ALBERTO+T.

__ Title: __

__ Introduction: __

__ Materials & Methods: __

__ Results: __ [[image:AT_VDS_TransformationFigure 1_030714.jpeg width="391" height="295" caption=" "]] Figure 1. Digital representation of the LB Agar plate with ampicillin, soc media, LB media, organism: BL21 (DE3), and plasmid: pGEM-gbr22. Grown in the 37C incubator overnight. Bacterial colonies are purple dots.

 Figure 2. Digital representation of the LB Agar plate used as control. No DNA present. Antibiotic used: ampicillin. Organism: BL21 (DE3). Left overnight in the 37C incubator. No growth observed.

 Figure 3. Digital representation of Agar plate used as “Fun plate”. No DNA or antibiotic present, just LB media and Agar. Colonies have a yellow like color. Growth after 24 hours in the incubator.

 Figure 4. Digital representation of Agar Plate used as “Fun Plate”. No DNA or antibiotic present, jus LB media and Agar. Colonies have a yellow like color, Growth after 3 days in the incubator.

 Figure 5. Digital representation of the Erlenmeyer flasks containing 25mL of LB media, ampicillin, and bacteria BL21(DE3) after 36 hours in the 37C shaking incubator.

 Figure 6. Resuspended pellet in 1xPBS solution. The pellet weighed 0.51g. Organism: BL21(DE3). Plasmid: pGEM-gbr22.

Figure 7. Digital representation of elution 2 (left) and elution 1 (right) resuspended in 1x PBS and 250mM imidazole in 15ml conical tubes. Elution 1 – 5ml. Elution 2 – 4.5ml.

Figure 8. Absorbance vs wavelength graph for Elution 1 (p-gbr22) at 280nm. Obtained using nanodrop spectrophotometer.

Figure 9. Absorbance vs wavelength graph for Elution 1 (p-gbr22) at 574 (black plot) and at 280 (blue plot). Obtained using nanodrop spectrophotometer.

Figure 10. Polyacrylamide gel for Samples 1 through 6 before drying and after staining. Protein pgbr22.

Figure 11. Dry polyacrylamide gel for Samples 1 through 6. Protein pgbr22.

__ Discussion: __

__ Conclusions: __

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