PF3D7_1444800
 * Protein ID (NP or XP #) or Wolbachia#:**P14223 (from http://www.uniprot.org/uniprot/P14223)
 * Organism:** //Plasmodium falciparum//
 * Etiologic Risk Group (see link below):** RG2
 * Background/Disease Information:** P. falciparum is a protozoan parasite that is instrumental in causing malaria - the number one killer infectious disease in the world. The fructose-1,6-bisphosphate aldolase enzyme is a glycolytic enzyme which if targeted, could halt the production of ATP for the protozoan which would effectively kill it. The p. falciparum version of the enzyme also differs from the human version of the enzyme making selective targeting possible.
 * Essentiality of this protein:** Affects glycolysis so it is very essential to p. falciparum survivial (link to article: [])
 * Complex of enzymes:** no
 * EC#:** 4.1.2.13
 * Link to BRENDA EC# page:** []
 * --** Show screenshot of BRENDA enzyme mechanism schematic




 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**
 * -- link to Sigma (or other company) page for assay or assay reagents (substrates)**
 * -- link to paper that contains assay information:** http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_Information/2/aldolase.Par.0001.File.tmp/aldolase.pdf
 * -- List cost and quantity of substrate reagents and supplier**

From __Sigma__: (1) Trizma base, 25 g for $15.40 (2) D-Fructose 1,6-Diphosphate, Tetra(cyclohexylammonium) Salt, 5 g for $102.00 (3) ß-Nicotinamide Adenine Dinucleotide, Reduced Form, Disodium Salt, 50 mg for $22.60 __OR__ β-Nicotinamide adenine dinucleotide, reduced disodium salt, 15 vials with 5 mg per vial for $154.50 (4) a-Glycerophosphate Dehydrogenase/Triosephosphate Isomerase, 250 units at 75-200 units/mg for $48.40
 * Total reagent cost: $188.40 - $320.30 (depending on which reagent chosen for 3.)**


 * Structure Available (PDB or Homology model)**
 * -- PDB # or closest PDB entry if using homology model:** Direct match-[]
 * -- Query Coverage (if not direct match):** N/A
 * -- Max Ident (if not direct match):** N/A
 * Druggable Target (see Databases for this):** yes
 * Current Inhibitors:**
 * Expression Information (has it been expressed in bacterial cells):** expressed in E. coli
 * Purification Method:**
 * Image of protein (PyMol or etc):** see below
 * [[image:http://www.pdb.org/pdb/images/1a5c_bio_r_500.jpg caption="http://www.pdb.org/pdb/images/1a5c_bio_r_500.jpg"]] ||
 * http://www.pdb.org/pdb/images/1a5c_bio_r_500.jpg ||

MAHCTEYMNAPKKLPADVAEELATTAQKLVQAGKGILAADESTQTIKKRFDNIKLENTIE NRASYRDLLFGTKGLGKFISGAILFEETLFQKNEAGVPMVNLLHNENIIPGIKVDKGLVN IPCTDEEKSTQGLDGLAERCKEYYKAGARFAKWRTVLVIDTAKGKPTDLSIHETAWGLAR YASICQQNRLVPIVEPEILADGPHSIEVCAVVTQKVLSCVFKALQENGVLLEGALLKPNM VTAGYECTAKTTTQDVGFLTVRTLRRTVPPALPGVVFLSGGQSEEEASVNLNSINALGPH PWALTFSYGRALQASVLNTWQGKKENVAKAREVLLQRAEANSLATYGKYKGGAGGENAGA SLYEKKYVY
 * Amino Acid Sequence:**


 * length of your protein in Amino Acids =** 369 AA
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam]website =** 40105.0 Daltons
 * Molar Extinction coefficient of your protein at 280 nm wavelength:**

Ext. coefficient = 40255 Abs 0.1% (=1 g/l) 1.004, assuming all pairs of Cys residues form cystines Ext. coefficient = 39880 Abs 0.1% (=1 g/l) 0.994, assuming all Cys residues are reduced


 * CDS Gene Sequence:**

(Cannot seem to find gene sequence for p. falciparum)


 * GC% Content for gene:**

ATGGCGCACTGCACTGAGTACATGAATGCGCCGAAGAAACTGCCGGCTGACGTTGCGGAA GAACTCGCGACCACTGCGCAAAAACTGGTTCAGGCGGGTAAAGGTATCCTGGCGGCTGAT GAATCTACCCAAACCATCAAGAAACGCTTCGACAACATCAAGCTGGAAAACACCATCGAA AACCGTGCGTCTTACCGCGACCTGCTGTTCGGCACCAAAGGTCTGGGTAAATTCATCTCT GGTGCGATCCTGTTCGAGGAGACGCTGTTCCAGAAAAACGAAGCGGGCGTTCCAATGGTT AATCTGCTGCACAACGAGAACATCATCCCGGGTATCAAAGTTGACAAAGGCCTCGTAAAT ATCCCGTGCACCGATGAGGAAAAATCTACGCAGGGTCTCGACGGTCTGGCTGAACGTTGC AAAGAATACTACAAAGCTGGTGCTCGCTTTGCTAAATGGCGTACCGTTCTGGTTATCGAC ACGGCGAAGGGCAAACCGACCGACCTGAGCATCCACGAAACCGCGTGGGGCCTCGCACGT TACGCGTCTATCTGCCAACAGAATCGTCTGGTTCCGATCGTTGAACCGGAAATTCTGGCG GACGGCCCTCACTCTATCGAAGTTTGCGCGGTTGTTACGCAGAAGGTTCTGTCTTGCGTA TTCAAAGCTCTGCAAGAAAATGGTGTGCTGCTGGAAGGTGCTCTGCTGAAACCGAACATG GTTACGGCGGGTTACGAATGTACTGCGAAGACGACCACCCAGGACGTTGGTTTCCTGACC GTTCGTACGCTGCGTCGTACGGTTCCTCCGGCGCTGCCGGGTGTTGTATTCCTGAGCGGT GGTCAGTCTGAGGAAGAAGCATCCGTTAACCTGAATTCTATCAATGCGCTCGGTCCGCAC CCGTGGGCGCTCACGTTCTCTTATGGTCGTGCGCTGCAGGCGTCTGTTCTGAACACCTGG CAGGGTAAAAAAGAAAACGTGGCGAAAGCGCGTGAAGTTCTCCTGCAACGCGCTGAAGCG AACTCTCTGGCGACCTACGGTAAGTATAAAGGCGGTGCGGGTGGTGAAAACGCTGGCGCG TCTCTGTACGAAAAGAAGTACGTTTACTAA
 * CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol:**


 * GC% Content for gene (codon optimized):** 53.7%


 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**
 * link to DNA Works output text file : **https://docs.google.com/file/d/0B4O2KqKh2q_-UnllMWtWRVNESmM/edit

1 ATGGCGCACTGCACTGA 17 28 TTAGTAAACGTACTTCTTTTCGTACAGAGACGCGCCAGC 39
 * Primer design results for 'tail' primers (this is just 2 sequences):**