Protein+Labs+Sp12

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**__Online Report: __** **__Lab Report: __** This report will encompass the last three (3) labs: protein expression, purification, and characterization. 1,000 words total – Since this is online – you must be efficient with your words! Make them count! **Title: **think of something semi-creative **Intro words: ** this should have some background information on technique of protein production and purification. There is a good article here: Nat Methods. **2008** Feb;5(2):135-46. Protein production and purification. Also a good website: []  End with an objective statement where you briefly sum-up what you set out to do in this set of experiments. Include a brief hypothesis. **M&M 250 words **: be __succinct__, __concise__, __and brief__ while allowing an informed reader to be able to replicate your work. Do not show calculations for prelabs. **Results **: show images of your results that you have already taken **<span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;"> CAPTIONS **<span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;">: make sure you have good captions (captions don’t count against the word limit) **<span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;">Transformation plates images - **<span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;"> Mention how many colonies you got. **Flask images, purple pellet images (include the weight of your pellet)** **<span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;">Images of Elution 1 and 2 in tubes, Nanodrop screen shots - **<span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;">Include yields from your Nanodrop spectrophotometry for your protein amounts. **<span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;">Show brief calculations for Beer’s law calculations. A=Ebc ** **<span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;">Protein Gel images **<span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;">, **Image of Ladder from web as reference** (molecular weight marker used – get from website of company) **<span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;">Discusssion ** <span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;">Analyze your results, address sources of error, and answer any questions from the handout **<span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;">Conclusions ** <span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;"> Recap what was done <span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;"> State key finding(s) <span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;"> Address future directions/applications of this work to VDS research **<span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;">References (2-3) **
 * <span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;">Q: **<span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;"> Why do we use lysozyme? Why do we use Benzonase/Cyanase?
 * <span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;">Q: **<span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;"> Explain what is in the sample after each step: Sample 1, 2, 3,4 ,5, and 6
 * <span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;">Q: **<span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;"> What is different about the Wash vs. the Elution buffer?
 * <span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;">Q: **<span style="font-family: 'Arial','sans-serif'; font-size: 14.6667px;"> Briefly, explain how the HIS tag system works