Target+-+Secreted+Phosphatase+(Francisella+tularensis)


 * Target (protein/gene name):** Secreted Phosphatase


 * NCBI Gene # or RefSeq#:** FTN_0090


 * *Protein ID (NP or XP #) or Wolbachia#: **YP_897755.1


 * Organism (including strain):** Francisella tularensis

Persons living or visiting in areas with high concentrations of ticks Persons who have hobbies or work hunting, trapping, gardening, or landscaping Persons who work in wildlife management or veterinary medicine
 * Etiologic Risk Group:**

Francisella tularensis causes the disease Tularemia which is a zoonotic disease commonly contracted by insect bites and contact with infected animals. This disease can attack various organ systems including the skin, eyes, lymph nodes, and lungs. The symptoms of tularemia vary depending on where the bacteria enters the body. Ulceroglandular tularemia, the most common form of the disease, causes symptoms such as: an ulcer at the site of infection, swollen or painful lymph glands, fever, chills, headache, and exhaustion. Any symptoms will usually begin 2-10 days after infection. If left untreated, Tularemia can lead to pneumonia, pericarditis, meningitis, and osteomyelitis. This disease is highly contagious, can be fatal, and can be potentially used for bio-terrorism. These factors create a need for more efficient drugs. Currently, tularemia is treated with antibiotics such as streptomycin and gentamicin. Since antibiotics can lead to various side effects and possible drug resistance, more efficient drugs are necessary.
 * */Disease Information (sort of like the Intro to your Mini Research Write up): **


 * Link to TDR Targets page (if present):** n/a

[]
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.): **


 * Essentiality of this protein:** Known to be essential


 * Is it a monomer or multimer as biological unit? Most Probable Assembly:** 2-mer


 * Complex of proteins?:** Complexed with Othrovanadate (2D1G)


 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):** n/a (Could not be found)

** *EC#: ** 3.1.3.2

 * Link to BRENDA EC# page:** @http://www.brenda-enzymes.org/php/result_flat.php4?ecno=3.1.3.2

** Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**
Phosphatase assay was performed in 20 mM sodium acetate buffer, 1 mM MgCl2 pH 6 (assay buffer), at 37 °C, using as substrate 1 mM pNPP, in a volume of 250 μl. @http://www.sciencedirect.com/science/article/pii/S1570963905003699

[] + Zn2+, vanadate, tungstate, Tartrate, orthovanadate, molybdate, Hg2+, FeSO4, Cu2+, arsenate, Ag+, (AgNO3)
 * Structure (PDB or Homology model):** 2DIG
 * Current Inhibitors:**

[]
 * Expression Information (has it been expressed in bacterial cells):** Has been expressed in //E. coli// Dh5a cells.


 * Purification Method :** Chromatography using a DEAE-Sepharose FF column

MKLNKITLGILSLSIATTTFATDVNNSKPNDYGTLVKIEQKLFNNANTLKTTTPIKHVVIIFQENNSFDRYFGMYPNAKNPEGEPKFVAKENTPNVNGLTKQLLENNPNTKNPYRLDRNFQPCSQNHEYHQEISSFNGGLMNKFVEHGGHDNDTYKQNCDGQVMGYYDGNTVTALWNYAQNFALNDNTFGTTFGPSTPGALNLVAGANGPAMSPSGNLENIENNYIIDDPNPYYDDCSYGTSKSGDTNTAVAKITDGYNIGHYLTQKGITWGWFQGGFKPTSYSGKTAICDAMSTNKFGVKSRDYIPHHEPFNYWKETSNPHHLAPSDDKYIGSNDQANHQYDISEFWKALDQNNMPAVSYLKAPGYQDGHGGYSNPLDEQEWLVNTINRIQQSKDWDSTAIIIIYDDSDGDYDHVYSPKSQFSDIKGRQGYGPRLPMLVISPYAKANYVDHSLLNQASVLKFIEYNWGIGSVSKYSNDKYSNNILNMFDFNKEQKTLKLILDPKTGLVMDKLN
 * Image of protein (PyMol with features delineated and shown separately):**
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):**


 * length of your protein in Amino Acids: 514**


 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website: 57780.9 kDa**

code Ext. coefficient   91930 Abs 0.1% (=1 g/l)  1.591, assuming all pairs of Cys residues form cystines
 * Molar Extinction coefficient of your protein at 280 nm wavelength:**

Ext. coefficient   91680 Abs 0.1% (=1 g/l)  1.587, assuming all Cys residues are reduced code 2 Possible Transmembrane Regions
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html).

ATGGACGTGAATAATTCTAAGCCGAACGATTACGGTACCCTGGTTAAAATCGAACAGAAACTGTTTAACAACGCGAATACGCTCAAAACGACCACCCCGATTAAACACGTAGTCATTATCTTCCAGGAGAATAACTCTTTTGATCGTTACTTCGGTATGTACCCGAATGCGAAGAATCCGGAAGGTGAACCAAAGTTTGTTGCGAAAGAAAACACCCCAAATGTTAACGGCCTCACCAAACAGCTGCTCGAAAATAACCCGAACACCAAAAATCCGTACCGCCTGGATCGCAACTTCCAGCCGTGCTCTCAGAACCACGAATACCACCAGGAAATCTCTTCTTTCAATGGTGGCCTGATGAATAAGTTCGTCGAACATGGTGGTCACGACAATGACACCTACAAACAGAATTGCGATGGTCAGGTGATGGGCTACTATGATGGCAATACTGTTACCGCGCTGTGGAATTACGCGCAGAACTTCGCGCTGAATGACAACACCTTTGGTACTACCTTCGGTCCGTCTACCCCGGGTGCCCTGAACCTGGTTGCGGGTGCAAACGGTCCGGCGATGTCTCCGTCTGGCAACCTCGAGAACATCGAAAACAACTACATCATTGACGACCCTAACCCGTATTATGACGACTGCTCTTACGGCACCTCTAAATCTGGCGACACCAACACTGCGGTTGCTAAGATCACGGATGGTTACAACATCGGTCACTACCTCACCCAAAAAGGTATCACTTGGGGTTGGTTTCAAGGCGGCTTCAAACCGACCTCTTACTCTGGTAAGACGGCGATTTGTGACGCCATGTCTACCAACAAGTTCGGTGTTAAATCTCGTGACTATATCCCGCACCACGAGCCTTTCAACTATTGGAAGGAGACCTCCAATCCACACCACCTCGCGCCGAGCGACGACAAATACATTGGTTCTAATGATCAAGCGAATCACCAATACGACATCTCTGAATTCTGGAAGGCGCTCGACCAGAACAACATGCCTGCCGTTTCTTACCTGAAAGCTCCGGGTTACCAGGATGGCCACGGTGGCTACTCCAACCCACTGGACGAACAGGAATGGCTCGTTAATACCATCAACCGTATCCAGCAGTCTAAAGACTGGGACTCTACCGCTATTATCATCATTTACGATGACAGCGACGGTGATTACGATCACGTTTACTCTCCAAAGTCTCAATTCTCTGACATCAAGGGCCGTCAAGGTTATGGTCCGCGTCTGCCGATGCTGGTTATCAGCCCGTATGCAAAAGCTAACTACGTTGACCACTCTCTGCTGAATCAGGCGTCTGTCCTGAAATTCATCGAATACAACTGGGGTATCGGTTCCGTATCTAAATACTCTAACGACAAGTATTCTAACAATATCCTGAATATGTTTGACTTCAACAAAGAGCAAAAAACCCTGAAACTGATCCTGGACCCGAAAACTGGTCTCGTTATGGATAAACTGAACTAA
 * CDS Gene Sequence (paste as text only):**
 * GC% Content for gene:** 47.77

FILTERED DNA SEQUENCE TACTTCCAATCCATG __ GACGTGAATAATTCTAA __ GCCGAACGATTACGGTACCCTGGTTAAAATCGAACAGAAA CTGTTTAACAACGCGAATACGCTCAAAACGACCACCCCGATTAAACACGTAGTCATTATC TTCCAGGAGAATAACTCTTTTGATCGTTACTTCGGTATGTACCCGAATGCGAAGAATCCG GAAGGTGAACCAAAGTTTGTTGCGAAAGAAAACACCCCAAATGTTAACGGCCTCACCAAA CAGCTGCTCGAAAATAACCCGAACACCAAAAATCCGTACCGCCTGGATCGCAACTTCCAG CCGTGCTCTCAGAACCACGAATACCACCAGGAAATCTCTTCTTTCAATGGTGGCCTGATG AATAAGTTCGTCGAACATGGTGGTCACGACAATGACACCTACAAACAGAATTGCGATGGT CAGGTGATGGGCTACTATGATGGCAATACTGTTACCGCGCTGTGGAATTACGCGCAGAAC TTCGCGCTGAATGACAACACCTTTGGTACTACCTTCGGTCCGTCTACCCCGGGTGCCCTG AACCTGGTTGCGGGTGCAAACGGTCCGGCGATGTCTCCGTCTGGCAACCTCGAGAACATC GAAAACAACTACATCATTGACGACCCTAACCCGTATTATGACGACTGCTCTTACGGCACC TCTAAATCTGGCGACACCAACACTGCGGTTGCTAAGATCACGGATGGTTACAACATCGGT CACTACCTCACCCAAAAAGGTATCACTTGGGGTTGGTTTCAAGGCGGCTTCAAACCGACC TCTTACTCTGGTAAGACGGCGATTTGTGACGCCATGTCTACCAACAAGTTCGGTGTTAAA TCTCGTGACTATATCCCGCACCACGAGCCTTTCAACTATTGGAAGGAGACCTCCAATCCA CACCACCTCGCGCCGAGCGACGACAAATACATTGGTTCTAATGATCAAGCGAATCACCAA TACGACATCTCTGAATTCTGGAAGGCGCTCGACCAGAACAACATGCCTGCCGTTTCTTAC CTGAAAGCTCCGGGTTACCAGGATGGCCACGGTGGCTACTCCAACCCACTGGACGAACAG GAATGGCTCGTTAATACCATCAACCGTATCCAGCAGTCTAAAGACTGGGACTCTACCGCT ATTATCATCATTTACGATGACAGCGACGGTGATTACGATCACGTTTACTCTCCAAAGTCT CAATTCTCTGACATCAAGGGCCGTCAAGGTTATGGTCCGCGTCTGCCGATGCTGGTTATC AGCCCGTATGCAAAAGCTAACTACGTTGACCACTCTCTGCTGAATCAGGCGTCTGTCCTG AAATTCATCGAATACAACTGGGGTATCGGTTCCGTATCTAAATACTCTAACGACAAGTAT TCTAACAATATCCTGAATATGTTTGACTTCAACAAAGAGCAAAAAACCCTGAAACTGATC CTGGACCCGAAAACTGGTCTCGTTATGGATAAACTGAACTAACAGTAAAGGTGGATA
 * CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):**


 * GC% Content for gene (codon optimized):** 47.65


 * Primer design results for pNIC-Bsa4 cloning (list sequences of all of your ~40 nt long primers):**




 * [|DNA Works Output] **


 * Primer design results for 'tail' primers (this is just 2 sequences):**
 * Forward Primer:** TACTTCCAATCCATGGACGTGAATAATTCTAA
 * Reverse Primer:** TATCCACCTTTACTGTTAGTTCAGTTTATCCATAA