Target-+Protein+Phosphatase+1+(T.+brucei)


 * Target (protein/gene name): ** Protein Phosphatase 1 (PP1)
 * NCBI Gene # or RefSeq#: **3657166

[|Tb927.4.5030]

Trypanosoma brucei is a protozoan with flagella that causes African Sleeping Sickness (African trypanosomiasis) in humans. The disease is endemic in regions of sub-Saharn Africa. Symptoms occur in two stages. The first stage is characterized by fever, headaches, joint pains, and itching. Invasion of the circulatory and lymphatic systems by the parasites can result in swelling of the lymph nodes. The second stage, or neurological phase, is caused by the invasion of the central nervous system by the parasite. This results in disruption of the sleeping cycle. Infected individuals experience a disorganized and fragmented sleep-wake cycle. The tsetse fly serves as both host and vector for trypanosoma parasites. - Now 0.3 Druggability (082413 - Dr .B)
 * Protein ID (NP or XP #) or Wolbachia#: ** XP # 844733.1
 * Organism (including strain): ** Trypanosoma brucei brucei
 * Etiologic Risk Group: ** Risk Group 2
 * Background/Disease Information: **
 * Essentiality of this protein: ** Essential, significant loss of fitness in blood stream forms has been determined. Used for cytokinesis
 * Complex of proteins?: ** Forms complex with regulatory sub-units GM and GL.
 * Druggable Target: ** 0.6


 * EC#: ** 3.1.3.16
 * Link to BRENDA EC# page: **
 * -- ** http://www.brenda-enzymes.info/literature/lit.php4?e=3.1.3.16&r=655670
 * Enzyme Assay information: **
 * -- ** []
 * -- ** []
 * -- List cost and quantity of substrate reagents and supplier: ** pNPP, $79.99 for 100 mL

-- Closest PDB entry using homology model: 2O8A -- Homology Model option: Query Coverage: 303 Max % Identities: 76% % Positives: 89% Chain used for homology: Chain A
 * Structure Available (PDB or Homology model) **


 * Current Inhibitors: ** Calyculinamide A, Calyculin A, C1/C34-Calyculin A, Des-N-methylcalyculin A, Calyculin A 21-acetate, Hemicalyculin A, Calyculin C, Calyculin F, Calyculin J, Calyculin B, Calyculin E, Dephosphonocalyculin A, Calyculin A 11,13,21-triacetate, 11, 13-O-isopropylidene-calyculin A, C9/C35 calyculin

[]
 * Expression Information: ** Has been expressed in E. coli
 * Purification Method: **Western and Northern Blot analysis


 * Image of protein (PyMol with features delineated and shown separately): **

MSVDVDAIIDKLLEVRLSKPGKQVSLSENDVKNLVMRSREILLSQPALLELEAPIKICGD IHGQYYDLIRLFDNGGFPPSANYLFLGDYVDRGKQGLETICLVLAFKVKFPENFFILRGN HECASINRIYGFFDECKRRYNIRLWKVFTDTFNCLPVACIIDDKIFCCHGGLSPDLQSME QIKKIERPCDVADTGLICDLLWSDPEEGLSGWGENDRGVSYTFGQDIVAKFLSRHDFDLI VRAHQVVEDGYQFFATRQLITIFSAPNYCNEFDNSGAVMSVDADLLCSFQILKPSVKKPK YFQ
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **
 * length of your protein in Amino Acids: ** 303 amino acids
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website: ** 34443.5 kDa
 * Molar Extinction coefficient of your protein at 280 nm wavelength: ** 32150
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.

ATGTCACAGGAGGGTGGGAAATCAGAACCGGACGCCAACGAAAAAGAGCAACGGAACCAAAAATTAAGGC TAGGCCTTGAGGCCATGTTCTGTGCTCAAGAGATACCTTCTGAGAAACGCACGGACATTGAAAGTGACGG CGAGGAAGTGCCTCCAAGCATTACTCGAGATGAAGATAGATACCGTCAGGTGGCGGAGGCTATTCTTAGG ATTGATACGAAGAGCAAAATCATAGAGATAAATAATATTCGCCTGTTTTCAGTGGCTGAAATTGAGCTTG ACAAGTTGACTGAATGCACATCCCTTTCCTTGCGCAAGAATCTCTTGCATGATCTTATTCCGTTTCCTGA GGACCTCGCCGATCGCCTTGATGAGTTGGATCTTTTCGACAACAAAATTCGTAAGCTGAACGATTTTTTC GAGACAGTAACAGTCCCTGGTGACCCGCCCACGACGAAAACACTCCCCAACGCATACAAATGTCTCACGA AATTGGACCTAAGTTACAATCAGATTCGTGAAATCGGTGGACTCGATTCCATCGGGGGTACGCTGAGAGA GCTTTATCTGGTCGAAAATAAAATTAAAGAGGTTAAAAATCTTGATTCTCTGGTGAACCTTGAGTTACTT GAATTAGGGGGCAACCGTTTACGTGCCATCGGTTCCGGGTTGGAGAAGTTGACGAAGTTGAAACAGTTGT GGCTCGGCAAGAATAAGATAAGTTCCATTGGTACCGCGCTTCACAAATTGGTTTCGCTAGAGATCCTTAG TTTGCAGGCAAACCGAATTACCTCTGTTGATGCGGAAAACTTCTTGGGAGCAAAGGCCAATCCTAATTTG AGGGAAGTATACTTGTCCGAGAACGGCCTTACTTCTGTTGGGAACGTGCGACACCTATCAACAATCAAAA TTATCGATTTTAGTTTCAACTCGATATGTTCAATCGACGCTGAAGAGATCAATCCGCAAACCATGCCCAA ACTTGAGGAGTTTTGGCTCACTGATGGAAATATAGCAGATTGGGAAGAGGTAGGAAAGTTAAGTGGCTTC ACAAGCACTTTGAAGACCGTGTATCTTGAGCGTAACCCAATTGAAGAAGATAAGAGGTACCGCGACAAGG TATACATGTACCTGCCCTTCTTGGTACAGATTGATTCATGGCCAATTGTGAATAAGGGAAATCTTGAAGC TGATCGTAAGCGAAAAGCTTGA
 * CDS Gene Sequence (paste as text only): **


 * GC% Content for gene: ** 44.6%

-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **
 * *GC% Content for gene (codon optimized): **
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences): **