Glyceraldehyde-3-phosphate+Dehydrogenase+(bacillus+anthracis)

 **Target (protein/gene name):** Gyceraldehyde-3-phosphate Dehydrogenase/ GAPDH [|https://www.cdc.gov/anthrax/index.html] [|https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1769905/#__sec1title]
 * E.C Number:** 1.2.1.12
 * NCBI Gene # or RefSeq#: **AIF58743.1 []
 * PDB ID: **4DIB
 * Organism:** Bacillus Anthracis
 * Disease Information: **Bacillus anthracis is a large, gram positive bacterium that causes anthrax, most commonly in animals and occasionally in humans. Bacillus anthracis is the only pathogen within its genus, bacillus. Anthrax is transmitted from inhaling or ingesting spores in contaminated soil, plants, or water. People get infected with anthrax when the spores get into the body and activate. Once the spores become active, they multiply inside the body and release toxins. To prevent an outbreak in areas with animals that have anthrax, routine vaccines are performed. Although anthrax is very rare in the US, it has been used as a biological weapon in 2001, killing 5 of the 22 that were infected. Bacillus anthracis has a high chance of being used as a biological weapon because of how easy the spores can be found in nature and the spores can be put into foods and water without anyone knowing.

[|http://www.ebi.ac.uk/interpro/potm/2004_2/Page3.htm]
 * Essentiality:** Glyceraldehyde-3-phosphate dehydrogenase serves as an important enzyme for very diverse functions. GAPDH catalyzes the sixth step of glycolysis, breaking down glucose for energy and carbon molecules. Depending on the location of GAPDH, it has non-glycotic functions. The translocation to the nucleus acts as a signaling mechanism for apoptosis.

**Druggable Target:** Glyceraldehyde-3-phosphate Dehydrogenase in a different organism, Trypanosoma cruzi, has a druggability of 0.3. []
 * E.C. # ** 1.2.1.12

[|https://www.biovision.com/documentation/datasheets/K680.pdf] Cost of 3-phospho-D-phosphate: $281.00 Cost of NADH: $130.00 Cost of GAPDH: $61.00
 * Enzyme Assay:**
 * from Sigma-Aldrich**

Structure: **4DIB from PDB**
 * []**

Current Inhibitors: **Known inhibitors 4: R-Deprenyl. HCl, Heptelidic Acid, Deprenyl, CGP 3466 maleate**

Expression Information: **Has been expressed in E. coli BL21(DE3).**

Purification Information: **Ammonium sulfate fractionation and one chromatographic step- dye affinity chromatography on Blue Sepharose.**
 * []**

PyMol image of Protein: Fig 1. PyMol structure of Glyceraldehyde-3-phophate dehydrogenase obtained from the PDB. Structure shown in sticks.

Amino Acid Sequence: Length of sequence: **342 amino acids**
 * AIF58743.1 glyceraldehyde-3-phosphate dehydrogenase [Bacillus anthracis] **
 * MTRVAINGFGRIGRMVFRQAIKESAFEIVAINASYPSETLAHLIKYDTVHGKFDGTVEAFEDHLLVDGKM **
 * IRLLNNRDPKELPWTDLGVEVVIEATGKFNAKEKAILHVEAGAKKVILTAPGKNEDVTIVVGVNEDQLDI **
 * TKHTVISNASCTTNCLAPVVKVLDEQFGIENGLMTTVHAYTNDQKNIDNPHKDLRRARACGQSIIPTTTG **
 * AAKALAKVLPHLNGKLHGMALRVPTPNVSLVDLVVDVKRDVTVEAINDAFKTVANGALKGIVEFSEEPLV **
 * SIDFNTNTHSAIIDGLSTMVMGDRKVKVLAWYDNEWGYSRRVVDLVTLVVDELAKQENVQHI **

Molecular Weight using ExPASy: **37.49206 kDa.**

Molar Extinction Coefficient at 280 nm: **24075**

TMpred graph Image from **[]:**

Blast: Query Coverage:** 100% ** Max % Identities:** 100% ** % Positives:** 100% ** Chain used for Homology:** A **

CDS Gene Sequence (codon optimized): ** ATGACCCGCGTGGCGATTAACGGCTTTGGCCGCATTGGCCGCATGGTGTTTCGCCAGGCG ATTAAAGAAAGCGCGTTTGAAATTGTGGCGATTAACGCGAGCTATCCGAGCGAAACCCTG GCGCATCTGATTAAATATGATACCGTGCATGGCAAATTTGATGGCACCGTGGAAGCGTTT GAAGATCATCTGCTGGTGGATGGCAAAATGATTCGCCTGCTGAACAACCGCGATCCGAAA GAACTGCCGTGGACCGATCTGGGCGTGGAAGTGGTGATTGAAGCGACCGGCAAATTTAAC GCGAAAGAAAAAGCGATTCTGCATGTGGAAGCGGGCGCGAAAAAAGTGATTCTGACCGCG CCGGGCAAAAACGAAGATGTGACCATTGTGGTGGGCGTGAACGAAGATCAGCTGGATATT ACCAAACATACCGTGATTAGCAACGCGAGCTGCACCACCAACTGCCTGGCGCCGGTGGTG AAAGTGCTGGATGAACAGTTTGGCATTGAAAACGGCCTGATGACCACCGTGCATGCGTAT ACCAACGATCAGAAAAACATTGATAACCCGCATAAAGATCTGCGCCGCGCGCGCGCGTGC GGCCAGAGCATTATTCCGACCACCACCGGCGCGGCGAAAGCGCTGGCGAAAGTGCTGCCG CATCTGAACGGCAAACTGCATGGCATGGCGCTGCGCGTGCCGACCCCGAACGTGAGCCTG GTGGATCTGGTGGTGGATGTGAAACGCGATGTGACCGTGGAAGCGATTAACGATGCGTTT AAAACCGTGGCGAACGGCGCGCTGAAAGGCATTGTGGAATTTAGCGAAGAACCGCTGGTG AGCATTGATTTTAACACCAACACCCATAGCGCGATTATTGATGGCCTGAGCACCATGGTG ATGGGCGATCGCAAAGTGAAAGTGCTGGCGTGGTATGATAACGAATGGGGCTATAGCCGC CGCGTGGTGGATCTGGTGACCCTGGTGGTGGATGAACTGGCGAAACAGGAAAACGTGCAG CATATT