Protein+tyrosine+phosphatase,+putative

Plasmodium vivax   is a protozoal parasite that is the   main source   of malaria. P. vivax is one of six species of malaria  carriers   that infect humans. http://plasmodb.org/plasmo/showRecord.do?name=GeneRecordClasses.GeneRecordClass&primary_key=PVX_099685
 * Target (   protein    / gene name ): Protein    phosphatase     -beta, putative ( P. vivax )**
 * NCBI Gene # or RefSeq#:** None available on PubChem
 * Protein ID (NP or XP #) or Wolbachia#:** 269949
 * Organism (including   strain    ):** //Plasmodium vivax //
 * Etiologic Risk Group (see link below):** R2
 * Background/   Disease Information    (sort of like the Intro to your MiniResearch Write up):**
 * Link to TDR Targets   page    (if present):** []
 * Link to Gene   Database    page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.)**
 * Essentiality of this protein:**
 * Gene/Ortholog:** cel8553 (OG4_10586); **Phenotype:** Sterility; **Source study:** neb
 * Gene/Ortholog:** cel8553 (OG4_10586); **Phenotype:** Growth  Defect   ; **Source study:** neb
 * Gene/Ortholog:** cel8553 (OG4_10586); **Phenotype:** Larval/Adult Lethal/Arrest; **Source study:** neb
 * Gene/Ortholog:** cel8553 (OG4_10586); **Phenotype:**  Embryonic   Lethal/Arrest; **Source study:** neb
 * Gene/Ortholog:** cel8553 (OG4_10586); **Phenotype:** Embryonic Lethal/Arrest; **Source study:** wormbase
 * Gene/Ortholog:** cel8553 (OG4_10586); **Phenotype:** Larval/Adult Lethal/Arrest; **Source study:** wormbase
 * Gene/Ortholog:** cel8553 (OG4_10586); **Phenotype:** Sterility; **Source study:** wormbase
 * Gene/Ortholog:** Tb927.3.1240 (OG4_10586); **Phenotype:** significant loss of   fitness    in bloodstream forms (3 days); **Source study:** alsford
 * Gene/Ortholog:** Tb927.3.1240 (OG4_10586); **Phenotype:** significant loss of fitness in bloodstream forms (6 days); **Source study:** alsford
 * Gene/Ortholog:** Tb927.3.1240 (OG4_10586); **Phenotype:** significant loss of fitness in procyclic forms; **Source study:** alsford
 * Gene/Ortholog:** Tb927.3.1240 (OG4_10586); **Phenotype:** no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms; **Source study:** alsford


 * Complex of proteins?: N/a**
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): 0.6**

[] http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Enzyme_Assay/calcineurin.pdf http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/General_Information/2/colipase.pdf -- PDB # or closest PDB entry if using homology model:
 * *EC#: 3.1.3.16 **
 * Link to BRENDA EC# page:** www.brenda-enzymes. org /php/result_flat.php4?ecno=3.1.3.16
 * --** Show screenshot of BRENDA enzyme mechanism schematic
 * Enzyme Assay   information (spectrophotometric, coupled assay ?, reagents ):**
 * -- link to Sigma (or other   company    ) page for assay (see Sigma links below)**
 * -- -or link (or citation ) to paper that contains assay information**
 * -- links to assay reagents (substrates) pages****.**
 * --- List cost and quantity of substrate reagents, supplier, and catalog #**
 * Supplier: Sigma Aldrich **
 * Catalog:**
 * Price: $50**
 * Structure Available (PDB or Homology model)**
 * [[image:vdsstream/4JNB.PNG caption="4JNB.PNG"]] ||
 * PDB x- ray crystallography of 4JNB E.C # 3.1.3.16 ||

-- For Homology Model option: Show pairwise   alignment    of your BLASTP search in NCBI against the PDB Query Coverage: 100% Max % Identities: 100% % Positives: 100% Chain used for homology: Chain A

MSNCLAPSPDCQLSDALNSKGNPTRPNESSERSERSEIGQSGQSIQNSQSNRNSQNDNSY MEKKKKERRSEKKTHSPEQVRQKGEKNYEQSEKKKKLSNDEGVQVSTCETFVNEDNYTTY ENLSVKELSDEEVFSENEYNKKIKRSQNSLLISKEFYDFLVSNDDDQVSEKCTKNDARFE ELLQSEANSPPATCSTKVSHNVDEWINKLLKCELLHIEEVKLMCTLLRDILKEEPNCVQV SVPVTVAGDIHGQFYDLLELFHIGGFPPDVNYLFLGDYVDRGYYSCECFCLVACLKIKYP SRVTILRGNHESRQITKVYGFYDECMRKYDGDYNAWRYITDAFDYLPLTAIISNQIFCDH GGISPYLHTINQINNLDRFKEIPQDGPICDLLWSDPAGPEDGIIEGWKASPRGAGVVFSE ERTNAFLRLNKLSCICRAHQLVQDGFLWMHNDKVVTIFSAPNYCYRCGNSASLMLVDEYM EKDFVTFNTAPLRANAKALRRNVGYML Ext. coefficient 62895 Abs 0.1% (=1 g/l) 1.084, assuming all pairs of Cys residues form cystines Ext. coefficient 61770 Abs 0.1% (=1 g/l) 1.064, assuming all Cys residues are reduced MLEVQPGLYFGGAAAVAEPDHLREAGITAVLTVDSEEPSFKAGPGVEDLWRLFVPALDKPETDLLSHLDRAVAFIGQARA EGRAVLVHSHAGVSRSVAIITAFLMKTDQLPFEKAYEKLQILKPEAKMNEGFEWQLKLYQAMGYEVDTSSAIYKQYRLQK VTEKYPE Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Current Inhibitors:**
 * Expression Information (has it been expressed in bacterial cells):**
 * Purification Method   :**
 * Image of protein (PyMol with features delineated and shown separately):**
 * Amino Acid Sequence   (paste as text only - not as screenshot or as 'code'):**
 * length of your protein in Amino Acids:** 507aa
 * Molecular Weight   of your protein in kiloDaltons using the [|Expasy ProtParam] website:** 58046.1 Da
 * Molar   Extinction coefficient    of your protein at 280 nm  wavelength  :**
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
 * CDS   Gene Sequence    (paste as text only):**
 * GC% Content for gene:** 6.5868263473054
 * CDS Gene Sequence (codon optimized) - copy from   output    of    Primer Design    Protocol (paste as text only):**
 * GC% Content for gene (codon optimized):**
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)

**
 * Primer design results for 'tail' primers (this is just 2 sequences): **