Dihydropteroate+synthase Mycobacterium+Leprae

Group 3 http://www.bacterio.net/-hazard.html#group3
 * Disease:** Leprosy (Hanse's disease)
 * Enzyme class**: 2; Transferases
 * Target (protein/gene name):** //Dihydroteroate synthase//
 * NCBI Gene # or RefSeq#: --**
 * Protein ID (NP or XP #) or Wolbachia#: --**
 * Organism (including strain):** //Mycobacterium Leprae//
 * Etiologic Risk Group:**

Leprosy, which is also known as Hansen's disease is an infectious disease by //Mycobacterium leprae.// They can affect human in various ways by making human lose the ability to sense touch or pain. When injuries happen, people will not know if they are in danger because their first defense line is gone. They affect nerves, skin, eyes, and nasal mucosa. The affected area becomes either light or dark and become dry. They can also be inflamed. Without cure, they can lead to a paralysis of limps. They are contagious, but there is an easy cure to this disease now. However, in third world countries, leprosy is still around affecting many people. @https://www.cdc.gov/leprosy/
 * Disease Information :**


 * Link to TDR Targets page:** n/a
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.)**
 * Essentiality of this protein: --**

@https://www.ncbi.nlm.nih.gov/pubmed/10329458
 * Complex of proteins?: --**
 * Druggable Target:**

@http://www.brenda-enzymes.org/enzyme.php?ecno=2.5.1.15&UniProtAcc=P0C0X1&OrganismID=3504
 * EC#:** 2.5.1.15
 * Link to BRENDA EC# page:**

@https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/dihydropteroate-synthase
 * Enzyme Assay information:**


 * List cost and quantity of substrate reagents, supplier, and catalog #**
 * 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate

-- PDB #l: Q9R2U9
 * Structure**

@http://www.brenda-enzymes.org/enzyme.php?ecno=2.5.1.15&UniProtAcc=P0C0X1&OrganismID=3504
 * Current Inhibitors:**


 * Expression Information (has it been expressed in bacterial cells): --**
 * Purification Method : --**
 * Image of protein (PyMol with features delineated and shown separately):**

code format="sequence"        10         20         30         40         50 MSLAPVQVIG VLNVTDNSFS DGGRYLDPDD AVQHGLAMVA EGAAIVDVGG 60        70         80         90        100 ESTRPGAIRT DPRVELSRIV PVVKELAAQG ITVSIDTTRA DVARAALQSG 110       120        130        140        150 ARIVNDVSGG RADPAMAPLV AEAGVAWVLM HWRLMSAERP YEAPNYRDVV 160       170        180        190        200 AEVRADLLAG VDQAVAAGVD PGSLVIDPGL GFAKTGQHNW ALLNALPELV 210       220        230        240        250 ATGVPILLGA SRKRFLGRLL AGADGAVRPP DGRETATAVI SALAALHGAW 260       270        280 GVRVHDVRAS VDALKVVGAW LHAGPQIEKV RCDG code 284 29.448 kDa code Ext. coefficient   31970 Abs 0.1% (=1 g/l)  1.086, assuming all pairs of Cys residues form cystines
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):**
 * length of your protein in Amino Acids**
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website**
 * Molar Extinction coefficient of your protein at 280 nm wavelength:**

Ext. coefficient   31970 Abs 0.1% (=1 g/l)  1.086, assuming all Cys residues are reduced code atgagcctggcgccggtgcaggtgattggcgtgctgaacgtgaccgataacagctttagc gatggcggccgctatctggatccggatgatgcggtgcagcatggcctggcgatggtggcg gaaggcgcggcgattgtggatgtgggcggcgaaagcacccgcccgggcgcgattcgcacc gatccgcgcgtggaactgagccgcattgtgccggtggtgaaagaactggcggcgcagggc attaccgtgagcattgataccacccgcgcggatgtggcgcgcgcggcgctgcagagcggc gcgcgcattgtgaacgatgtgagcggcggccgcgcggatccggcgatggcgccgctggtg gcggaagcgggcgtggcgtgggtgctgatgcattggcgcctgatgagcgcggaacgcccg tatgaagcgccgaactatcgcgatgtggtggcggaagtgcgcgcggatctgctggcgggc gtggatcaggcggtggcggcgggcgtggatccgggcagcctggtgattgatccgggcctg ggctttgcgaaaaccggccagcataactgggcgctgctgaacgcgctgccggaactggtg gcgaccggcgtgccgattctgctgggcgcgagccgcaaacgctttctgggccgcctgctg gcgggcgcggatggcgcggtgcgcccgccggatggccgcgaaaccgcgaccgcggtgatt agcgcgctggcggcgctgcatggcgcgtggggcgtgcgcgtgcatgatgtgcgcgcgagc gtggatgcgctgaaagtggtgggcgcgtggctgcatgcgggcccgcagattgaaaaagtg cgctgcgatggc
 * TMpred graph Image** ( @http://www.ch.embnet.org/software/TMPRED_form.html ). Input your amino acid sequence to it.
 * CDS Gene Sequence (paste as text only):**


 * GC% Content for gene:** 69.014085
 * CDS Gene Sequence (codon optimized): n/a**
 * GC% Content for gene (codon optimized): n/a**

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)

**
 * Primer design results for 'tail' primers (this is just 2 sequences):**