Target+-+Fab+V+(Yersinia+Pestis)

FabV (Enoyl-ACP Reductase)
 * Target (protein/gene name):**

NZ_ACNR01000001.1
 * NCBI Gene # or RefSeq#:**

ZP_04462052.1
 * Protein ID (NP or XP #) or Wolbachia#:**

Yersinia Pestis biovar Orientalis str. India 195
 * Organism:**

India/Orientals
 * Etiologic Risk Group (see link below):**

http://gsc.jcvi.org/projects/msc/yersinia/yersinia_pestis_biovar_orientalis_str_f1991016/index.shtml http://gsc.jcvi.org/projects/msc/yersinia/yersinia_pestis_biovar_orientalis_str_ip275/index.shtml http://gsc.jcvi.org/projects/msc/yersinia/yersinia_pestis_biovar_orientalis_str_mg05-1020/index.shtml
 * Background/Disease Information:**

Cool link - Dr. B //Yersinia pestis// study - old plague not different from new plague.. @http://www.ncbi.nlm.nih.gov/pubmed/21993626

http://www.ncbi.nlm.nih.gov/pubmed?term=fabv%20yersinia%20pestis http://www.sciencedirect.com/science/article/pii/S0969212611004114 http://www.cell.com/structure/abstract/S0969-2126%2811%2900411-4
 * Essentiality of this protein:**

EC#: 1.3.1.-
 * Complex of enzymes:**

http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.3.1.-
 * Link to BRENDA EC# page:**

Crotonoyl coenzyme A trilithium salt (for fabg) 28007-5MG -246.00 http://www.sigmaaldrich.com/catalog/search?interface=All&term=crotonoyl-CoA〈=en®ion=US&focus=product&N=0+220003048+219853269+219853286&mode=mode%20matchpartialmax
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**
 * -- link to Sigma (or other company) page for assay or assay reagents (substrates)**

http://www.sciencedirect.com/science/article/pii/S0968089606008443
 * -- link to paper that contains assay information**

3ZU5
 * Structure Available (PDB or Homology model)**
 * -- PDB # or closest PDB entry if using homology model:**

100%
 * -- Query Coverage (if not direct match):**

99%
 * -- Max Ident (if not direct match):**


 * Druggable Target (see Databases for this):**
 * Current Inhibitors:**


 * Expression Information (has it been expressed in bacterial cells):**
 * Purification Method:**
 * Image of protein (PyMol or etc):**


 * Amino Acid Sequence:**

MIIKPRVRGFICVTAHPTGCEANVKKQIDYVTTEGPIANGPKRVLVIGASTGYGLAARIT AAFGCGADTLGVFFERPGEEGKPGTSGWYNSAAFHKFAAQKGLYAKSINGDAFSDEIKQL TIDAIKQDLGQVDQVIYSLASPRRTHPKTGEVFNSALKPIGNAVNLRGLDTDKEVIKESV LQPATQSEIDSTVAVMGGEDWQMWIDALLDAGVLAEGAQTTAFTYLGEKITHDIYWNGSI GAAKKDLDQKVLAIRESLAAHGGGDARVSVLKAVVTQASSAIPMMPLYLSLLFKVMKEKG THEGCIEQVYSLYKDSLCGDSPHMDQEGRLRADYKELDPEVQNQVQQLWDQVTNDNIYQL TDFVGYKSEFLNLFGFGIDGVDYDADVNPDVKIPNLIQG

399
 * length of your protein in Amino Acids**

43346.0
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website**

code Ext. coefficient   48610 Abs 0.1% (=1 g/l)  1.121, assuming all pairs of Cys residues form cystines code code Ext. coefficient   48360 Abs 0.1% (=1 g/l)  1.116, assuming all Cys residues are reduced code
 * Molar Extinction coefficient of your protein at 280 nm wavelength:**


 * CDS Gene Sequence:**

ATGATTATCAAGCCGCGTGTACGTGGTTTTATCTGTGTTACTGCGCATCCAACGGGTTGCGAGGCTAACGTGAAAAAACAGATCGACTACGTTACCACCGAAGGTCCGATTGCGAACGGTCCGAAACGTGTTCTGGTTATTGGTGCGTCTACCGGTTACGGTCTGGCAGCCCGTATCACGGCGGCTTTTGGTTGCGGCGCAGATACCCTGGGCGTTTTCTTCGAACGTCCTGGCGAAGAAGGCAAACCGGGTACGTCTGGTTGGTACAATTCTGCGGCGTTCCACAAATTCGCTGCGCAAAAAGGTCTGTACGCGAAATCTATCAACGGTGATGCGTTCTCTGACGAAATTAAGCAGCTCACCATTGACGCGATCAAACAGGACCTCGGTCAGGTTGACCAAGTTATTTACTCTCTCGCGTCTCCACGTCGTACCCACCCGAAGACCGGTGAAGTTTTCAACTCTGCGCTGAAACCTATTGGTAATGCTGTAAATCTGCGTGGCCTGGACACCGACAAAGAAGTTATCAAAGAATCTGTCCTGCAGCCGGCGACCCAGTCTGAAATCGACTCTACCGTTGCGGTCATGGGCGGTGAGGATTGGCAAATGTGGATCGATGCCCTCCTGGACGCGGGTGTTCTCGCGGAAGGTGCCCAAACCACCGCGTTCACCTACCTGGGTGAGAAAATCACGCACGATATCTATTGGAACGGTTCTATCGGTGCGGCGAAAAAAGACCTGGACCAGAAAGTTCTGGCGATCCGTGAATCTCTGGCGGCTCACGGTGGCGGCGATGCTCGTGTTTCTGTTCTGAAAGCGGTTGTTACCCAGGCGTCTTCTGCGATCCCTATGATGCCGCTCTACCTGTCTCTGCTGTTCAAAGTTATGAAAGAGAAAGGTACGCATGAAGGTTGCATCGAGCAGGTCTACTCCCTCTACAAAGATTCTCTGTGCGGTGATTCTCCGCACATGGACCAAGAAGGTCGTCTCCGTGCGGACTACAAGGAGCTCGACCCTGAAGTCCAGAATCAGGTTCAGCAGCTGTGGGACCAGGTGACCAACGACAACATCTACCAACTGACGGATTTCGTGGGTTACAAATCTGAATTCCTCAACCTCTTCGGTTTCGGTATCGACGGTGTTGACTACGACGCGGATGTTAACCCGGACGTCAAAATCCCGAATCTGATCCAGGGTTAA


 * GC% Content for gene:**

52.5%


 * CDS Gene Sequence (codon optimized) - after having done Primer Design:**

__ATGATTATCAAGCCGCGTGT__ACGTGGTTTTATCTGTGTTACTGCGCATCCAACGGGTTGCGAGGCTAACGTGAAAAAACAGATCGACTACGTTACCACCGAAGGTCCGATTGCGAACGGTCCGAAACGTGTTCTGGTTATTGGTGCGTCTACCGGTTACGGTCTGGCAGCCCGTATCACGGCGGCTTTTGGTTGCGGCGCAGATACCCTGGGCGTTTTCTTCGAACGTCCTGGCGAAGAAGGCAAACCGGGTACGTCTGGTTGGTACAATTCTGCGGCGTTCCACAAATTCGCTGCGCAAAAAGGTCTGTACGCGAAATCTATCAACGGTGATGCGTTCTCTGACGAAATTAAGCAGCTCACCATTGACGCGATCAAACAGGACCTCGGTCAGGTTGACCAAGTTATTTACTCTCTCGCGTCTCCACGTCGTACCCACCCGAAGACCGGTGAAGTTTTCAACTCTGCGCTGAAACCTATTGGTAATGCTGTAAATCTGCGTGGCCTGGACACCGACAAAGAAGTTATCAAAGAATCTGTCCTGCAGCCGGCGACCCAGTCTGAAATCGACTCTACCGTTGCGGTCATGGGCGGTGAGGATTGGCAAATGTGGATCGATGCCCTCCTGGACGCGGGTGTTCTCGCGGAAGGTGCCCAAACCACCGCGTTCACCTACCTGGGTGAGAAAATCACGCACGATATCTATTGGAACGGTTCTATCGGTGCGGCGAAAAAAGACCTGGACCAGAAAGTTCTGGCGATCCGTGAATCTCTGGCGGCTCACGGTGGCGGCGATGCTCGTGTTTCTGTTCTGAAAGCGGTTGTTACCCAGGCGTCTTCTGCGATCCCTATGATGCCGCTCTACCTGTCTCTGCTGTTCAAAGTTATGAAAGAGAAAGGTACGCATGAAGGTTGCATCGAGCAGGTCTACTCCCTCTACAAAGATTCTCTGTGCGGTGATTCTCCGCACATGGACCAAGAAGGTCGTCTCCGTGCGGACTACAAGGAGCTCGACCCTGAAGTCCAGAATCAGGTTCAGCAGCTGTGGGACCAGGTGACCAACGACAACATCTACCAACTGACGGATTTCGTGGGTTACAAATCTGAATTCCTCAACCTCTTCGGTTTCGGTATCGACGGTGTTGACTACGACGCGGATGTTAACCCGGACGTCAAAATCCCG__AATCTGATCCAGGGTTAA__


 * GC% Content for gene (codon optimized):**

52.5%


 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**


 * Primer design results for 'tail' primers (this is just 2 sequences):**

Upstream 5’- TACTTCCAATCC ATG __ATTATCAAGCCGCGT__ – 3’

Downstream 5’ – TATCCACCTTTACTG TTA __ACCCTGGATCAGATT__ – 3’

[|Primer Design Results]

http://wwww.patricbrc.org/portal/portal/patric/Feature?cType=feature&cId=14081532 http://blast.ncbi.nlm.nih.gov/Blast.cgi http://www.rcsb.org/pdb/explore/explore.do?structureId=3ZU5

LOGFileResults Primer Design https://docs.google.com/open?id=0B4O2KqKh2q_-R1o0OEkwUEczWFU code >lcl|45029 unnamed protein product Length=399

Score = 824 bits (2128),  Expect = 0.0, Method: Compositional matrix adjust. Identities = 398/399 (99%), Positives = 399/399 (100%), Gaps = 0/399 (0%)

Query 7    MIIKPRVRGFICVTAHPTGCEANVKKQIDYVTTEGPIANGPKRVLVIGASTGYGLAARIT  66 MIIKPRVRGFICVTAHPTGCEANVKKQIDYVTTEGPIANGPKRVLVIGASTGYGLAARIT Sbjct 1    MIIKPRVRGFICVTAHPTGCEANVKKQIDYVTTEGPIANGPKRVLVIGASTGYGLAARIT  60

Query 67   AAFGCGADTLGVFFERPGEEGKPGTSGWYNSAAFHKFAAQKGLYAKSINGDAFSDEIKQL  126 AAFGCGADTLGVFFERPGEEGKPGTSGWYNSAAFHKFAAQKGLYAKSINGDAFSDEIKQL Sbjct 61   AAFGCGADTLGVFFERPGEEGKPGTSGWYNSAAFHKFAAQKGLYAKSINGDAFSDEIKQL  120

Query 127  TIDAIKQDLGQVDQVIYSLASPRRTHPKTGEVFNSALKPIGNAVNLRGLDTDKEVIKESV  186 TIDAIKQDLGQVDQVIYSLASPRRTHPKTGEVFNSALKPIGNAVNLRGLDTDKEVIKESV Sbjct 121  TIDAIKQDLGQVDQVIYSLASPRRTHPKTGEVFNSALKPIGNAVNLRGLDTDKEVIKESV  180

Query 187  LQPATQSEIDSTVAVMGGEDWQMWIDALLDAGVLAEGAQTTAFTYLGEKITHDIYWNGSI  246 LQPATQSEIDSTVAVMGGEDWQMWIDALLDAGVLAEGAQTTAFTYLGEKITHDIYWNGSI Sbjct 181  LQPATQSEIDSTVAVMGGEDWQMWIDALLDAGVLAEGAQTTAFTYLGEKITHDIYWNGSI  240

Query 247  GAAKKDLDQKVLAIRESLAAHGGGDARVSVLKAVVSQASSAIPMMPLYLSLLFKVMKEKG  306 GAAKKDLDQKVLAIRESLAAHGGGDARVSVLKAVV+QASSAIPMMPLYLSLLFKVMKEKG Sbjct 241  GAAKKDLDQKVLAIRESLAAHGGGDARVSVLKAVVTQASSAIPMMPLYLSLLFKVMKEKG  300

Query 307  THEGCIEQVYSLYKDSLCGDSPHMDQEGRLRADYKELDPEVQNQVQQLWDQVTNDNIYQL  366 THEGCIEQVYSLYKDSLCGDSPHMDQEGRLRADYKELDPEVQNQVQQLWDQVTNDNIYQL Sbjct 301  THEGCIEQVYSLYKDSLCGDSPHMDQEGRLRADYKELDPEVQNQVQQLWDQVTNDNIYQL  360

Query 367  TDFVGYKSEFLNLFGFGIDGVDYDADVNPDVKIPNLIQG  405 TDFVGYKSEFLNLFGFGIDGVDYDADVNPDVKIPNLIQG Sbjct 361  TDFVGYKSEFLNLFGFGIDGVDYDADVNPDVKIPNLIQG  399 code