TargetSp17-fabH+3-oxoacyl-acyl-carrier-protein+synthase+3+(Francisella+tularensis)

https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?lvl=0&id=83333 Is it a monomer or multimer as biological unit ** ? (make prediction at ** @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): multimer **Druggability index (range: 0 to 1):** 0.3 > [|2 chemical compounds are associated with this gene] > || [|C11H14O2S] || Curated by TDRTargets || [|Show papers] || > || [|C12H16O2S] || Curated by TDRTargets || [|Show papers] || > > || Mycobacterium tuberculosis || [|3-oxoacyl-[acyl-carrier-protein synthase III]] || [|Show papers] || [|show compounds] || > || Haemophilus influenzae || [|Beta-ketoacyl-ACP synthase III] || [|Show papers] || [|show compounds] || > || Streptococcus pyogenes serotype M3 || [|Beta-ketoacyl-ACP synthase III] || [|Show papers] || [|show compounds] || > || Staphylococcus aureus subsp. aureus Mu50 || [|Beta-ketoacyl-ACP synthase III] || [|Show papers] || [|show compounds] || > || Enterococcus faecalis || [|Beta-ketoacyl-ACP synthase III] || [|Show papers] || [|show compounds] || > || Plasmodium falciparum 3D7 || [|Beta-ketoacyl-ACP-synthase III] || [|Show papers] || [|show compounds] || > || Escherichia coli || [|3-oxoacyl-[acyl-carrier-protein synthase 3]] || [|Show papers] || [|show compounds] || > || Plasmodium falciparum || [|3-oxoacyl-(acyl carrier protein) synthase III, putative] || [|show compounds] ||
 * *Target (protein/gene name): ** fabH 3-oxoacyl-[acyl-carrier-protein] synthase 3
 * *NCBI Gene # or RefSeq#: ** 8333
 * *Protein ID (NP or XP #) or Wolbachia#: **[|NP_415609.1.]
 * *Organism (including strain): ** //Escherichia coli (strain K12)//
 * */ Disease Information (sort of like the Intro to your Mini Research Write up): ** //Francisella tularensis// is pathogenic bacterial species that is the causing agent of tularemia, which is lethal without treatment. //F. tularensis// is classified as a Tier 1 select agent by the U.S. government due to its low infectious dose, aerosol dispersion potential, and high virulence.
 * Link to TDR Targets page (if present): **
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) **
 * Essentiality of this protein: **
 * Gene/Ortholog: ** mtu541 (OG4_12762); **Phenotype: ** non-essential; **Source study: ** nmpdr
 * Gene/Ortholog: ** eco1055 (OG4_12762); **Phenotype: ** undefined; **Source study: ** blattner
 * Gene/Ortholog: ** eco1055 (OG4_12762); **<span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;">Phenotype: **<span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;"> essential; **<span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;">Source study: **<span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;"> gerdes
 * <span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;">Gene/Ortholog: **<span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;"> eco1055 (OG4_12762); **<span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;">Phenotype: **<span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;"> non-essential; **<span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;">Source study: **<span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;"> keio
 * <span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;">Gene/Ortholog: **<span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;"> eco1055 (OG4_12762); **<span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;">Phenotype: **<span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;"> non-essential; **<span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;">Source study: **<span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;"> shigen
 * Complex of proteins?: ** No
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **
 * <span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.2px;">**Curated from literature**
 * ~ **Compound** ||~ **Source** ||~ **Reference** ||
 * **Predicted associations**
 * **By orthology to known druggable targets**
 * From ChEMBL**
 * ~ **ChEMBL Species** ||~ **Known druggable target** ||~ **Reference** ||~ **Linked compounds** ||
 * From TDR Targets**
 * ~ **TDR Targets Species** ||~ **Known druggable target** ||~ **Linked compounds** ||
 * **By sequence similarity to non orthologous druggable targets**


 * *EC#: **<span style="background-color: #ffffff; color: #00709b; font-family: Verdana,Arial,sans-serif;">[|2.3.1.180]
 * Link to BRENDA EC# page: **http://www.brenda-enzymes.org/enzyme.php?ecno=2.3.1.180&UniProtAcc=P0A6R0&OrganismID=2026
 * -- ** Show screenshot of BRENDA enzyme mechanism schematic

acetyl-CoA + a malonyl-[acyl-carrier protein] = an acetoacetyl-[acyl-carrier protein] + CoA + CO2


 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **
 * -- -or link (or citation) to paper that contains assay information **

-- PDB # or closest PDB entry if using homology model: - 1EBL What is the PDB identifier - Dr. B
 * Structure (PDB or Homology model) **

<span style="color: #222222; font-family: Verdana,Arial,sans-serif;">Inhibited by the SB418011 antibiotic. Not inhibited by cerulenin and thiolactomycin. immobilized metal ion affinity chromatography (Ni2+)
 * Current Inhibitors: **
 * Expression Information (has it been expressed in bacterial cells): N/A **
 * Purification Method : **

- 719087, 719093 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis

- 736053 recombinant His-tagged enzyme from strain BL21(DE3)

- 665468 recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3)

- 666950 recombinant native and selenomethionine-labeled enzyme from Escherichia coli strain DH10B to homogeneity by two different steps of anion exchange and a step of hydroxyapatite chromatography

- 702661 recombinant selenomethionine-labeled enzyme by ion exchange, hydroxylapatite, and affinity chromatography, and gel filtration

- 663822 to homogeneity in three chromatographic steps (Q-Sepharose, MonoQ, and hydroxyapatite)

- 719085 to homogeneity in three chromatographic steps (Q-Sepharose, MonoQ, and hydroxyapatite), immobilized metal ion affinity chromatography (Ni2+)

- 719068, 719086 code format="sequence" <span style="color: #222222; font-family: monospace,monospace; font-size: 14.04px;">      10         20         30         40         50 MYTKIIGTGS YLPEQVRTNA DLEKMVDTSD EWIVTRTGIR ERHIAAPNET 60        70         80         90        100 VSTMGFEAAT RAIEMAGIEK DQIGLIVVAT TSATHAFPSA ACQIQSMLGI 110       120        130        140        150 KGCPAFDVAA ACAGFTYALS VADQYVKSGA VKYALVVGSD VLARTCDPTD 160       170        180        190        200 RGTIIIFGDG AGAAVLAASE EPGIISTHLH ADGSYGELLT LPNADRVNPE 210       220        230        240        250 NSIHLTMAGN EVFKVAVTEL AHIVDETLAA NNLDRSQLDW LVPHQANLRI 260       270        280        290        300 ISATAKKLGM SMDNVVVTLD RHGNTSAASV PCALDEAVRD GRIKPGQLVL 310 LEAFGGGFTW GSALVRF code
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **
 * *length of your protein in Amino Acids: ** 317
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website: **33515.12
 * Molar Extinction coefficient of your protein at 280 nm wavelength: **25690
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.


 * *CDS Gene Sequence (paste as text only): **<span style="background-color: #ffffff; font-family: arial,helvetica,clean,sans-serif;">NC_000913.3

code <span style="background-color: #ffffff; font-family: monospace,serif;"><span class="ff_line">ATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAA AAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCC AAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAA GACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGA TTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTA TGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCGAT GTACTGGCGCGCACCTGCGATCCAACCGATCGTGGGACTATTATTATTTTTGGCGATGGCGCGGGCGCTG CGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGTTATGGTGA ATTGCTGACGCTGCCAAACGCCGACCGCGTGAATCCAGAGAATTCAATTCATCTGACGATGGCGGGCAAC GAAGTCTTCAAGGTTGCGGTAACGGAACTGGCGCACATCGTTGATGAGACGCTGGCGGCGAATAATCTTG ACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAA ACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTC CCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCT TTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAG code


 * *GC% Content for gene: **
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **
 * *GC% Content for gene (codon optimized): **

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)

**
 * Primer design results for 'tail' primers (this is just 2 sequences): **