phosphatidylinositol+4-kinase+(+plasmodium+vivax)


 * *Target (protein/gene name **** phosphatidylinositol 4-kinase, putative ** (// Plasmodium vivax //
 * *NCBI Gene # or RefSeq#: ** [|126793] / [|XM_001613220.1.]
 * *Protein ID (NP or XP #) or Wolbachia#: **** XP_001613270.1 **
 * *Protein ID (NP or XP #) or Wolbachia#: **** XP_001613270.1 **

***Organism (including strain):** // Plasmodium vivax // Plasmodium vivax is both a pathogenic protozoal parasite. It one of the five species is responsible for human malaria. Although not as deadly as Plasmodium falciparum. It can cause sever disease and death via splenomegaly. They are most commonly found in female Anopheles mosquito and only be transmitted by female because only female mosquito bites. Plasmodium vivax originated in the United States, Latin America, and small part of Africa; however, it has now an emerging problem for developing centuries. Once red blood cells of hosts are infected by Plasmodium vivax, they rupture, leading to fever. Ruptured red blood cell also have tendency to stick to endothelia of blood vessels. When vessels are clogged, RBC deliver oxygen to tissues efficiently causing oxygen deprivation of the tissue. Some other symptoms may also include enlargement of the spleen. Additionally, the protozoal parasite can go dormant for days to years. The primary treatment for Plasmodium vivax is chloroquine.
 * Etiologic Risk Group (see link below): **
 * */Disease Information (sort of like the Intro to your Mini __Research Write__ up): **

**Link to TDR Targets page (if present): N/A**

**Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.)**

[]
 * Essentiality of this protein: **

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3940870/ - The Plasmodium vivax phosphatidylinositol 4-kinase (PI4K) is an essential protein required for the life stage of PI4KIIIβ. PI4KIIIβ is a ubiquitous eukaryotic enzyme that phosphorylates lipids to regulate intracellular signaling and trafficking, hence often a target for drug discovery. To be more specific, Type III phosphatidylinositol 4-kinase (PI4KIIIbeta) is an essential enzyme for organizing membrane trafficking, which is required for a variety of pathogenic processes.

Is it a monomer or multimer as biological unit**? (make prediction at**@http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): Monomer **Complex of proteins?:**

http://pubs.acs.org/doi/ipdf/10.1021/acs.jmedchem.5b01311
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **

***EC#:** 2.7.1.67
 * Link to BRENDA EC# page: **

http://www.brenda-enzymes.org/enzyme.php?ecno=2.7.1.67

**--** Show screenshot of BRENDA enzyme mechanism schematic



**Figure 1.** BRENDA enzyme mechanism schematic of the 1-phosphatidylinositol 4 kinase ( 2.7.1.67).

**Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**

http://www.jbc.org/content/277/22/20041.full

**-- link to Sigma (or other company) page for assay (see Sigma links below)**

**-- -or link (or citation) to paper that contains assay information**

**-- links to assay reagents (substrates) pages.**

**--- List cost and quantity of substrate reagents, supplier, and catalog #**

1 g ATP (Sigma, **// A2383 //** ) - $52.50

**Structure (PDB or Homology model)**

-- For Homology Model option:

Show pairwise alignment of your BLASTP search in NCBI against the PDB

Query Coverage:23%

Max % Identities:44%

% Positives 67%

Chain used for homology: Chain A, Phosphatidylinositol 4-kinase Iii Beta-pik93 In A Complex With Rab11a-gtp Gammas

**Current Inhibitors: i** midazopyrazines

**Expression Information (has it been expressed in bacterial cells):** Has been expressed in E. coli

https://www.ncbi.nlm.nih.gov/pubmed/?term=24876499

**Purification Method** X-RAY DIFFRACTION
 * Image of protein (PyMol with features delineated and shown separately): **



Figure 2. PyMOL cartoon representation of Phosphatidylinositol 4-kinase III beta-PIK93 in a complex with Rab11a- GTP gammaS found in E. Coli; PDB file 4D0L

>EDL43543.1 phosphatidylinositol 4-kinase, putative [Plasmodium vivax] MDHKTDGKEDHKREGKTEGETGQTGKEDAGDTTEGADQREKWTAAPEESTSYRSLYKHNYKAHVYADDFV CLRLKGRGGEGHDDGSSEPQQCVEPFEGDLPVGGCPHDDTPVGELSTKRDNNEEVAATGRDNLCGVAVDR IDEANPPREDAQYVQVGSRMEDHQPDEAIYSQAGTQHSNHANEEKRENSDLQPHSDDNYTLQESYSSDCH LKEEMPHESNQPSEDLPSWEAHKMKEKDQPPEKQNKIKIIEMFKMKLESKEMNRSRHFGDDVRGGGPGGA HHDGYGDGRDDGRDDGRDDGHDDGHGDGHDHSGGASPGASNRCTQIRESISRIFSESPSSKPKIISELKH GEGHPNSGSNGSGSHHSSGTHHGSGSNGSGSHERERADGKANGAKNALKEGSLLRLFRCEYFDTHLHIRY LYDRREVGVHEYLVNSLYTQRKYEDILFYLPQLSQISLVRYESSSLYRFLLCKASNSMHFALKLSWIYHS IVEDNSSKYKDLAHKMTQEIEMAVVNCKPFNGKGTSGNENKQSYLLNLAHPLLFKRKYLIKRIKDNERFE RTKLSRNCLNRSCNLCLLKGGEESTQSGMEVAVQGETQRNSQSADLSSAPNAAPSAAPNAAAHAATHAAA HASPKKRRKREATSTAPPPKLPQCYIVNSGALSIARARVKLPSTYAKLGNPLSASKFFLPEFNCTFDMIG ELQQFFMKQRRCDYFSLLNNFVNLTISVSNLLSTEPDVEIRNELLNRFIYSLNSWMLMRRCIVAACTNVF SMTGLCIPLECLSPPQSGQRTEPTPSSNGSNSNGSSNISGSSGSSGSGLQILHFNYDECKIFFSKKRAPY LLMFEVADLDEDISHIPDGLFYPSSVGGDEAAGEEEEEAGQTASETAGEAADGATRGVTDEPTSQPTPPP RRKGHRIERNFCSSKNYGLFNENSSSYDYEPRGGVHDGKRKKKKKKKKRRSESGEDDEGGAHTGQSGSEV DRGKEERGKGAQGKKTTGADLDALKMEHMYVYNCIVNDLRKENLISFPSGEEDSLSIIRKCIGMRADEED EEGGDTEGGDTNGGDPGDPSEPSNLGQGGPGWGAPPAEEGPREATKSFLVNARSASMPNYLSSSLERCNI SSNDNDSNEVKTSVESISSGLADLPPGGLHGSEVDAQVDAQEDAQVEAPDGGWSPSPGKRPNDSGVTADQ PLEGAAPSPHSSFLHAGEKTGDALDIDEYFKPANYTNEEFKKRYCRVIKRLLWGELFEEKKKKIRKTSAY GKLKTWDLKCVIVKGGDDLRQELLASQLIRQFKIIFENAGLPLWLRPYEILVTGANSGIIEYVNDTCSVD SLKRKFGADSISTIFNVVFADYIFEAKKNFIESHAAYSLVSYLLQVKDRHNGNLLLDSDGHLIHIDYGFM LTNSPGNVNFETSPFKLTQEYLDIMDGEKSENYEYFRRLIVSGFLEARKHSEEIILFVELMMPALKIPCF ANGTQFCIDSLKERFMTNLAVDVCIQRINALIESSINNFRSVQYDYFQRITNGIM
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **

***length of your protein in Amino Acids** 1525 aa


 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website **

168828.92 Da

**Molar Extinction coefficient of your protein at 280 nm wavelength:** 125875 mol-1 cm-1

**TMpred graph Image**( @http://www.ch.embnet.org/software/TMPRED_form.html ). Input your amino acid sequence to it.


 * *CDS Gene Sequence (paste as text only): **

atggaccaca aaacagatgg caaagaagac cacaaaagag aaggcaaaac tgaaggcgaa acgggacaga ccggcaagga agatgctggc gacacaaccg agggcgccga ccaaagggaa aagtggaccg ccgcaccgga agagagcaca tcgtataggt ctctgtacaa acacaactac aaagcgcacg tgtacgcgga tgattttgtg tgccttcgat tgaaaggtcg tggaggtgag gggcacgatg atggaagcag tgaaccgcag cagtgtgtag agccgttcga gggggatctc ccagtgggtg gctgcccaca tgatgataca cctgttgggg agttaagtac taaaagggac aacaatgagg aggttgctgc cacagggagg gacaacctgt gtggggttgc agtcgatagg attgacgaag caaacccccc aagggaggat gctcagtacg ttcaggtagg cagcagaatg gaggaccacc aaccagacga agcgatatac tcccaagcgg ggacacaaca tagcaatcac gctaatgagg agaagaggga aaactcggat cttcagcctc actccgatga taattacacc cttcaagaaa gctacagcag tgactgccat ctgaaggagg aaatgccaca cgagagtaac caacccagtg aggacctgcc cagctgggaa gcacacaaaa tgaaggagaa ggaccaaccc ccagagaaac aaaacaaaat taaaattatt gaaatgttta agatgaagct ggagagtaag gagatgaaca ggagtcgcca tttcggtgat gacgtccgtg gggggggtcc cggtggcgct catcacgatg ggtatggcga tgggcgtgac gatgggcgtg acgatgggcg tgacgatggg catgacgatg ggcatggtga tgggcatgac cactcggggg gcgcctcccc cggtgcctct aaccgctgca cgcagatacg ggagagcatc tctcgcatat tcagcgagtc gccctcctcc aagccgaaga taataagcga gctgaagcac ggcgaggggc accccaacag cggtagcaac ggaagcggca gccaccacag cagcggtacc caccacggaa gcggtagcaa cggaagcggc agccacgaac gggagagggc ggacggaaag gccaacggag cgaagaacgc attgaaggag ggaagcctct tgaggctctt cagatgcgaa tactttgaca cccacctgca cattaggtac ctctacgaca ggagagaagt gggggtccac gagtacctgg tgaattccct ttacacgcag aggaagtacg aggatatatt attttacctc ccgcagctca gtcaaatttc gctagtccgt tacgagtcct cttccctcta tcgcttcctc ctttgcaaag ccagcaactc catgcacttc gcattaaagc tcagctggat ctatcattct atagtagagg acaactcttc aaaatataag gacttggctc acaagatgac tcaagaaatt gaaatggctg tggtgaattg caaaccattt aacggtaaag gcacctcggg gaacgagaat aagcagtcgt acctgctgaa cctcgcccac ccgctcctct tcaaaaggaa gtacctcatt aaaaggatca aagataatga gcggttcgag cgcacgaagc tatccaggaa ttgcctgaac aggtcatgca acttgtgctt gctgaagggg ggggaagagt ccacgcagag cgggatggaa gtggctgtgc aaggcgaaac gcagaggaac tcccaaagcg ctgatctgag ttccgctcca aatgccgctc caagcgccgc tccaaacgcc gctgctcatg ccgccacaca tgccgctgca catgcctcgc ccaagaagcg gcgcaaacgg gaggcgacga gcacagcgcc gccccccaag ctgccccagt gctacattgt caactcgggc gccctctcca tagccagggc cagagtgaag ctccccagca cctacgccaa gctgggcaac cccctcagcg catccaagtt cttcctccca gaatttaatt gcacctttga tatgatagga gagctgcagc agttttttat gaagcagagg aggtgcgatt actttagcct cctgaacaac tttgtgaact tgaccatctc cgtttctaac cttctgtcca ccgaaccaga tgtagagata cgcaacgagc tcctcaacag attcatttac tctttaaaca gttggatgct catgcgtagg tgcatcgtgg ctgcatgtac taacgttttt tccatgacgg ggctgtgcat tccgctcgag tgcctgtccc ccccccagag cggccagcga acggagccga ccccaagcag caatggcagc aatagcaacg gtagcagcaa catcagcggt agcagcggca gcagcggcag cggcttgcag atccttcact ttaactacga tgagtgcaaa atatttttct ccaagaagag ggcgccctac ctgctcatgt tcgaggtggc ggacctggac gaggacatca gccacattcc ggacggtctc ttttacccct ccagcgtggg gggggatgaa gcggcgggtg aggaggagga agaagcaggt caaacggcga gcgaaacggc gggtgaggca gcggatggtg caacccgtgg cgtgactgac gaaccgacgt cgcaaccgac gccgccaccg cgaaggaagg gccaccgcat cgaaaggaac ttctgttcct ctaaaaacta cgggctcttc aacgagaaca gcagctccta cgactacgag ccgcgaggcg gggtgcatga tgggaagagg aagaagaaga agaaaaagaa gaagaggaga agcgaaagtg gggaggacga cgaggggggg gcgcacaccg gccagagtgg ttcagaagtg gatcggggga aggaggaacg cgggaaaggc gcgcagggaa aaaaaacaac cggcgcggac ctggacgctc tgaaaatgga gcacatgtac gtatacaact gcatagtgaa cgacctgcga aaggagaatt tgatttcgtt cccctcgggg gaggaggaca gcctctccat aattaggaag tgcataggga tgagggcgga cgaggaggac gaggaggggg gagacacgga ggggggcgac acgaacgggg gcgatccggg cgatccgagc gaaccgagca acctcgggca gggcggcccc ggttgggggg ccccccccgc ggaggaaggc ccccgggagg caaccaagag cttcctggtg aacgcccgct ccgcgtctat gcccaactat ctaagtagct ccctggagag gtgcaacatc agctcgaacg acaatgacag caatgaagtg aaaacgtctg tggagagcat cagcagtggg ctggctgacc tcccacctgg ggggttgcac ggaagtgagg tggacgctca ggtggacgcc caggaggatg cccaggtgga agctccagat ggaggatgga gcccctcccc gggtaaacgc cccaatgata gtggtgtcac tgcagatcaa ccgttagaag gcgccgcccc ctccccacac agcagcttct tacacgcggg ggagaaaaca ggcgacgcgc tagacatcga cgagtacttc aaaccagcga attacaccaa cgaggaattt aaaaaaaggt attgtagagt tataaagagg ctgctatggg gagagctatt tgaagaaaaa aaaaaaaaaa ttaggaaaac gtcagcttat gggaagttaa agacatggga tttgaagtgc gtaatcgtta agggtgggga tgacttgagg caggagctgc tggcatccca actgattcgc cagtttaaaa ttatttttga gaatgcgggc ctgccgctgt ggctacgccc ctacgaaatt ctagtgacgg gggcaaactc cggcatcatt gaatacgtaa atgacacctg ctcggtggac tcgctcaagc ggaagttcgg cgccgacagc atctccacca tcttcaacgt ggtcttcgcg gactacatct tcgaggccaa gaagaacttc atcgagagcc acgcagccta ctcgctcgtc tcgtacctcc tccaggtgaa ggacaggcac aacggaaacc tcctgctgga ctcggacgga cacctcatcc acatagacta cggcttcatg ctcaccaact cccctggaaa cgtaaatttt gagacctccc ccttcaaact cacccaagaa tacctagaca tcatggacgg agaaaagtca gaaaattatg agtacttcag gcgcctcatc gtcagtggct tcctcgaggc ccgcaagcac tcggaggaga tcatcctctt cgtcgagctg atgatgccag ctttaaaaat cccctgcttt gcgaacggca cgcagttctg catcgactcg ctgaaggagc gcttcatgac gaacctggcc gtcgacgtgt gcatccaacg aatcaacgcc ctcattgagt cctccatcaa taattttaga agcgtccagt acgactactt ccagagaatc accaacggaa ttatgtga ***GC% Content for gene:** 54.9%

***CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):**

***GC% Content for gene (codon optimized):**