Target+2-oxoglutarate+synthase,+(Helicobacter+pylori)


 * Target (protein/gene** name):


 * NCBI Gene # or RefSeq#**
 * Protein ID (NP or XP #) or Wolbachia#:**
 * Organism (including strain)** Heliobacter Pylori
 * Etiologic Risk Group (see link below):**
 * Background/Disease Information (sort of like the Intro to your Mini Research Write up):**
 * Essentiality of this protein:**
 * Complex of proteins?:**
 * Druggable Target:**


 * *EC#: 1.2.7.3 **


 * Link to BRENDA EC# page:**


 * --** Show screenshot of BRENDA enzyme mechanism schematic


 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**


 * -- link to Sigma (or other company) page for assay or assay reagents (substrates)**


 * -- link (or citation) to paper that contains assay information**


 * -- List cost and quantity of substrate reagents and supplier**


 * Structure Available (PDB or Homology model)**

-- PDB # or closest PDB entry if using homology model:

-- For Homology Model option:

Show pairwise alignment of your BLASTP search in NCBI against the PDB

Query Coverage:

Max % Identities:

% Positives

Chain used for homology:


 * Current Inhibitors:**


 * Expression Information (has it been expressed in bacterial cells):**


 * Purification Method:**


 * Image of protein (PyMol with features delineated and shown separately):**


 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):**


 * length of your protein in Amino Acids**


 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website**


 * Molar Extinction coefficient of your protein at 280 nm wavelength:**


 * TMpred graph Image ** ( @http://www.ch.embnet.org/software/TMPRED_form.html ). Input your amino acid sequence to it.


 * CDS Gene Sequence (paste as text only):**


 * GC% Content for gene:**


 * CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):**


 * GC% Content for gene (codon optimized):**

Do Not Need this info for Spring (but still copy these lines to your Target page for now)


 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**


 * ( link to DNA Works output text file - **that should be saved in your Google Docs folder after you did the primer design protocol)

-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.