Brian+C.

Good results page Brian. - Dr. B 071713
 * Week 7 - 7/15/2013**
 * __//HAPPY BIRTHDAY TO ME!//__**

DNA Sequencing Results for last week's FabV plasmid are in:

Using PLic Forward:

NNNNNNNNGTNNACTTTANNNGAGANATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGGGTACCGAG AACCTGTACTTCCAATCCATGATTATCAAGCCGCGTGTACGTGGTTTTATCTGTGTTACTGCGCATCCAACGGGTTGCGA GGCTAACGTGAAAAAACAGATCGACTACGTTACCACCGAAGGTCCGATTGCGAACGGTCCGAAACGTGTTCTGGTTATTG GTGCGTCTACCGGTTACGGTCTGGCAGCCCGTATCACGGCGGCTTTTGGTTGCGGCGCAGATACCCTGGGCGTTTTCTTC GAACGTCCTGGCGAAGAAGGCAAACCGGGTACGTCTGGTTGGTACAATTCTGCGGCGTTCCACAAATTCGCTGCGCAAAA AGGTCTGTACGCGAAATCTATCAACGGTGATGCGTTCTCTGACGAAATTAAGCAGCTCACCATTGACGCGATCAAACAGG ACCTCGGTCAGGTTGACCAAGTTATTTACTCTCTCGCGTCTCCACGTCGTACCCACCCGAAGACCGGTGAAGTTTTCAAC TCTGCGCTGAAACCTATTGGTAATGCTGTAAATCTGCGTGGCCTGGACACCGACAAAGAAGTTATCAAAGAATCTGTCCT GCAGCCGGCGACCCAGTCTGAAATCGACTCTACCGTTGCGGTCATGGGCGGTGAGGATTGGCAAATGTGGATCGATGCCC TCCTGGACGCGGGTGTTCTCGCGGAAGGTGCCCAAACCACCGCGTTCACCTACCTGGGTGAGAAAATCACGCACGATATC TATTGGAACGGTTCTATCGGTGCGGCGAAAAAAGACCTGGACCAGAAAGTTCTGGCGATCCGTGAATCTCTGGCGGCTCA CGGTGGCGGCGATGCTCGTGTTTCTGTTCTGAAAGCGGTTGTTACCCAGGCGTCTTCTGCGATCCCTATGATGCCGCTCT ACCTGTCTCTGCTNNTCAAANNTTNTGAAAANNNNAANNNACNCATGANNNTGCATCGANNNGTCTACNCCCNNNNCNAA NNATTNNNNNNNNNNCNGNNNATNNNNCCNNNNNATGGGANNNNAANNCGTNNNCNNNNNGNNTANNNNNCTNNNNCNNN NNTCNNANCNGNNCANNANCTNNNNGNNNNNNANNNNANANNNTNNNNNNCNNANNNNTNNNNGNNNNNNANNNNNANNN NNCNNTNNNNNNNNNNNNNNNGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN

Using PLic Reverse:

NNNNNNNNNNNNGGNNCTCNGTGGTGGTGGTGGTGGTGCTCGAGTGCGGCCGCAAGCTTGTCGACGGAGCTCGAATTCGG ATCCGTATCCACCTTTACTGTTAACCCTGGATCAGATTCGGGATTTTGACGTCCGGGTTAACATCCGCGTCGTAGTCAAC ACCGTCGATACCGAAACCGAAGAGGTTGAGGAATTCAGATTTGTAACCCACGAAATCCGTCAGTTGGTAGATGTTGTCGT TGGTCACCTGGTCCCACAGCTGCTGAACCTGATTCTGGACTTCAGGGTCGAGCTCCTTGTAGTCCGCACGGAGACGACCT TCTTGGTCCATGTGCGGAGAATCACCGCACAGAGAATCTTTGTAGAGGGAGTAGACCTGCTCGATGCAACCTTCATGCGT ACCTTTCTCTTTCATAACTTTGAACAGCAGAGACAGGTAGAGCGGCATCATAGGGATCGCAGAAGACGCCTGGGTAACAA CCGCTTTCAGAACAGAAACACGAGCATCGCCGCCACCGTGAGCCGCCAGAGATTCACGGATCGCCAGAACTTTCTGGTCC AGGTCTTTTTTCGCCGCACCGATAGAACCGTTCCAATAGATATCGTGCGTGATTTTCTCACCCAGGTAGGTGAACGCGGT GGTTTGGGCACCTTCCGCGAGAACACCCGCGTCCAGGAGGGCATCGATCCACATTTGCCAATCCTCACCGCCCATGACCG CAACGGTAGAGTCGATTTCAGACTGGGTCGCCGGCTGCAGGACAGATTCTTTGATAACTTCTTTGTCGGTGTCCAGGCCA CGCAGATTTACAGCATTACCAATAGGTTTCAGCGCAGAGTTGAAAACTTCACCGGTCTTCGGGTGGGTACGACGTGGAGA CGCGAGAGAGTAAATAACTTGGTCAACCTGACCGAGGTCCTGTTTGATCGCGTCAATGGTGAGCTGCTTAATTTCGTCAG ANAACGCATCANCNNNGATAGATTTCGCGTACAGACCNTTTTTGCGCANCGAATTNNNGGNNNNCGCANANNGTACCNAC CAGANGTTNCCCNGNTTGNNTNNNTCNCCNGGNCNTTCNANAAAACGCCCAGGGNNNNNGCNNCNCANNAANCNNNNGAT NNGGCNNCNNACGNNNNNNNNANNNNNCNNNNNNAANNNNNTTCGNNCNNNNNNANNNNANNNNNNGGNANNNNNNNANN NNNTTTNNNNNNTNNCNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNTAANNNNNNN

(Preexisting) Codon Optimized Sequence: __ATGATTATCAAGCCGCGTGT__ACGTGGTTTTATCTGTGTTACTGCGCATCCAACGGGTTGCGAGGCTAACGTGAAAAAACAGATCGACTACGTTACCACCGAAGGTCCGATTGCGAACGGTCCGAAACGTGTTCTGGTTATTGGTGCG

BLAST Alignment Results:


 * Week 6 - 7/8/2013**

Transformation and Midi Prep results for FabV. Used 1ml of buffer TE instead of 0.5ml.





Average concentration: 21.5 ng/uL

Submitted to sequencing 7/11/2013 with order number 93094


 * Week 5 - 7/1/2013**



Lane 1: 100bp DNA Ladder Lane 2: Sample A Lane 2: Sample B Lane 3: Sample C Lane 4: Sample D (Control, contamination check)


 * Week 4 - 6/24/2013**


 * 1) Skipped
 * 2) 1kb Ladder
 * 3) Uncut Plasmid (Ashlee's)
 * 4) EcoRI (Ashlee's)
 * 5) EcoRI + PvuII (Ashlee's)
 * 6) PVUII (Ashlee's)
 * 7) Uncut Plasmid
 * 8) EcoRI
 * 9) PvuII
 * 10) EcoRI + PvuII

Virtual Gels: