TargetSp15-NucleosideTriphosphateDiphosphohydrolase+(Toxoplasma+gondii)

Protein: NUCLEOSIDE TRIPHOSPATE DIPHOSPHOHYDROLASE 3 Gene: NTP3 Is it a monomer or multimer as biological unit**? (make prediction at** @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): Multimer (4-mer) "Pretreatment of parasites with a monoclonal antibody inhibiting both NTPDases significantly decreased the number of infected cells, suggesting that  one or both isoforms are involved in invasion of the parasites into host cells" [1].
 * Target (protein/gene name):**
 * NCBI Gene # or RefSeq#:** 7901558
 * Protein ID (NP or XP #) or Wolbachia#:** XP_002370463.1
 * Organism (including strain):** //Toxoplasma gondii//
 * Etiologic Risk Group (see link below):** Appendix B-II-C. Risk Group 2 (RG2) - Parasitic Agents
 * / Disease Information (sort of like the Intro to your Mini __Research Write__ up):** //Toxoplasma gondii// infects humans through meat, by ingestion of materials that had made contact with infected cat feces, and from infected pregnant mothers to their fetuses. This protozoan species causes the disease toxoplasmosis, which usually produces mild flu-like symptoms but tends to be more severe in people with weaker immune systems, such as infants and adults with HIV/AIDS.
 * Link to TDR Targets page (if present):** Not Available
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.):** [] (searched through EuPath)
 * Essentiality of this protein: "**Via antisense RNA inhibition, it was demonstrated that NTPDase3 is required for replication of the parasite inside the host cells" [1].
 * Complex of proteins?:** Complexes with AMP, magnesium, and AMPPNP
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):**

[|**http://www.brenda-enzymes.org/enzyme.php?ecno=3.6.1.15#PDB**]
 * *EC#: ** 3.6.1.15
 * Link to BRENDA EC# page:**
 * --** Show screenshot of BRENDA enzyme mechanism schematic


 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**
 * -- link to Sigma (or other company ) page for assay (see Sigma links below)**
 * -- -or link (or citation) to paper that contains assay information:** [] [1]
 * -- links to assay reagents (substrates) pages:** []
 * --- List cost and quantity of substrate reagents, supplier, and catalog #:** $39.20 for 1 mg ATP, supplied by FLAAS SIGMA (SKU: FLAAS-1VL)

-- PDB # or closest PDB entry if using homology model: 4A57 -- For Homology Model option: N/A Show pairwise alignment of your BLASTP search in NCBI against the PDB Query Coverage: Max % Identities: % Positives Chain used for homology:
 * Structure (PDB or Homology model)**

MTDSSSLRGVDADTEKRINVGKKHLQTLRNLETRCHDSLQALVVIDAGSSSTRTNVFLAKTRSCPNKGRSIDPDSIQLIG
 * Current Inhibitors:** Monoclonal antibodies
 * Expression Information (has it been expressed in bacterial cells):** //Escherichia coli // Rosetta pLysS [1]
 * Purification Method :** Exclusion chromatography using FPLC
 * Image of protein (PyMol with features delineated and shown separately): [[image:target.png width="800" height="475"]]**
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):**

AGKRFAGLRVVLEEWLDTYAGKDWESRPVDARLLFQYVPQMHEGAKKLMQLLEEDTVAILDSQLNEKQKVQVKALGIPVM

LCSTAGVRDFHEWYRDALFVLLRHLINNPSPAHGYKFFTNPFWTRPITGAEEGLFAFITLNHLSRRLGEDPARCMIDEYG

VKQCRNDLAGVVEVGGASAQIVFPLQEGTVLPSSVRAVNLQRERLLPERYPSADVVSVSFMQLGMASSAGLFLKELCSND

EFLQGGICSNPCLFKGFQQSCSAGEVEVRPDGSASVNEDVRKNRLKPLATYCSVNNPEISFKVTNEMQCRENSIDPTKPL

AERMKIENCSIIKGTGNFDKCVSQVESILVAPKLPLPANIEAASSGFESVDQVFRFASSTAPMIVTGGGMLAAINTLKDH

RLLRSDFSGDVEELAEAAREFCSSEVIIRTDGPVIQLPNARGEQKLNSLNFDLCKTMALTVSLLRHMAAGENQPSFIKWE

KSIAGPDGKPLADLGWQVGVILHHVLFTEEWGRNAYEAGYSHNLEHHHHHH CCACCTGTCCAGACGGCTAGGCGAAGACCCAGCTAGGTGCATGATTGATGAATACGGGGT
 * *length of your protein in Amino Acids: ** 611 amino acids
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website:** 67.574 kilodaltons
 * Molar Extinction coefficient of your protein at 280 nm wavelength:** 52785 L/mol/cm (51910 L/mol/cm with Cys residues reduced)
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it. [[image:Graph..PNG]]
 * CDS Gene Sequence (paste as text only):**

GAAGCATTGCCGCAATGACCTTGCTGGCGTGGTCGAGGTTGGGGGTGCTTCTGCTCAAAT

TGTCTTTCCACTACAGGAAGGTACCGTCCTGCCCTCTTCGGTCCGTGCCGTCAACCTGCA

GCGCGAACGCCTTCTCCCAGAACGTTATCCAAGTGCCGACGTCGTGTCAGTTTCGTTTAT

GCAGCTGGGTATGGCCAGTTCCGCAGGACTGTTTCTCAAGGAGCTGTGTAGTAACGATGA

GTTTTTGCAAGGCGGAATTTGCTCCAACCCGTGTTTATTTAAGGGCTTTCAGCAGAGTTG

TTCCGCGGGCGAAGTCGAGGTCCGTCCTGACGGATCCGCATCTGTGAACGAAGATGTGAG

AAAGAACAGGCTGAAGCCGCTTGCAACGTACTGCTCAGTACATAACCCGGAAATCAGCTT

CAAGGTAACCAACGAAATGCAGTGCCGGGAAAACTCGATTGATCCAACGAAGCCACTCGC

GGAGAGAATGAAGATCGAGAACTGCAGTATAATCGAAGGAACCGGAAACTTTGACAAATG

CGTGAGCCAGGTAGAAAGCATTCTCGTCGCTCCCAAGTTACCTCTACCAGCCAACATTGA

AGCTGCGTCCTCGGGCTTCGAATCCGTCGACCAAGTCTTCAGGTTTGCCTCCTCGACAGC

ACCAATGTTTATAACGGGACGAGAGATGCTTGCGTCTATAGACACACTCAAAGATCACCG

TCTTCTTCGGAGCGACTTCAGCGGTGACGTAGAAGAACTGGCGGAAGCGGCGCGTGAATT

CTGTTCTTCTGAAGTGATTATCCGTACAGATGGCCCGGTCATCCAGTTGCCAAACGCGCG

AGGGGAACAGAAGCTGAATAGCCTAAACTTCGACTTGTGCAAGACGATGGCGCTGACTGT

GTCTCTGCTTAGGCACATGGCGGCAGGAGAAAATCAACCGTCGTTCATTAAATGGGAAAA

AAGCATTGCAGGACCTGATGGGAAACCACTTGCTGACCTTGGGTGGCAAGTAGGAGTCAT

TCTTCACCACGTCCTCTTCACTGAAGAATGGGGACGTAACGCTTATGAGGCGGGATATTC

TCACAATCTGTGAAATGTCGATTGATGGTGTCGCCTATTATCGGAGAAGATCGTGCTTGC

TACACCAGTGTCTTTCATACTCCTGTGTACTATAGTTCAGATGAGTCGAGGGCTTGCGGA

GAGGTAGTTCTCTAGTGGTGATACGCCGGGGATCTGACCTTTTTGTTGTCGGCTATCGTG

AGACCTGTCTTCCGCGAGCAAGCGCCTTACGTTGTCCTTCGAAGCGCTCCTCGATGGAGC

AAATTCATGCGGGATTTATACCAATAGGGCCGACTCGGAAGAAGATCGGACACCCATGTT

TTTACGCTGCACTTGCGATAACGTAACAACCCGGTAGGCTGCTTATCTCAACTGTTTTAT

TACAGGTTACCGTGTTCGTCTACAGTCCCCAGAAACGCGATGCGTTCCTCTCATCGTGCT

CGTCTCAAGACGACCATCACAAGCACGTGCGCGCTCTAATGTGGTGACAATGTGACGCCG

CTCAATTCTCGCTTATGCTCTGCTCGTTTGTATTGTGTGTTGAAACTGAGCTGCGAACAA

CATTTGATGGCTCCGTGCATTAGCCGGCGCCTCAAGGATTCCTGTGTGATGGACGCCAGT

GACACGCCGACGTCTGTTTTTTGCATGCTCGGCCGTTCGTCCGGTTAGAACTGACAGCAT

GCCTGGTAACAATATAATGGCTGGAAGCGTTGAAACGGTCCTCACGGACTTCACGCACGG

TGTCGCGCCAGAATTGCGTTTCTGATTTCAGCAGCGTTTTTGCACACTTGCAGCGGCAAG

AAGCAACTCAGAAAAGGCTGGTCAATCTATGTAGGTTAGGCACACAGCCGCATCACTGTC

CTTTACATCATTAATTACATGTCTTGTCCCATCTTTTATCATATTCAATCTATATAGGGC

AGCATGAGCAGAGAAGACGCATAGGAGTCTGTCTTTCCGACGGTGTAAGAGGAATAATGG

TGTAAAGTCGTTCTTTTCTACCCGACCAGTGTTACCTCGTTATCTAAAACTGAGTCCTGA

AAAGGCCCACATTCATCGACAAGAAAGGTCCCTGGCCATCAAACAGCAAAGGAGGGAACA

AAGAACGGGGACGCACAGTTCTACAGAGAGGGAATCGAATGCTTTTTTACTTTCGAGTTG

CGGCAGTGAGGACAATGCTTGTCCCGACTGTGCATGCAGACCGTGGCGCCACACTAGTGT

AGGCCTCAGCGGCTGACGCACAAGATCCATTTACCACAGAAACGGAGGTGCTCGAGACCA

GAATGACTTCTCTTGCGGATGTCGTTGGACGATGGAAATCGTGAGGAAGGGGAGGAAACG

AGAGACGCGAAGAGGGCGCAACCCGATTTCGGAGAGGACCGGAAGTGCGTGCCGACCACC

AGGGCGTCTAGCTCAAATGGTAGAGCGCTCGCTTAGCATGCGAGAGGTAATGGGATCGAT

ACCCATGTCGTCCAGTTCTTTTCCGAAATTCCAACTCATTGCGACACTTTTAAACTGCTT

CGATGTTCCGAAAATGTCCGGCCTCTCATCTGATACGTCTTGCTGACACGGGCCGAAATA

GAAAACTCTACTTGCTCTCTGTGGCTGCGTCATATTGCGCCATATCGTGTGTACGGCAAC

GGAGTCAGCCGGATGAACAGCTGCTGTTGACGATTCTGTCCTATTTTCAATTCACGATGA

AAATACTATGAAAAATAGTATGTAGACGTTTTGCTGTAAATGAAGAAATATGCAGAGAAA

CTCGTATCACATGTCGCTGCAAGCCGCCACTAGATCGTCCACAAACCTGTTCCGCTCAGT

TGGCTTTCGTACCATCCGGGCAAGCGCACACAACGGGTCTAGACCGCAGCCTCCTGACTT

CACAAATCTCGAAGTAAGCAGGCTCACTCCTAATTTTCCTCAAGAACCATAAGCATCTAC

CGCCGAGCTGCAACAGTGAGCTCGCCTGTATTCCTCAACCCGCCCTACGACGTCACCCCC

GCGACCGCGGAAATCTGGGTTTTGGATCACGAATCAGTTGACCTGTCCTCCGCTCTAGAG

TCCACTGCATTGAACCGGGAACATTTTTATCGGTTCTACAAGTTTGGAATACGCATGTGT

CTGTTCCCACACCAAGTAACTGCGTCCGTCTGGTCTGTTTCAATTATGTAACTTGGCAAC

GTTGTCGCGATGGCCCTCGCCCGTAATCTGCTCGGATATCACACTGACCATGCCACTTCA

CTTATTTGAGCACATGAAACGGTCGGTGGATGAGCAGTTCAGGACAAACATGCTTGTCGC

TGTCTCAGTCTCTCCAGTTGTTCGGTGTTCTAGGGTGAGTGACGCTGCAGCCAAGCCAGC

CGTAACTCGCGTCTCAAAGGGCACCGGGGCCAGAGTTTGGCAGCCACTTTTTCACACGGC

ACTTCGGCGCACATTTCAGGGTGCACGCGCCAGGCACAGCCGACCGCTTCACGAGGTGTT

AGTGACGCATACCTCGTTCGCGTCTCTCTTCGAATACGGGACACAAACAAATTCCGTTCA

CGACAGATCACCGAAGCGTCATTGGGCACTCATTGTTAACAAACTGCCGTAGCCGATCCG

TTCGCACGTCATACTCGGCCTCGAGCACCGCGCACGTCCTACCCGGAGTCCTCATTGTAT

CCGATCCCGGTACCTCTTTCATTCTACCGCAGCTTCCAGTGTACTTTCCGCGTCACCAAA

GCGGTCAGTAGAACCAGGTTCGAGCTGCTGTTCAGTTTTTTTGACGATCGACGCGTCTTG

CCTTTACTGTTGTGTTCGTACGAATCAGCTGCCGTTTATAGGTAGGCATCATGTGGTTGC

CGGTGTATGTGCCTCTCTTGCTGGTGTTTGGAGTTAGTTTGTCCCTCCCACAAGGAAGCC

TTGGCACAGACTCATCGTCACTCCGGGGAGTGGACGCTGACACTGAAAAGCGGATTAATG

TCGGGAAGAAACACCTGCAGACCCTCCGAAATCTGGAAACTCGGTGCCACGATTCCTTGC

AAGCACTTGTTGTAATCGATGCAGGCAGTAGTTCGACGAGGACGAATGTTTTCTTGGCGA

AAACCCGGTCATGCCCAAATAAAGGCCGTAGCATTGACCCAGACAGTATTCAACTCATCG

GCGCTGGCAAGCGGTTCGCAGGACTTCGTGTAGTTTTAGAAGAGTGGCTGGACACTTACG

CTGGAAAGGACTGGGAGTCG CCACCTGTCCAGACGGCTAGGCGAAGACCCAGCTAGGTGCATGATTGATGAATACGGGGT
 * GC% Content for gene:** 51.36%
 * CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):**

GAAGCATTGCCGCAATGACCTTGCTGGCGTGGTCGAGGTTGGGGGTGCTTCTGCTCAAAT

TGTCTTTCCACTACAGGAAGGTACCGTCCTGCCCTCTTCGGTCCGTGCCGTCAACCTGCA

GCGCGAACGCCTTCTCCCAGAACGTTATCCAAGTGCCGACGTCGTGTCAGTTTCGTTTAT

GCAGCTGGGTATGGCCAGTTCCGCAGGACTGTTTCTCAAGGAGCTGTGTAGTAACGATGA

GTTTTTGCAAGGCGGAATTTGCTCCAACCCGTGTTTATTTAAGGGCTTTCAGCAGAGTTG

TTCCGCGGGCGAAGTCGAGGTCCGTCCTGACGGATCCGCATCTGTGAACGAAGATGTGAG

AAAGAACAGGCTGAAGCCGCTTGCAACGTACTGCTCAGTACATAACCCGGAAATCAGCTT

CAAGGTAACCAACGAAATGCAGTGCCGGGAAAACTCGATTGATCCAACGAAGCCACTCGC

GGAGAGAATGAAGATCGAGAACTGCAGTATAATCGAAGGAACCGGAAACTTTGACAAATG

CGTGAGCCAGGTAGAAAGCATTCTCGTCGCTCCCAAGTTACCTCTACCAGCCAACATTGA

AGCTGCGTCCTCGGGCTTCGAATCCGTCGACCAAGTCTTCAGGTTTGCCTCCTCGACAGC

ACCAATGTTTATAACGGGACGAGAGATGCTTGCGTCTATAGACACACTCAAAGATCACCG

TCTTCTTCGGAGCGACTTCAGCGGTGACGTAGAAGAACTGGCGGAAGCGGCGCGTGAATT

CTGTTCTTCTGAAGTGATTATCCGTACAGATGGCCCGGTCATCCAGTTGCCAAACGCGCG

AGGGGAACAGAAGCTGAATAGCCTAAACTTCGACTTGTGCAAGACGATGGCGCTGACTGT

GTCTCTGCTTAGGCACATGGCGGCAGGAGAAAATCAACCGTCGTTCATTAAATGGGAAAA

AAGCATTGCAGGACCTGATGGGAAACCACTTGCTGACCTTGGGTGGCAAGTAGGAGTCAT

TCTTCACCACGTCCTCTTCACTGAAGAATGGGGACGTAACGCTTATGAGGCGGGATATTC

TCACAATCTGTGAAATGTCGATTGATGGTGTCGCCTATTATCGGAGAAGATCGTGCTTGC

TACACCAGTGTCTTTCATACTCCTGTGTACTATAGTTCAGATGAGTCGAGGGCTTGCGGA

GAGGTAGTTCTCTAGTGGTGATACGCCGGGGATCTGACCTTTTTGTTGTCGGCTATCGTG

AGACCTGTCTTCCGCGAGCAAGCGCCTTACGTTGTCCTTCGAAGCGCTCCTCGATGGAGC

AAATTCATGCGGGATTTATACCAATAGGGCCGACTCGGAAGAAGATCGGACACCCATGTT

TTTACGCTGCACTTGCGATAACGTAACAACCCGGTAGGCTGCTTATCTCAACTGTTTTAT

TACAGGTTACCGTGTTCGTCTACAGTCCCCAGAAACGCGATGCGTTCCTCTCATCGTGCT

CGTCTCAAGACGACCATCACAAGCACGTGCGCGCTCTAATGTGGTGACAATGTGACGCCG

CTCAATTCTCGCTTATGCTCTGCTCGTTTGTATTGTGTGTTGAAACTGAGCTGCGAACAA

CATTTGATGGCTCCGTGCATTAGCCGGCGCCTCAAGGATTCCTGTGTGATGGACGCCAGT

GACACGCCGACGTCTGTTTTTTGCATGCTCGGCCGTTCGTCCGGTTAGAACTGACAGCAT

GCCTGGTAACAATATAATGGCTGGAAGCGTTGAAACGGTCCTCACGGACTTCACGCACGG

TGTCGCGCCAGAATTGCGTTTCTGATTTCAGCAGCGTTTTTGCACACTTGCAGCGGCAAG

AAGCAACTCAGAAAAGGCTGGTCAATCTATGTAGGTTAGGCACACAGCCGCATCACTGTC

CTTTACATCATTAATTACATGTCTTGTCCCATCTTTTATCATATTCAATCTATATAGGGC

AGCATGAGCAGAGAAGACGCATAGGAGTCTGTCTTTCCGACGGTGTAAGAGGAATAATGG

TGTAAAGTCGTTCTTTTCTACCCGACCAGTGTTACCTCGTTATCTAAAACTGAGTCCTGA

AAAGGCCCACATTCATCGACAAGAAAGGTCCCTGGCCATCAAACAGCAAAGGAGGGAACA

AAGAACGGGGACGCACAGTTCTACAGAGAGGGAATCGAATGCTTTTTTACTTTCGAGTTG

CGGCAGTGAGGACAATGCTTGTCCCGACTGTGCATGCAGACCGTGGCGCCACACTAGTGT

AGGCCTCAGCGGCTGACGCACAAGATCCATTTACCACAGAAACGGAGGTGCTCGAGACCA

GAATGACTTCTCTTGCGGATGTCGTTGGACGATGGAAATCGTGAGGAAGGGGAGGAAACG

AGAGACGCGAAGAGGGCGCAACCCGATTTCGGAGAGGACCGGAAGTGCGTGCCGACCACC

AGGGCGTCTAGCTCAAATGGTAGAGCGCTCGCTTAGCATGCGAGAGGTAATGGGATCGAT

ACCCATGTCGTCCAGTTCTTTTCCGAAATTCCAACTCATTGCGACACTTTTAAACTGCTT

CGATGTTCCGAAAATGTCCGGCCTCTCATCTGATACGTCTTGCTGACACGGGCCGAAATA

GAAAACTCTACTTGCTCTCTGTGGCTGCGTCATATTGCGCCATATCGTGTGTACGGCAAC

GGAGTCAGCCGGATGAACAGCTGCTGTTGACGATTCTGTCCTATTTTCAATTCACGATGA

AAATACTATGAAAAATAGTATGTAGACGTTTTGCTGTAAATGAAGAAATATGCAGAGAAA

CTCGTATCACATGTCGCTGCAAGCCGCCACTAGATCGTCCACAAACCTGTTCCGCTCAGT

TGGCTTTCGTACCATCCGGGCAAGCGCACACAACGGGTCTAGACCGCAGCCTCCTGACTT

CACAAATCTCGAAGTAAGCAGGCTCACTCCTAATTTTCCTCAAGAACCATAAGCATCTAC

CGCCGAGCTGCAACAGTGAGCTCGCCTGTATTCCTCAACCCGCCCTACGACGTCACCCCC

GCGACCGCGGAAATCTGGGTTTTGGATCACGAATCAGTTGACCTGTCCTCCGCTCTAGAG

TCCACTGCATTGAACCGGGAACATTTTTATCGGTTCTACAAGTTTGGAATACGCATGTGT

CTGTTCCCACACCAAGTAACTGCGTCCGTCTGGTCTGTTTCAATTATGTAACTTGGCAAC

GTTGTCGCGATGGCCCTCGCCCGTAATCTGCTCGGATATCACACTGACCATGCCACTTCA

CTTATTTGAGCACATGAAACGGTCGGTGGATGAGCAGTTCAGGACAAACATGCTTGTCGC

TGTCTCAGTCTCTCCAGTTGTTCGGTGTTCTAGGGTGAGTGACGCTGCAGCCAAGCCAGC

CGTAACTCGCGTCTCAAAGGGCACCGGGGCCAGAGTTTGGCAGCCACTTTTTCACACGGC

ACTTCGGCGCACATTTCAGGGTGCACGCGCCAGGCACAGCCGACCGCTTCACGAGGTGTT

AGTGACGCATACCTCGTTCGCGTCTCTCTTCGAATACGGGACACAAACAAATTCCGTTCA

CGACAGATCACCGAAGCGTCATTGGGCACTCATTGTTAACAAACTGCCGTAGCCGATCCG

TTCGCACGTCATACTCGGCCTCGAGCACCGCGCACGTCCTACCCGGAGTCCTCATTGTAT

CCGATCCCGGTACCTCTTTCATTCTACCGCAGCTTCCAGTGTACTTTCCGCGTCACCAAA

GCGGTCAGTAGAACCAGGTTCGAGCTGCTGTTCAGTTTTTTTGACGATCGACGCGTCTTG

CCTTTACTGTTGTGTTCGTACGAATCAGCTGCCGTTTATAGGTAGGCATCATGTGGTTGC

CGGTGTATGTGCCTCTCTTGCTGGTGTTTGGAGTTAGTTTGTCCCTCCCACAAGGAAGCC

TTGGCACAGACTCATCGTCACTCCGGGGAGTGGACGCTGACACTGAAAAGCGGATTAATG

TCGGGAAGAAACACCTGCAGACCCTCCGAAATCTGGAAACTCGGTGCCACGATTCCTTGC

AAGCACTTGTTGTAATCGATGCAGGCAGTAGTTCGACGAGGACGAATGTTTTCTTGGCGA

AAACCCGGTCATGCCCAAATAAAGGCCGTAGCATTGACCCAGACAGTATTCAACTCATCG

GCGCTGGCAAGCGGTTCGCAGGACTTCGTGTAGTTTTAGAAGAGTGGCTGGACACTTACG

CTGGAAAGGACTGGGAGTCG
 * GC% Content for gene (codon optimized):** 51.36%

-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**
 * ( link to DNA Works output text file - **that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences):**

[1] Krug, U., Zebisch, M., Krauss, M., & Strater, N. (2012). Structural Insight into Activation Mechanism of Toxoplasma gondii Nucleoside Triphosphate Diphosphohydrolases by Disulfide Reduction. Journal of Biological Chemistry, 287(5), 3051-3066. Retrieved April 17, 2015, from NCBI.
 * References:**