TargetSummer17+-+Serine+Threonine+Protein+Phosphatase+5

** Target (protein/gene name): S **erine/Threonine Protein Phosphatase 5
@http://www.tdrtargets.org/targets/view?gene_id=19618 Tb927.10.13670 has essentiality data Gene/Ortholog: Tb927.10.13670 (OG4_11778); Phenotype: no significant loss or gain of fitness in bloodstream forms (3 days); Source study: alsford Gene/Ortholog: Tb927.10.13670 (OG4_11778); Phenotype: significant loss of fitness in bloodstream forms (6 days); Source study: alsford Gene/Ortholog: Tb927.10.13670 (OG4_11778); Phenotype: significant loss of fitness in procyclic forms; Source study: alsford Gene/Ortholog: Tb927.10.13670 (OG4_11778); Phenotype: significant loss of fitness in differentiation of procyclic to bloodstream forms; Source study: alsford Is it a monomer or multimer as biological unit ** ? (make prediction at ** @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html):
 * NCBI Gene # or RefSeq#: **
 * Protein ID (NP or XP #) or Wolbachia#: **
 * Organism (including strain): **// T. brucei //
 * Etiologic Risk Group (see link below): **
 * */ Disease Information (sort of like the Intro to your Mini Research Write up): **
 * Link to TDR Targets page (if present): **
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) **
 * Essentiality of this protein: **
 * Complex of proteins?: **
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **


 * *EC#: ** 3.1.3.16
 * Link to BRENDA EC# page: **
 * -- ** Show screenshot of BRENDA enzyme mechanism schematic
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **

Assay: Assay for Calcineurin (3.1.3.16 ); Source: Sigma-Aldrich Description: Automatic link to known assays based on EC numbers. Assay: Assayed; Source: BRENDA Description: An enzyme with this EC number or name or sequence has been assayed in Trypanosoma brucei ( 1 );
 * -- link to Sigma (or other company ) page for assay (see Sigma links below) **
 * -- -or link (or citation) to paper that contains assay information **
 * -- links to assay reagents (substrates) pages. **
 * --- List cost and quantity of substrate reagents, supplier, and catalog # **

-- PDB # or closest PDB entry if using homology model: -- For Homology Model option: Show pairwise alignment of your BLASTP search in NCBI against the PDB Query Coverage: Max % Identities: % Positives Chain used for homology:
 * Structure (PDB or Homology model) **


 * Current Inhibitors: **
 * Expression Information (has it been expressed in bacterial cells): **
 * Purification Method : **
 * Image of protein (PyMol with features delineated and shown separately): **
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **
 * *length of your protein in Amino Acids **
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website **
 * Molar Extinction coefficient of your protein at 280 nm wavelength: **
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
 * *CDS Gene Sequence (paste as text only): **
 * *GC% Content for gene: **
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **
 * *GC% Content for gene (codon optimized): **

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)

**
 * Primer design results for 'tail' primers (this is just 2 sequences): **