TAYLOR+C.

__ Title: __

__ Introduction: __

__ Materials & Methods: __

__ Results: __ Figure 1: The bacteria/SOC mixture grown overnight is displayed in purple on the LB Agar Amp Plate. The plasmid pGEM-gbr22 was inserted into the E.coli BL21 host bacteria because it encodes for a fluorescent purple protein and allows the expression to be followed. Figure 2: VDS student Neusha's grown bacterial plate. This bacteria was used to create the solutions to the other parts of the lab. Figure 3: The large protein culture above was created from a smaller protein culture. A starter culture was made using ampicillin, LB, and single colony from the agar plate. The grown starter culture was then mixed in a flask with LB and ampicillin. This large culture was left to grow overnight and the proteins were expressed in purple.

Figure 4: The bacteria from the large culture was placed in 50 ml conical tubes and centrifuged. The purple pellet that was created is composed of cells. The pellet weighs about 0.6 grams. Figure 5:



Figure 6:

Figure 7:

Figure 8:

Figure 9:

Figure 10: 1. Ladder 6. Sample 5 - Elution 1 2. Sample 1 7. Sample 6 - Elution 2 3. Sample 2 8. Sample 5 - Elution 1 4.Sample 3 9. Sample 6- Elution 2 5. Sample 4

__ D ____ iscussion: __

__ Conclusions: __

__ References: __