Target+-+UDP-N-acetylbacillosamine+N-acetyltransferase+(Campylobacter+jejuni)

http://www.absa.org/riskgroups/bacteriasearch.php?genus=Campylobacter&species=jejuni
 * *Target (protein/gene name): ** UDP-N-acetylbacillosamine N-acetyltransferase
 * *NCBI Gene # or RefSeq#: ** 194709137 (Chain A), 194709138 (Chain B), 194709139 (Chain C), 123141428 (Full)
 * *Protein ID (NP or XP #) or Wolbachia#: **// N/A //
 * *Organism (including strain): ** Campylobacter jejuni
 * Etiologic Risk Group (see link below): ** 2

Campylobacteriosis is a bowel disease caused by the bacteria //Campylobacter jejuni//. This disease is also known as campylobacter enteritis and gastroenteritis. //C.// //jejuni// is most commonly found in animal feces, and it infects the humans through accidental consumption of unclean food. This type of bacteria requires reduced levels of oxygen (3-5% oxygen and 2-10% carbon dioxide levels) in order to grow. In animals, //C.// //jejuni// is known to cause abortion and enteritis, specifically in sheep and cattle. Humans develop food poisoning, diarrhea, enteritis, and a case as severe as Crohn's disease in rare instances. //Campylobacter jejuni// is the leading bacteria that causes diarrheal illness in the United States.
 * *Background/ Disease Information (sort of like the Intro to your Mini Research Write up): **


 * Link to TDR Targets page (if present): ** N/A

Chain A: http://www.ncbi.nlm.nih.gov/protein/3BSY_A Chain B: http://www.ncbi.nlm.nih.gov/protein/3BSY_B Chain C: http://www.ncbi.nlm.nih.gov/protein/3BSY_C Full: http://www.ncbi.nlm.nih.gov/protein/Q0P9D1.1
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.): **

"This type of structure is found in many enzymes that employ CoA-linked donors, including a sugar O-acetyltransferase fromEscherichia coli, GAT, which modifies some galactose species to prevent them triggering the lac operon." http://www.ncbi.nlm.nih.gov/pubmed/18198901
 * Essentiality of this protein: **

"We show that Cj1123c has acetyl-CoA-dependent N-acetyltransferase activity not only on the UDP-4-amino-4,6-dideoxy-GlcNAc intermediate of the N-glycosylation pathway but also on the UDP-4-amino-4,6-dideoxy-AltNAc intermediate of the O-glycosylation pathway, implying functional redundancy between both pathways. We further demonstrate that, despite its somewhat relaxed substrate specificity for N-acetylation, Cj1123c cannot acetylate aminoglycosides, indicating a preference for sugar-nucleotide substrates." http://www.ncbi.nlm.nih.gov/pubmed/19448740


 * Complex of proteins?: **3 Chains.
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **// N/A //


 * *EC#: ** 2.3.1.203


 * Link to BRENDA EC# page: ** http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.203



** Figure 1:   **   Reaction schematic of UDP-N-acetylbacillosamine N-acetyltransferase with acetyl CoA from BRENDA.

 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **// N/A //
 * -- link to Sigma (or other company ) page for assay (see Sigma links below) **
 * -- -or link (or citation) to paper that contains assay information **
 * -- links to assay reagents (substrates) pages. **
 * --- List cost and quantity of substrate reagents, supplier, and catalog # **

PDB #: 3BSY
 * Structure Available (PDB or Homology model) **


 * Current Inhibitors: ** __NONE__

It's been expressed in //Escherichia coli.//
 * Expression Information (has it been expressed in bacterial cells): **
 * Figure 2:** Screenshot of expression information for 3BSY in BRENDA.


 * Purification Method : **

"The UDP-N,N‘-diacetylbacillosamine product was purified from the reaction by reverse phase C18 HPLC and the structure determined by NMR analysis. Additionally, the full-length PglF was overexpressed and purified in the presence of detergent as a GST fusion protein, allowing for derivation of kinetic parameters (Olivier)."

http://pubs.acs.org.ezproxy.lib.utexas.edu/doi/full/10.1021/bi061456h


 * Image of protein (PyMol with features delineated and shown separately): **


 * Figure 3:** Cartoon image of protein 3BSY with the 3 chains shown in 3 different colors.


 * Figure 4:** The protein 3BSY shown in sticks. The 3 chains are colored by element with carbon in 3 different colors: carbon in green, blue, then pink. The red represents oxygen, while the blue is for nitrogen.

MARTEKIYIYGASGHGLVCEDVAKNMGYKECIFLDDFKGMKFESTLPKYDFFIAIGNNEIRKKIYQKISE NGFKIVNLIHKSALISPSAIVEENAGILIMPYVVINAKAKIEKGVILNTSSVIEHECVIGEFSHVSVGAK  CAGNVKIGKNCFLGINSCVLPNLSLADDSILGGGATLVKNQDEKGVFVGVPAKRM
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **


 * *length of your protein in Amino Acids: ** 198 amino acids (for 1 chain)


 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website: ** 21.147 kDA


 * Molar Extinction coefficient of your protein at 280 nm wavelength: **



**Figure 5:** Screenshot of the Extinction coefficients of 3BSY from Expasy ProtParam.


 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.


 * Figure 6: ** TMPred output graph for //C. jejuni// protein 3BSY.

atggcgcgcaccgaaaaaatttatatttatggcgcgagcggccatggcctggtgtgcgaa gatgtggcgaaaaacatgggctataaagaatgcatttttctggatgattttaaaggcatg aaatttgaaagcaccctgccgaaatatgatttttttattgcgattggcaacaacgaaatt cgcaaaaaaatttatcagaaaattagcgaaaacggctttaaaattgtgaacctgattcat aaaagcgcgctgattagcccgagcgcgattgtggaagaaaacgcgggcattctgattatg ccgtatgtggtgattaacgcgaaagcgaaaattgaaaaaggcgtgattctgaacaccagc agcgtgattgaacatgaatgcgtgattggcgaatttagccatgtgagcgtgggcgcgaaa tgcgcgggcaacgtgaaaattggcaaaaactgctttctgggcattaacagctgcgtgctg ccgaacctgagcctggcggatgatagcattctgggcggcggcgcgaccctggtgaaaaac caggatgaaaaaggcgtgtttgtgggcgtgccggcgaaacgcatg
 * *CDS Gene Sequence (paste as text only): **


 * *GC% Content for gene: ** 30.83%

http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=192222


 * CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):** //Can't be found.//
 * GC% Content for gene (codon optimized):** //Can't be found.//

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences): **