Target-Serine+Protease+Nonstructural+Protein+3(NS3)

[] [] [] Dengue Virus is a flavivirus carried by mosquitoes and transmitted to monkeys and humans through mosquito bites. Over a third of the wold's population lives in areas at risk for transmission of any of the four strains of Dengue Virus (DENV-1, DENV-2, DENV-3, DENV-4). Dengue transmissions have been high in the recent half century in tropical and subtropical areas; approximately one-hundred-million humans are infected annually. Dengue Virus causes Dengue Fever which may cause high fever, severe headaches and bodily pains, rash, low white cell count, drowsiness, pale skin, breathing difficulties, and mild to severe bleeding. This can be fatal for more extreme cases, especially when the fever becomes hemorrhagic or develops into Dengue shock syndrome. Currently, no vaccines or medications exist for Dengue virus. Analgesics (pain relievers) are suggested, but ibuprofen, Naproxen, and asprin or aspirin containing drugs should be avoided. [] []
 * *Target (protein/gene name): **Serine Protease NS3
 * *NCBI Gene # or RefSeq#: **35662466
 * *Protein ID (NP or XP #) or Wolbachia#: **N/A
 * *Organism (including strain): **//Dengue Virus 3 Singapore/8120/1995//
 * Etiologic Risk Group (see link below): **Risk Group 2 (RG2) - Viruses
 * *Background/Disease Information (sort of like the Intro to your Mini Research Write up): **
 * Essentiality of this protein: **Yes; essential to maturation of virus.
 * Complex of proteins?: **Forms a complex with NS2B to form NS2B(H)-NS3p complex.
 * Druggable Target: **Peptide inhibition was effective against Dengue Virus NS3 protease.

[] [] [] [] [] [] PDB: 3u1i
 * *EC#: **3.4.21.91, 3.6.1.15, 3.6.4.13 (Multiple EC#'s listed)
 * Link to BRENDA EC# page: (In order of EC# respectively) **
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): ** Chromogenic and Fluorescent assay
 * -- link to Sigma (or other company) page for assay or assay reagents (substrates) - ** Currently unavailable
 * -- link (or citation) to paper that contains assay information **
 * -- List cost and quantity of substrate reagents and supplier - N/A, see above **
 * Structure Available (PDB or Homology model) **

[]
 * Current Inhibitors: ** phthalazine-based compound, 7 ; pthalazine-based compound, 2; quanidine 5; quanidine 4; Bz-NKRR-H
 * Expression Information (has it been expressed in bacterial cells): ** In //E. coli//

[]
 * Purification Method: ** Purified from insoluble inclusion bodies by Ni 2+ ion affinity and gel filtration chromatography.
 * Image of protein (PyMol with features delineated and shown separately): **

[] code format="genbank" 1 mnnqrkktgk psinmlkrvr nrvstgsqla krfskgllng qgpmklvmaf iaflrflaip 61 ptagvlarwg tfkksgaikv lkgfkkeisn mlsiinqrkk tslclmmilp aalafhltsr 121 dgeprmivgk nergksllfk tasginmctl iamdlgemcd dtvtykcphi tevepedidc 181 wcnltstwvt ygtcnqageh rrdkrsvala phvgmgldtr tqtwmsaega wrqvekvetw 241 alrhpgftil alflahyigt sltqkvvifi llmlvtpsmt mrcvgvgnrd fveglsgatw 301 vdvvlehggc vttmaknkpt ldielqktea tqlatlrklc iegkitnitt dsrcptqgea 361 vlpeeqdqny vckhtyvdrg wgngcglfgk gslvtcakfq clepiegkvv qyenlkytvi 421 itvhtgdqhq vgnetqgvta eitpqastte ailpeygtlg lecsprtgld fnemilltmk 481 nkawmvhrqw ffdlplpwas gattetptwn rkellvtfkn ahakkqevvv lgsqegamht 541 altgateiqn sggtsifagh lkcrlkmdkl elkgmsyamc tntfvlkkev setqhgtili 601 kveykgedap ckipfstedg qgkahngrli tanpvvtkke epvnieaepp fgesnivigi 661 gdnalkinwy kkgssigkmf eatergarrm ailgdtawdf gsvggvlnsl gkmvhqifgs 721 aytalfsgvs wvmkigigvl ltwiglnskn tsmsfsciai giitlylgav vqadmgcvin 781 wkgkelkcgs gifvtnevht wteqykfqad spkrlataia gawengvcgi rsttrmenll 841 wkqianelny ilwenniklt vvvgdtlgvl eqgkrtltpq pmelkyswkt wgkakivtae 901 tqnssfiidg pntpecpsas rawnvweved ygfgvfttni wlklrevytq lcdhrlmsaa 961 vkderavhad mgywiesqkn gswklekasl ievktctwpk shtlwtngvl esdmiipksl 1021 agpisqhnyr pgyhtqtagp whlgkleldf nycegttvvi tescgtrgps lrtttvsgkl 1081 ihewccrsct lpplrymged gcwygmeirp isekeenmvk slvsagsgkv dnftmgvlcl 1141 ailfeevlrg kfgkkhmiag vfftfvllls gqitwrdmah tlimigsnas drmgmgvtyl 1201 aliatfkiqp flalgfflrk ltsrenlllg vglamattlq lpedieqman gvalglmalk 1261 litqfetyql wtalvsltcs ntiftltvaw rtatlilagv sllpvcqsss mrktdwlpmt 1321 vaamgvpplp lfifslkdtl krrswplneg vmavglvsil assllrndvp magplvaggl 1381 liacyvitgt sadltvekap dvtweeeaeq tgvshnlmit vdddgtmrik ddeteniltv 1441 llktallivs gifpysipat llvwhtwqkq tqrsgvlwdv psppetqkae leegvyrikq 1501 qgifgktqvg vgvqkegvfh tmwhvtrgav lthngkrlep nwasvkkdli sygggwrlsa 1561 qwqkgeevqv iavepgknpk nfqttpgtfq tttgeigaia ldfkpgtsgs piinregkvv 1621 glygngvvtk nggyvsgiaq tnaepdgptp eleeemfkkr nltimdlhpg sgktrkylpa 1681 ivreaikrrl rtlilaptrv vaaemeealk glpiryqtta tksehtgrei vdlmchatft 1741 mrllspvrvp nynliimdea hftdpasiaa rgyistrvgm geaaaifmta tppgtadafp 1801 qsnapiqdee rdiperswns gnewitdfag ktvwfvpsik agndianclr kngkkviqls 1861 rktfdteyqk tklndwdfvv ttdisemgan fkadrvidpr rclkpviltd gpervilagp 1921 mpvtaasaaq rrgrvgrnpq kendqyiftg qplnndedha hwteakmlld nintpegiip 1981 alfepereks aaidgeyrlk gesrktfvel mrrgdlpvwl ahkvasegik ytdrkwcfdg 2041 qrnnqileen mdveiwtkeg ekkklrprwl dartysdpla lkefkdfaag rksialdlvt 2101 eigrvpshla hrtrnaldnl vmlhtsedgg rayrhaveel petmetllll glmilltgga 2161 mlflisgkgi gktsiglicv iassgmlwma evplqwiasa ivleffmmvl lipepekqrt 2221 pqdnqlayvv igiltlaati aanemgllet tkrdlgmske pgvvsptsyl dvdlhpasaw 2281 tlyavattvi tpmlrhtien stanvslaai anqavvlmgl dkgwpiskmd lgvpllalgc 2341 ysqvnpltlt aavlllithy aiigpglqak atreaqkrta agimknptvd gimtidldsv 2401 ifdskfekql gqvmllvlca vqlllmrtsw alcealtlat gpittlwegs pgkfwnttia 2461 vsmanifrgs ylagaglafs imksvgtgkr gtgsqgetlg ekwkkklnql srkefdlykk 2521 sgitevdrte akeglkrget thhavsrgsa klqwfvernm vvpegrvidl gcgrggwsyy 2581 caglkkvtev rgytkggpgh eepvpmstyg wnivklmsgk dvfylppekc dtllcdiges 2641 spsptveesr tirvlkmvep wlknnqfcik vlnpymptvi ehlerlqrkh ggmlvrnpls 2701 rnsthemywi sngtgnivss vnmvsrllln rftmthrrpt iekdvdlgag trhvnaepet 2761 pnmdvigeri krikeehnst whyddenpyk twayhgsyev katgsassmi ngvvklltkp 2821 wdvvpmvtqm amtdttpfgq qrvfkekvdt rtprpmpgtr kameitaewl wrtlgrnkrp 2881 rlctreeftk kvrtnaamga vfteenqwds akaavedeef wklvdrerel hklgkcgscv 2941 ynmmgkrekk lgefgkakgs raiwymwlga rylefealgf lnedhwfsre nsysgvegeg 3001 lhklgyilrd iskipggamy addtagwdtr iteddlhnee kiiqqmdpeh rqlanaifkl 3061 tyqnkvvkvq rptptgtvmd iisrkdqrgs gqlgtyglnt ftnmeaqlvr qmegegvltk 3121 adlenphlle kkitqwletk gverlkrmai sgddcvvkpi ddrfanalla lndmgkvrkd 3181 ipqwqpskgw hdwqqvpfcs hhfhelimkd grklvvpcrp qdeligrari sqgagwslre 3241 taclgkayaq mwslmyfhrr dlrlasnaic savpvhwvpt srttwsihah hqwmttedml 3301 tvwnrvwiee npwmedktpv ttwenvpylg kredqwcgsl igltsratwa qniptaiqqv 3361 rsligneefl dympsmkrfr keeesegaiw code [] code ATGAACAACCAACGGAAGAAGACGGGAAAACCGTCTATCAATATGCTGAAACGCGTGAGAAACCGTGTGT CAACTGGATCACAGTTGGCGAAGAGATTCTCAAAAGGACTGCTGAACGGCCAGGGACCAATGAAATTGGT TATGGCGTTCATAGCTTTCCTCAGATTTCTAGCCATTCCACCAACAGCAGGAGTCTTGGCTAGATGGGGA ACCTTCAAGAAGTCGGGGGCCATTAAGGTCCTGAAAGGCTTCAAGAAGGAGATCTCAAACATGCTGAGCA TAATCAACCAACGGAAAAAGACATCGCTCTGTCTCATGATGATATTGCCAGCAGCACTTGCTTTCCACTT GACTTCACGAGATGGAGAGCCGCGCATGATTGTGGGGAAGAATGAAAGAGGTAAATCCCTACTTTTTAAG ACAGCCTCTGGAATCAACATGTGCACACTCATAGCCATGGATTTGGGAGAGATGTGTGATGACACGGTCA CTTACAAATGCCCCCACATTACCGAAGTGGAACCTGAAGACATTGACTGCTGGTGCAACCTTACATCAAC ATGGGTGACTTATGGAACGTGCAATCAAGCTGGAGAGCATAGACGCGACAAGAGATCAGTGGCGTTAGCT CCCCATGTCGGCATGGGACTGGACACACGCACCCAAACCTGGATGTCGGCTGAAGGAGCTTGGAGACAAG TCGAGAAGGTAGAGACATGGGCCCTTAGGCACCCAGGGTTCACCATACTAGCCCTATTTCTCGCCCATTA CATAGGCACTTCCCTGACCCAGAAGGTGGTTATTTTCATATTATTAATGCTGGTCACCCCATCCATGACA ATGAGATGTGTGGGAGTAGGAAACAGAGATTTTGTGGAAGGGCTATCAGGAGCTACGTGGGTTGACGTGG TGCTCGAGCACGGGGGGTGTGTGACTACCATGGCTAAGAACAAGCCCACGCTGGATATAGAGCTTCAGAA GACCGAGGCCACCCAACTGGCGACCCTAAGGAAGCTATGCATTGAGGGGAAAATTACCAACATAACAACT GACTCAAGATGTCCTACCCAAGGGGAAGCGGTTTTGCCTGAGGAGCAGGACCAGAACTACGTGTGTAAGC ATACATACGTAGACAGAGGTTGGGGGAACGGTTGTGGTTTGTTTGGCAAAGGAAGCTTGGTAACATGTGC GAAATTTCAATGCCTGGAACCAATAGAGGGAAAAGTGGTGCAATATGAGAACCTCAAATACACCGTCATC ATTACAGTGCACACAGGAGACCAACACCAGGTGGGAAATGAAACGCAAGGAGTCACGGCTGAGATAACAC CTCAGGCATCAACCACTGAAGCCATCTTGCCTGAATATGGAACCCTTGGGCTAGAATGCTCACCACGGAC AGGTTTGGATTTCAATGAAATGATCTTACTAACAATGAAGAACAAAGCATGGATGGTACATAGACAATGG TTCTTTGACCTACCTCTACCATGGGCATCAGGAGCTACAACAGAAACACCAACCTGGAACAGGAAGGAGC TTCTTGTGACATTCAAAAACGCACATGCGAAAAAACAAGAAGTAGTTGTCCTTGGATCGCAAGAGGGAGC AATGCATACCGCACTGACAGGAGCTACAGAAATCCAAAACTCAGGAGGCACAAGCATTTTCGCGGGGCAC TTAAAATGTAGACTTAAGATGGACAAATTGGAACTCAAGGGGATGAGCTATGCAATGTGCACGAATACCT TTGTGTTGAAGAAAGAAGTCTCAGAAACGCAGCACGGGACAATACTCATTAAGGTTGAGTACAAAGGGGA AGATGCACCTTGCAAGATTCCCTTTTCCACAGAGGATGGACAAGGGAAAGCTCATAATGGCAGACTGATC ACAGCCAACCCTGTGGTGACTAAGAAGGAGGAGCCTGTCAATATTGAGGCTGAACCTCCTTTTGGGGAAA GCAATATAGTAATTGGAATTGGAGACAACGCCTTGAAAATCAACTGGTACAAGAAGGGGAGCTCGATTGG GAAGATGTTCGAGGCCACTGAAAGGGGTGCAAGGCGCATGGCCATCTTGGGAGACACAGCTTGGGACTTT GGATCAGTGGGTGGTGTTCTGAACTCATTAGGCAAAATGGTGCACCAAATATTTGGAAGTGCTTATACAG CCCTGTTCAGTGGAGTCTCTTGGGTGATGAAAATTGGAATAGGTGTCCTCTTGACTTGGATAGGGTTGAA TTCAAAAAACACATCCATGTCATTTTCATGCATTGCGATAGGAATCATTACACTCTATCTGGGAGCTGTG GTACAAGCTGACATGGGGTGTGTCATAAACTGGAAGGGCAAAGAACTCAAATGTGGAAGCGGAATTTTCG TCACCAATGAGGTCCATACCTGGACAGAGCAATACAAATTCCAAGCAGACTCCCCAAAAAGATTGGCAAC AGCCATTGCAGGCGCCTGGGAGAATGGAGTGTGTGGAATTAGGTCAACAACCAGAATGGAGAATCTCTTG TGGAAGCAAATAGCCAATGAACTGAACTACATATTATGGGAAAACAATATCAAATTAACGGTAGTTGTGG GCGATACACTTGGGGTCTTAGAGCAAGGGAAAAGAACACTAACACCACAACCCATGGAGCTAAAATACTC ATGGAAAACGTGGGGAAAGGCAAAAATAGTGACAGCTGAAACACAAAATTCCTCTTTCATAATAGACGGG CCAAACACACCGGAGTGTCCAAGTGCCTCAAGAGCATGGAATGTGTGGGAGGTGGAAGATTACGGGTTCG GAGTCTTCACAACCAACATATGGCTGAAACTCCGAGAGGTCTACACCCAACTATGTGACCATAGGCTAAT GTCGGCAGCTGTCAAGGATGAGAGGGCCGTGCATGCCGACATGGGCTACTGGATAGAAAGCCAAAAGAAT GGAAGTTGGAAGCTAGAAAAAGCATCCCTCATAGAGGTAAAAACCTGCACATGGCCAAAATCACACACTC TCTGGACTAATGGTGTGCTAGAGAGTGACATGATCATCCCAAAGAGTCTAGCTGGTCCTATCTCACAACA CAACTACAGGCCCGGGTACCACACCCAAACGGCAGGACCCTGGCACTTAGGAAAATTGGAGCTGGACTTC AACTACTGTGAAGGAACAACAGTTGTCATCACAGAAAGCTGTGGGACAAGAGGCCCATCATTGAGAACAA CAACAGTGTCAGGGAAGTTGATACACGAATGGTGTTGCCGCTCGTGCACACTTCCCCCCCTGCGATACAT GGGAGAAGACGGCTGCTGGTATGGCATGGAAATCAGACCCATCAGTGAGAAAGAAGAGAACATGGTAAAG TCTTTAGTCTCAGCGGGAAGTGGAAAGGTGGACAACTTCACAATGGGTGTCTTGTGTTTGGCAATCCTCT TTGAAGAGGTGTTGAGAGGAAAATTTGGGAAGAAACACATGATTGCAGGGGTTTTCTTTACGTTTGTGCT CCTTCTCTCAGGGCAAATAACATGGAGAGACATGGCGCACACACTAATAATGATCGGGTCCAACGCCTCT GACAGGATGGGAATGGGCGTCACCTACCTAGCTCTAATTGCAACATTTAAAATCCAGCCATTCTTGGCTT TGGGATTTTTCCTAAGAAAGCTGACATCTAGAGAAAATTTATTGTTAGGAGTTGGGTTGGCCATGGCAAC AACGTTACAACTGCCAGAGGACATTGAACAAATGGCAAATGGAGTCGCTCTGGGGCTCATGGCTCTTAAA CTGATAACACAATTTGAAACATACCAATTGTGGACGGCATTAGTCTCCTTAACGTGTTCAAACACAATTT TTACGTTGACTGTTGCCTGGAGAACAGCCACTCTGATTTTGGCCGGAGTTTCGCTTTTACCAGTGTGCCA GTCTTCAAGCATGAGGAAAACAGATTGGCTCCCAATGACAGTGGCAGCTATGGGAGTTCCACCCCTTCCA CTTTTTATTTTTAGCTTGAAAGACACACTCAAAAGGAGAAGCTGGCCACTGAATGAAGGGGTGATGGCTG TTGGGCTTGTGAGCATTCTGGCCAGTTCTCTCCTTAGAAATGATGTGCCCATGGCTGGACCATTAGTGGC CGGGGGCTTGCTGATAGCGTGCTACGTCATAACTGGCACGTCAGCGGACCTCACTGTAGAAAAAGCCCCA GATGTAACATGGGAGGAAGAGGCTGAGCAGACAGGAGTGTCCCACAACTTAATGATCACAGTTGATGATG ATGGAACAATGAGAATAAAAGATGATGAGACTGAGAACATCCTAACAGTGCTTTTAAAAACAGCATTACT AATAGTATCAGGCATTTTTCCATACTCCATACCCGCAACATTGTTGGTCTGGCACACTTGGCAAAAACAA ACCCAAAGATCCGGCGTTTTATGGGACGTACCCAGCCCCCCAGAGACACAGAAAGCAGAACTGGAAGAAG GGGTTTATAGGATCAAACAGCAAGGAATTTTTGGGAAAACCCAAGTAGGGGTTGGAGTACAGAAAGAAGG AGTCTTCCACACCATGTGGCACGTCACAAGAGGGGCAGTGTTGACACATAATGGGAAAAGACTGGAACCA AACTGGGCTAGTGTGAAAAAAGATCTGATTTCATATGGAGGAGGATGGAGACTGAGCGCACAATGGCAAA AGGGGGAGGAGGTGCAGGTTATTGCCGTAGAGCCAGGGAAGAACCCAAAGAACTTTCAAACCACGCCAGG CACTTTCCAGACTACTACAGGGGAAATAGGAGCAATTGCACTGGATTTCAAGCCTGGAACTTCAGGATCT CCTATCATAAATAGAGAGGGAAAGGTAGTGGGACTGTATGGCAATGGAGTGGTTACAAAGAATGGTGGCT ATGTCAGCGGAATAGCGCAAACAAATGCAGAACCAGATGGACCGACACCAGAGTTGGAAGAAGAGATGTT CAAAAAGCGAAACCTGACCATAATGGATCTTCATCCTGGGTCAGGAAAGACACGGAAATACCTTCCAGCT ATTGTCAGAGAGGCAATCAAGAGACGTTTAAGAACCTTAATTTTGGCACCGACAAGGGTGGTTGCAGCTG AGATGGAAGAAGCATTGAAAGGGCTCCCAATAAGGTACCAAACAACAGCAACAAAATCTGAACACACAGG AAGAGAGATTGTTGATCTAATGTGCCACGCAACGTTCACAATGCGTTTGCTGTCACCAGTTAGGGTTCCA AATTACAACTTGATAATAATGGATGAGGCCCATTTCACAGACCCAGCCAGTATAGCGGCTAGAGGGTACA TATCAACTCGTGTTGGAATGGGAGAGGCAGCCGCAATCTTCATGACAGCAACACCCCCTGGAACAGCTGA TGCCTTTCCTCAGAGCAACGCTCCAATTCAAGATGAAGAAAGGGACATACCAGAACGCTCATGGAATTCA GGCAATGAATGGATTACCGACTTCGCTGGGAAAACGGTGTGGTTTGTCCCTAGCATTAAAGCCGGAAATG ACATAGCAAACTGCTTGCGAAAAAACGGGAAAAAAGTCATTCAACTTAGTAGGAAGACTTTTGACACAGA ATATCAGAAGACTAAACTGAATGATTGGGACTTTGTGGTGACAACTGACATTTCAGAAATGGGGGCCAAT TTCAAAGCAGATAGAGTGATCGACCCAAGAAGATGTCTCAAACCAGTGATCTTGACAGATGGACCAGAGC GGGTGATCCTGGCCGGACCAATGCCAGTCACCGCGGCGAGTGCTGCGCAAAGGAGAGGGAGAGTTGGCAG GAACCCACAAAAAGAGAATGACCAGTACATATTCACGGGCCAGCCTCTCAACAATGATGAAGACCATGCT CACTGGACAGAAGCAAAAATGCTGCTGGACAACATCAACACACCAGAAGGGATTATACCAGCTCTCTTTG AACCAGAAAGGGAGAAGTCAGCCGCCATAGACGGTGAGTATCGCCTGAAGGGTGAGTCCAGGAAGACTTT CGTGGAACTCATGAGGAGGGGTGACCTTCCAGTTTGGTTAGCCCATAAAGTAGCATCAGAAGGAATCAAA TACACAGATAGAAAATGGTGCTTTGATGGGCAACGCAATAATCAAATTTTAGAGGAGAACATGGATGTGG AAATTTGGACAAAGGAAGGAGAAAAGAAAAAATTGAGACCTAGGTGGCTTGATGCCCGCACTTATTCAGA TCCATTGGCACTCAAGGAATTCAAGGACTTTGCGGCTGGCAGAAAGTCAATCGCCCTTGATCTTGTGACA GAAATAGGAAGAGTGCCTTCACATCTAGCCCACAGAACAAGAAACGCTCTGGACAATCTGGTGATGCTGC ATACGTCAGAAGATGGCGGTAGGGCTTACAGGCATGCGGTGGAGGAACTACCAGAAACAATGGAAACACT CCTACTCTTGGGACTAATGATCTTGTTGACAGGTGGAGCAATGCTTTTCTTGATATCAGGTAAAGGGATT GGAAAGACTTCAATAGGACTCATTTGTGTAATCGCTTCCAGCGGCATGTTGTGGATGGCCGAAGTTCCAC TCCAATGGATCGCGTCGGCTATAGTCCTGGAGTTTTTTATGATGGTGTTGCTCATACCAGAACCAGAAAA GCAGAGAACCCCCCAAGACAACCAACTCGCATATGTCGTGATAGGCATACTTACATTGGCTGCAACAATA GCAGCCAATGAAATGGGACTGCTGGAAACCACAAAGAGAGACTTAGGAATGTCTAAGGAGCCAGGTGTTG TTTCTCCAACCAGCTATTTGGATGTGGACTTGCACCCAGCATCAGCCTGGACATTGTACGCCGTGGCCAC TACAGTAATAACACCAATGTTAAGACATACCATAGAGAATTCTACAGCAAATGTGTCCCTGGCAGCTATA GCCAACCAGGCAGTGGTCCTGATGGGTTTGGACAAAGGATGGCCAATATCAAAAATGGACTTAGGCGTGC CACTACTGGCACTGGGTTGCTATTCACAAGTGAACCCACTGACTCTAACTGCGGCAGTACTTTTGCTAAT CACACATTATGCTATCATAGGTCCAGGATTGCAAGCAAAAGCCACCCGTGAAGCTCAGAAAAGGACAGCT GCTGGAATAATGAAGAATCCAACAGTGGATGGGATAATGACAATAGACCTAGATTCTGTAATATTTGATT CAAAATTTGAAAAACAACTGGGACAGGTTATGCTCCTGGTTTTGTGCGCAGTCCAACTCTTGCTAATGAG AACATCATGGGCCTTGTGTGAAGCTTTAACTCTAGCTACAGGACCAATAACAACACTCTGGGAAGGATCA CCTGGTAAGTTCTGGAACACCACGATAGCTGTTTCCATGGCGAACATTTTTAGAGGGAGCTATTTAGCAG GAGCTGGGCTTGCTTTTTCTATTATGAAATCAGTTGGAACAGGAAAAAGAGGAACAGGCTCACAAGGTGA AACTTTAGGAGAAAAATGGAAAAAGAAATTAAATCAATTATCCCGGAAAGAGTTTGACCTTTACAAGAAA TCTGGAATCACTGAAGTGGATAGAACAGAAGCCAAAGAAGGGTTGAAAAGAGGAGAGACAACACATCATG CCGTGTCCCGAGGTAGCGCAAAACTTCAATGGTTTGTGGAAAGAAACATGGTCGTTCCCGAAGGAAGAGT CATAGACTTGGGCTGTGGAAGAGGAGGCTGGTCATATTACTGTGCAGGACTGAAAAAAGTCACAGAAGTG CGAGGATACACAAAAGGCGGTCCAGGACACGAAGAACCAGTACCTATGTCTACATATGGATGGAACATAG TTAAGTTAATGAGCGGAAAGGATGTGTTCTATCTCCCACCTGAAAAGTGTGATACCCTGTTGTGTGACAT TGGAGAATCTTCACCAAGCCCAACAGTGGAAGAGAGCAGAACTATAAGAGTTTTGAAGATGGTTGAACCA TGGCTAAAAAACAACCAGTTTTGCATTAAAGTTTTGAACCCTTACATGCCAACTGTGATTGAGCACCTAG AAAGACTACAAAGGAAACATGGAGGAATGCTTGTGAGAAATCCACTTTCACGAAACTCCACGCACGAAAT GTACTGGATATCTAATGGCACAGGTAACATTGTCTCTTCAGTCAACATGGTGTCTAGATTGCTACTGAAC AGGTTCACGATGACACACAGGAGACCCACCATAGAGAAAGATGTGGATTTAGGAGCAGGAACTCGACATG TTAATGCGGAACCAGAAACACCCAACATGGATGTCATTGGGGAAAGAATAAAAAGGATCAAGGAGGAGCA TAATTCAACATGGCACTATGATGACGAAAACCCCTACAAAACGTGGGCTTACCATGGATCCTATGAAGTC AAAGCCACAGGCTCAGCCTCCTCCATGATAAATGGAGTCGTGAAACTCCTCACCAAACCATGGGATGTGG TGCCCATGGTGACACAGATGGCAATGACAGACACAACTCCATTTGGCCAGCAGAGAGTCTTTAAAGAGAA AGTGGACACCAGGACGCCCAGGCCCATGCCAGGGACAAGAAAGGCTATGGAGATCACAGCGGAGTGGCTC TGGAGAACCCTGGGAAGGAACAAAAGACCCAGATTATGCACAAGGGAAGAGTTTACAAAAAAGGTCAGAA CTAACGCAGCCATGGGCGCCGTTTTCACAGAGGAGAACCAATGGGACAGTGCGAAAGCTGCTGTTGAGGA TGAAGAATTTTGGAAACTTGTGGACAGAGAACGTGAACTCCACAAATTGGGCAAATGTGGAAGCTGCGTT TATAACATGATGGGCAAGAGAGAGAAAAAACTTGGAGAGTTTGGCAAAGCAAAAGGCAGTAGAGCTATAT GGTACATGTGGTTGGGAGCCAGGTACCTTGAGTTCGAAGCCCTTGGATTCTTAAATGAAGACCACTGGTT CTCGCGTGAAAACTCTTACAGTGGAGTAGAAGGAGAAGGACTGCACAAGCTAGGCTACATATTAAGGGAC ATTTCCAAGATACCCGGAGGAGCCATGTATGCTGATGACACAGCTGGTTGGGACACAAGAATAACAGAAG ATGACCTGCACAATGAGGAAAAGATCATACAGCAAATGGACCCTGAACACAGGCAGTTAGCGAACGCTAT ATTCAAGCTCACATACCAAAACAAAGTGGTCAAAGTTCAACGACCGACTCCAACGGGCACGGTAATGGAT ATTATATCTAGGAAAGACCAAAGGGGCAGTGGACAACTGGGAACTTATGGCCTGAATACATTCACCAACA TGGAAGCCCAGTTAGTCAGACAAATGGAAGGAGAAGGTGTGCTGACAAAGGCAGACCTCGAGAACCCTCA TCTGCTAGAGAAGAAAATCACACAATGGTTGGAAACCAAAGGAGTGGAGAGGTTAAAAAGAATGGCCATT AGCGGGGATGATTGCGTGGTGAAACCAATCGATGACAGGTTCGCTAATGCCCTGCTTGCTTTGAACGATA TGGGAAAGGTTCGGAAAGACATACCTCAATGGCAGCCATCAAAGGGATGGCATGATTGGCAACAGGTTCC TTTCTGCTCCCACCACTTTCATGAATTGATCATGAAAGATGGAAGAAAGTTGGTGGTTCCCTGCAGACCC CAGGACGAACTAATAGGAAGAGCAAGAATCTCTCAAGGAGCGGGATGGAGCCTTAGAGAAACTGCATGTC TGGGGAAAGCCTACGCCCAAATGTGGAGTCTCATGTATTTTCACAGAAGAGATCTCAGATTAGCATCCAA CGCCATATGTTCAGCAGTACCAGTCCACTGGGTTCCCACAAGTAGAACGACATGGTCTATTCATGCTCAC CATCAGTGGATGACTACAGAAGACATGCTTACTGTTTGGAACAGGGTGTGGATAGAGGAAAATCCATGGA TGGAAGACAAAACTCCAGTTACAACTTGGGAAAATGTTCCATATCTAGGAAAGAGAGAAGACCAATGGTG TGGATCACTTATTGGTCTCACTTCCAGAGCAACCTGGGCCCAGAACATACCCACAGCAATTCAACAGGTG AGAAGCCTTATAGGCAATGAAGAGTTCCTGGACTACATGCCTTCAATGAAGAGATTCAGGAAGGAAGAGG AGTCGGAGGGAGCCATTTGGTAA code
 * Figure 1:** Serine protease nonstructural protein 3 (shown as lines with green carbons, red oxygens, blue nitrogens) found in Dengue Virus covalently bound to a peptide (PDB Identifier: 3u1i). A sulfate ion (red oxygen, yellow sulfur sticks) is the ligand in the active site.
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **
 * *length of your protein in Amino Acids: ** 3391 Amino Acids
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website: ** 818568.5 kDa
 * Molar Extinction coefficient of your protein at 280 nm wavelength: ** 130500 1/(Mcm)
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html): N/A
 * *CDS Gene Sequence (paste as text only): **

ATG AAC AAT CAA AGA AAA AAG ACA GGA AAG
 * *GC% Content for gene: ** 46.7%
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **

CCC TCA ATC AAT ATG CTC AAA AGA GTC AGG

AAC AGG GTC AGC ACA GGA AGT CAA CTC GCT

AAA AGA TTC TCT AAA GGA CTG TTG AAC GGA

CAG GGA CCA ATG AAG TTG GTG ATG GCA TTC

ATC GCC TTT CTC AGA TTC CTC GCA ATA CCA

CCT ACA GCA GGG GTC TTA GCA AGA TGG GGA

ACA TTT AAA AAG AGT GGA GCT ATA AAG GTC

CTA AAA GGG TTT AAG AAA GAA ATA TCA AAT

ATG CTG TCC ATA ATT AAT CAG AGA AAA AAG

ACT AGT CTG TGT CTC ATG ATG ATA CTG CCA

GCT GCA CTG GCT TTC CAC CTC ACC TCA AGA

GAC GGA GAG CCA AGA ATG ATC GTG GGA AAA

AAC GAG AGA GGA AAG TCC CTT CTA TTT AAG

ACC GCC TCA GGA ATA AAC ATG TGT ACA CTC

ATC GCA ATG GAT CTT GGA GAA ATG TGC GAT

GAC ACA GTT ACA TAT AAG TGT CCA CAT ATC

ACA GAG GTT GAA CCA GAG GAT ATC GAC TGT

TGG TGT AAT CTC ACT TCA ACT TGG GTG ACT

TAC GGA ACA TGC AAC CAG GCA GGG GAG CAC

AGA AGG GAC AAA AGG AGC GTG GCT CTG GCA

CCA CAC GTG GGC ATG GGA CTC GAT ACC AGG

ACA CAG ACA TGG ATG TCT GCA GAG GGA GCT

TGG AGA CAA GTT GAA AAG GTT GAA ACA TGG

GCC TTG AGA CAT CCA GGA TTT ACC ATT TTG

GCC CTT TTT CTC GCA CAT TAT ATA GGA ACA

TCA CTT ACT CAA AAG GTG GTT ATC TTT ATA

CTC TTA ATG TTG GTG ACA CCA TCA ATG ACA

ATG AGG TGC GTT GGC GTG GGA AAC AGA GAC

TTC GTG GAG GGA CTT AGC GGA GCA ACC TGG

GTG GAT GTT GTC TTG GAA CAT GGC GGA TGC

GTC ACA ACT ATG GCA AAA AAC AAA CCA ACC

CTG GAT ATA GAA CTG CAA AAA ACT GAA GCA

ACT CAG TTG GCT ACA CTA AGA AAA CTA TGC

ATA GAA GGC AAA ATC ACT AAT ATT ACA ACC

GAC TCC AGG TGT CCA ACC CAA GGA GAG GCC

GTG TTA CCA GAG GAA CAG GAT CAA AAT TAT

GTG TGT AAA CAC ACT TAT GTG GAT AGA GGC

TGG GGA AAT GGA TGC GGG CTC TTC GGG AAG

GGC TCT CTC GTC ACA TGC GCA AAG TTC CAA

TGT TTG GAA CCA ATA GAA GGA AAG GTC GTG

CAA TAC GAG AAC CTT AAA TAT ACA GTT ATA

ATT ACA GTG CAC ACA GGA GAT CAA CAC CAG

GTG GGC AAT GAG ACA CAA GGA GTG ACA GCC

GAG ATA ACA CCC CAG GCT TCA ACA ACC GAA

GCA ATA TTG CCC GAA TAC GGA ACT CTG GGA

TTG GAG TGC AGC CCC AGA ACT GGA TTG GAT

TTT AAT GAA ATG ATA TTG CTG ACA ATG AAA

AAC AAG GCC TGG ATG GTC CAC AGA CAA TGG

TTT TTC GAC CTC CCA CTG CCA TGG GCA AGC

GGG GCT ACA ACT GAG ACC CCA ACT TGG AAT

AGG AAA GAA CTC CTT GTT ACA TTC AAA AAT

GCC CAC GCA AAA AAG CAA GAA GTT GTC GTG

CTT GGA AGC CAG GAA GGA GCT ATG CAC ACA

GCT CTC ACA GGG GCT ACA GAG ATA CAA AAC

TCA GGA GGC ACT TCC ATT TTT GCA GGC CAC

TTG AAG TGC AGA TTG AAA ATG GAT AAA CTG

GAA TTA AAG GGA ATG TCA TAC GCC ATG TGT

ACA AAT ACA TTC GTG CTG AAG AAA GAG GTT

AGC GAA ACA CAG CAT GGA ACC ATC TTA ATT

AAG GTG GAA TAT AAA GGA GAA GAC GCT CCT

TGT AAA ATT CCA TTT TCC ACT GAA GAC GGA

CAA GGA AAA GCA CAT AAC GGC AGA CTC ATC

ACC GCA AAT CCC GTG GTT ACA AAA AAG GAA

GAG CCT GTG AAC ATC GAA GCC GAG CCC CCA

TTC GGG GAG AGC AAT ATC GTG ATC GGA ATA

GGA GAC AAT GCA CTA AAA ATT AAT TGG TAT

AAA AAG GGA TCA AGC ATC GGA AAG ATG TTC

GAG GCT ACT GAA AGA GGA GCC AGA AGG ATG

GCC ATT TTG GGA GAT ACA GCA TGG GAC TTC

GGG TCC GTG GGA GGC GTC TTG AAT TCA CTG

GGA AAA ATG GTG CAC CAA ATA TTT GGA TCA

GCA TAT ACT GCA TTG TTC TCA GGA GTC TCA

TGG GTG ATG AAG ATA GGA ATA GGA GTC CTC

TTG ACA TGG ATT GGA CTG AAC TCA AAA AAT

ACA AGT ATG AGT TTC TCC TGT ATA GCT ATT

GGA ATA ATC ACC TTG TAC CTG GGA GCT GTT

GTG CAG GCA GAC ATG GGA TGC GTG ATT AAC

TGG AAA GGC AAA GAA CTG AAG TGC GGA TCT

GGC ATC TTT GTC ACA AAC GAG GTC CAT ACA

TGG ACA GAA CAG TAC AAG TTT CAA GCA GAT

TCA CCA AAA AGA CTC GCT ACT GCT ATT GCT

GGA GCA TGG GAG AAC GGA GTC TGT GGA ATA

AGA AGC ACC ACT AGA ATG GAA AAT TTG TTA

TGG AAG CAA ATA GCA AAT GAA CTG AAT TAT

ATA TTG TGG GAA AAT AAC ATA AAG TTA ACA

GTT GTG GTT GGA GAC ACA CTA GGA GTC CTA

GAA CAA GGA AAA AGG ACA CTG ACA CCT CAA

CCA ATG GAG TTA AAA TAC TCT TGG AAA ACA

TGG GGC AAG GCT AAG ATA GTG ACC GCA GAG

ACA CAG AAT TCA AGC TTC ATT ATA GAC GGC

CCA AAC ACT CCA GAA TGT CCA TCA GCC TCA

AGA GCT TGG AAT GTC TGG GAA GTC GAA GAT

TAC GGG TTC GGG GTC TTT ACC ACA AAT ATC

TGG CTG AAG CTG AGA GAA GTG TAC ACA CAG

TTG TGC GAC CAT AGA TTA ATG TCC GCA GCT

GTT AAG GAC GAG AGA GCA GTT CAC GCC GAC

ATG GGA TAT TGG ATT GAA TCA CAA AAA AAC

GGC TCT TGG AAA CTA GAG AAA GCA TCA CTC

ATC GAG GTG AAA ACT TGC ACA TGG CCA AAA

TCA CAC ACA TTG TGG ACA AAT GGA GTG TTA

GAG TCA GAT ATG ATA ATT CCA AAG TCC CTG

GCT GGG CCA ATT AGT CAA CAT AAC TAT AGA

CCA GGA TAT CAC ACA CAG ACA GCT GGG CCA

TGG CAC TTG GGA AAA CTT GAG TTG GAC TTT

AAT TAT TGT GAA GGG ACC ACT GTG GTT ATT

ACA GAG AGC TGC GGA ACA AGA GGA CCC TCC

CTA AGA ACC ACA ACT GTT TCT GGG AAA TTA

ATA CAC GAA TGG TGT TGC AGA TCA TGT ACA

TTG CCC CCA TTG AGG TAT ATG GGG GAG GAT

GGA TGT TGG TAT GGA ATG GAG ATA AGA CCA

ATA TCT GAA AAG GAG GAA AAC ATG GTT AAA

TCC CTT GTG AGC GCT GGA TCA GGG AAA GTT

GAC AAC TTC ACA ATG GGG GTG TTA TGC TTG

GCT ATT CTT TTT GAG GAA GTG CTA AGA GGA

AAG TTC GGA AAA AAG CAT ATG ATT GCC GGA

GTG TTC TTT ACA TTT GTC CTA TTA CTA TCA

GGG CAA ATA ACA TGG AGA GAC ATG GCA CAT

ACA CTA ATA ATG ATT GGA TCA AAC GCC TCA

GAC AGA ATG GGG ATG GGG GTT ACA TAC CTG

GCA CTG ATT GCA ACA TTT AAA ATT CAA CCA

TTC TTG GCC TTA GGG TTC TTT CTC AGA AAA

TTG ACA TCA AGG GAA AAC CTA TTA CTG GGA

GTC GGC CTG GCA ATG GCT ACA ACC CTA CAA

CTC CCA GAA GAC ATC GAA CAG ATG GCC AAC

GGG GTG GCA CTT GGC CTG ATG GCT CTT AAA

CTT ATA ACA CAA TTC GAA ACA TAT CAG CTT

TGG ACA GCT CTG GTT TCA CTT ACA TGT AGT

AAT ACA ATC TTC ACA CTT ACA GTG GCT TGG

AGA ACA GCC ACA TTG ATT CTA GCA GGA GTT

AGC CTT TTG CCC GTC TGC CAA TCA TCT AGC

ATG AGA AAG ACT GAT TGG TTG CCT ATG ACA

GTG GCT GCC ATG GGA GTG CCT CCA CTG CCT

CTT TTT ATT TTC TCA TTA AAA GAC ACA TTG

AAA AGA AGG TCA TGG CCA TTG AAC GAG GGG

GTT ATG GCA GTC GGA CTC GTG AGT ATC CTA

GCA TCA TCT TTA CTT AGA AAT GAC GTC CCA

ATG GCA GGA CCA TTG GTG GCC GGG GGA CTG

CTT ATT GCC TGC TAC GTC ATA ACA GGA ACT

TCA GCT GAT CTG ACT GTT GAA AAG GCA CCA

GAT GTG ACT TGG GAA GAG GAA GCC GAG CAA

ACA GGA GTC AGC CAT AAC CTA ATG ATA ACT

GTT GAC GAT GAC GGC ACA ATG AGA ATA AAG

GAT GAC GAA ACA GAA AAC ATA CTG ACA GTG

TTG CTG AAA ACA GCA TTG TTA ATC GTT TCT

GGC ATA TTC CCC TAT AGC ATT CCC GCC ACC

TTG TTA GTT TGG CAC ACA TGG CAA AAA CAA

ACC CAA AGA TCT GGA GTG CTG TGG GAC GTG

CCT TCA CCT CCC GAA ACA CAG AAA GCT GAG

CTA GAA GAG GGA GTC TAC AGA ATC AAA CAA

CAG GGG ATA TTT GGG AAA ACA CAA GTC GGC

GTG GGA GTG CAA AAG GAA GGC GTG TTT CAC

ACA ATG TGG CAC GTC ACA AGA GGG GCT GTC

TTG ACA CAT AAC GGA AAG AGA CTG GAG CCT

AAT TGG GCT AGT GTC AAG AAA GAT CTC ATC

AGC TAT GGA GGC GGG TGG AGG TTG TCA GCA

CAG TGG CAG AAG GGA GAG GAA GTG CAA GTG

ATA GCC GTG GAA CCC GGC AAA AAT CCA AAA

AAC TTT CAA ACC ACA CCC GGG ACC TTC CAA

ACA ACT ACA GGA GAA ATT GGG GCA ATA GCC

TTA GAC TTC AAA CCA GGG ACT TCT GGA TCA

CCC ATT ATC AAT AGG GAG GGA AAG GTG GTT

GGA CTG TAC GGC AAC GGA GTG GTT ACA AAG

AAC GGA GGG TAC GTG AGT GGA ATT GCA CAG

ACA AAT GCT GAA CCC GAT GGA CCA ACA CCA

GAA TTG GAG GAA GAG ATG TTT AAA AAG AGA

AAT TTG ACA ATA ATG GAT CTT CAC CCC GGA

TCA GGA AAG ACA AGG AAA TAC CTA CCA GCT

ATA GTG AGA GAA GCT ATA AAA AGG AGA TTG

AGG ACC TTG ATA CTA GCA CCA ACA AGA GTG

GTC GCT GCA GAG ATG GAG GAA GCA CTC AAA

GGA CTC CCT ATC AGG TAC CAG ACA ACT GCC

ACC AAA AGC GAA CAT ACT GGG AGA GAA ATC

GTC GAC TTG ATG TGC CAT GCC ACA TTC ACA

ATG AGA CTC CTA AGC CCC GTG AGG GTG CCA

AAC TAC AAC TTG ATT ATC ATG GAT GAG GCA

CAC TTT ACT GAC CCT GCC TCT ATT GCA GCT

AGA GGG TAT ATA AGC ACA AGA GTG GGG ATG

GGG GAA GCA GCC GCA ATA TTT ATG ACA GCC

ACA CCA CCT GGA ACT GCT GAT GCA TTC CCT

CAG TCA AAC GCC CCA ATA CAG GAT GAG GAA

AGA GAT ATC CCA GAA AGA TCA TGG AAC TCA

GGA AAT GAA TGG ATT ACA GAC TTC GCT GGC

AAG ACA GTG TGG TTT GTG CCA TCA ATT AAG

GCT GGC AAT GAC ATC GCC AAC TGT CTT AGA

AAG AAC GGA AAA AAG GTG ATA CAG CTA TCA

AGG AAA ACC TTC GAT ACA GAA TAC CAA AAG

ACA AAG CTG AAC GAT TGG GAC TTT GTT GTG

ACA ACC GAC ATA TCT GAA ATG GGA GCC AAC

TTC AAG GCA GAC AGA GTG ATA GAT CCA AGG

AGA TGC TTG AAA CCT GTG ATA TTA ACA GAC

GGA CCT GAG AGA GTT ATC CTA GCT GGC CCA

ATG CCC GTG ACC GCC GCA TCA GCC GCT CAG

AGA AGG GGA AGG GTG GGA AGA AAC CCA CAA

AAA GAG AAT GAT CAA TAC ATC TTT ACC GGA

CAA CCT CTG AAC AAT GAC GAG GAC CAC GCT

CAT TGG ACA GAA GCA AAA ATG TTG CTC GAC

AAC ATT AAT ACT CCT GAG GGC ATT ATC CCC

GCC CTC TTT GAA CCT GAG AGA GAA AAG AGT

GCA GCT ATC GAC GGA GAG TAC AGG TTA AAA

GGA GAG TCA AGA AAA ACC TTC GTT GAA CTC

ATG AGG AGA GGC GAC TTA CCA GTC TGG TTG

GCA CAC AAA GTG GCC AGT GAA GGG ATA AAG

TAC ACA GAC AGA AAA TGG TGT TTC GAT GGA

CAA AGA AAC AAT CAG ATA CTC GAA GAG AAC

ATG GAC GTG GAA ATC TGG ACA AAA GAG GGA

GAA AAG AAA AAG CTA AGA CCA AGA TGG CTT

GAC GCT AGA ACA TAC TCA GAC CCT CTC GCC

CTT AAA GAA TTT AAA GAT TTT GCA GCT GGA

AGA AAA TCC ATA GCA CTC GAT CTG GTC ACA

GAG ATA GGG AGG GTC CCA TCA CAT TTG GCA

CAT AGG ACC AGA AAC GCT CTT GAT AAT CTG

GTG ATG CTA CAC ACC TCA GAA GAT GGA GGG

AGG GCA TAC AGG CAC GCC GTT GAG GAA TTA

CCC GAA ACA ATG GAA ACC CTC TTA CTG CTA

GGA TTA ATG ATA CTA CTG ACA GGC GGA GCA

ATG TTG TTT TTG ATT TCA GGG AAA GGA ATA

GGA AAA ACA AGC ATA GGG CTT ATA TGT GTG

ATA GCC TCA TCT GGA ATG CTT TGG ATG GCA

GAG GTG CCA CTA CAA TGG ATA GCC TCA GCA

ATC GTG CTT GAA TTT TTC ATG ATG GTG CTG

TTG ATA CCT GAA CCA GAA AAG CAA AGA ACA

CCA CAA GAC AAC CAA TTA GCA TAC GTT GTC

ATC GGA ATA TTG ACA TTA GCC GCA ACT ATA

GCC GCA AAC GAG ATG GGG CTA CTG GAG ACA

ACT AAA AGA GAT CTT GGG ATG TCC AAA GAA

CCA GGG GTT GTC TCC CCT ACA TCT TAC CTA

GAT GTG GAC CTA CAT CCT GCA TCT GCA TGG

ACA CTC TAT GCC GTT GCA ACC ACA GTG ATT

ACC CCA ATG TTA AGG CAT ACC ATC GAA AAC

TCT ACA GCC AAC GTG TCC CTA GCT GCA ATC

GCA AAC CAG GCC GTT GTG CTG ATG GGA TTA

GAT AAG GGC TGG CCA ATT AGT AAA ATG GAT

CTC GGA GTC CCC TTG CTA GCC CTG GGA TGC

TAT TCT CAG GTT AAT CCA TTA ACA TTA ACA

GCC GCT GTT CTC CTA CTT ATA ACC CAC TAC

GCA ATC ATA GGA CCA GGC CTG CAA GCC AAG

GCT ACA AGA GAA GCT CAA AAG AGA ACA GCA

GCT GGA ATT ATG AAA AAC CCA ACA GTG GAC

GGA ATC ATG ACA ATA GAT TTG GAT TCA GTC

ATT TTC GAT AGT AAA TTT GAG AAA CAG CTG

GGA CAA GTG ATG TTA CTC GTG CTT TGC GCT

GTT CAA CTT TTG CTA ATG AGA ACA TCC TGG

GCT TTG TGC GAG GCA TTA ACA TTG GCA ACT

GGA CCC ATA ACT ACA CTG TGG GAA GGA TCC

CCT GGG AAG TTC TGG AAC ACA ACC ATA GCA

GTG TCA ATG GCA AAC ATT TTT AGG GGA TCC

TAC TTG GCA GGA GCT GGA CTG GCC TTC TCC

ATT ATG AAA TCC GTT GGA ACC GGC AAA AGA

GGA ACC GGA TCA CAA GGA GAA ACA TTA GGA

GAA AAG TGG AAA AAG AAA TTG AAC CAA CTC

TCA AGA AAA GAA TTC GAT TTG TAT AAA AAG

TCA GGA ATT ACA GAA GTG GAC AGA ACA GAG

GCA AAG GAA GGC CTC AAG AGA GGA GAA ACA

ACT CAC CAT GCT GTC TCA AGG GGA TCA GCA

AAG TTA CAA TGG TTT GTC GAA AGA AAC ATG

GTG GTT CCA GAA GGC AGA GTG ATT GAC TTG

GGG TGT GGC AGA GGA GGG TGG TCA TAT TAC

TGC GCA GGG CTG AAA AAG GTG ACA GAG GTG

AGA GGA TAT ACA AAA GGA GGG CCA GGA CAT

GAA GAG CCA GTG CCA ATG TCC ACA TAC GGA

TGG AAC ATA GTC AAG TTA ATG AGT GGA AAA

GAC GTC TTC TAC CTA CCA CCC GAG AAA TGT

GAC ACC CTA CTT TGT GAT ATT GGC GAA TCA

TCT CCA TCT CCA ACA GTG GAA GAG AGC AGA

ACA ATT AGA GTG CTT AAA ATG GTG GAG CCA

TGG CTA AAA AAT AAC CAA TTT TGC ATT AAA

GTC CTT AAT CCA TAC ATG CCA ACA GTG ATC

GAA CAC CTG GAG AGG TTG CAG AGG AAA CAT

GGA GGC ATG CTT GTG AGA AAC CCA CTG TCA

AGA AAC AGC ACT CAT GAA ATG TAC TGG ATT

TCA AAC GGA ACA GGA AAC ATT GTT TCA AGC

GTT AAC ATG GTG TCC AGA CTG TTA CTT AAT

AGA TTT ACA ATG ACA CAC AGA AGG CCC ACA

ATT GAA AAA GAC GTG GAC CTA GGA GCC GGA

ACC AGA CAC GTG AAC GCA GAG CCC GAA ACA

CCT AAT ATG GAT GTG ATA GGG GAG AGG ATA

AAA AGA ATC AAA GAA GAG CAC AAC TCA ACA

TGG CAT TAT GAC GAT GAA AAC CCT TAT AAA

ACT TGG GCC TAC CAC GGA AGT TAT GAA GTT

AAG GCA ACA GGC AGT GCC TCT TCC ATG ATT

AAC GGA GTG GTT AAA TTG CTC ACT AAA CCT

TGG GAC GTG GTT CCA ATG GTT ACA CAA ATG

GCT ATG ACA GAC ACA ACC CCC TTT GGA CAA

CAG AGG GTC TTC AAG GAA AAG GTC GAC ACT

AGG ACC CCC AGA CCA ATG CCA GGA ACA AGG

AAA GCA ATG GAA ATA ACA GCA GAA TGG TTG

TGG AGA ACC CTG GGA AGA AAT AAA AGA CCA

AGG TTA TGC ACC AGA GAG GAA TTC ACA AAG

AAA GTG AGA ACA AAC GCA GCT ATG GGA GCA

GTG TTC ACA GAA GAG AAC CAA TGG GAC TCA

GCA AAA GCA GCC GTG GAA GAT GAA GAG TTT

TGG AAA CTC GTG GAT AGA GAA AGA GAA CTT

CAT AAA CTG GGA AAA TGT GGC TCA TGT GTG

TAC AAT ATG ATG GGA AAG AGA GAG AAG AAA

CTC GGA GAA TTC GGC AAG GCT AAA GGG TCA

AGG GCC ATT TGG TAT ATG TGG TTA GGG GCC

AGA TAC TTG GAA TTC GAG GCT CTA GGA TTT

CTT AAC GAA GAC CAC TGG TTC AGT AGG GAG

AAC TCA TAC TCA GGA GTG GAA GGA GAG GGA

CTG CAT AAA CTC GGA TAC ATT CTT AGA GAC

ATA AGT AAG ATA CCA GGA GGG GCC ATG TAC

GCA GAC GAT ACT GCA GGG TGG GAT ACA AGA

ATT ACA GAA GAC GAT CTG CAT AAC GAG GAA

AAA ATA ATC CAA CAG ATG GAC CCC GAA CAC

AGA CAA CTT GCT AAC GCC ATT TTT AAA CTA

ACT TAC CAA AAC AAG GTG GTC AAA GTC CAA

AGA CCA ACC CCT ACA GGA ACC GTT ATG GAC

ATA ATT TCC AGA AAG GAT CAG AGA GGC TCT

GGA CAA TTG GGA ACA TAC GGC TTG AAT ACC

TTT ACA AAC ATG GAA GCA CAA CTT GTG AGA

CAA ATG GAA GGG GAA GGG GTG CTG ACA AAA

GCT GAC TTG GAA AAC CCA CAT TTA CTA GAG

AAG AAA ATC ACA CAA TGG TTG GAA ACA AAG

GGA GTG GAG AGA CTG AAG AGA ATG GCT ATA

TCA GGC GAT GAC TGT GTG GTT AAG CCT ATA

GAC GAT AGG TTT GCC AAT GCA CTG CTC GCC

CTG AAT GAC ATG GGA AAG GTG AGA AAG GAT

ATT CCA CAA TGG CAA CCA TCA AAA GGG TGG

CAC GAT TGG CAA CAG GTG CCA TTT TGT AGC

CAC CAT TTC CAT GAG CTG ATA ATG AAA GAC

GGC AGA AAA TTA GTG GTC CCT TGT AGA CCA

CAA GAT GAG CTC ATA GGG AGA GCC AGA ATT

AGC CAG GGA GCC GGC TGG TCT CTG AGA GAG

ACT GCA TGT CTG GGA AAA GCC TAC GCA CAA

ATG TGG TCA CTG ATG TAT TTC CAC AGA AGG

GAC CTC AGA TTA GCA TCC AAT GCA ATA TGC

AGC GCA GTG CCA GTG CAC TGG GTG CCA ACA

TCA AGA ACA ACT TGG TCC ATA CAC GCC CAC

CAT CAA TGG ATG ACA ACA GAG GAC ATG CTG

ACA GTG TGG AAT AGA GTG TGG ATT GAA GAG

AAT CCA TGG ATG GAA GAT AAA ACA CCA GTC

ACA ACA TGG GAA AAC GTG CCA TAC TTG GGG

AAA AGA GAG GAT CAA TGG TGC GGA TCA CTC

ATA GGC TTG ACA TCC AGA GCA ACA TGG GCA

CAA AAC ATC CCA ACA GCC ATA CAG CAA GTG

AGA TCA CTT ATC GGG AAT GAG GAA TTT CTC

GAC TAC ATG CCC AGC ATG AAG AGA TTC AGA

AAG GAG GAA GAG TCT GAG GGG GCC ATT TGG

TAA

60.06%
 * *GC% Content for gene (codon optimized): **

-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**
 * ( link to DNA Works output text file - **that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences):**

[] [] [] []