Target+- NMN+phosphatase,+Class+B+acid+phosphatase+precursor+(Staphylococcus+aureus)

Organism: Staphylococcus aureus

Target: NMN phosphatase; Class B acid phosphatase precursor

EC:3.1.3.2 :Acid phosphatase

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Background and Disease: S. Aureus has a role in causing food poisoning, atopic dermatitis, staphylococcal scalded skin syndrome, or Ritter's disease in neonates.

Enzyme page on Brenda: http://www.brenda-enzymes.org/php/result_flat.php4?ecno=3.1.3.2

Reaction pathway: [|http://www.brenda-enzymes.org/php/result_flat.php4?ecno=3.1.3.2&Suchword=t.+cruzi&organism[=Staphylococcus+aureus&show_tm=0]]

enzyme assay info: []

=** REAGENTS: **=

Sodium hydroxide solution: 199.00

 * Gene #:12865799**

Essentiality of protein: None

Inhibitors: Vancomycin

Image of protein:



Coding DNA Sequence link: http://www.ncbi.nlm.nih.gov/nuccore/JH806568.1

Amino Acid sequence: 1 mraaplllar aaslalascf cffcwldrsv lakelkfvtl vfrhgdrspi dtfptdpike 61 sswpggfggl tglgmeqhye lgeyirkryr kflndsykhe qvyirstdvd rtlmsrmtnl 121 aalfppegvs iwnpillwqp ipvhtvplse dqllylpfrn cprfqelese tlkseefqkr 181 lhpykdfiat lgklsglhgq dlfgiwskvy dplysesvhn ftlpswated tmtklrelse 241 lsllslygih kqkeksrlqg gvlvneilnh mkratqipsy kklimysahd ttvtglqmal 361 gpvipqdwst evmttnshqg tedstd

Primer Design:

 1 ATGCGTGCAGCACCTCTGCTCCTGGCCCGTGCGGCCTCTCTGGCGCTGGCATCTTGC 57  2 TCTTTCGCCAGAACAGAGCGGTCCAGCCAACAGAAGAAGCAGAAGCAAGATGCCAGCGCC 60  3 GCTCTGTTCTGGCGAAAGAACTGAAGTTCGTTACTCTGGTTTTCCGTCACGGTGACCGTT 60  4 GAAGATTCTTTAATCGGATCGGTCGGAAAGGTATCGATCGGAGAACGGTCACCGTGACGG 60  5 GACCGATCCGATTAAAGAATCTTCTTGGCCGCAGGGCTTTGGTCAACTGACGCAGCTGGG 60  6 GCGCTTACGGATGTACTCGCCCAGTTCGTAGTGCTGTTCCATACCCAGCTGCGTCAGTTG 60  7 CGAGTACATCCGTAAGCGCTACCGTAAATTCCTGAACGACTCCTACAAGCATGAACAGGT 60  8 GACATGAGGGTACGGTCAACGTCAGTAGAACGAATGTAAACCTGTTCATGCTTGTAGGAG 60  9 GTTGACCGTACCCTCATGTCTCGTATGACGAACCTGGCGGCACTCTTCCCGCCTGAGGGC 60  10 ACCGGGATCGGCTGCCACAGCAGGATTGGATTCCAGATAGAGACGCCCTCAGGCGGGAAG 60  11 GCAGCCGATCCCGGTTCACACCGTTCCACTCTCTGAAGATCAACTCCTGTACCTGCCGTT 60  12 AGGGTTTCAGATTCGAGTTCCTGGAAACGCGGGCAGTTGCGGAACGGCAGGTACAGGAGT 60  13 GGAACTCGAATCTGAAACCCTGAAATCTGAGGAGTTCCAGAAACGTCTCCACCCGTACAA 60  14 CCGTGCAGGCCAGACAGTTTGCCCAGGGTCGCGATGAAGTCTTTGTACGGGTGGAGACGT 60  15 TGTCTGGCCTGCACGGTCAGGATCTGTTTGGTATCTGGTCTAAAGTTTACGACCCGCTGT 60  16 CCCAAGATGGCAGAGTGAAGTTGTGAACAGACTCGGAGTACAGCGGGTCGTAAACTTTAG 60 <span style="background-color: #ffffff; color: #222222; font-family: arial,sans-serif;"> 17 CTTCACTCTGCCATCTTGGGCGACTGAAGACACCATGACTAAACTGCGTGAGCTGTCTGA 60 <span style="background-color: #ffffff; color: #222222; font-family: arial,sans-serif;"> 18 TCTTTTTGTTTGTGGATACCGTACAGAGACAGGAGGCTCAGTTCAGACAGCTCACGCAGT 60 <span style="background-color: #ffffff; color: #222222; font-family: arial,sans-serif;"> 19 GTACGGTATCCACAAACAAAAAGAAAAATCTCGTCTGCAAGGTGGCGTTCTCGTTAATGA 60 <span style="background-color: #ffffff; color: #222222; font-family: arial,sans-serif;"> 20 GCTCGGGATCTGGGTCGCACGTTTCATGTGGTTGAGAATTTCATTAACGAGAACGCCACC 60 <span style="background-color: #ffffff; color: #222222; font-family: arial,sans-serif;"> 21 CGACCCAGATCCCGAGCTACAAAAAACTGATCATGTATTCTGCGCATGACACGACCGTTA 60 <span style="background-color: #ffffff; color: #222222; font-family: arial,sans-serif;"> 22 GCAGCAGGCCGTTGTAAACGTCCAGGGCCATCTGGAGGCCGGTAACGGTCGTGTCATGCG 60 <span style="background-color: #ffffff; color: #222222; font-family: arial,sans-serif;"> 23 TTACAACGGCCTGCTGCCGCCTTACGCCTCTTGTCACCTCACCGAACTGTACTTCGAAAA 60 <span style="background-color: #ffffff; color: #222222; font-family: arial,sans-serif;"> 24 TCTCGTTGCGATAGTACATTTCAACGAAATATTCACCTTTTTCGAAGTACAGTTCGGTGA 60 <span style="background-color: #ffffff; color: #222222; font-family: arial,sans-serif;"> 25 TGAAATGTACTATCGCAACGAGACCCAGCACGAACCGTATCCGCTGATGCTCCCAGGTTG 60 <span style="background-color: #ffffff; color: #222222; font-family: arial,sans-serif;"> 26 CCAACGAGCTCCGCGAAACGTTCCAGCGGGCAAGACGGAGAGCAACCTGGGAGCATCAGC 60 <span style="background-color: #ffffff; color: #222222; font-family: arial,sans-serif;"> 27 TCGCGGAGCTCGTTGGTCCGGTTATCCCGCAGGACTGGTCCACCGAAGTTATGACCACTA 60 <span style="background-color: #ffffff; color: #222222; font-family: arial,sans-serif;"> 28 GTCGGTAGAGTCCTCGGTGCCCTGGTGAGAGTTAGTGGTCATAACTTCGGTGG 53

<span style="background-color: #ffffff; color: #222222; font-family: arial,sans-serif;">Link to GDocs Output:

https://docs.google.com/file/d/0B4O2KqKh2q_-WER0Q3FuOVl6RkU/edit