Salmonella+enterica+subsp.+enterica+serovar+Typhimurium

Do Not Need this info for Spring (but still copy these lines to your Target page for now) **Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):** **( link to DNA Works output text file - **that should be saved in your Google Docs folder after you did the primer design protocol) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Target (protein/gene name):**
 * NCBI Gene # or RefSeq#:**
 * Protein ID (NP or XP #) or Wolbachia#:**
 * Organism (including strain):**
 * Etiologic Risk Group (see link below):**
 * Background/Disease Information (sort of like the Intro to your Mini Research Write up):** **Essentiality of this protein:** **Complex of proteins?:** **Druggable Target:**
 * *EC#: ** **Link to BRENDA EC# page:** **--** Show screenshot of BRENDA enzyme mechanism schematic
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):** **-- link to Sigma (or other company) page for assay or assay reagents (substrates)** **-- link (or citation) to paper that contains assay information** **-- List cost and quantity of substrate reagents and supplier** **Structure Available (PDB or Homology model)** -- PDB # or closest PDB entry if using homology model: -- For Homology Model option: Show pairwise alignment of your BLASTP search in NCBI against the PDB  Query Coverage:  Max % Identities:  % Positives  Chain used for homology:
 * Current Inhibitors:** **Expression Information (has it been expressed in bacterial cells):** **Purification Method:** **Image of protein (PyMol with features delineated and shown separately):**
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):**
 * length of your protein in Amino Acids** **Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website**
 * Molar Extinction coefficient of your protein at 280 nm wavelength:** ** TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
 * CDS Gene Sequence (paste as text only):**
 * GC% Content for gene:**
 * CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):** ***GC% Content for gene (codon optimized):**
 * Primer design results for 'tail' primers (this is just 2 sequences):**

Resources:
See **ProtocolTargetDiscoveryVDS.docx** for more Etiologic Risk Group Categories (for pathogens): http://www.utexas.edu/research/rsc/ibc/agent_class.html#_Toc7238334
 * Databases of genes/organisms:** @http://www.niaid.nih.gov/Pages/default.aspx @http://eupathdb.org/eupathdb/ @https://patricbrc.vbi.vt.edu/portal/portal/patric/Home @http://www.nmpdr.org/FIG/wiki/view.cgi/Main/EssentialGenes @http://tubic.tju.edu.cn/deg/ @http://csgid.org/csgid/cake/pages/community_request_gateway @http://tdrtargets.org/ @http://gsc.jcvi.org/status.shtml
 * Scientific Nomenclature page from Center for Disease Control (gene, protein names and abbreviations)** @http://wwwnc.cdc.gov/eid/pages/scientific-nomenclature.htm
 * Gene Information:** NCBI GENE Page: @http://www.ncbi.nlm.nih.gov/gene BLAST Page: @http://blast.ncbi.nlm.nih.gov/
 * Protein Information:** NCBI Protein Page: @http://www.ncbi.nlm.nih.gov/protein Protein Expression Website Protein Expression Paper: [[file:vdsstream/SGC_ProteinProductionPurificationNatMethods2008.pdf|SGC_ProteinProductionPurificationNatMethods2008.pdf]]
 * Primer Overlap PCR Articles** [[file:vdsstream/HooverLubkowski_PCRoverlapcloninggnf042.pdf|HooverLubkowski_PCRoverlapcloninggnf042.pdf]] [[file:vdsstream/StemmerPCRoverlapGene1995.pdf|StemmerPCRoverlapGene1995.pdf]]
 * Is my target good for Virtual Screening programs?** [[file:vdsstream/Reynolds_THermodynamicsLigandBinding_MedChemLett2011.pdf|Reynolds_THermodynamicsLigandBinding_MedChemLett2011.pdf]]