TargetSp17-+Ser_Thr+Protein+Kinase+B+PKNB+(Mycobacterium+Canettii)


 * *Target (protein/gene name): ** Serine/Threonine- Protein Kinase B PkNB
 * *RefSeq#: ** NC 015848.1, WP 003400356.1
 * *Protein ID (NP or XP #) or Wolbachia#: **G0TLP7-1
 * *Organism (including strain): **//Mycobacterium canettii (strain CIPT 140010059) ( //complex// of //Mycobacterium tuberculosis)
 * Etiologic Risk Group (see link below): ** RG2

Mycobacterium tuberculosis is an obligate pathogenic bacterial species that causes tuberculosis. Humans are the only known reservoirs of M. tuberculosis and can be spread through air droplets originating from a person who has the disease either coughing, sneezing, speaking, or singing. Tuberculosis is one of the oldest human diseases and remains a major cause of death in some countries. Tuberculosis can affect bone, central nervous system, and other vital organ systems but is a pulmonary disease caused by Mycobacterium tuberculosis transmitted through the air. New strains of Mycobacterium tuberculosis appear often that are more virulent and require new vaccines and drugs. Mycobacterium Canetti is very similar to Mycobacterium tuberculosis and belongs to both belong to the Mycobacterium tuberculosis complex(MTBC) which is a group of genetically similar Mycobacterium species that can cause tuberculosis in living things.
 * Disease Information**


 * Link to TDR Targets page (if present): **[]
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.): UniProt: [] **

The Ser/Thr protein Kinase (STPK) is essential for the Mycobacterium Canettii to survive because it plays an important role in regulating growth and division. STPK mediates protein phosphorylation which is essential for cells to transduce extracellular signals into responses. Ser/Thr kinase activity has numerous effects on the cells that can affect transcription of DNA and immune defense. Without the precise functioning of the kinase the accurate signal to control the growth of cells will not be transduced in the signal pathway.
 * Essentiality of this protein: **

On Druggability Index (range 0-1): 0.7 [|The structure of PknB in complex with mitoxantrone, an ATP-competitive inhibitor, suggests a mode of protein kinase regulation in mycobacteria]
 * Is it a monomer or multimer as biological unit? (make prediction at ** @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): The structure appears to be monomeric
 * Complex of proteins: ** Functions with a Protein Kinase Domain and works with protein kinases
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **


 * *EC#: **2.7.11.1
 * Link to BRENDA EC# page: **[]



// Figure 1. Reaction Mechanism produced by the ser/thr protein Kinase from BRENDA. // Array-Based Fluorescence Assay
 * Enzyme Assay information : **
 * -- to paper that contains assay information: **[]
 * -- links to assay reagents (substrates) pages. **
 * --- Lists cost and quantity of substrate reagents, supplier, and catalog # **

-- **[|PDB #] :** 1MRU **Similarity to Humans:** After running BLASTP with the amino acid sequence of STPK there was a match of 81% to human STPKs. Inhibiting the inducible dimerization system has been looked [|into in this paper.]
 * Structure (PDB or Homology model) **
 * Current Inhibitors: **


 * Expression Information : ** The protein can and has been expressed in E.Coli bacteria. [|Paper Describing expression of the protein].
 * Purification Method:** Purified by washing in a solution of 10% B-Per reagent and centrifuging. Protein can be separated with sodium dodecyl sulfate. [|Purification Process].

// Figure 2. Protein structure of 1MUR from PDB. // Chain A Sequence: GSHMTTPSHLSDRYELGEILGFGGMSEVHLARDLRLHRDVAVKVLRADLARDPSFYLRFRREAQNAAALNHPAIVAVYDT
 * Image of protein (PyMol with features delineated and shown separately): **
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **

GEAETPAGPLPYIVMEYVDGVTLRDIVHTEGPMTPKRAIEVIADACQALNFSHQNGIIHRDVKPANIMISATNAVKVMDF

GIARAIADSGNSVTQTAAVIGTAQYLSPEQARGDSVDARSDVYSLGCVLYEVLTGEPPFTGDSPVSVAYQHVREDPIPPS

ARHEGLSADLDAVVLKALAKNPENRYQTAAEMRADLVRVHNGEPPEAPKVLTDAERTSLLSSAAGNLSGPR

Chain B Sequence:

GSHMTTPSHLSDRYELGEILGFGGMSEVHLARDLRLHRDVAVKVLRADLARDPSFYLRFRREAQNAAALNHPAIVAVYDT

GEAETPAGPLPYIVMEYVDGVTLRDIVHTEGPMTPKRAIEVIADACQALNFSHQNGIIHRDVKPANIMISATNAVKVMDF

GIARAIADSGNSVTQTAAVIGTAQYLSPEQARGDSVDARSDVYSLGCVLYEVLTGEPPFTGDSPVSVAYQHVREDPIPPS

ARHEGLSADLDAVVLKALAKNPENRYQTAAEMRADLVRVHNGEPPEAPKVLTDAERTSLLSSAAGNLSGPR

// Figure 3. TMpred Graph of the STPK protein. // ATGAGCGACGTGGCTATTGTGAAGGAGGGTTGGCTGCACAAACGAG GGGAGTACATCAAGACCTGGCGGC CACGCTACTTCCTCCTCAAGAATGATGGCACCTTCATTGGCTACAAGGAGCGGCCGCAGGATGTGGACCA ACGTGAGGCTCCCCTCAACAACTTCTCTGTGGCGC AGTGCCAGCTGATGAAGACGGAGCGGCCCCGGCCC AACACCTTCATCATCCGCTGCCTGCAGTGGACCACTGTCATCGAACGCACCTTCCATGTGGAGACTCCTG AGGAGCG GGAGGAGTGGACAACCGCCATCCAGACTGTGGCTGACGGCCTCAAGAAGCAGGAGGAGGAGGA GATGGACTTCCGGTCGGGCTCACCCAGTGACAACTCAGGGGCTGAAGAGATGGAGGTGTCCCTGGCCAAG CCCAAGCACCGCGTG ACCATGAACGAGTTTGAGTACCTGAAGCTGCTGGGCAAGGGCACTTTCGGCAAGG TGATCCTGGTGAAGGAGAAGGCCACAGGCCGCTACTACGCCATGAAGATCCTCAAGAAGGAAGTCATCGT GGCCAAG GACGAGGTGGCCCACACACTCACCGAGAACCGCGTCCTGCAGAACTCCAGGCACCCCTTCCTC ACA GCCCTGAAGTACTCTTTCCAGACCCACGACCGCCTCTGCTTTGTCATGGAGTACGCCAACGGGGGCG AG CTGTTCTTCCACCTGTCCCGGGAGCGTGTGTTCTCCGAGGACCGGGCCCGCTTCTATGGCGCTGAGAT TGTGTCAGCCCTGGACTACCTGCACTCGGAGAAGAACGTGGTGTACCGGGACCTCAAG CTGGAGAACCTC ATGCTGGACAAGGACGGGCACATTAAGATCACAGACTTCGGGCTGTGCAAGGAGGGGATCAAGGACGGTG CCACCATGAAGACCTTTTGCGGCACACCTGAGTACCTGGCCCCCGAG GTGCTGGAGGACAATGACTACGG CCGTGCAGTGGACTGGTGGGGGCTGGGCGTGGTCATGTACGAGATGATGTGCGGTCGCCTGCCCTTCTAC AACCAGGACCATGAGAAGCTTTTTGAGCTCATCCTCATGGAGGAGATCCGCTTCCCGCGCACGCTTGGTC CCGAGGCCAAGTCCTTGCTTTCAGGGCTGCTCAAGAAGGACCCCAAGCAGAG GCTTGGCGGGGGCTCCGA GGACGCCAAGGAGATCATGCAGCATCGCTTCTTTGCCGGTATCGTGTGGCAGCACGTGTACGAGAAGAAG CTCAGCCCACCCTTCAAGCCCCAGGTCACGTCGGAGACTGACACCAGGTATTTTGATGAGGAGTTCACGG CCCAGATGATCACCATCACACCACCTGACCAAG ATGACAGCATGGAGTGTGTGGACAGCGAGCGCAGGCC CCACTTCCCCCAGTTCTCCTACTCGGCCAGCGGCACGGCCTGA
 * *length of your protein in Amino Acids: ** 644
 * Molecular Weight:** 69555.03
 * Extinction coefficient:** 30175
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html).
 * CDS ****Gene Sequence:**

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)

**
 * Primer design results for 'tail' primers (this is just 2 sequences): **