TargetF15+-+Serine+Threonine+Protein+Phosphatase+1+(Plasmodium+falciparum)

ABSA ranking - 2 //Plasmodium falciparum// is the predominant causative agent of the disease malaria. Malaria is found predominantly in tropical and subtropical regions but can pose a risk to North America, Europe, and northern Asia [[|1]]. Malaria’s spread is mediated by infected //Anopheles// mosquitos transmitting //P. falciparum// during blood meals. From here, the parasite multiplies in the human liver and infects red blood cells. Through blood meals on infected humans by uninfected //Anopheles// mosquitos, //P. falciparum// spreads as the cycle of disease continues [[|1]]. //P. falciparum // infections often progress to fatal forms of cerebral malaria, acute renal failure or acute respiratory distress syndrome [1]. http://www.ncbi.nlm.nih.gov/gene/813836 http://plasmodb.org/plasmo/showRecord.do?name=GeneRecordClasses.GeneRecordClass&source_id=PF3D7_1414400&project_id=PlasmoDB Is it a monomer or multimer as biological unit ** ? (make prediction at ** @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): Monomeric The links followed below are tested on //P. falciparum// Okadaic acid has articles linking to no inhibition and also a link to one with inhibition: [|Link to No Inhibition] [|Link to Inhibition IC50: 4 nM] Trifluoperazine [|Inhibition of PfPP2B]
 * Target: **Serine Threonine Protein Phosphatase 1 (PP1)
 * *NCBI Gene # or RefSeq#: **811723 (PP1)
 * *Protein ID (NP or XP #) or Wolbachia#: ** Pf3D7_1414400
 * Organism (including strain): **Plasmodium falciparum
 * Etiologic Risk Group (see link below): ** Group B
 * Disease Information : **
 * Link to TDR Targets page: **[]
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) **
 * Essentiality of this protein: **
 * Complex of proteins?: ** 1 amino acid chain with 7 ligands. The structure do not form a complex.
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **


 * *EC#: ** 3.1.3.16
 * Link to BRENDA EC# page: **[|3.1.3.16]

http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/learning-center/assay-library/ec-number-iii.html
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **Spectrophotometric pNPP assay
 * -- link to Sigma (or other company ) page for assay (see Sigma links below) **
 * N/A ** Link is offline as of 10/18 12:40 A.M. :


 * -- -or link (or citation) to paper that contains assay information **
 * -- links to assay reagents (substrates) pages. **
 * --- List cost and quantity of substrate reagents, supplier, and catalog # **

Closest PDB entry if using homology model: 1JK7 -- For Homology Model option: Query Coverage: 98% Max % Identities: 86% % Positives: 94% Template Model MALEIDIDNVISKLIEVRGTRPGKNVNLTENEIKILCLSSREIFLNQPILLELEAPIKIC GDIHGQFYDLLRLFEYGGFPPDANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLR GNHECASINRIYGFYDECKRRYSVKLWKTFIDCFNCLPVAAIIDEKIFCMHGGLSPELNN MEQIRKITRPTDVPDNGLLCDLLWSDPEKEINGWGENDRGVSFTFGQDVVHNFLRKHELD LICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPVEKK KAAN TACTTCCAATCCATGGCGCTGGAAATC TATTTCCAAT CAATGGCACT TGAAATT 29.6%
 * Structure (PDB or Homology model) **
 * Molprobity Comparison**
 * Current Inhibitors: ** cyclosporine A, okadaic acid, trifluoperazine
 * Expression Information (has it been expressed in bacterial cells): ** It has been expressed in E. coli M15 cells.
 * Purification Method : ** N- terminal affinity tag added to polypeptide. Nickel - chelation chromatography is used for purification of the His tag.
 * Image of protein (PyMol with features delineated and shown separately): **
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **
 * *length of your protein in Amino Acids: **304 (323 residues in 1JK7)
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website: **34.9042 kDa
 * Molar Extinction coefficient of your protein at 280 nm wavelength: **33640 (assuming all Cys residues form cystines); 32890 (assuming a;; Cys residues are reduced)
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
 * *CDS Gene Sequence (paste as text only): **
 * *GC% Content for gene: 48.1% **
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **
 * *GC% Content for gene (codon optimized): **


 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( [|link to DNA Works output] [|text file]) **

5' TACTTCCAATCCATGGCGCTGGAAATC **3' **
 * Primer design results for 'tail' primers (this is just 2 sequences): **
 * Forward **



5' TATCCACCTTTACTGTTAGTTCGC 3'
 * Reverse**