protein-L-histidine+N-phosphotransferase

EspA
 * *Target (protein/gene name): **

885377
 * *NCBI Gene # or RefSeq#: **

NP_218133.1
 * *Protein ID (NP or XP #) or Wolbachia#: **

//Mycobacterium tuberculosis//
 * *Organism (including strain): **

Tuberculosis is commonly known as a respiratory infection caused by //Mycobacterium tuberculosis// and can become fatal if not treated. It can be spread through the air by people who are already infected. The symptoms of tuberculosis include chest pains, coughing up blood or phlegm, and a bad cough that can last 3 weeks or longer.
 * Etiologic Risk Group (see link below): **
 * */ Disease Information (sort of like the Intro to your Mini Research Write up): **

N/A
 * Link to TDR Targets page (if present): **


 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) **

[]

This protein is essential to the ESX-1 secretion apparatus, which provides the principal virulence factors ESAT-6 and CFP-10 Is it a monomer or multimer as biological unit ** ? (make prediction at ** @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): No
 * Essentiality of this protein: **
 * Complex of proteins?: **


 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **

[]


 * *EC#: **

2.7.13.3


 * Link to BRENDA EC# page: **

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 * ATP Protein + L-histidine = ADP + Protein N(tau)-phospho-L-histidine**

Supernatant fluid was filtered and then run through an enzyme-linked immunosorbent assay (ELISA)
 * -- ** Show screenshot of BRENDA enzyme mechanism schematic
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **


 * -- link to Sigma (or other company ) page for assay (see Sigma links below) **
 * -- -or link (or citation) to paper that contains assay information **

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 * -- links to assay reagents (substrates) pages. **
 * --- List cost and quantity of substrate reagents, supplier, and catalog # **

-- PDB # or closest PDB entry if using homology model: -- For Homology Model option: Show pairwise alignment of your BLASTP search in NCBI against the PDB Query Coverage: Max % Identities: % Positives Chain used for homology:
 * Structure (PDB or Homology model) **

PDB #: P9WJE0 (no PDB entries available for this one or homologs)

Purified with the use of nickel agarose
 * Current Inhibitors: **
 * Expression Information (has it been expressed in bacterial cells): **
 * Purification Method : **
 * Image of protein (PyMol with features delineated and shown separately): **




 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **

code format="sequence" 10        20         30         40         50 MSRAFIIDPT ISAIDGLYDL LGIGIPNQGG ILYSSLEYFE KALEELAAAF 60        70         80         90        100 PGDGWLGSAA DKYAGKNRNH VNFFQELADL DRQLISLIHD QANAVQTTRD 110       120        130        140        150 ILEGAKKGLE FVRPVAVDLT YIPVVGHALS AAFQAPFCAG AMAVVGGALA 160       170        180        190        200 YLVVKTLINA TQLLKLLAKL AELVAAAIAD IISDVADIIK GTLGEVWEFI 210       220        230        240        250 TNALNGLKEL WDKLTGWVTG LFSRGWSNLE SFFAGVPGLT GATSGLSQVT 260       270        280        290        300 GLFGAAGLSA SSGLAHADSL ASSASLPALA GIGGGSGFGG LPSLAQVHAA 310       320        330        340        350 STRQALRPRA DGPVGAAAEQ VGGQSQLVSA QGSQGMGGPV GMGGMHPSSG 360       370        380        390 ASKGTTTKKY SEGAAAGTED AERAPVEADA GGGQKVLVRN VV code

392
 * *length of your protein in Amino Acids **

39.89 kDa
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website **

0.951 L/mol cm
 * Molar Extinction coefficient of your protein at 280 nm wavelength: **


 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.



code ATGAGCAGAGCGTTCATCATCGATCCAACGATCAGTGCCATTGACGGCTTGTACGACCTTCTGGGGATTG GAATACCCAACCAAGGGGGTATCCTTTACTCCTCACTAGAGTACTTCGAAAAAGCCCTGGAGGAGCTGGC AGCAGCGTTTCCGGGTGATGGCTGGTTAGGTTCGGCCGCGGACAAATACGCCGGCAAAAACCGCAACCAC GTGAATTTTTTCCAGGAACTGGCAGACCTCGATCGTCAGCTCATCAGCCTGATCCACGACCAGGCCAACG CGGTCCAGACGACCCGCGACATCCTGGAGGGCGCCAAGAAAGGTCTCGAGTTCGTGCGCCCGGTGGCTGT GGACCTGACCTACATCCCGGTCGTCGGGCACGCCCTATCGGCCGCCTTCCAGGCGCCGTTTTGCGCGGGC GCGATGGCCGTAGTGGGCGGCGCGCTTGCCTACTTGGTCGTGAAAACGCTGATCAACGCGACTCAACTCC TCAAATTGCTTGCCAAATTGGCGGAGTTGGTCGCGGCCGCCATTGCGGACATCATTTCGGATGTGGCGGA CATCATCAAGGGCACCCTCGGAGAAGTGTGGGAGTTCATCACAAACGCGCTCAACGGCCTGAAAGAGCTT TGGGACAAGCTCACGGGGTGGGTGACCGGACTGTTCTCTCGAGGGTGGTCGAACCTGGAGTCCTTCTTTG CGGGCGTCCCCGGCTTGACCGGCGCGACCAGCGGCTTGTCGCAAGTGACTGGCTTGTTCGGTGCGGCCGG TCTGTCCGCATCGTCGGGCTTGGCTCACGCGGATAGCCTGGCGAGCTCAGCCAGCTTGCCCGCCCTGGCC GGCATTGGGGGCGGGTCCGGTTTTGGGGGCTTGCCGAGCCTGGCTCAGGTCCATGCCGCCTCAACTCGGC AGGCGCTACGGCCCCGAGCTGATGGCCCGGTCGGCGCCGCTGCCGAGCAGGTCGGCGGGCAGTCGCAGCT GGTCTCCGCGCAGGGTTCCCAAGGTATGGGCGGACCCGTAGGCATGGGCGGCATGCACCCCTCTTCGGGG GCGTCGAAAGGGACGACGACGAAGAAGTACTCGGAAGGCGCGGCGGCGGGCACTGAAGACGCCGAGCGCG CGCCAGTCGAAGCTGACGCGGGCGGTGGGCAAAAGGTGCTGGTACGAAACGTCGTCTAA code 63.4%
 * *CDS Gene Sequence (paste as text only): **
 * *GC% Content for gene: **


 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **
 * *GC% Content for gene (codon optimized): **

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)

**
 * Primer design results for 'tail' primers (this is just 2 sequences): **