TargetSummer17+-+AMP+deaminase,+putative+(P.+falciparum)


 * TargetSummer17 – AMP deaminase, putative ( // P. falciparum // ) **


 * Target (protein/gene name): AMP deaminase, putative **

**NCBI #** ** : ** S59996

**EC #** ** : ** 3.5.4.6

**Protein ID (NP or XP #) or Wolbachia#:** MAL13P1.146

** Organism (including strain): ** // malaria parasite //// Plasmodium falciparum //

__ **Etiologic Risk Group (see link below): ??** __

**Disease Information** **(sort of like the Intro to your Mini****Research Write** **up):**

Plasmodium falciparum is a protozoan parasite that causes malaria. P. falciparum is transmitted by mosquitoes.

**Link to TDR Targets page (if present):** http://tdrtargets.org/targets/view?gene_id=54


 * Link to Gene Database page NCBI: **https://www.ncbi.nlm.nih.gov/protein/S59996

**Essentiality of this protein:**

Gene/Ortholog: Tb11.02.1340 (OG4_10329); Phenotype: significant loss of fitness in bloodstream forms (6 days); Source study: alsford

__** Is it a monomer or multimer as biological unit: ** ?? __


 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **

The druggable index for AMP deaminase, putative on 0-1 scale is a 0.8. This target has been proved to be druggable in a number of other organisms. https://www.ncbi.nlm.nih.gov/pubmed/10780906 https://www.ncbi.nlm.nih.gov/pubmed/10780906

** Link to BRENDA EC# page: ** http://www.brenda-enzymes.org/enzyme.php?ecno=3.5.4.6

** Enzyme Assay ** ** information: ** http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/learning-center/assay-library/ec-number-iii.html

**Structure (PDB or Homology model__)__** No structure available in the PDB. No known Homology model. __ **PDB #:** __

__ **Image of protein (PyMol with features delineated and shown separately):** __

code ACRPPLQLQELFTRSLAESELRSAPYEFPEESPIEQLEERRQRLERQISQDVKLEPDILLRAKQDFLKTD SDSDLQLYKEQGEGQGDRSLRERDVLEREFQRVTISGEEKCGVPFTDLLDAAKSVVRALFIREKYMALSL QSFCPTTRRYLQQLAEKPLETRTYEQGPDTPVSADAPVHPPALEQHPYEHCEPSTMPGDLGLGLRMVRGV VHVYTRREPDEHCSEVELPYPDLQEFVADVNVLMALIINGPIKSFCYRRLQYLSSKFQMHVLLNEMKELA AQKKVPHRDFYNIRKVDTHIHASSCMNQKHLLRFIKRAMKRHLEEIVHVEQGREQTLREVFESMNLTAYD LSVDTLDVHADRNTFHRFDKFNAKYNPIGESVLREIFIKTDNRVSGKYFAHIIKEVMSDLEESKYQNAEL RLSIYGRSRDEWDKLARWAVMHRVHSPNVRWLVQVPRLFDVYRTKGQLANFQEMLENIFLPLFEATVHPA SHPELHLFLEHVDGFDSVDDESKPENHVFNLESPLPEAWVEEDNPPYAYYLYYTFANMAMLNHLRRQRGF HTFVLRPHCGEAGPIHHLVSAFMLAENISHGLLLRKAPVLQYLYY code
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **


 * Length of your protein in Amino Acids: ** 672 amino acids.

**Molecular Weight** **of your protein in kiloDaltons:** 79309 DA

**Molar** **Extinction coefficient** ** of your protein at 280 nm wavelength: ** 59750 mol^-1cm-1


 * TMpred graph Image: **http://embnet.vital-it.ch/cgi-bin/TMPRED_form_parser



__** *CDS ** ** Gene Sequence ** ** (paste as text only): **__

Do Not Need this info for Spring (but still copy these lines to your Target page for now)

***GC% Content for gene:** **output**

***GC% Content for gene (codon optimized):**

**Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):** **(** ** link to DNA Works output ** ** text file ** ** - ** that should be saved in your Google Docs folder after you did the primer design protocol) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.

**Primer design results for 'tail' primers (this is just 2 sequences):**