TargetSp15-BETA-KETOACYL-ACP+SYNTHASE+III+(p.+Falciparum)

Format for Individual Target pages (copy this list to new Target page and then fill in for your target):
*Target (protein/gene name): BETA-KETOACYL-ACP SYNTHASE I [|__http://www.tdrtargets.org/targets/view?gene_id=4612__]

*NCBI Gene # or RefSeq#: 3885996 [|__http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=full_report&list_uids=3885996__]

*Protein ID (NP or XP #) or Wolbachia#: XP_966246.2

[|__http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=full_report&list_uids=3885996__]

*Organism (including strain): = Plasmodium falciparum 3D7 = Etiologic Risk Group (see link below): Group B disease [|__http://ideas.health.vic.gov.au/bluebook/malaria.asp__] http://www.tulane.edu/~wiser/malaria/fv.html

*/Disease Information (sort of like the Intro to your Mini __Research Write__ up): Malaria is one of the most predominate diseases in the world. Around 3.5 million people are have a moderate to high potential of getting the disease. It mainly is in Africa, India, and in central South America because of the presence of the // Anopheles gambiae // mosquito that serves as a carrier to disease causing //Plasmodium flaciparum//. One way the // Plasmodium flaciparum //produces proteins is by using the amino acids of the hemoglobin on erthyrocytes. This causes the oxygen transport that takes place through the blood cells to be hindered. Symptoms of malaria are nausea, vomit, diarrhia, and anemia.

Link to TDR Targets page (if present): [|__http://www.tdrtargets.org/targets/view?gene_id=4612__] Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) [|__http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=full_report&list_uids=3885996__] Essentiality of this protein: Catalyzes the biosynthesis of fatty acids. http://www.rcsb.org/pdb/explore/explore.do?structureId=1HN9

Is it a monomer or multimer as biological unit? (make prediction at [|__http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html__] ): 2 amino acid chains and 2 ligands in ASU Complex of proteins?: Yes Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): 0.5 [|__http://www.tdrtargets.org/targets/view?gene_id=4612__] Structure-activity relationships at the 5-position of thiolactomycin: an intact (5R)-isoprene unit is required for activity against the condensing enzymes from Mycobacterium tuberculosis and Escherichia coli. (2006). J Med Chem 49: 159-71.

*EC#: 2.3.1.41 Link to BRENDA EC# page: [|__http://www.brenda-enzymes.org/enzyme.php?ecno=2.3.1.41&Suchword=&organism%5B%5D=Plasmodium+falciparum&show_tm=0__] -- Show screenshot of BRENDA enzyme mechanism schematic == ==  Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): [|__http://www.ncbi.nlm.nih.gov/pubmed/18437316__] -- link to Sigma (or other company) page for assay (see Sigma links below) -- -or link (or citation) to paper that contains assay information -- links to assay reagents (substrates) pages. [|__http://download-v2.springer.com/static/pdf/329/chp%253A10.1007%252F978-1-59745-246-5_16.pdf?token2=exp=1429408833~acl=%2Fstatic%2Fpdf%2F329%2Fchp%25253A10.1007%25252F978-1-59745-246-5_16.pdf*~hmac=c4d17d97c2d4acc7414488569db5814860f50aefe7afdc1a5f0e7ab04e9f40d9__] --- List cost and quantity of substrate reagents, supplier, and catalog # Substrate reagent: Lauroyl CoA Quantity: 5MG, Catolog #: L2659, Cost: $154.00, Supplier: Sigma

Structure (PDB or Homology model) -- PDB # or closest PDB entry if using homology model: 1HN9

Current Inhibitors: http://www.drugbank.ca/biodb/bio_entities/BE0000801
 * Cerulenin **

Expression Information (has it been expressed in bacterial cells): E.coli Purification Method: Expressed in E. coli with an N-terminal polyhistidine tag and subsequently purified by metal chelate and size exclusion chromatography. [|__http://aac.asm.org/content/46/5/1310__]

Image of protein (PyMol with features delineated and shown separately): *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHIAAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVAT

TSATHAFPSAACQIQSMLGIKGCPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG

AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVTELAHIVDETLAANNLDRSQLDW

LVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTSAASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF

*length of your protein in Amino Acids 317 residues Molecular Weight of your protein in kiloDaltons using the [|__Expasy ProtParam__] website 69441.6 Molar Extinction coefficient of your protein at 280 nm wavelength: 626262 TMpred graph Image ( [|__http://www.ch.embnet.org/software/TMPRED_form.html__] ). Input your amino acid sequence to it. [|__http://www.ch.embnet.org/cgi-bin/TMPRED_form_parser__]
 * [[image:https://lh5.googleusercontent.com/kt8QqeGmsSuR4KNeVTRR8qmRhhvEw_bZydzqLNJr2QhFhZOdWPpqDHsLTjXqmfvOy77Eu9MoPVu_gQzHrkSxgAQsOCdvsvO8wE1nylI94GrQ2KumELt77TWTfgt043NqX7zpPJw align="center" caption="results" link="http://www.ch.embnet.org/cgi-bin/TMPRED_form_parser"]] ||
 * results ||

*CDS Gene Sequence (paste as text only):

atgtataccaaaattattggcaccggcagctatctgccggaacaggtgcgcaccaacgcg gatctggaaaaaatggtggataccagcgatgaatggattgtgacccgcaccggcattcgc gaacgccatattgcggcgccgaacgaaaccgtgagcaccatgggctttgaagcggcgacc cgcgcgattgaaatggcgggcattgaaaaagatcagattggcctgattgtggtggcgacc accagcgcgacccatgcgtttccgagcgcggcgtgccagattcagagcatgctgggcatt aaaggctgcccggcgtttgatgtggcggcggcgtgcgcgggctttacctatgcgctgagc gtggcggatcagtatgtgaaaagcggcgcggtgaaatatgcgctggtggtgggcagcgat gtgctggcgcgcacctgcgatccgaccgatcgcggcaccattattatttttggcgatggc gcgggcgcggcggtgctggcggcgagcgaagaaccgggcattattagcacccatctgcat gcggatggcagctatggcgaactgctgaccctgccgaacgcggatcgcgtgaacccggaa aacagcattcatctgaccatggcgggcaacgaagtgtttaaagtggcggtgaccgaactg gcgcatattgtggatgaaaccctggcggcgaacaacctggatcgcagccagctggattgg ctggtgccgcatcaggcgaacctgcgcattattagcgcgaccgcgaaaaaactgggcatg agcatggataacgtggtggtgaccctggatcgccatggcaacaccagcgcggcgagcgtg ccgtgcgcgctggatgaagcggtgcgcgatggccgcattaaaccgggccagctggtgctg ctggaagcgtttggcggcggctttacctggggcagcgcgctggtgcgcttt *GC% Content for gene: 60.3% *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): TACCAACGCTGACCTGGAAAAAATGGTTGACACCTCTGACGAATGGATCG 100 TTACCCGTACCGGTATCCGTGAACGTCACATCGCTGCTCCGAACGAAACC 150 GTTTCTACCATGGGTTTCGAAGCTGCTACCCGTGCTATCGAAATGGCTGG 200 TATCGAAAAAGACCAGATCGGTCTGATCGTTGTTGCTACCACCTCTGCTA 250 CCCACGCTTTCCCGTCTGCTGCTTGCCAGATCCAGTCTATGCTGGGTATC 300 AAAGGTTGCCCGGCTTTCGACGTTGCTGCTGCTTGCGCTGGTTTCACCTA 350 CGCTCTGTCTGTTGCTGACCAGTACGTTAAATCTGGTGCTGTTAAATACG 400 CTCTGGTTGTTGGTTCTGACGTTCTGGCTCGTACCTGCGACCCGACCGAC 450 CGTGGTACCATCATCATCTTCGGTGACGGTGCTGGTGCTGCTGTTCTGGC 500 TGCTTCTGAAGAACCGGGTATCATCTCTACCCACCTGCACGCTGACGGTT 550 CTTACGGTGAACTGCTGACCCTGCCGAACGCTGACCGTGTTAACCCGGAA 600 AACTCTATCCACCTGACCATGGCTGGTAACGAAGTTTTCAAAGTTGCTGT 650 TACCGAACTGGCTCACATCGTTGACGAAACCCTGGCTGCTAACAACCTGG 700 ACCGTTCTCAGCTGGACTGGCTGGTTCCGCACCAGGCTAACCTGCGTATC 750 ATCTCTGCTACCGCTAAAAAACTGGGTATGTCTATGGACAACGTTGTTGT 800 TACCCTGGACCGTCACGGTAACACCTCTGCTGCTTCTGTTCCGTGCGCTC 850 TGGACGAAGCTGTTCGTGACGGTCGTATCAAACCGGGTCAGCTGGTTCTG 900 CTGGAAGCTTTCGGTGGTGGTTTCACCTGGGGTTCTGCTCTGGTTCGTTT 950 C ||
 * ATGTACACCAAAATCATCGGTACCGGTTCTTACCTGCCGGAACAGGTTCG 50

*GC% Content for gene (codon optimized): 53.3%