Target-Mycobacterium+PSTP

Sadhana **ORGANISM:** //MYCOBACTERIUM TUBERCULOSIS H37Rv// **EC#** //3.1.3.16// **TARGET ENZYME**: //SERINE THREONINE PROTEIN PHOSPHATASE PSTP//

**NCBI GENE # OR REFSEQ #**//:887070//

**BACKGROUND INFORMATION:**
 Tuberculosis-infectious disease caused by Mycobacterium Tuberculosis- attacks the lungs  Can be acquired through inhalation  Pathogen of the mammalian respiratory system (infects lungs)  Neither gram-positive nor gram-negative (acid-fast staining used)  Cell wall highly concentrated with lipids-unique property  Divides slower than other bacteria  Taken up by alveolar macrophages-doesn’t get digested; thus multiplies  Symptoms: cough, fever, loss of appetite (infection can spread to other parts of the body)  PSTP phosphorylates proteins in mycobacterium tuberculosis, which help them sustain and multiply.

**IMAGE:**


>gi|57116681:c23181-21637 Mycobacterium tuberculosis H37Rv, complete genome GTGGCGCGCGTGACCCTGGTCCTGCGATACGCGGCGCGCAGCGATCGCGGCTTGGTACGCGCCAACAACGAAGACTCGG TCTACGCTGGGGCACGGCTATTGGCCCTGGCCGACGGCATGGGTGGGCATGCGGCCGGCGAGGTGGCGTCCCAGTTGG TGATTGCCGCATTGGCCCATCTCGATGACGACGAGCCCGGTGGCGATCTGCTGGCCAAGCTGGATGCCGCGGTGCGCGC CGGCAACTCGGCTATCGCAGCGCAAGTCGAGATGGAGCCCGATCTCGAAGGCATGGGTACCACGCTCACCGCAATCCTGT TCGCGGGCAACCGGCTCGGCCTGGTGCATATCGGTGACTCGCGCGGTTACCTGCTGCGCGACGGTGAGCTGACGCAGAT CACCAAGGACGACACGTTTGTCCAAACGCTGGTCGACGAAGGCCGGATCACCCCGGAGGAGGCGCACAGCCACCCGCAAC GCTCGTTGATCATGCGGGCGTTGACCGGCCATGAGGTCGAACCGACGCTGACCATGCGAGAAGCCCGCGCCGGTGATCG TTACCTGCTGTGCTCGGACGGGTTGTCCGATCCGGTTAGCGATGAAACTATCCTCGAGGCCCTGCAGATCCCCGAGGTTG CCGAGAGCGCTCACCGCCTCATTGAACTGGCGCTGCGCGGCGGCGGCCCCGACAACGTCACTGTCGTCGTCGCCGACGT CGTCGACTACGACTACGGCCAGACCCAACCGATTCTGGCCGGGGCGGTCTCAGGCGACGACGACCAACTGACCCTGCCCA <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">ACACCGCCGCCGGCCGGGCCTCTGCCATCAGCCAGCGCAAGGAGATCGTTAAACGCGTTCCGCCACAGGCCGATACATTC <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">AGTCGGCCACGGTGGTCGGGCCGACGGCTAGCATTCGTTGTCGCACTGGTGACCGTGCTGATGACTGCGGGCCTGCTCA <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">TTGGTCGCGCGATCATCCGCAGCAACTACTACGTAGCGGACTACGCCGGCAGCGTGTCCATCATGCGGGGGATTCAAGGG <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">TCGCTACTGGGCATGTCCCTGCACCAGCCTTACCTGATGGGCTGCCTCAGCCCGCGTAACGAGCTGTCGCAGATCAGCTAC <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">GGACAGTCTGGGGGCCCTCTCGACTGCCATCTGATGAAACTGGAGGATCTGCGACCGCCGGAGCGCGCACAGGTTCGGGC <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">CGGTCTCCCGGCCGGCACTCTCGATGACGCCATCGGGCAGTTGCGCGAACTGGCGGCCAACTCCCTGCTGCCGCCTTGCC <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">CGGCGCCGCGTGCCACGTCCCCGCCCGGGCGCCCGGCCCCACCCACCACCAGCGAGACAACCGAACCAAACGTCACCTCC <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">TCGCCAGCCTCTCCATCACCCACCACCTCCGCGCCGGCCCCCACCGGAACTACTCCTGCCATCCCCACGAGTGCCTCCCCG <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">GCAGCGCCCGCGTCGCCGCCGACGCCTTGGCCCGTCACCAGCTCGCCGACGATGGCCGCACTTCCGCCACCCCCGCCTCA <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">GCCGGGCATCGACTGCCGGGCGGCGGCATGA
 * <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">CDS GENE SEQUENCE: **

<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">>1TXO:A|PDBID|CHAIN|SEQUENCE
 * <span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">AMINO ACID SEQUENCE: **

<span style="background-color: #ffff00; color: #000000; font-family: Arial; font-size: 16px; text-decoration: none; vertical-align: baseline;">MARVTLVLRYAARSDRGLVRANNEDSVYAGARLLALADGMGGHAAGEVASQLVIAALAHLDDDEPGGDLLAKLDAA <span style="background-color: #ffff00; color: #000000; font-family: Arial; font-size: 16px; text-decoration: none; vertical-align: baseline;">VRAGNSAIAAQVEMEPDLEGMGTTLTAILFAGNRLGLVHIGDSRGYLLRDGELTQITKDDTFVQTLVDEGRITPEEAHSH <span style="background-color: #ffff00; color: #000000; font-family: Arial; font-size: 16px; text-decoration: none; vertical-align: baseline;">PQRSLIMRALTGHEVEPTLTMREARAGDRYLLCSDGLSDPVSDETILEALQIPEVAESAHRLIELALRGGGPDNVTVVVADVVD

=serine/threonine phosphatase [Mycobacterium tuberculosis H37Rv]= NCBI Reference Sequence: NP_214532.1 [|GenPept] [|Graphics] >gi|15607160|ref|NP_214532.1| serine/threonine phosphatase [Mycobacterium tuberculosis H37Rv] <span class="ff_line">MARVTLVLRYAARSDRGLVRANNEDSVYAGARLLALADGMGGHAAGEVASQLVIAALAHLDDDEPGGDLL AKLDAAVRAGNSAIAAQVEMEPDLEGMGTTLTAILFAGNRLGLVHIGDSRGYLLRDGELTQITKDDTFVQ TLVDEGRITPEEAHSHPQRSLIMRALTGHEVEPTLTMREARAGDRYLLCSDGLSDPVSDETILEALQIPE VAESAHRLIELALRGGGPDNVTVVVADVVDYDYGQTQPILAGAVSGDDDQLTLPNTAAGRASAISQRKEI VKRVPPQADTFSRPRWSGRRLAFVVALVTVLMTAGLLIGRAIIRSNYYVADYAGSVSIMRGIQGSLLGMS LHQPYLMGCLSPRNELSQISYGQSGGPLDCHLMKLEDLRPPERAQVRAGLPAGTLDDAIGQLRELAANSL LPPCPAPRATSPPGRPAPPTTSETTEPNVTSSPASPSPTTSAPAPTGTTPAIPTSASPAAPASPPTPWPV TSSPTMAALPPPPPQPGIDCRAAA

<span class="ff_line">Molecular Weight - Dr. B 53811.9

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient- Dr. B 27640

Filtered DNA Sequence: ATGGCACGCGTTACCCTCGTTCTGCGTTACGCTGCGCGTTCTGACCGTGGTCTGGTTCGC GCAAACAATGAGGACTCTGTTTACGCGGGTGCTCGTCTCCTCGCGCTGGCCGACGGCATG GGTGGTCACGCTGCCGGTGAAGTTGCGTCTCAGCTGGTTATCGCAGCCCTGGCGCATCTC GACGATGACGAACCGGGTGGTGACCTCCTGGCGAAACTGGACGCCGCAGTGCGTGCCGGT AACTCTGCGATTGCGGCGCAGGTTGAAATGGAACCGGACCTGGAGGGTATGGGTACCACC CTGACGGCGATCCTGTTCGCGGGTAATCGTCTGGGTCTCGTTCACATCGGTGACTCTCGT GGTTACCTGCTGCGTGACGGTGAACTGACTCAGATCACCAAAGACGACACCTTTGTTCAG ACTCTGGTTGACGAAGGTCGTATCACCCCGGAAGAAGCGCACTCTCATCCGCAACGTTCT CTGATCATGCGCGCTCTCACGGGCCACGAAGTTGAACCAACCCTCACCATGCGTGAAGCG CGTGCGGGTGACCGCTACCTCCTCTGCTCTGATGGTCTCTCTGACCCTGTTTCTGACGAA ACCATCCTCGAAGCGCTGCAGATTCCGGAGGTTGCTGAATCCGCCCATCGCCTGATTGAA CTGGCGCTCCGTGGTGGCGGTCCGGATAATGTTACTGTTGTAGTTGCCGATGTCGTTGAC TAA

**PDB STRUCTURE IDENTIFIER:** //2CM1//
//Molecular Weight of protein: 60335.4//

Forward Primer: TACTTCCAATCC__ATGGCACGCGTTACCCTCGT__

32 bp GC Content: 53.1% 0mM Mg: 67.1C 1.5mM Mg: 73.8C 2mM Mg: 74.3C 4mM Mg: 75.1C 6mM Mg: 75.5C

Reverse Primer: TATCCACCTTTACTG__TTAGTCAACGACATCGGCAA__

35 bp GC Content: 42.9% 0mM Mg: 63.3C 1.5mM Mg: 70.8C 2mM Mg: 71.3C 4mM Mg: 72.2C 6mM Mg: 72.7C

__ VirtualPlasmid __ TAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTCCAAT__CC__ __ ATG ____ GCACGCGTTACCCTCGTTCTGCGTTACGCTGCGCGTTCTGACCGTGGTCTGGTTCGCGCAAACAATGAGGACTCTGTTTACGCGGGTGCTCGTCTCCTCGCGCTGGCCGACGGCATGGGTGGTCACGCTGCCGGTGAAGTTGCGTCTCAGCTGGTTATCGCAGCCCTGGCGCATCTCGACGATGACGAACCGGGTGGTGACCTCCTGGCGAAACTGGACGCCGCAGTGCGTGCCGGTAACTCTGCGATTGCGGCGCAGGTTGAAATGGAACCGGACCTGGAGGGTATGGGTACCACCCTGACGGCGATCCTGTTCGCGGGTAATCGTCTGGGTCTCGTTCACATCGGTGACTCTCGTGGTTACCTGCTGCGTGACGGTGAACTGACTCAGATCACCAAAGACGACACCTTTGTTCAGACTCTGGTTGACGAAGGTCGTATCACCCCGGAAGAAGCGCACTCTCATCCGCAACGTTCTCTGATCATGCGCGCTCTCACGGGCCACGAAGTTGAACCAACCCTCACCATGCGTGAAGCGCGTGCGGGTGACCGCTACCTCCTCTGCTCTGATGGTCTCTCTGACCCTGTTTCTGACGAAACCATCCTCGAAGCGCTGCAGATTCCGGAGGTTGCTGAATCCGCCCATCGCCTGATTGAACTGGCGCTCCGTGGTGGCGGTCCGGATAATGTTACTGTTGTAGTTGCCGATGTCGTTGACTAA ____ C __ AGTAAAGGTGGATACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGATTGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAAT