Target-+DIHYDROLIPOAMIDE+DEHYDROGENASE+LPD+(LIPOAMIDE+REDUCTASE+(NADH))+(Mycobacterium+tuberculosis)

DIHYDROLIPOAMIDE DEHYDROGENASE LPD (LIPOAMIDE REDUCTASE (NADH)) (LIPOYL DEHYDROGENASE) (DIHYDROLIPOYL DEHYDROGENASE) (DIAPHORASE 75766096 Rv0462 //Mycobacterium tuberculosis// H37Rv
 * Target (protein/gene name):**
 * NCBI Gene # or RefSeq#:**
 * Protein ID (NP or XP #) or Wolbachia#:**
 * Organism (including strain):**

Risk Group 3
 * Etiologic Risk Group (see link below):**

Tuberculosis is a serious infectious disease affecting the lungs, and it can be fatal if untreated. The disease is caused by the bacterium //Mycobacterium tuberculosis,// which is released into the air when an infected person coughs or sneezes, making the disease highly contagious. Symptoms of tuberculosis include chronic cough, chest pain, coughing up blood or septum, night sweats, fever, and weight loss. People can become infected with the //Mycobacterium tuberculosis// without developing the disease because it can remain latent in the body. Certain individuals are more susceptible to tuberculosis, such as people infected with HIV. Because of the growing problem of bacterial resistance, it is important that new methods for treating tuberculosis and other bacterial diseases are developed.
 * Background/ Disease Information :**

http://www.tdrtargets.org/targets/view?gene_id=6322
 * Link to TDR Targets page (if present):**

http://www.ncbi.nlm.nih.gov/protein/?term=2A8X
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.)**


 * Essentiality of this protein:**
 * Gene/Ortholog:** Tb11.01.8470 (OG4_10422); **Phenotype:** significant gain of fitness in bloodstream forms (3 days); **Source study:** alsford
 * Gene/Ortholog:** Tb11.01.8470 (OG4_10422); **Phenotype:** significant gain of fitness in bloodstream forms (6 days); **Source study:** alsford
 * Gene/Ortholog:** Tb11.01.8470 (OG4_10422); **Phenotype:** significant loss of fitness in procyclic forms; **Source study:** alsford
 * Gene/Ortholog:** Tb11.01.8470 (OG4_10422); **Phenotype:** significant loss of fitness in differentiation of procyclic to bloodstream forms; **Source study:** alsford

No
 * Complex of proteins?:**

Druggabilty: 0.5
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):**

1.8.1.4
 * *EC#: **

http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.8.1.4
 * Link to BRENDA EC# page:**



[]
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**
 * -- link to Sigma (or other company ) page for assay (see Sigma links below)**

A. 50 mM Sodium Phosphate Buffer, pH 6.5 at 25°C [|Sigma Prod. No. S-0751] B. 28 mM DL-Thioctic Acid Amide Solution (DL-Thio) [|Sigma Prod. No. T-5875] C. 7.0 mM ß-Nicotinamide Adenine Dinucleotide, Reduced Form Solution (ß-NADH) [|Sigma Prod.No. N-8129] [|Sigma Stock No. 340-110] D. 20 mM ß-Nicotinamide Adenine Dinucleotide, Oxidized Form Solution (ß-NAD) [|Sigma Stock No. 260-150] [|Sigma Prod. No. N-7004] E. 300 mM Ethylenediaminetetraacetic Acid with 2.0% (w/v) Albumin Solution, pH 7.0 at 25°C (EDTA) [|Sigma Stock No. ED4S] [|Sigma Prod. No. A-4503] F. Lipoamide Dehydrogenase Enzyme Solution
 * -- links to assay reagents (substrates) pages.**
 * --- List cost and quantity of substrate reagents, supplier, and catalog #**

2A8X 3II4
 * Structure Available (PDB or Homology model)**

[|CHEMBL405110]
 * Current Inhibitors:**

The protein has been expressed in E. coli. http://www.ncbi.nlm.nih.gov/pubmed/11560483 http://pubs.acs.org/doi/pdf/10.1021/bi010575o
 * Expression Information (has it been expressed in bacterial cells):**
 * Purification Method :**

mthydvvvlg agpggyvaai raaqlglsta ivepkywggv clnvgcipsk allrnaelvh iftkdakafg isgevtfdyg iaydrsrkva egrvagvhfl mkknkiteih gygtfadant llvdlndggt esvtfdnaii atgsstrlvp gtslsanvvt yeeqilsrel pksiiiagag aigmefgyvl knygvdvtiv eflpralpne dadvskeiek qfkklgvtil tatkvesiad ggsqvtvtvt kdgvaqelka ekvlqaigfa pnvegygldk agvaltdrka igvddymrtn vghiyaigdv ngllqlahva eaqgvvaaet iagaetltlg dhrmlpratf cqpnvasfgl teqqarnegy dvvvakfpft anakahgvgd psgfvklvad akhgellggh lvghdvaell peltlaqrwd ltaselarnv hthptmseal qecfhglvgh minf
 * Image of protein (PyMol with features delineated and shown separately):**
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):**


 * length of your protein in Amino Acids:** 465 aa

49239.1 g/mol
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website**

Ext. coefficient 30620 Abs 0.1% (=1 g/l) 0.622, assuming all pairs of Cys residues form cystines
 * Molar Extinction coefficient of your protein at 280 nm wavelength:**

Ext. coefficient 30370 Abs 0.1% (=1 g/l) 0.617, assuming all Cys residues are reduced


 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.

gtgacccact atgacgtcgt cgttctcgga gccggtcccg gcgggtatgt cgcggcgatt cgcgccgcac agctcggcct gagcactgca atcgtcgaac ccaagtactg gggcggagta tgcctcaatg tcggctgtat cccatccaag gcgctgttgc gcaacgccga actggtccac atcttcacca aggacgccaa agcatttggc atcagcggcg aggtgacctt cgactacggc atcgcctatg accgcagccg aaaggtagcc gagggcaggg tggccggtgt gcacttcctg atgaagaaga acaagatcac cgagatccac gggtacggca catttgccga cgccaacacg ttgttggttg atctcaacga cggcggtaca gaatcggtca cgttcgacaa cgccatcatc gcgaccggca gtagcacccg gctggttccc ggcacctcac tgtcggccaa cgtagtcacc tacgaggaac agatcctgtc ccgagagctg ccgaaatcga tcattattgc cggagctggt gccattggca tggagttcgg ctacgtgctg aagaactacg gcgttgacgt gaccatcgtg gaattccttc cgcgggcgct gcccaacgag gacgccgatg tgtccaagga gatcgagaag cagttcaaaa agctgggtgt cacgatcctg accgccacga aggtcgagtc catcgccgat ggcgggtcgc aggtcaccgt gaccgtcacc aaggacggcg tggcgcaaga gcttaaggcg gaaaaggtgt tgcaggccat cggatttgcg cccaacgtcg aagggtacgg gctggacaag gcaggcgtcg cgctgaccga ccgcaaggct atcggtgtcg acgactacat gcgtaccaac gtgggccaca tctacgctat cggcgatgtc aatggattac tgcagctggc gcacgtcgcc gaggcacaag gcgtggtagc cgccgaaacc attgccggtg cagagacttt gacgctgggc gaccatcgga tgttgccgcg cgcgacgttc tgtcagccaa acgttgccag cttcgggctc accgagcagc aagcccgcaa cgaaggttac gacgtggtgg tggccaagtt cccgttcacg gccaacgcca aggcgcacgg cgtgggtgac cccagtgggt tcgtcaagct ggtggccgac gccaagcacg gcgagctact gggtgggcac ctggtcggcc acgacgtggc cgagctgctg ccggagctca cgctggcgca gaggtgggac ctgaccgcca gcgagctggc tcgcaacgtc cacacccacc caacgatgtc tgaggcgctg caggagtgct tccacggcct ggttggccac atgatcaatt tctga
 * CDS Gene Sequence (paste as text only):**


 * GC% Content for gene:**
 * CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):**
 * GC% Content for gene (codon optimized)**