TbPIP5K1+(phosphatidylinositol+4-phosphate+5-kinase)+Trypanosoma+brucei

//**Target:**// TbPIP5K1 (phosphatidylinositol 4-phosphate 5-kinase) //**NCBI Gene # or RefSeq#: **// NCBI 185431 //**Protein ID (NP or XP #) or Wolbachia#: **// XP_844312.1. //**Organism (including strain): **// //Trypanosoma brucei// (strain 427) //**Etiologic Risk Group: **// BioSafety Level 2 //**Disease Information**//: //Trypanosoma brucei// is a parasitic protozoan that causes Human African Trypanosomiasis (HAT), commonly known as African sleeping sickness. Trypanosomiasis is primarily transmitted by the tsetse fly, and is thus endemic to sub-Saharan Africa. Trypanosomiasis symptoms include fever, headache, and adenopathy in the first stage, during which //T. brucei// invades the circulatory and lymphatic systems. This stage lasts approximately one to two weeks. If trypanosomiasis is left untreated in the first stage, the disease can be fatal in the second stage. During this stage, //T. brucei// invades the central nervous system and causes various neuropsychiatric symptoms, including tremors and eventually coma. The entire disease progression takes only approximately three weeks to manifest, ending in fatality.

//**Link to TDR Targets page:**// [] //**Link to Gene Database page: **// [] //**Essentiality of this protein: **// Essential in //T. brucei//

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Is it a monomer or multimer as biological unit? (make prediction at @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Complex of proteins?: <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):

//**<span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">EC#: **// <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">2.7.1.68 //**<span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Link to BRENDA EC# page: **// []
 * <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Figure 1: **Reaction mechanism catalyzed by TbPIP5K1

//**<span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Enzyme Assay information: **// <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Adenosine 5'-triphosphate Bioluminescence Assay Kit
 * <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif; line-height: 1.5;">Cost - $299.50
 * <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif; line-height: 1.5;">Substrate reagents: dilution buffer, ATP standard, assay mix
 * <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif; line-height: 1.5;">no catalog #
 * []

<span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">//**PDB # or closest PDB entry if using homology model****:**// 2YBX //**<span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Current Inhibitors: **// <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">unknown //**<span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Expression Information: **// <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Yes, TbPIP5K1 has been successfully expressed in //E. coli//. //**<span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Purification Method: **// <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Nickel resin tubes //**<span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Image of protein: **// <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">

//**<span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Amino Acid Sequence: **// code <span style="background-color: #ffffff; font-family: monospace,monospace; font-size: 12px;">MPFNREILAAISFESGPLSSAEIAARRTPTEEPPKNLEVKSCDKRDEPRQNEATINLIRC MKWALGQSITSISHPAKQRPLNPKRDFSIKTKINLDVGVEKPKPGFCCSTAAATGVREQR NHHAPFYDAHGDSEEASHLDESDVESHDEVPNVPLAFVDYSPMCYRHIREFFNIDHRAYR DVLLRSRWHSTPTPGKSAAQLFFCGRDWVIKTMTHEESKFLRRILHRYYFFVLDHPYTLL PHFVGHHRLEINGEKMYIIIMQNVFVTGNTIHEKYDLKGSTVGRFATEAEKKKQTCTQKD LDINRPLHVGATRRNLLIEQMKMDCGFLNQCNIMDYSFLVGIHVLPMYEAHRPAPSTGSR EYLGGAAALATSALEPSYLLGMGETMQPMALNGHPTAAADSAPTDGRCFKADQGGMMSAD HPGGRREIYYIGIIDILQEYNSRKSMETLVFGMLKDRQKISAVPPKEYAARFVSFVSSII V code

<span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">//**Length**//**:** 481 amino acids <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Molar Extinction coefficient of your protein at 280 nm wavelength: //**<span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">TMpred graph Image: **// <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif; line-height: 0px; overflow: hidden;"> //**<span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">CDS Gene Sequence: **// <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">*GC% Content for gene: <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">*CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">*GC% Content for gene (codon optimized):
 * //<span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Molecular Weight: //** <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">54.379 kDa

<span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Do Not Need this info for Spring (but still copy these lines to your Target page for now) <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">(link to DNA Works output text file - that should be saved in your Google Docs folder after you did the primer design protocol) <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.

<span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">Primer design results for 'tail' primers (this is just 2 sequences): <span style="font-family: 'Palatino Linotype','Book Antiqua',Palatino,serif;">**