Protein-tyrosine-phosphatase 

 ***Protein ID (NP or XP #) or Wolbachia#:** RefSeq YP_402621.2  ***Organism (including strain):** Shigella dysenteriae Sd197 (strain: Sd197, serotype: 1)  **Etiologic Risk Group (see link below):** Risk Group 2 (RG2) - Bacterial Agents Including Chlamydia  ***Background/Disease Information (sort of like the Intro to your Mini Research Write up):**  Shigella dysenteriae infection: Shigella dysenteriae is a species of bacteria from the Shigella genus. Dysenteriae is the most common cause of epidemic dysentery in condensed populations such as refugee camps. Infection usually occurs through the fecal-oral route. Infection can be transmitted between people unless appropriate hygiene measures are undertaken. Further, Shigella dysenteriae is associated with the development of Hemolytic uremic syndrome, which includes anemia, thrombocytopenia, and renal failure.
 * Target : **phosphotyrosine-protein phosphatase
 * *NCBI Gene ID: ** 3798734
 * *RefSeq#: **VBIShiDys99784_1106

 **Essentiality of this protein:** []

Information about this protein and gene: []  []   **Complex of proteins?:** N/A <span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Druggable Target:** <span style="font-family: Arial,sans-serif; font-size: 10pt;">protein tyrosine phosphate + H2O = protein tyrosine + phosphate <span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Link to BRENDA EC# page:** <span style="font-family: Arial,sans-serif; font-size: 10pt;">[] <span style="font-family: Arial,sans-serif; font-size: 10pt;">However, Protein-tyrosine-phosphatase<span style="font-family: Arial,sans-serif; font-size: 10pt;"> of only humans and other species were found. No information about this typical organism was found.
 * <span style="background-color: white; font-family: Arial,sans-serif; font-size: 10pt;">EC#: **<span style="background-color: white; font-family: Arial,sans-serif; font-size: 10pt;"> 3.1.3.48



<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Enzyme Assay information (spectro** **<span style="font-family: Arial,sans-serif; font-size: 10pt; line-height: 1.5;">photometric, coupled assay ?, reagents): ** <span style="font-family: Georgia,serif; font-size: 11.5pt;">Tyrosine phosphorylation and dephosphorylation of proteins play a critical role during many processes of the immune system, from early development to fully differentiated effector function. Since the opposing actions of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs) determine the steady state level of tyrosine phosphorylation on a given protein, it is often important for mechanistic studies to determine the specific activities of PTKs and PTPs. Dephosphorylation of the chromogenic substrate pNPP generates p-nitrophenol, which at an alkaline pH has an intense yellow color that can be measured at 405 nm using a spectrophotometer (Read absorbance at 405 nm). [] __Reagents and Solutions:__

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<span style="font-family: 'Times New Roman',serif; font-size: 12pt;">No information from Sigma about reagents costs.
 * <span style="background-color: white; font-family: Arial,sans-serif; font-size: 10pt;">-- link to Sigma (or other company) page for assay or assay reagents (substrates) **

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<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **-- List cost and quantity of substrate reagents and supplier** <span style="font-family: Arial,sans-serif; font-size: 10pt;"> **The experimenters already have some in lab at UT. However, there was no information on the website about the reagents and suppliers.**

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Structure Available (Using Homology Model):**

Query Coverage: 98% Max Score:198 Max Ident: 62% Chain used for homology: Chain A, Crystal Structure Of The Tyrosine Phosphatase Wzb From Escherichia Coli K30 In Complex With Sulphate


 * Image of homology protein (PyMol Chain A showing, everything else were hidden): **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Current Inhibitors:** Antipain, Aprotinin, Leupeptin, Pepstatin A, and PMSF

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Expression Information (has it been expressed in bacterial cells):** <span style="font-family: Arial,sans-serif; font-size: 10pt;">It had been expressed in E-Coli bacteria and human.

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **Purification Method:**

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> N/A


 * <span style="background-color: white; font-family: Arial,sans-serif; font-size: 10pt;">*Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **

<span style="font-family: Arial,sans-serif;">MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGLVKHPADATAADVAANH <span style="font-family: Arial,sans-serif;">GVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVRGKTMLFGQWLEQKEIPDP <span style="font-family: Arial,sans-serif;">YRKSQDAFEHVYGMLERASQEWAKRLSR

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** *length of your protein in Amino Acids: ** 148

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** Molecular Weight of your protein in kiloDaltons using the ** [|**Expasy ProtParam**]**<span style="background-color: white; font-family: Arial,sans-serif; font-size: 10pt;">website: ** <span style="font-family: Arial,sans-serif; font-size: 10pt;"> 16.385kDA

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** Molar Extinction coefficient of your protein at 280 nm wavelength: ** <span style="font-family: Arial,sans-serif;">Ext. coefficient 15595

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 * <span style="background-color: white; font-family: Arial,sans-serif; font-size: 11pt;">TMpred graph Image **<span style="background-color: white; font-family: Arial,sans-serif; font-size: 11pt;"> ( <span style="font-family: Arial,sans-serif; font-size: 11pt;">@http://www.ch.embnet.org/software/TMPRED_form.html <span style="background-color: white; font-family: Arial,sans-serif; font-size: 11pt;">). Input your amino acid sequence to it.

code ATGGCCCAACTAAAATTTAACTCAATCCTGGTAGTTTGTACCGGCAACATTTGCCGTTCGCCCATTGGCG code AGCGCTTGCTGCGTAAACGTCTGCCAGGCGTAAAAGTCAAATCGGCAGGTGTTCATGGTCTGGTAAAACA CCCTGCAGATGCGACAGCAGCAGATGTTGCCGCTAATCATGGCGTATCTCTTGAAGGACATGCCGGACGT AAGCTCACTGCTGAGATGGCACGAAATTACGATCTGATTCTGGCAATGGAGTCGGAACATATTGCTCAGG TTACCGCAATCGCTCCCGAGGTCCGCGGGAAGACAATGCTGTTTGGGCAATGGCTGGAACAGAAAGAGAT CCCGGATCCCTATCGTAAAAGTCAGGACGCATTTGAACATGTCTACGGTATGTTGGAGCGCGCCAGTCAG GAATGGGCGAAGCGCCTCAGCCGGTAA
 * <span style="background-color: white; font-family: Arial,sans-serif; font-size: 11pt;">*CDS Gene Sequence (paste as text only): **

<span style="font-family: Arial,sans-serif; font-size: 11pt;"> ** *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): ** <span style="font-family: Arial,sans-serif; font-size: 11pt;"> ** *GC% Content for gene (codon optimized): **
 * <span style="background-color: white; font-family: Arial,sans-serif; font-size: 11pt;">*GC% Content for gene: 51.9% **