GlcB+malate+synthase,+Mycobacterium+leprae+.

**Organism:** Mycobacterium leprae (strain Br4923)
 * Target (protein/gene name): ** GlcB, malate synthase G
 * E.C Number: ** 2.3.3.9
 * NCBI Gene # or RefSeq#: ** NC_002677.1
 * PBD ID: **4EX4
 * Etiologic Risk Group: **n/a
 * Disease Information: **//Mycobacterium Leprae// is a slow-growing bacteria that causes Leprosy, which is also known as Hansen's Disease. Leprosy is a human disease that affects the skin, peripheral nerves, eyes, and mucosa of the upper respiratory tract. The bacteria causes numbness, nerve damage, and gradual tissue decay and disfigurement of the limbs. The nerve damage from the disease can lead paralysis and crippled hands and feet. The signs and symptoms of leprosy mainly affect the skin, nerves, and mucous membranes. Some signs are discolored skin patches, loss of eyebrow hair or eyelashes, muscle weakness or paralysis, enlarged nerves, etc.

Links: 1.[] 2. []


 * Essentiality: ** Malate synthase catalyzes the irreversible condensation of acetyl-CoA with glyoxylate which forms (S)-malate. Also, the enzyme plays a role in the glyoxylate cycle, which leads to the loss of CO(2).

1. []


 * Druggable Target: ** Not available


 * Assayable Enzyme Target: ** []

Assayed by adding cell extracts to H2O2 in K2HPO4. O-dianisidine was used for peroxidase and was added to a phosphate buffer and H2O2 in deionized water.


 * Materials and Reagents: **

The enzyme catalyses the irreversible condensation of acetyl-CoA with glyoxylate to form (S)-malate. Among other functions, the enzyme participates in the glyoxylate cycle, a modified version of the TCA cycle that bypasses steps that lead to a loss of CO2.

Link to Brenda: []


 * Structure: **


 * Image of Protein: **


 * Fig 1. ** Structure of GlcB from Mycobacterium leprae

code Query 1       ATGACTGATCGCGTGTCGGCGGGGAATTTGCGCGTCGCCCGTGTGCTTTACGACTTCGTC  60 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110146  ATGACTGATCGCGTGTCGGCGGGGAATTTGCGCGTCGCCCGTGTGCTTTACGACTTCGTC  110205
 * Pairwise alignment of BlastP: **

Query 61      AACAACGAAGCCCTGCCTGGCACCGACATCAACCCAAACAGCTTCTGGTCGGGCGTCGCC  120 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110206  AACAACGAAGCCCTGCCTGGCACCGACATCAACCCAAACAGCTTCTGGTCGGGCGTCGCC  110265

Query 121     AAGGTCGTCGCCGACCTCACCCCGCAAAACCAAAGTTTGCTGAACTCCCGCGATGAGCTG  180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110266  AAGGTCGTCGCCGACCTCACCCCGCAAAACCAAAGTTTGCTGAACTCCCGCGATGAGCTG  110325

Query 181     CAGGCCCAGATTGACAAATGGCACCGCCATCGTGTGATCGAGCCATTCGACGTGGATGCT  240 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110326  CAGGCCCAGATTGACAAATGGCACCGCCATCGTGTGATCGAGCCATTCGACGTGGATGCT  110385

Query 241     TATCGGCAGTTCCTCATCGATATCGGCTATCTGCTGCCTGAACCCGATGACTTCACCATC  300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110386  TATCGGCAGTTCCTCATCGATATCGGCTATCTGCTGCCTGAACCCGATGACTTCACCATC  110445

Query 301     AGCACCTCAGGAGTAGACGACGAGATCACCATGACCGCCGGCCCGCAGCTGGTGGTGCCG  360 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110446  AGCACCTCAGGAGTAGACGACGAGATCACCATGACCGCCGGCCCGCAGCTGGTGGTGCCG  110505

Query 361     GTACTTAACGCACGGTTCGCACTCAACGCCGCCAACGCCCGCTGGGGCTCCCTTTACGAC  420 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110506  GTACTTAACGCACGGTTCGCACTCAACGCCGCCAACGCCCGCTGGGGCTCCCTTTACGAC  110565

Query 421     GCTCTATATGGCACCGACACCATACCGGAAACCGAGGGAGCCGAAAAGGGCTCTGAGTAC  480 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110566  GCTCTATATGGCACCGACACCATACCGGAAACCGAGGGAGCCGAAAAGGGCTCTGAGTAC  110625

Query 481     AACAAGATTCGCGGCGACAAGGTGATCGCCTACGCACGCAAGTTTATGGATCAGGCCGTC  540 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110626  AACAAGATTCGCGGCGACAAGGTGATCGCCTACGCACGCAAGTTTATGGATCAGGCCGTC  110685

Query 541     CCGCTGGCTTCTGACTCATGGACTAACGCTACGGGAGTGAGCATCTTCGACGGCCAGCTT  600 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110686  CCGCTGGCTTCTGACTCATGGACTAACGCTACGGGAGTGAGCATCTTCGACGGCCAGCTT  110745

Query 601     CAGATCGCCATCGGCACGAATTCCACCGGATTAGCCAGTCCCGAAAAGTTTGTCGGCTAC  660 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110746  CAGATCGCCATCGGCACGAATTCCACCGGATTAGCCAGTCCCGAAAAGTTTGTCGGCTAC  110805

Query 661     AATCGCCAGTTGCGATCCTCCAACTGGTCGGTGCTGTTGGCCAATCACGGCCTGCACATC  720 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110806  AATCGCCAGTTGCGATCCTCCAACTGGTCGGTGCTGTTGGCCAATCACGGCCTGCACATC  110865

Query 721     GAGGTCTTGATCGACCCGGAATCACCAATAGGCAAGACTGACCCCGTCGGCATCAAGGAT  780 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110866  GAGGTCTTGATCGACCCGGAATCACCAATAGGCAAGACTGACCCCGTCGGCATCAAGGAT  110925

Query 781     GTGATCCTGGAATCGGCCATCACCACGATCATGGACTTCGAAGACTCAGTCACTGCCGTC  840 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110926  GTGATCCTGGAATCGGCCATCACCACGATCATGGACTTCGAAGACTCAGTCACTGCCGTC  110985

Query 841     GACGCCGACGACAAGGTACGCGGCTACCGCAACTGGCTCGGCCTGAACAAAGGTGACTTA  900 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 110986  GACGCCGACGACAAGGTACGCGGCTACCGCAACTGGCTCGGCCTGAACAAAGGTGACTTA  111045

Query 901     ACCGAGGAAGTCAACAAGGACGGCAAGACCTTCACCCGCGTGCTCAACGCCGACCGCAGC  960 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111046  ACCGAGGAAGTCAACAAGGACGGCAAGACCTTCACCCGCGTGCTCAACGCCGACCGCAGC  111105

Query 961     TACACCACGCCCGACGGCGGTGAATTGACCCTGCCCGGGCGCAGCCTGTTGTTCGTCCGC  1020 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111106  TACACCACGCCCGACGGCGGTGAATTGACCCTGCCCGGGCGCAGCCTGTTGTTCGTCCGC  111165

Query 1021    AACGTGGGCCATCTGACGACCAGCGACGCGATTCTCGTGGACGGAGGTGACGGTCAGGAA  1080 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111166  AACGTGGGCCATCTGACGACCAGCGACGCGATTCTCGTGGACGGAGGTGACGGTCAGGAA  111225

Query 1081    AAAGAAGTGTTCGAGGGCATCATAGATGCAGTGTTCACCGGTCTGGCCGCAATCCACGGA  1140 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111226  AAAGAAGTGTTCGAGGGCATCATAGATGCAGTGTTCACCGGTCTGGCCGCAATCCACGGA  111285

Query 1141    CTCAAAACCGGCGAAGCTAATGGCCCACTGACCAACAGCCGCACCGGTTCCATCTACATC  1200 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111286  CTCAAAACCGGCGAAGCTAATGGCCCACTGACCAACAGCCGCACCGGTTCCATCTACATC  111345

Query 1201    GTCAAGCCGAAAATGCACGGTCCCGCGGAGGTTGCGTTCACTTGCGAGCTGTTCAGCCGC  1260 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111346  GTCAAGCCGAAAATGCACGGTCCCGCGGAGGTTGCGTTCACTTGCGAGCTGTTCAGCCGC  111405

Query 1261    GTCGAAGACGTGCTGGGGTTGCCGCAGGGCACCTTGAAGGTCGGCATCATGGATGAGGAG  1320 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111406  GTCGAAGACGTGCTGGGGTTGCCGCAGGGCACCTTGAAGGTCGGCATCATGGATGAGGAG  111465

Query 1321    CGGCGTACGACACTCAATTTGAAGGCGTGCATCAAGGCCGCCGCCGATCGGGTCGTGTTT  1380 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111466  CGGCGTACGACACTCAATTTGAAGGCGTGCATCAAGGCCGCCGCCGATCGGGTCGTGTTT  111525

Query 1381    ATCAACACTGGCTTCTTGGACCGCACCGGCGACGAGATCCACACCTCAATGGAGGCCGGC  1440 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111526  ATCAACACTGGCTTCTTGGACCGCACCGGCGACGAGATCCACACCTCAATGGAGGCCGGC  111585

Query 1441    CCGATGATTCGTAAGGGTGCGATGAAAAACAGCACCTGGATCAAAGCCTACGAGGACGCC  1500 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111586  CCGATGATTCGTAAGGGTGCGATGAAAAACAGCACCTGGATCAAAGCCTACGAGGACGCC  111645

Query 1501    AATGTTGACATCGGACTAGCCGCAGGCTTCAAGGGCAAAGCACAGATCGGCAAGGGCATG  1560 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111646  AATGTTGACATCGGACTAGCCGCAGGCTTCAAGGGCAAAGCACAGATCGGCAAGGGCATG  111705

Query 1561    TGGGCAATGACCGAATTAATGGCTGATATGGTCGAGCAGAAGATCGGCCAGCCTAAGGCG  1620 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111706  TGGGCAATGACCGAATTAATGGCTGATATGGTCGAGCAGAAGATCGGCCAGCCTAAGGCG  111765

Query 1621    GGCGCCACCACCGCGTGGGTACCCTCGCCCACTGCGGCCACGCTGCATGCGATGCACTAC  1680 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111766  GGCGCCACCACCGCGTGGGTACCCTCGCCCACTGCGGCCACGCTGCATGCGATGCACTAC  111825

Query 1681    CACCAGGTCGACGTGGCTGCCGTGCAACAAGAACTGACCGGCCAGAGACGTGCCACCGTT  1740 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111826  CACCAGGTCGACGTGGCTGCCGTGCAACAAGAACTGACCGGCCAGAGACGTGCCACCGTT  111885

Query 1741    GACCAGCTGTTGACCATTCCGTTGGCCAAGAAACTGGCCTGGGCTCCCGAGGAGATCCGC  1800 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111886  GACCAGCTGTTGACCATTCCGTTGGCCAAGAAACTGGCCTGGGCTCCCGAGGAGATCCGC  111945

Query 1801    GAGGAGGTCGATAATGACTGTCAGTCCATCCTCGGTTATGTGGTGCGCTGGGTGGACCAG  1860 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 111946  GAGGAGGTCGATAATGACTGTCAGTCCATCCTCGGTTATGTGGTGCGCTGGGTGGACCAG  112005

Query 1861    GGTATCGGTTGCTCGAAGGTGCCTGACATCCACAATGTGGCACTAATGGAGGACCGCGCC  1920 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 112006  GGTATCGGTTGCTCGAAGGTGCCTGACATCCACAATGTGGCACTAATGGAGGACCGCGCC  112065

Query 1921    ACCCTGCGGATCTCGAGCCAGTTGCTAGCAAACTGGCTGCGCCACGGCGTAATCACCAGC  1980 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 112066  ACCCTGCGGATCTCGAGCCAGTTGCTAGCAAACTGGCTGCGCCACGGCGTAATCACCAGC  112125

Query 1981    GAGGACGTGCGGGCCAGCCTGGAACGGATGGCGCCGTTAGTCGATCAGCAAAACGCCGAA  2040 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 112126  GAGGACGTGCGGGCCAGCCTGGAACGGATGGCGCCGTTAGTCGATCAGCAAAACGCCGAA  112185

Query 2041    GACCCAGCATATCGCCCGATGGCGCCTAATTTCGATGACAGCATCGCGTTCCTAGCGGCG  2100 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 112186  GACCCAGCATATCGCCCGATGGCGCCTAATTTCGATGACAGCATCGCGTTCCTAGCGGCG  112245

Query 2101    CAGGAACTGATACTGTCCGGCGCACAACAGCCCAACGGCTATACCGAGCCGATCCTGCAT  2160 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 112246  CAGGAACTGATACTGTCCGGCGCACAACAGCCCAACGGCTATACCGAGCCGATCCTGCAT  112305

Query 2161    CGCCGTCGTCGCGAATTCAAGGCACAGAACCGGTGA  2196 |||||||||||||||||||||||||||||||||||| Sbjct 112306  CGCCGTCGTCGCGAATTCAAGGCACAGAACCGGTGA  112341

code Malate synthase in mycobacterium leprae is expressed and causes the disease Leprosy.
 * Query Coverage: ** 100%
 * Max % Identities: ** 100%
 * % Positives: ** 100%
 * Chain used for Homology: ** A
 * Similarity to Human: ** No significant similarity was found to show any results
 * Inhibitors: ** None
 * Expression: **
 * Transmembrane Bound Regions: ** There are 3-4 transmembrane bound regions.
 * Fig 2. ** TMPred output graph for Malate Synthase


 * Gene Available: **
 * [] **

code >NC_002677.1:2457996-2460191 Mycobacterium leprae TN chromosome, complete genome ATGACTGATCGCGTGTCGGCGGGGAATTTGCGCGTCGCCCGTGTGCTTTACGACTTCGTCAACAACGAAG CCCTGCCTGGCACCGACATCAACCCAAACAGCTTCTGGTCGGGCGTCGCCAAGGTCGTCGCCGACCTCAC CCCGCAAAACCAAAGTTTGCTGAACTCCCGCGATGAGCTGCAGGCCCAGATTGACAAATGGCACCGCCAT CGTGTGATCGAGCCATTCGACGTGGATGCTTATCGGCAGTTCCTCATCGATATCGGCTATCTGCTGCCTG AACCCGATGACTTCACCATCAGCACCTCAGGAGTAGACGACGAGATCACCATGACCGCCGGCCCGCAGCT GGTGGTGCCGGTACTTAACGCACGGTTCGCACTCAACGCCGCCAACGCCCGCTGGGGCTCCCTTTACGAC GCTCTATATGGCACCGACACCATACCGGAAACCGAGGGAGCCGAAAAGGGCTCTGAGTACAACAAGATTC GCGGCGACAAGGTGATCGCCTACGCACGCAAGTTTATGGATCAGGCCGTCCCGCTGGCTTCTGACTCATG GACTAACGCTACGGGAGTGAGCATCTTCGACGGCCAGCTTCAGATCGCCATCGGCACGAATTCCACCGGA TTAGCCAGTCCCGAAAAGTTTGTCGGCTACAATCGCCAGTTGCGATCCTCCAACTGGTCGGTGCTGTTGG CCAATCACGGCCTGCACATCGAGGTCTTGATCGACCCGGAATCACCAATAGGCAAGACTGACCCCGTCGG CATCAAGGATGTGATCCTGGAATCGGCCATCACCACGATCATGGACTTCGAAGACTCAGTCACTGCCGTC GACGCCGACGACAAGGTACGCGGCTACCGCAACTGGCTCGGCCTGAACAAAGGTGACTTAACCGAGGAAG TCAACAAGGACGGCAAGACCTTCACCCGCGTGCTCAACGCCGACCGCAGCTACACCACGCCCGACGGCGG TGAATTGACCCTGCCCGGGCGCAGCCTGTTGTTCGTCCGCAACGTGGGCCATCTGACGACCAGCGACGCG ATTCTCGTGGACGGAGGTGACGGTCAGGAAAAAGAAGTGTTCGAGGGCATCATAGATGCAGTGTTCACCG GTCTGGCCGCAATCCACGGACTCAAAACCGGCGAAGCTAATGGCCCACTGACCAACAGCCGCACCGGTTC CATCTACATCGTCAAGCCGAAAATGCACGGTCCCGCGGAGGTTGCGTTCACTTGCGAGCTGTTCAGCCGC GTCGAAGACGTGCTGGGGTTGCCGCAGGGCACCTTGAAGGTCGGCATCATGGATGAGGAGCGGCGTACGA CACTCAATTTGAAGGCGTGCATCAAGGCCGCCGCCGATCGGGTCGTGTTTATCAACACTGGCTTCTTGGA CCGCACCGGCGACGAGATCCACACCTCAATGGAGGCCGGCCCGATGATTCGTAAGGGTGCGATGAAAAAC AGCACCTGGATCAAAGCCTACGAGGACGCCAATGTTGACATCGGACTAGCCGCAGGCTTCAAGGGCAAAG CACAGATCGGCAAGGGCATGTGGGCAATGACCGAATTAATGGCTGATATGGTCGAGCAGAAGATCGGCCA GCCTAAGGCGGGCGCCACCACCGCGTGGGTACCCTCGCCCACTGCGGCCACGCTGCATGCGATGCACTAC CACCAGGTCGACGTGGCTGCCGTGCAACAAGAACTGACCGGCCAGAGACGTGCCACCGTTGACCAGCTGT TGACCATTCCGTTGGCCAAGAAACTGGCCTGGGCTCCCGAGGAGATCCGCGAGGAGGTCGATAATGACTG TCAGTCCATCCTCGGTTATGTGGTGCGCTGGGTGGACCAGGGTATCGGTTGCTCGAAGGTGCCTGACATC CACAATGTGGCACTAATGGAGGACCGCGCCACCCTGCGGATCTCGAGCCAGTTGCTAGCAAACTGGCTGC GCCACGGCGTAATCACCAGCGAGGACGTGCGGGCCAGCCTGGAACGGATGGCGCCGTTAGTCGATCAGCA AAACGCCGAAGACCCAGCATATCGCCCGATGGCGCCTAATTTCGATGACAGCATCGCGTTCCTAGCGGCG CAGGAACTGATACTGTCCGGCGCACAACAGCCCAACGGCTATACCGAGCCGATCCTGCATCGCCGTCGTC GCGAATTCAAGGCACAGAACCGGTGA code
 * Gene Sequence: **