Target-MPTPB(Mycobacterium+Tuberculosis)+H37Rv

Organism: MycoBacterium Tuberculosis H37Rv

Gene of Interest: MptpB Rv0153c

Background/Disease:

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NCBI Gene # or RefSeq#: NP_214667.1

EC# of Enzyme: 3.1.3.48

CAS Registry Number: 208861-82-9

CDS Gene Sequence:

ATGGCGGTTCGTGAACTGCCGGGTGCGTGGAACTTCCGCGACGTGGCTGACACCGCGACC GCGCTGCGTCCGGGTCGTCTGTTCCGTAGCTCTGAACTGTCTCGTCTCGATGATGCCGGT CGTGCCACCCTGCGCCGTCTGGGTATCACCGACGTTGCCGATCTCCGCTCTTCCCGTGAA GTTGCTCGTCGTGGTCCAGGTCGTGTTCCAGATGGTATCGACGTTCACCTGCTGCCGTTT CCTGATCTCGCGGACGATGACGCGGATGACTCTGCGCCTCACGAAACCGCATTCAAACGT CTGCTGACCAACGACGGCTCTAATGGCGAATCTGGTGAATCTTCTCAGTCTATCAATGAT GCAGCCACTCGTTACATGACCGACGAATATCGTCAATTCCCGACCCGTAATGGTGCGCAG CGTGCCCTGCATCGTGTTGTAACCCTCCTGGCCGCTGGCCGTCCGGTTCTGACCCACTGC TTTGCGGGTAAAGACCGTACCGGCTTCGTTGTTGCACTGGTTCTGGAAGCGGTTGGTCTG GATCGTGACGTAATTGTGGCGGACTACCTCCGTTCTAACGACAGCGTTCCGCAACTGCGT GCGCGTATCTCTGAAATGATCCAGCAGCGTTTCGACACCGAACTGGCACCGGAGGTTGTT ACCTTCACCAAAGCGCGTCTGTCTGACGGCGTTCTCGGTGTTCGTGCGGAATACCTGGCA GCGGCTCGTCAAACCATCGACGAAACTTACGGTTCTCTGGGTGGTTACCTGCGTGATGCG GGCATCTCTCAGGCGACCGTTAACCGTATGCGTGGTGTTCTCCTGGGTTAA

Amino Acid Sequence (with stop codon, X, added at the end):

MptpB Rv0153c [] >M. tuberculosis H37Rv | ptbB MAVRELPGAWNFRDVADTATALRPGRLFRSSELSRLDDAGRATLRRLGITDVADLRSSREVARRGPGRVPDGIDVHLLPF PDLADDDADDSAPHETAFKRLLTNDGSNGESGESSQSINDAATRYMTDEYRQFPTRNGAQRALHRVVTLLAAGRPVLTHC FAGKDRTGFVVALVLEAVGLDRDVIVADYLRSNDSVPQLRARISEMIQQRFDTELAPEVVTFTKARLSDGVLGVRAEYLA AARQTIDETYGSLGGYLRDAGISQATVNRMRGVLLGX

Length of protein in Amino Acids: 276

Molecular Weight of recombinant protein with MHHHHHHSSGVDLGTENLYFQSM N-terminal sequence: 32.71 kDa Primer design results for pNIC-Bsa4 cloning:

Forward Primer:

TACTTCCAATCCATGGCGGTTCGTGAACTGCC

Reverse Primer:

TATCCACCTTTACTG TTAACCCAGGAGAACACCAC