XENIA+G.

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__ Materials & Methods: __ __ Results: __
 * Figure 1(a&b):** Front and back view of plate with bacteria only (VDS JWS XBG 2/27/14 NONE BL21(DE3)) the evening of Day 2. No bacterial growth evident.


 * Figure 2(a&b):** Front and back view of plate with bacteria and ampicillin-resistant, purple encoding protein plasmid (VDS JWS XBG 2/27/14 AMP BL21(DE3) pGEM-gbr22) the evening of day 2. No bacterial growth evident.


 * Figure 3:** VDS JWS XBG 2/27/14 FUN plate with bacteria from coughing. Photo was taken evening of day 2. No bacterial growth was evident.


 *  Figure 4: ** "VDS ARW AL 2/27/14 BL21(DE3) SOC media NO DNA" plate with bacteria only. Photo taken evening of day 2. No bacterial growth evident.


 * Figure 5: **"VDS ARW AL 2/27/14 BL21(DE3) pGEM-gbr22 soc media" plate with bacteria and ampicillin-resistant, purple encoding protein plasmid inserted in bacteria. Photo taken evening of day 2. Bacterial growth was evident with many single colonies present.


 * Figure 6 (a&b):** Flasks containing LB, ampicillin, BL21(DE3)+pGEM-gbr22 (VDS xbg 2/28/14), covered with foil after spending 24hrs in shaking incubator (37 degrees Celsius & 200-350 rpm). Photo taken 6:10pm on day 3. 5a is one flask with yellow media, indicating no bacterial growth. 5b is the other flask with purple media, indicating bacterial growth.


 * Figure 7:** Wet pellet of bacteria (BL21(DE3)) with purple protein (encoded by plasmid pGEM-gbr22). Photo taken day 3 after centrifuge and disposing of extra liquid media.


 * Figure 8:** "Sample 5" indicates the Elution 1 tube, containing the majority of the purified gbr22 protein (evident by purplish color). "Sample 6" indicates the Elution 2 tube, containing some purified gbr22 protein (evident by more clear color).


 * Figure 9.** Trial 1 of absorbance reading of Elution 1 solution by nanodrop spectrophotometer at 280nm, using Protein - A280 mode. The absorbance shows to be 0.237 A280.


 * Figure 10.** Trial 2 of absorbance reading of Elution 1 solution by nanodrop spectrophotometer at 280nm, using Protein - A280 mode. The absorbance shows to be 0.237 A280.


 * Figure 11.** Trial 1 of absorbance reading of Elution 1 solution by nanodrop spectrophotometer at 574nm as well as at 280nm, using UV/VIS mode. Because this mode uses a 1mm path length instead of 1cm, the absorbance reading at 574 nm, multiplied by 10, is 0.18 A574 (while for 280nm, it is 0.33 A574).


 * Figure 12.** Trial 2 of absorbance reading of Elution 1 solution by nanodrop spectrophotometer at 574nm as well as at 280nm, using UV/VIS mode. Because this mode uses a 1mm path length instead of 1cm, the absorbance reading at 574 nm, multiplied by 10, is 0.14 A574 (while for 280nm, it is 0.31 A574).


 * Figure 13.** Gel of molecular weight standards (1st column; ColorPlus Prestained Lot#0131303, New England Biolabs), another student's samples 1-6 (columns 2-7), and my samples 4-6 (columns 8-10), before drying.




 * Figure 14.** Gel of molecular weight standards (1st column; ColorPlus Prestained Lot#0131303, New England Biolabs), another student's samples 1-6 (columns 2-7), and my samples 4-6 (columns 8-10), after drying. Both sample sets were labeled based on what was contained: cell lysate in column 2, soluble fraction in column 3, flow through in column 4, wash in column 5, elution 1 in column 6, elution 2 in column 7, wash in column 8, elution 1 in column 9, and elution 2 in column 10. The molecular weight standards measurements/indicators (ColorPlus Prestained Log #0131303, New England Biolabs) are shown to the left of the gel.

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