Target-+Dihydrofolate+Reductase+M.+tuberculosis

​http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.5.1.3&Suchword=&organism[]=Mycobacterium+tuberculosis&show_tm=0
 * Target (protein/gene name):** Dihydrofolate Reductase
 * NCBI Gene # or RefSeq#:** 14966432
 * Protein ID (NP or XP #) or Wolbachia#:** YP_007611485.1
 * Organism (including strain):** //M. tuberculosis//
 * Etiologic Risk Group (see link below):** RG3
 * Background/Disease Information (sort of like the Intro to your Mini Research Write up):** //Mycobacterium tuberculosis// is a pathogenic bacteria that is the causative agent of tuberculosis. The bacteria has an unusual, waxy coating that prevents it from being effectively tested with Gram staining and instead acid-fast detection is used. The pathogen is highly aerobic and primarily infects the lungs of mammals. Tuberculosis is an infectious disease that in many cases is legal. It is spread through the air by coughing, sneezing, or transmission of respiratory fluids from an infected person. Symptoms include chronic cough, blood-tinged sputum, fever, night sweats, and weight loss. Diagnosis of TB is dependent on radiology, usually a chest X-ray. Tuberculosis is much more active in the developing world with about 80% of the population in many Asian and African countries testing positive for tuberculosis, while only about 5-10% test positive in the U.S. population. The emergence of multiple drug-resistant tuberculosis (MDR-TB) is causing a big push for the development of new drugs for this infectious disease.
 * Essentiality of this protein:** Essential
 * Complex of proteins?:** No
 * Druggable Target:** Yes
 * *EC#: ** 1.5.1.3
 * Link to BRENDA EC# page:**



--[] --http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Enzyme_Assay/dihydrofolatereductase.pdf potassium phosphate buffer : 100g for $19.90 ß-NADPH: ........................... 15VL for $345.00 DHFA: ..................................10 mg for $71.60 BSA:........................................ 5g for $159.00 DHFR Enzyme solution:.. .25 units for $241.50
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**
 * --**Reagents: (Sigma)

-- 1DG8
 * Structure Available (PDB or Homology model)**

--4,5-dideoxy-5-(2,6-diamino-5-phenylpyrimidin-4-yl)-D-erythro-pentitol --6-([(2,5-dipropoxyphenyl)amino]methyl)-5-methylpyrido[2,3-d]pyrimidine-2,4-diamine --6-([(2-butoxy-5-ethoxyoxyphenyl)amino]methyl)-5-methylpyrido[2,3-d]pyrimidine-2,4-diamine --6-([(2-butoxy-5-propoxyphenyl)amino]methyl)-5-methylpyrido[2,3-d]pyrimidine-2,4-diamine --6-([(2-ethoxy-5-butoxyphenyl)amino]methyl)-5-methylpyrido[2,3-d]pyrimidine-2,4-diamine --Isoniazid --methotrexate --Pyrimethamine --trimethoprim --tripeptide inhibitor WYY
 * Current Inhibitors:**
 * --**3-([(2,4-diamino-5-methylpyrido[2,3-d]pyrimidin-6-yl)methyl]amino)-4-(2,5-dimethoxyethoxy)phenol

MVGLIWAQATSGVIGRGGDIPWRLPEDQAHFREITMGHTIVMGRRTWDSLPAKVRPLPGRRNVVLSRQADFMASGAEVVG SLEEALTSPETWVIGGGQVYALALPYATRCEVTEVDIGLPREAGDALAPVLDETWRGETGEWRFSRSGLRYRLYSYHRS GTGACGATGGTGGGGCTGATCTGGGCTCAAGCGACATCGGGTGTCATCGGCCGCGGCGGCGACATCCCCT GGCGCTTGCCCGAGGACCAGGCGCATTTCCGGGAGATCACCATGGGGCACACGATCGTGATGGGCCGGCG CACATGGGATTCGCTGCCGGCTAAAGTCCGGCCGCTGCCCGGCCGGCGAAATGTCGTACTGAGCCGCCAA GCTGACTTTATGGCCAGCGGGGCTGAGGTTGTCGGTTCACTCGAGGAGGCGCTGACCAGCCCGGAGACGT GGGTGATCGGAGGCGGACAAGTCTATGCGCTGGCGCTGCCGTACGCGACCAGATGTGAGGTTACCGAGGT CGACATCGGCCTGCCGCGCGAAGCCGGTGACGCGCTGGCCCCCGTGCTGGACGAGACATGGCGGGGCGAG ACGGGGGAGTGGCGCTTCAGCCGGTCCGGGTTGCGGTACCGGTTGTACAGCTACCACCGCTCATGA
 * Expression Information (has it been expressed in bacterial cells):** Yes in E.coli cells
 * Purification Method:** metal-chelate affinity chromatography [1]
 * Image of protein (PyMol with features delineated and shown separately):**
 * Amino Acid Sequence:**
 * length of your protein in Amino Acids:** 159
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website:** 17640.0
 * Molar Extinction coefficient of your protein at 280 nm wavelength:** 40450
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html)
 * CDS Gene Sequence (paste as text only):**
 * *GC% Content for gene: ** 67.08%
 * CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):**
 * GC% Content for gene (codon optimized):**


 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**
 * ( link to DNA Works output text file - **that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences):**

Resources:
[1] Argyrou, Argyrides; Vetting, Matthew W.; Aladegbami, Bola; Blanchard, John S., // Mycobacterium tuberculosis // dihydrofolate reductase is a target for isoniazid. // Nature Stuctural & Molecular Biology, //** 2006 **. 13. 408-13.

See **ProtocolTargetDiscoveryVDS.docx** for more

Etiologic Risk Group Categories (for pathogens): http://www.utexas.edu/research/rsc/ibc/agent_class.html#_Toc7238334


 * Databases of genes/organisms:**

@http://www.niaid.nih.gov/Pages/default.aspx

@http://eupathdb.org/eupathdb/

@https://patricbrc.vbi.vt.edu/portal/portal/patric/Home

@http://www.nmpdr.org/FIG/wiki/view.cgi/Main/EssentialGenes

@http://tubic.tju.edu.cn/deg/

@http://csgid.org/csgid/cake/pages/community_request_gateway

@http://tdrtargets.org/

@http://gsc.jcvi.org/status.shtml


 * Scientific Nomenclature page from Center for Disease Control (gene, protein names and abbreviations)**

@http://wwwnc.cdc.gov/eid/pages/scientific-nomenclature.htm


 * Gene Information:**

NCBI GENE Page: @http://www.ncbi.nlm.nih.gov/gene

BLAST Page: @http://blast.ncbi.nlm.nih.gov/


 * Protein Information:**

NCBI Protein Page: @http://www.ncbi.nlm.nih.gov/protein

Protein Expression Website

Protein Expression Paper:


 * Primer Overlap PCR Articles**






 * Is my target good for Virtual Screening programs?**