Probable+cation-transporter+ATPase+I+CtpI,+Mycobacterium+tuberculosis


 * Target (protein/gene name): **Probable cation-transporter ATPase I CtpI
 * NCBI Gene # or RefSeq #: **NC_000962.3
 * PBD ID: **3MD0
 * Organism: **//Mycobacterium //tuberculosis
 * Etiologic Risk Group: **Group 3
 * 1) [|http://www.bacterio.net/-hazard.html#group2]


 * Disease Information: **Tuberculosis is a bacterial disease that is spread through the air from person to person. It is one of the oldest known human diseases and a major cause of mortality around the world. The disease is initiated by the deposition of the bacteria, //Mycobacterium tuberculosis,// onto lung alveolar surfaces. It can also spread to other parts of the body such as the brain and spine in the central nervous system. Mycobacterium was first found in the soil in which it was transported to cattle as //Mycobacterium bovis.// Eventually, through the domestication of cattle, this bacteria was passed onto humans in the form of //Mycobacterium tuberculosis.// The most common symptoms include a bad cough, pain in the chest, and coughing up blood or sputum. In the present day, most cases are solved by antibiotics.
 * 1) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC164219/
 * 2) https://www.cdc.gov/features/tbsymptoms/index.html
 * 3) https://www.webmd.com/lung/understanding-tuberculosis-basics#1


 * Link to TDR Targets page (if present): **http://tdrtargets.org/targets/view?gene_id=5797
 * Links to Gene Database page: **
 * 1) []
 * 2) []
 * 3) []


 * <span style="font-family: Calibri,sans-serif;">Essentiality of protein: **<span style="font-family: Calibri,sans-serif;">essential; essentiality was unspecified
 * <span style="font-family: Calibri,sans-serif;">Is it a monomer or multimer as a biological unit? **<span style="font-family: Calibri,sans-serif;">dimer
 * <span style="font-family: Calibri,sans-serif;">Complex of proteins: **<span style="font-family: Calibri,sans-serif;">no
 * <span style="font-family: Calibri,sans-serif;">Druggable target: **<span style="font-family: Calibri,sans-serif;">0.5 from TDR targets
 * <span style="font-family: Calibri,sans-serif;">EC #: **<span style="font-family: Calibri,sans-serif;">3.6.3.8

[|http://www.brenda-enzymes.org/enzyme.php?ecno=3.6.3.8#REACTION]
 * <span style="font-family: Calibri,sans-serif;">Link to BRENDA EC # page: **

__<span style="font-family: Calibri,sans-serif;">SIGMA: __
 * <span style="font-family: Calibri,sans-serif;">Enzyme Assay: **<span style="font-family: Calibri,sans-serif;">S-Nitrosoglutathione
 * Cost: $177.00 for 25 mg/ $300.00 for 50 mg
 * Catalog #: N4148
 * 1) []
 * 2) []
 * 3) []

>
 * <span style="font-family: Calibri,sans-serif;">Structure (PDB): **<span style="font-family: Calibri,sans-serif;">3MD0
 * <span style="font-family: Calibri,sans-serif;">Current Inhibitors: **<span style="font-family: Calibri,sans-serif;"> [|http://www.brenda-enzymes.org/enzyme.php?ecno=3.6.3.8#INHIBITORS]
 * <span style="font-family: Calibri,sans-serif;">Expression Information (has it been expressed in bacterial cells): **<span style="font-family: Calibri,sans-serif;">Expressed in //Escherichia coli// BL21 (DE3) cells
 * 1) http://www.brenda-enzymes.org/enzyme.php?ecno=3.6.3.8#Cloned/COMMENTARY
 * <span style="font-family: Calibri,sans-serif;">Purification Method: **<span style="font-family: Calibri,sans-serif;">deoxycholate and salt fractionation
 * 1) []


 * <span style="font-family: Calibri,sans-serif;">Image of protein: **

<span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;">MKIPGVATVL GGVTNGVAQT VRAGARLPGS AAAAVQTLAS PVLELTGPVV <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;">QSVVQTTGRA IGVRGSHNES PDGMTPPVRW RSGRRVHFDL DPLLPFPRWH <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> EHAAMVEEPV RRIPGVAEAH VEGSLGRLVV ELEPDADSDI AVDEVRDVVS <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> AVAADIFLAG SVSSPNSAPF ADPGNPLAIL VPLTAAAMDL VAMGATVTGW <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> VARLPAAPQT TRALAALINH QPRMVSLMES RLGRVGTDIA LAATTAAANG <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> LTQSLGTPLL DLVQRSLQIS EAAAHRRVWR DREPALASPR RPQAPVVPII <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> SSAGAKSQEP RHSWAAAAAG EASHVVVGGS IDAAIDTAKG SRAGPVEQYV <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> NQAANGSLIA AASALVAGGG TEDAAGAILA GVPRAAHMGR QAFAAVLGRG <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> LANTGQLVLD PGALRRLDRV RVVVIDGAAL RGDNRAVLHA QGDEPGWDDD <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> RVYEVADALL HGEQAPEPDP DELPATGARL RWAPAQGPSA TPAQGLEHAD <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> LVVDGQCVGS VDVGWEVDPY AIPLLQTAHR TGARVVLRHV AGTEDLSASV <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> GSTHPPGTPL LKLVRELRAD RGPVLLITAV HRDFASTDTL AALAIADVGV <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> ALDDPRGATP WTADLITGTD LAAAVRILSA LPVARAASES AVHLAQGGTT <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> LAGLLLVTGE QDKTTNPASF RRWLNPVNAA AATALVSGMW SAAKVLRMPD <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> PTPQPLTAWH ALDPEIVYSR LAGGSRPLAV EPGIPAWRRI LDDLSYEPVM <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> APLRGPARTL AQLAVATRHE LADPLTPILA VGAAASAIVG SNIDALLVAG <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> VMTVNAITGG VQRLRAEAAA AELFAEQDQL VRRVVVPAVA TTRRRLEAAR <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> HATRTATVSA KSLRVGDVID LAAPEVVPAD ARLLVAEDLE VDESFLTGES <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> LPVDKQVDPV AVNDPDRASM LFEGSTIVAG HARAIVVATG VGTAAHRAIS <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> AVADVETAAG VQARLRELTS KVLPMTLAGG AAVTALALLR RASLRQAVAD <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> GVAIAVAAVP EGLPLVATLS QLAAAQRLTA RGALVRSPRT IEALGRVDTI <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> CFDKTGTLTE NRLRVVCALP SSTAAERDPL PQTTDAPSAE VLRAAARAST <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> QPHNGEGHAH ATDEAILAAA SALAGSLSSQ GDSEWVVLAE VPFESSRGYA <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> AAIGRVGTDG IPMLMLKGAP ETILPRCRLA DPGVDHEHAE SVVRHLAEQG <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;">LRVLAVAQRT WDNGTTHDDE TDADAVDAVA HDLELIGYVG LADTARSSSR <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> PLIEALLDAE RNVVLITGDH PITARAIARQ LGLPADARVV TGAELAVLDE <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> EAHAKLAADM QVFARVSPEQ KVQIVAALQR CGRVTAMVGD GANDAAAIRM <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> ADVGIGVSGR GSSAARGAAD IVLTDDDLGV LLDALVEGRS MWAGVRDAVT <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> ILVGGNVGEV LFTVIGTAFG AGRAPVGTRQ LLLVNLLTDM FPALAVAVTS <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> QFAEPDDAEY PTDDAAERAQ REHRRAVLIG PTPSLDAPLL RQIVNRGVVT <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;"> AAGATAAWAI GRWTPGTERR TATMGLTALV MTQLAQTLLT RRHSPLVIAT <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;">ALGSAGVLVG IIQTPVISHF SGVPRWDRSP GRASSAPRQE PPQSQRWHRS <span style="color: #222222; font-family: Courier,serif; font-size: 10.5pt;">GWQAQSVSCN LMNALTTRKT LTRVDRTYRR PR
 * <span style="font-family: Calibri,sans-serif;">Amino acid sequence: **


 * <span style="font-family: Calibri,sans-serif;">Length of protein in amino acids: **<span style="font-family: Calibri,sans-serif;">1632 aa
 * <span style="font-family: Calibri,sans-serif;">Molecular weight of protein: **<span style="font-family: Calibri,sans-serif;">169.61 kDa
 * <span style="font-family: Calibri,sans-serif;">Molar Extinction coefficient (280 nm wavelength): **<span style="font-family: Calibri,sans-serif;">128910 M-1cm-1


 * <span style="font-family: Calibri,sans-serif;">TMpred graph image: **

>NC_000962.3:125643-130541 Mycobacterium tuberculosis H37Rv CTACCGAGGGCGTCGATAGGTTCTATCCACCCGCGTCAGAGTCTTCCGCGTCGTCAGGGCGTTCATCAGG TTGCACGACACCGACTGTGCTTGCCAACCACTTCGGTGCCAGCGCTGAGACTGCGGTGGCTCCTGCCGTG GCGCTGAAGACGCCCGTCCAGGCGACCGGTCCCAGCGGGGTACACCCGAAAAGTGGCTGATAACCGGGGT TTGAATGATGCCGACCAACACCCCGGCGCTGCCCAGTGCGGTGGCAATGACGAGCGGACTGTGCCGGCGC GTCAGCAGTGTCTGCGCTAGCTGGGTCATCACGAGTGCAGTCAAACCCATTGTCGCCGTGCGTCGTTCGG TTCCGGGAGTCCAGCGCCCGATGGCCCAGGCTGCCGTTGCGCCGGCGGCGGTGACGACGCCGCGGTTAAC GATCTGACGCAGCAATGGCGCGTCCAGCGAGGGCGTAGGCCCGATCAGCACCGCACGTCGATGTTCGCGC TGCGCTCGCTCGGCCGCGTCATCGGTTGGGTATTCGGCGTCGTCGGGTTCGGCAAACTGCGAGGTGACGG CCACCGCAAGCGCGGGAAACATGTCGGTGAGCAGATTCACCAGCAGCAGTTGACGAGTCCCCACCGGCGC CCGCCCGGCCCCGAACGCCGTCCCGATGACGGTGAACAGAACTTCGCCCACATTGCCGCCGACCAGAATC GTCACCGCGTCACGAACACCGGCCCACATGCTGCGGCCCTCGACCAGCGCGTCGAGCAGCACGCCCAGGT CATCGTCGGTCAGCACGATATCGGCGGCCCCACGGGCGGCAGAGGAACCGCGCCCGCTCACTCCGATGCC CACGTCGGCCATCCGGATGGCCGCGGCGTCGTTGGCGCCGTCGCCGACCATCGCGGTCACTCGCCCGCAG CGCTGCAGCGCCGCCACAATCTGAACCTTTTGTTCCGGGCTGACCCGAGCAAAGACTTGCATGTCGGCGG CGAGTTTGGCATGCGCCTCCTCGTCCAGGACGGCAAGTTCGGCACCGGTCACGACTCGCGCGTCCGCCGG TAGTCCCAGCTGGCGGGCGATCGCCCGGGCGGTGATCGGATGGTCGCCGGTGATCAGCACCACGTTGCGC TCGGCGTCCAGCAAGGCTTCGATCAACGGACGCGAGGAAGACCGGGCCGTATCCGCCAATCCGACATAGC CGATCAGCTCGAGATCGTGCGCGACGGCGTCGACAGCGTCGGCGTCGGTCTCGTCATCATGGGTGGTCCC GTTGTCCCAGGTGCGCTGCGCGACTGCCAGAACACGCAGGCCCTGCTCGGCGAGGTGGCGTACCACGGAT TCGGCATGTTCGTGGTCGACGCCCGGGTCGGCGAGTCGGCAGCGCGGCAGGATCGTCTCCGGAGCGCCCT TGAGCATCAACATCGGTATCCCGTCGGTGCCCACTCTGCCGATCGCGGCGGCGTAGCCGCGACTGGACTC AAACGGTACTTCGGCCAGCACCACCCACTCCGAATCGCCTTGGCTACTAAGCGAACCGGCCAGCGCACTA GCCGCCGCGAGGATCGCCTCATCGGTGGCGTGCGCGTGCCCTTCCCCGTTATGGGGCTGCGTGGACGCGC GCGCGGCGGCCCGCAGCACCTCGGCGGAGGGCGCATCGGTGGTCTGCGGCAACGGATCCCGTTCGGCTGC GGTGCTGCTCGGTAGCGCGCATACCACCCGCAGGCGGTTCTCGGTGAGTGTGCCGGTCTTGTCGAAACAT ATGGTGTCGACACGGCCCAGCGCCTCGATGGTGCGAGGCGAGCGCACCAGCGCCCCACGTGCCGTCAGGC GCTGGGCGGCGGCAAGCTGGGAGAGGGTGGCCACCAACGGTAGACCCTCCGGGACCGCGGCCACCGCGAT GGCGACGCCGTCGGCCACCGCTTGCCGCAGCGACGCCCGGCGCAGCAACGCCAGAGCTGTCACCGCGGCG CCGCCGGCCAACGTCATGGGCAGCACTTTGCTGGTCAGCTCGCGCAGCCGGGCCTGGACTCCGGCCGCCG TTTCGACATCGGCGACCGCCGAGATCGCGCGATGTGCGGCGGTGCCGACTCCGGTGGCTACCACGATCGC GCGGGCGTGTCCGGCGACGATGGTGCTGCCCTCAAACAGCATGCTGGCCCGGTCGGGGTCGTTGACGGCG ACGGGGTCCACCTGCTTGTCCACCGGTAGCGACTCGCCGGTAAGAAAGGACTCGTCGACCTCGAGGTCTT CGGCCACCAGCAGGCGCGCATCCGCCGGGACCACCTCCGGCGCGGCCAGGTCGATGACATCGCCGACTCG CAGCGACTTCGCCGACACCGTGGCCGTCCGGGTGGCGTGCCGGGCCGCCTCCAGTCGACGTCGGGTAGTC GCTACCGCCGGCACCACCACCCGGCGCACCAGCTGGTCCTGCTCGGCGAATAGCTCGGCGGCCGCCGCCT CGGCTCGCAATCGTTGTACCCCACCGGTGATCGCGTTGACCGTCATCACGCCCGCTACCAGTAGCGCGTC GATATTGCTGCCGACAATCGCCGATGCTGCGGCGCCCACCGCCAGGATCGGAGTCAGCGGATCGGCCAGT TCATGGCGGGTGGCCACCGCCAGCTGCGCCAAGGTTCGTGCCGGGCCGCGCAGCGGCGCCATCACCGGTT CGTAGGACAGGTCGTCCAGAATGCGCCGCCAGGCCGGGATTCCGGGTTCGACGGCCAAGGGTCGGGAGCC GCCGGCTAGCCGCGAGTAGACGATCTCGGGGTCCAGCGCGTGCCAGGCGGTCAGCGGTTGCGGGGTGGGG TCGGGCATCCGCAGCACCTTGGCGGCCGACCACATTCCGGACACCAAAGCCGTTGCGGCAGCGGCATTGA CCGGATTGAGCCAGCGACGGAAGCTGGCTGGGTTGGTGGTTTTGTCCTGCTCACCGGTGACCAACAACAG CCCGGCCAAGGTGGTGCCACCTTGGGCGAGGTGTACCGCGGATTCACTGGCTGCCCGGGCCACCGGAAGC GCTGACAGGATCCGCACCGCCGCGGCCAGATCGGTGCCGGTGATTAGGTCGGCAGTCCATGGTGTTGCCC CGCGGGGATCGTCGAGAGCCACACCGACGTCAGCGATGGCCAACGCGGCCAACGTATCGGTGGATGCGAA GTCCCGGTGCACCGCGGTGATCAGCAATACCGGTCCGCGATCCGCGCGCAACTCACGCACCAACTTCAGC AACGGCGTGCCAGGCGGATGCGTCGAACCGACGCTGGCCGATAGATCTTCGGTGCCCGCGACATGGCGCA AAACCACCCGCGCTCCGGTTCGGTGCGCGGTCTGCAGCAGCGGGATTGCGTATGGGTCGACTTCCCACCC CACGTCGACGCTGCCCACGCATTGGCCATCGACCACCAGGTCGGCATGCTCGAGGCCCTGAGCCGGCGTT GCCGACGGCCCTTGAGCCGGAGCCCATCTCAAGCGGGCACCGGTAGCCGGCAATTCATCGGGGTCGGGTT CGGGTGCCTGCTCGCCATGGAGCAAGGCGTCGGCGACCTCGTAGACGCGGTCGTCGTCCCAGCCGGGTTC GTCTCCCTGTGCATGCAATACGGCGCGGTTGTCACCGCGCAGCGCTGCGCCGTCGATAACGACCACCCGC ACCCGATCCAGGCGGCGCAACGCGCCGGGGTCGAGGACTAGTTGCCCGGTGTTGGCGAGCCCGCGACCCA GCACCGCCGCGAACGCCTGTCGGCCCATGTGCGCGGCACGTGGTACTCCGGCCAGGATCGCGCCGGCCGC GTCCTCGGTACCACCGCCGGCCACCAGTGCACTGGCCGCGGCGATCAACGAACCGTTTGCGGCCTGGTTG ACGTATTGCTCCACCGGCCCGGCCCTTGACCCTTTGGCGGTGTCGATCGCGGCGTCGATGGATCCGCCGA CCACGACGTGCGAAGCCTCGCCTGCCGCCGCAGCCGCCCAACTGTGTCGCGGCTCCTGCGATTTGGCCCC GGCCGACGAGATGATGGGCACCACCGGAGCCTGCGGCCGTCTGGGGGAGGCCAGCGCGGGTTCGCGGTCA CGCCATACGCGACGGTGCGCCGCCGCTTCGGAGATTTGCAGGCTGCGTTGCACCAGATCGAGCAGCGGTG TGCCCAGGGACTGGGTTAGCCCGTTGGCCGCCGCCGTCGTAGCGGCGAGCGCAATATCGGTGCCCACTCG ACCCAACCGCGACTCCATAAGCGACACCATTCGCGGTTGATGGTTTATCAGAGCGGCCAGGGCTCTGGTG GTTTGCGGTGCGGCGGGCAGTCGGGCGACCCAGCCGGTGACCGTGGCACCCATCGCTACCAGATCCATTG CCGCAGCGGTCAACGGCACCAGGATCGCCAAGGGGTTACCTGGGTCGGCGAATGGTGCCGAGTTCGGCGA CGACACCGACCCAGCCAGGAATATGTCGGCAGCCACCGCGGAAACCACATCACGTACCTCGTCCACGGCG ATGTCGCTATCCGCATCAGGTTCAAGTTCGACCACCAGCCGACCCAATGAGCCCTCAACGTGGGCCTCGG CCACGCCCGGGATCCTGCGGACTGGCTCCTCCACCATGGCGGCATGCTCGTGCCAGCGAGGGAATGGCAG TAGCGGATCCAGGTCGAAATGCACGCGCCGTCCGCTGCGCCAACGCACCGGCGGTGTCATTCCGTCAGGC GATTCGTTGTGGGAACCGCGTACCCCGATTGCGCGACCAGTCGTTTGCACCACCGACTGCACCACCGGGC CGGTCAGCTCCAGCACCGGGCTGGCCAGCGTCTGCACCGCCGCGGCCGCACTCCCCGGCAAGCGGGCGCC GGCTCGCACTGTCTGTGCTACTCCGTTGGTCACACCACCGAGGACAGTGGCCACACCCGGGATCTTCAC
 * <span style="font-family: Calibri,sans-serif;">CDS Gene Sequence: **


 * <span style="font-family: Calibri,sans-serif;">GC% Content for gene: **<span style="font-family: Calibri,sans-serif;">58%
 * <span style="font-family: Calibri,sans-serif;">CDS Gene Sequence (codon optimized): **<span style="font-family: Calibri,sans-serif;">N/A
 * <span style="font-family: Calibri,sans-serif;">GC% Content for gene (codon optimized): **<span style="font-family: Calibri,sans-serif;">N/A
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **N/A
 * Primer design results for 'tail' primers (this is just 2 sequences): ** N/A