Thioredoxin+reductase+(NADPH)+Entamoeba+Histolytica.

**Thiredoxin-disulfide Reductase**

 * NCBI Gene 32287584**


 * Protein ID YP_009361307.1**


 * Organism (including strain): Mycobacterium bovis AF2122/97**
 * Etiologic Risk Group:**


 * Disease Information: Myobacterium bovis is causing agent for Mycobacterium turberculosis, which is usually transmitted to humans by consuming raw or infected cows milk. It is a chronic bacterial disease of cattle that can affect other species of animals in which evolved from Africa. **

https://www.ncbi.nlm.nih.gov/gene/32287584

Is it a monomer or multimer as biological unit**?** multimer https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1432-1327.2000.01703.x
 * Essentiality of this protein:**
 * Complex of proteins?:** 2 amino acid chants and 6 ligand in ASU
 * Druggable Target**

@https://www.brenda-enzymes.org/enzyme.php?ecno=1.8.1.9&Suchword=&reference=&UniProtAcc=&organism%5B%5D=Mycobacterium+tuberculosis
 * EC#: 1.8.1.9**
 * Link to BRENDA EC# page:**

Show screenshot of BRENDA enzyme mechanism schematic

@https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Enzyme_Assay/thioredoxinred.pdf
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**

PDB #: 2A87
 * Structure (PDB or Homology model)**




 * Current Inhibitors:** Carmustine (BCNU) and other nitrosoureas, Cisplatin, Anthracyclines, Azelaic acid, 13-cis-Retinoic acid, 1-Chloro-2,4-dinitrobenzene, Auranofin and aurothioglucose
 * Expression Information:** Expression of wild-type fusion protein and truncated mutant in E. coli
 * Purification Method :** recombinant wild-type fusion protein and truncated mutant

A87:A|PDBID|CHAIN|SEQUENCE
 * Amino Acid Sequence**

MTAPPVHDRAHHPVRDVIVIGSGPAGYTAALYAARAQLAPLVFEGTSFGGALMTTTDVENYPGFRNGITGPELMDEMREQ

ALRFGADLRMEDVESVSLHGPLKSVVTADGQTHRARAVILAMGAAARYLQVPGEQELLGRGVSSCATCDGFFFRDQDIAV

IGGGDSAMEEATFLTRFARSVTLVHRRDEFRASKIMLDRARNNDKIRFLTNHTVVAVDGDTTVTGLRVRDTNTGAETTLP

VTGVFVAIGHEPRSGLVREAIDVDPDGYVLVQGRTTSTSLPGVFAAGDLVDRTYRQAVTAAGSGCAAAIDAERWLAEHAA

TGEADSTDALIGAQR

>2A87:B|PDBID|CHAIN|SEQUENCE

MTAPPVHDRAHHPVRDVIVIGSGPAGYTAALYAARAQLAPLVFEGTSFGGALMTTTDVENYPGFRNGITGPELMDEMREQ

ALRFGADLRMEDVESVSLHGPLKSVVTADGQTHRARAVILAMGAAARYLQVPGEQELLGRGVSSCATCDGFFFRDQDIAV

IGGGDSAMEEATFLTRFARSVTLVHRRDEFRASKIMLDRARNNDKIRFLTNHTVVAVDGDTTVTGLRVRDTNTGAETTLP

VTGVFVAIGHEPRSGLVREAIDVDPDGYVLVQGRTTSTSLPGVFAAGDLVDRTYRQAVTAAGSGCAAAIDAERWLAEHAA

TGEADSTDALIGAQR
 * length of your protein in Amino Acids: 710**
 * Molecular Weight: 75723.05 **
 * Molar Extinction coefficient of your protein at 280 nm wavelength: 29505**
 * TMpred graph Image**

code format="genbank"  1..335 /gene="trxB2" /locus_tag="BQ2027_MB3944" /coded_by="NC_002945.4:4340100..4341107" /experiment="experimental evidence, no additional details                    recorded" /note="Mb3944, trxB2, len: 335 aa. Equivalent to Rv3913,                    len: 335 aa, from Mycobacterium tuberculosis strain H37Rv,                     (99.7% identity in 335 aa overlap). Probable trxB2,                     thioredoxin reductase (EC 1.6.4.5) (see citation below),                     equivalent to O30973|TRXB_MYCSM THIOREDOXIN REDUCTASE from                     Mycobacterium smegmatis (311 aa), FASTA scores: opt: 1575,                     E: 1.8e-87, (78.35% identity in 305 aa overlap); and                     highly similar, but shorter at C-terminus, to                     P46843|TRXB_MYCLE|TRXB/A|TRX|ML2703 BIFUNCTIONAL                     THIOREDOXIN REDUCTASE/THIOREDOXIN from Mycobacterium                     leprae (458 aa), FASTA scores: opt: 1766, E: 8.7e-99,                     (83.25% identity in 328 aa overlap). Also highly similar                     to many e.g. P52215|TRXB_STRCO|SCH24.12 from Streptomyces coelicolor (321 aa), FASTA scores: opt: 1249, E: 7.2e-68, (60.4% identity in 313 aa overlap); Q9Z8M4|TRXB_CHLPN from Chlamydia pneumoniae (Chlamydophila                    pneumoniae) (311 aa), FASTA scores: opt: 978, E: 1.3e-51, (49.85% identity in 307 aa overlap); P09625|TRXB_ECOLI|B0888 from Escherichia coli strain K12 (320 aa), FASTA scores: opt: 948, E: 8.6e-50, (49.2%                    identity in 309 aa overlap); etc. Contains PS00573 Pyridine nucleotide-disulphide oxidoreductases class-II active site. BELONGS TO THE PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASES CLASS-II. COFACTOR: FAD (BY SIMILARITY). Protein product from Mb3944 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3944 found to                    be expressed during exponential growth in Sauton's minimal media by RNA-sequencing."                    /transl_table= [|11]                      /db_xref="GeneID: [|32287584] "  code
 * CDS Gene Sequence **
 * GC% Content for gene: 10.848401**


 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**
 * ( link to DNA Works output text file - **that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences):**