Target+-+Calcium+Bound+LIPL32+(pathogenic+Liptosperia)

= **Target:** __ **Calcium Bound LIPL32 (pathogenic //Leptospira//)** __= //Leptospira interrogans// is a a spirochete bacterium that can infect a variety of hosts, including dogs, rodents, and humans. It is spread through contact with the urine of an infected host. The contact can be direct or indirect, as through a standing body of water. Once contact has been made, the bacterium will enter the bloodstream and infected many different cells and organs, a process thought to be a result from //Leptospira'//s ability to attach to multiple receptors on the surface of a host's cell. It is commonly referred to as rat catcher's yellow as the host's body often turns yellow from jaundice during the course of the infection.
 * 1. NCBI Gene #: ** 2772192
 * 2. Protein ID (NP or XP #) or Wolbachia#: ** YP_001316.1
 * 3. Organism: ** //Leptospira interrogans serovar Copenhageni;// Strain: Fiocruz L1-130
 * 4. Etiologic Risk Group:** Risk Group 2 and Risk Group 3 (in Belgium)
 * 5. Background/ Disease Information : **

The organs most infected are the kidney and the liver, which can cause complications like renal failure, Weil's Syndrome, and kidney infection. During the first phase of infection, the host experiences vague symptoms like headache, muscle pain, fever, and nausea. It is only during the second infectious phase that the life-threatening symptoms, such as meningitis, fever, and organ failure, appear. When the body of the host starts to shut down, the bacterium will leave its host through the urine, after which it can survive in water for up to three months. //Leptospira// most often infects people that work with animals or work outdoors, especially in tropical climates, such as farmers, fish workers, and mine workers. Symptoms can last for a few days to a few weeks or even longer, although an early dose of doxycycline or penicillin will cure the illness. [] [] Lipl32 has been targeted in //Leptospira shermani,// another pathogenic strain of //Leptospira// in mice, and targeted this protein reduced the nuclear binding ability of the bacterium. []
 * 6. Link to TDR Targets page (if present): ** No TDR Targets Page present
 * 7. Link to Gene Database page: **
 * 8.Essentiality of this protein: ** This protein is essential to the function of the //Leptospira// organism because it is needed for the bacterium to interact with the extracellular matrix of the host cell, as well as modulating fibronectin binding.
 * 9.Complex of proteins?: ** No
 * 10.Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **
 * 11.EC#: ** 3.4.24.B14
 * 12. Link to BRENDA EC# page: **
 * 13. ** Show screenshot of BRENDA enzyme mechanism schematic



[] [] It has been expressed in //Liptosprira// cells.
 * 14. Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **
 * 14a.link to Sigma (or other company ) page for assay (see Sigma links below) **
 * 14b. links to assay reagents (substrates) pages. **
 * 15.List cost and quantity of substrate reagents, supplier, and catalog # **
 * 15a.Cost:** 421 USD
 * 15b. Quantity:** 1
 * 15c.Supplier:** SIGMA-ALDRICH
 * 15d. Catalog #**: D4943
 * 16. Structure Available: ** PDB #2WFK
 * 17. Current Inhibitors: ** None
 * 18. Expression Information (has it been expressed in bacterial cells): **
 * 19. Purification Method : ** X-Ray Diffraction
 * 20. Image of protein (PyMol with features delineated and shown separately): **
 * 21. Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **

RHNKYNSLTRIKIPNPPKSFDDLKNIDTKKLLVRGLYRISFTTYKPGEVKGSFVASVGLLFPPGIPGVSPLIHSNPEELQ
KQAIAAEESLKKAASDATK
 * 22. length of your protein in Amino Acids: ** 259 Amino Acids
 * 23. Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website: ** 28522.4 kDa
 * 24. Molar Extinction coefficient of your protein at 280 nm wavelength: ** 38390
 * 25. TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.

mkklsilais valfasitac gafgglpslk ssfvlsedti pgtnetvktl lpygsvinyy gyvkpgqapd glvdgnkkay ylyvwipavi aemgvrmisp tgeigepgdg dlvsdafkaa tpeeksmphw fdtwirverm saimpdqiak aakakpvqkl dddddgddty keerhnkyns ltrikipnpp ksfddlknid tkkllvrgly risfttykpg evkgsfvasv gllfppgipg vsplihsnpe elqkqaiaae eslkkaasda tk
 * 26. CDS Gene Sequence (paste as text only):**


 * 27. GC% Content for gene:** 8.0882352941176

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)

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 * Primer design results for 'tail' primers (this is just 2 sequences): **