Calcium+Dependent+Protein+Kinase+4+(Plasmodium+Vivax)

Plasmodium vivax is a protozoal parasite causing the still wide-spread disease malaria. P. vivax is one of six species of malaria carriers that infect humans. Symptoms include fever, chills, headache, muscular aching and weakness, vomiting, cough, diarrhoea and abdominal pain. According to DNDi (Drugs for Neglected Diseases initiative) and other internet sources, malaria kills about one child every 30 seconds. This disease is common in hot and tropical regions of the world. __@http://www.tdrtargets.org/targets/view?gene_id=269414__
 * *Target (protein/gene name): ** Calcium Dependent Protein Kinase 4, putative
 * *NCBI Gene # or RefSeq#: ** 269414
 * *Protein ID (NP or XP #) or Wolbachia#: ** PVX_000555 (PlasmoDB)
 * *Organism (including strain): ** Plasmodium vivax
 * Etiologic Risk Group (see link below): **
 * */ Disease Information (sort of like the Intro to your Mini __Research Write__ up): **
 * Link to TDR Targets page (if present): **


 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) **

significant loss of fitness in bloodsstream forms (6 days) and no significant loss or gain of fitness in bloodstream from (3 days)
 * Essentiality of this protein: **


 * Gene/Ortholog: ** mtu1874 (OG4_10600); ** Phenotype: ** non-essential; ** Source study: ** nmpdr
 * Gene/Ortholog: ** mtu1142 (OG4_10600); ** Phenotype: ** essential; ** Source study: ** nmpdr
 * Gene/Ortholog: ** eco1968 (OG4_10600); ** Phenotype: ** undefined; ** Source study: ** blattner
 * Gene/Ortholog: ** eco1968 (OG4_10600); ** Phenotype: ** non-essential; ** Source study: ** gerdes
 * Gene/Ortholog: ** eco1968 (OG4_10600); ** Phenotype: ** non-essential; ** Source study: ** keio
 * Gene/Ortholog: ** eco1968 (OG4_10600); ** Phenotype: ** non-essential; ** Source study: ** shigen
 * Gene/Ortholog: ** cel16317 (OG4_10600); ** Phenotype: ** Larval/Adult Lethal/Arrest; ** Source study: ** neb
 * Gene/Ortholog: ** cel16317 (OG4_10600); ** Phenotype: ** Embryonic Lethal/Arrest; ** Source study: ** neb
 * Gene/Ortholog: ** cel16317 (OG4_10600); ** Phenotype: ** Embryonic Lethal/Arrest; ** Source study: ** wormbase
 * Gene/Ortholog: ** cel16317 (OG4_10600); ** Phenotype: ** Larval/Adult Lethal/Arrest; ** Source study: ** wormbase
 * Gene/Ortholog: ** Tb09.211.3180 (OG4_10600); ** Phenotype: ** significant loss of fitness in bloodstream forms (3 days); ** Source study: ** alsford
 * Gene/Ortholog: ** Tb09.211.3180 (OG4_10600); ** Phenotype: ** significant loss of fitness in bloodstream forms (6 days); ** Source study: ** alsford
 * Gene/Ortholog: ** Tb09.211.3180 (OG4_10600); ** Phenotype: ** significant loss of fitness in procyclic forms; ** Source study: ** alsford
 * Gene/Ortholog: ** Tb09.211.3180 (OG4_10600); ** Phenotype: ** significant loss of fitness in differentiation of procyclic to bloodstream forms; ** Source study: ** alsford

Is it a monomer or multimer as biological unit ** ? (make prediction at ** @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): Multimer


 * Complex of proteins?: ** No, but the active site may be in chain A or B
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): ** 0.8

2.7.1.37
 * *EC#: **

http://www.brenda-enzymes.org/enzyme.php?ecno=2.7.1.37 __ [] __. []®ion=US Glycylglycine: Quantity:132.12g Cost:$1,170.00 Supplier: PharmaGrade Catalog: G0674-100G
 * Link to BRENDA EC# page: **
 * -- ** Show screenshot of BRENDA enzyme mechanism schematic
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): ** continuous spectophotometric rate determination
 * -- link to Sigma (or other company ) page for assay (see Sigma links below) **
 * -- -or link (or citation) to paper that contains assay information **
 * -- links to assay reagents (substrates) pages. **
 * --- List cost and quantity of substrate reagents, supplier, and catalog # **

-- PDB # or closest PDB entry if using homology model: Homology model: PDB 2W8Z w/ lig -- For Homology Model option: Show pairwise alignment of your BLASTP search in NCBI against the PDB Query Coverage: Max % Identities: % Positives Chain used for homology:
 * Structure (PDB or Homology model) **

MRCNERNKKK AIFSNDDFSG EDTLMEDHLQ LREKLSEDIE MIKASLKNNL VCSTLNDNEI 60 LTLSNYMQFF VFKGGDLVIK QGEKGSYFFI INSGKFDVYV NDKKVKSMGK GSSFGEAALI 120 HNTQRSATIM AETDGTLWGV QRSTFRATLK QLSNRNFNEN RSFIDSVSVF DMLTEAQKNM 180 ITNACVIQMF KPGETIVKQG DYGDVLFILK EGKATVFIND KEIRVLNKGS YFGERALLYD 240 EPRSATIIAK EPTACASICR KLLNIVLGNL QVVLFRNIMT EALQQSEIFR QFSAEQLNDL 300 ADTAIVRDYP ANYHILHKDK VKSVKYLIVL EGKVELFLDD ESIGILTRGK SFGDQYVLNQ 360 KQKFRHTVKS LDVCKIALIT ESCLADCLGD NNIDASIDHN NKKSIIKKMY IFRYLSEQQC 420 NLLIEAFRTT RYEEGDYIIQ EGEVGSRFYI IKNGEVEVTK NGKRLRTLGK NDYFGERALL 480 YDEPRTASII SKATSVECWF VDKSVFLQII QGPMLTHLEE RIKMQDTKVE MHELETERII 540 GRGTFGTVKL VHHKPTQIRY ALKCVSKRSI ISLNQQNNIK LEREITAEND HPFIIRLVRT 600 FKDSNCFYFL TELVTGGELY DAIRKLGLLS KPQAQFYLGS IILAIEYLHE RNIVYRDLKP 660 ENILLDKQGY VKLIDFGCAK KIQGRAYTLV GTPHYMAPEV ILGKGYGCTV DIWALGVCLY 720 EFICGPLPFG NDQEDQLEIF RDILTGQLTF PDYVSDQDSI NLMKRLLCRL PQGRIGCSIN 780 GFKDIKEHAF FGNFNWDKLA GRLLEPPLVS KGETYAEDID IKQIEEEDAL NEGEPLDGDD 840 SWDVDF
 * Current Inhibitors: **
 * Expression Information (has it been expressed in bacterial cells): **
 * Purification Method : **
 * Image of protein (PyMol with features delineated and shown separately): **
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **
 * *length of your protein in Amino Acids: ** 846 aa
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website: ** 96548 kD
 * Molar Extinction coefficient of your protein at 280 nm wavelength: ** 66405 assuming all pairs of Cys residues form cystines


 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
 * [[image:vdsstream/TMPREDforPv6PG.JPG caption="TMPREDforPv6PG.JPG"]] ||
 * 2 strong possible transmembrane regions according to TMPred Expasy ||

TACTTCCAATCC ATG ** __ ACCGGCACTTGTGATATCGG __ ** TCTCATTGGTCTGGCGGTTATGGGTCAGAACCTGTCC CTGAACATCGCCTCTAACGGTTTTACCATCGGTGTTTACAACCGTACCTACGAGCGTACG GAAGATACCCTGAAGAAAGCGAAAGAGGGTAACCTGCCGATCCAAGGTTATGAAACCCTC GAGCAGCTGATCAACAACCTCAAGAAACCGCGTAAAATCATTCTGCTCATCAAAGCGGGT CCGGCGGTTGACGAAACCATTAAGAACATCCTGAAGCACTTTGAGGAAGGCGACATCATC ATCGACGGTGGTAACGAATGGTACCTCAATACCGAACGTCGTATCACTCTGTGCGAAGAG CACAAAGTAGAATACCTGGCGATGGGTGTTTCTGGTGGCGAAGCAGGTGCGCGCTATGGC TGTTCTTTCATGCCGGGTGGCTCCAAATATGCGTATGACACGATCAAAGACATCCTCGAG AAGTGCTCTGCGAAAGTTGGCACGAGCCCTTGCGTAACTTACATCGGTCCTCGTAGCTCT GGTAACTACGTTAAAATGGTTCACAACGGTATCGAATATGGCGATATGCAACTCATCTCC GAGTCTTACCTGCTGATGAAAAACATTCTGAACTACAATAACGAGAAACTGAGCGAAGTA TTCAAAAAATGGAACGAAGGTATCCTCAACTCTTATCTGATCGAAATCACCTATAAGATT CTGGGCAAGAAGGACGAACTCACGGACAACCATCTCGTTGACATGATCCTGGACATCGCG GGTGCTAAGGGTACGGGTAAATGGACCATGCTGGAAGCGATCGAGCGTGGTATCCCGTGC CCGACCATGTGTGCGGCGCTGGACGCCCGTAATATCTCCGCGTATAAACAGCTGCGCGTT AAGGCGGACTCTCATTTTTCTAACGACATGAAGGTTAACAAAGTTGAAGGTGAAAATCTC ATTAACTTTGAAGACGACCTCCTGAATGCGCTGTACTGCTGCAAGATCATTTCTTACACC CAGGGTCTGTTCCTGCTCAAACAGGTTTCTGAAGAGATGAAGTGGAATCTCAATCTGGGT GAAATCTCTCGTATTTGGCGTGGTGGCTGCATCATCCGCGCTGTCTTCCTGGATCGTATT ACTAACGCGTACAAGAAGAATGAAAAGCTCGACCTGCTCTTTCTCGACGAGGATTTCGCG CAGGAAATGAAGAACAAACTCCCGAGCCTGCGTAAAGTTGTTCAAGTTGCGACCAAATCT TCTATCCCGATCCCGGCGTTCAGCGCGTCTCTGGCCTACTTTCAGATGGTGACCTCTCAA AACCTCCCACTGAACCTGGTGCAGGCGCAACGTGACTACTTCGGCTCTCACACGTATAAG CGTGTTGACCGTGACGGT**__GACTTCCATACTATCTGGGAA__** TAA CAGTAAAGGTGGATA source: [] *took CDS gene sequence from primer design tails protocol and input into Oligo Analyzer with 0.4uM Oligo concentration, 50mM Na+ concentration, 2mM Mg concentration and 0.3mM dNTP concentration.
 * *CDS Gene Sequence (paste as text only): **
 * *GC% Content for gene: **
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **
 * *GC% Content for gene (codon optimized): ** 49.2%