dihidrofolate+reductase+(T.+cruzi)


 * *Target (protein/gene name): **

 ** *NCBI Gene # or RefSeq#: **

 ** *Protein ID (NP or XP #) or Wolbachia#: **

 ** *Organism (including strain): **

 ** Etiologic Risk Group (see link below): **

 ** *Background/Disease Information (sort of like the Intro to your Mini Research Write up): **

 ** Essentiality of this protein: **

 ** Complex of proteins?: **

 ** Druggable Target: **

 ** *EC#: **

 ** Link to BRENDA EC# page: **

 ** -- ** Show screenshot of BRENDA enzyme mechanism schematic

 ** Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **

 ** -- link to Sigma (or other company) page for assay or assay reagents (substrates) **

 ** -- link (or citation) to paper that contains assay information **

 ** -- List cost and quantity of substrate reagents and supplier **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** Structure Available (PDB or Homology model) **

<span style="font-family: Arial,sans-serif; font-size: 10pt;">-- PDB # or closest PDB entry if using homology model:

<span style="font-family: Arial,sans-serif; font-size: 10pt;">-- For Homology Model option:

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> Show pairwise alignment of your BLASTP search in NCBI against the PDB

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> Query Coverage:

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> Max % Identities:

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> % Positives

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> Chain used for homology:

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** Current Inhibitors: **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** Expression Information (has it been expressed in bacterial cells): **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** Purification Method: **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** Image of protein (PyMol with features delineated and shown separately): **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** *length of your protein in Amino Acids **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** Molecular Weight of your protein in kiloDaltons using the ** <span class="apple-converted-space" style="font-family: Arial,sans-serif; font-size: 10pt;"> <span style="font-family: Arial,sans-serif; font-size: 10pt;">** [|Expasy ProtParam] ** <span class="apple-converted-space" style="font-family: Arial,sans-serif; font-size: 10pt;"> <span style="font-family: Arial,sans-serif; font-size: 10pt;">** website **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** Molar Extinction coefficient of your protein at 280 nm wavelength: **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** TMpred graph Image ** ( <span style="font-family: Arial,sans-serif; font-size: 10pt;">@http://www.ch.embnet.org/software/TMPRED_form.html <span style="background-color: white; font-family: Arial,sans-serif; font-size: 10pt;">). Input your amino acid sequence to it.

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** *CDS Gene Sequence (paste as text only): **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** *GC% Content for gene: **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** *GC% Content for gene (codon optimized): **

<span style="font-family: Arial,sans-serif; font-size: 10pt;">Do Not Need this info for Spring (but still copy these lines to your Target page for now)

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** ( ** **<span style="color: green; font-family: Arial,sans-serif; font-size: 10pt;">link to DNA Works output text file - ** <span style="background-color: white; font-family: Arial,sans-serif; font-size: 10pt;">that should be saved in your Google Docs folder after you did the primer design protocol)

<span style="font-family: Arial,sans-serif; font-size: 10pt;">-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> ** Primer design results for 'tail' primers (this is just 2 sequences): **