ANNA+B.

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__ Introduction: __

__ Materials & Methods: __

__ Results: __



Figure 1a: Bacterial growth with DNA plasmid added. Plate with ampicillin. [02-27-14, SOC media, BL21 (DE3) bacteria, pGEM, gbr22 DNA plasmid]

Figure 1b: Bacterial growth with no DNA plasmid added. [02-27-14, SOC media, BL21 (DE3) bacteria, pGEM, no DNA]

Figure 1c: Fun plate with bacteria from a sink sponge. No ampicillin.

Figure 1d: Another group’s bacteria with DNA. This the sample used for growing the starter culture. [02-27-14, BL21(DE3) SOC media, pGEM]

Figure 2a: Sample 1 after letting the new bacteria cells recover and grow. [02-28-14, BL21(DE3), pGEM, gbr22 DNA, ampicillin, LB broth] Figure 2b: Sample 2 after letting the new bacteria cells recover and grow. [02-28-14, BL21(DE3), pGEM, gbr22 DNA, ampicillin, LB broth] Figure 3a: Sample #1 after centrifuging and disposing of the waste; new bacterial cells present in purple. Figure 3b: Sample 2 after centrifuging and disposing of the waste; new bacterial cells present in purple. Figure 4: Sample after purification. The purple is Elution 1. The white is Elution 2.

Figure 5: Samples 1-6 flow throughs, stained on the gel. Gel is dried and labeled.

__ Discussion: __

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