Daily+Schedule+Summ12

A tentative schedule of things we will do over the first few weeks. Aldo, Andrew, Michael, Daniel, Janice, Max, Urvashi, Alex, Kaarthik, Aakash Rishi, Sam, Paul, Priya
 * Researcher's Names**:

NOTE - see bottom of page for previous weeks' schedules and things to catch up on.

This Week

 * MONDAY 30-Jul**

2:30 - **Lab Group Meeting**/Journal Club (in WELCH classroom) Final Summer Research Presentations (__upload to Google Docs by Sunday night__)
 * 2-3 slides, ~ 7 minutes
 * What your disease and protein target are (since the rest of the group doesn't know exactly what you are working on)
 * Specifics about size of gene, GC% content, etc.
 * Size of protein, is there a structure in the PDB?
 * What you set out to do
 * Where you have gotten so far (show gel pics, images, graphs or relevant data)
 * Challenges, failures, side-steps
 * Future Directions (i.e. Fall semester and beyond)

Social ???


 * TUESDAY 31-Jul**


 * WEDNESDAY 1-Aug**

Dr. B - out of town
 * THURSDAY 2-Aug**


 * FRIDAY 3-Aug**

Dr. B - out of town

(Remember to work on your 1 page (~500) word summary of your research. No figures needed. - Motivation, What you did, What you have left to do on your project. Reflect on what you have gotten out of your summer research and if it was what you expected. - submit to Dr. B before Sept. 1st. Then I will proof and send to Dr. Simmons.


 * SATURDAY 4-Aug**

Dr. B - out of town

Dr. B - out of town....
 * SUNDAY 5-Aug**

Next Week
FRIDAY - **August 10th**Last Day of VDS Summer Research

Screening with ICM Homology Modeling DSF (Differential Scanning Fluorimetry) assays qPCR Crystallization trials
 * Cool Stuff We May Not Get To:**
 * pharmacophore models

Transformation of plasmids to make stocks - pUC19 (NOT YET) ProtocolBacterialGrowthCurve_VDS_Summ12.xlsx - test (needs to be modified a little)

Label new reagents and supplies Dilute new 100 bp ladder in Blue Juice + TE test all tubes of __#|Taq Polymerase__ in PCR Target Discovery choices --> order primer sets
 * Staff Stuff:**

Journal Club Lab Notebook Checks Protocol cost analysis (Transformation, cloning) Walk through protein expression (use current mentor's enzymes)
 * ToDo List:**
 * Joey & Christina's //Francisella// HAP enzyme
 * Sadhana's //M. tuberculosis// enzyme
 * Joshua's //M. tuberculosis// enzyme
 * J.C.'s YopH

- New guys - walk through with Dr. B - everyone else - complete 2 new Lab Safety classes - see Required Lab Safety link on the left hand side menu of this page Paper copy of safety __#|training__ to Dr. B
 * Lab Safety training**

4 plates of LB with Amp Make 250 ml of LB

Transform pGBR22 into DH5alpha Do this after a successful transformation: Bacterial Growth Curve.xls

Make Amp and Kan stocks (50ug/ul) 4 people: Transform a plasmid for Midi-prep (pGBR22) rest: Transformation Efficiency

Order 'Tail' Primers for class - PCR Primer Design for pNIC-Bsa4 Cloning.docx

expression, purification, characterization
 * test specific activity of your enzyme for lot to lot consistency

__**Research Presentation Schedule:**__ Date: Name


 * Instructions**: prepare 3-5 Powerpoint slides to present your work to date
 * **Intro (talk about your disease and what your protein does)**, **Objectives**, **Methods& Results**, **Future Directions**

make and run SDS-Page protein gels load and run SDS-Page protein gels


 * MONDAY 6-Aug**


 * TUESDAY 7-Aug**


 * WEDNESDAY 8-Aug**

Week 1
1:00 - PAI 2.14 First meeting Post your class schedule to GDOcs - see Announcements Page Create your own folder in Google Docs under the folder: **/StudentFolders** Protocol Target Discovery
 * THURSDAY 31-May**

Target group meetings with Dr. B - computer lab 'Learn how to submit to DNA sequencing' (starring Joey) **-** wet lab
 * FRIDAY 1-Jun**
 * Simultaneously:**


 * Maybe......**


 * SATURDAY 2-Jun**
 * SUNDAY 3-Jun**

Week 2
12:00 - Target group meeting with Dr. B (//Francisella// group - Aldo, Janice, Jennifer R.)
 * MONDAY 4-Jun**

2:30 - 2nd Group Meeting (Location TBA - start in PAI 2.14) 1st Journal Club - Max M.

Target group meetings with Dr. B - Chagas (T. cruzi), Yersinia pestis,, Wolbachia) Leishmania - work on Primer Design.

Protocol Primer Design OverlapAssembly - con't.... Order Overlap Primers for class - con't....

Print out safety training, hole punch and place in VDS sign in folder under the 'Safety' tab
 * Returning VDSers - have 2 classes to take
 * New guys - have lots of classes to take

1:00 Protocol Primer Design OverlapAssembly - con't.... Order Overlap Primers for class - con't....
 * TUESDAY 5-Jun**

Choose a Plasmid to work with on these next 2 exercises(pGBR22 or pGFP or pmCherry)
 * 1) Nanodrop plasmid DNA (pGBR22 or pGFP or pmCherry)


 * WEDNESDAY 6-Jun**

**On Your Targets Page:**
Add Link to DNA Works output text file Add substrate cost, quantity, and catalog numbers (and supplier) for enzyme assay reagents Add image of BRENDA reaction mechanism (screenshot it)

Check DNA sequence 3:00 - **Analyze DNA sequence exercise** - in WEL computer lab

RE Digest Agarose Gel Journal article for next week is posted on Journal Club page.
 * THURSDAY 7-Jun**


 * FRIDAY 8-Jun**

Safety training check.

Michael - printed out copies of the Journal club article for you to take and read - they are on the front table.

PCR - of pGBR22 Agarose Gel


 * SATURDAY 9-Jun**


 * SUNDAY 10-Jun**

Week 3
complete - Analyze DNA sequence exercise Safety training check.

Gel check PCR from Friday (pGBR22) - repeat PCR if it failed Someone order primers (not our gene ones) with Dr. B and Joey.
 * MONDAY 11-Jun**

Dilute some primers

New guys - pipetting exercise and Buffers & Solutions (Lab 1 of Spring VDS)

High Schoolers - Lab Safety training - pipetting exercise and Buffers & Solutions (Lab 1 of Spring VDS) - Literature search assignment

PCR practice


 * 2:30 pm - Lab Group Meeting in WELCH 3.260** (read journal club article prior)

Choose a Plasmid to work with on these next 2 exercises(pGBR22 or pGFP or pmCherry)
 * 1) Nanodrop plasmid DNA (pGBR22 or pGFP or pmCherry)
 * 2) Transformation Efficiency Protocol (pGBR22 or pGFP or pmCherry)
 * Do this exercise in pairs.

@https://docs.google.com/spreadsheet/viewform?formkey=dGdJaEQ5cUtEQm1uaXJ5LVNNdUR4NkE6MQ
 * TUESDAY 12-Jun**
 * Naturally Obsessed Form** - when to watch this documentary- Please fill out this form....

Check Transformation Efficiency

Repeat 1st PCR if failed (why did it fail?) Then: ProtocolPCRforREcloningGREEN.xls OR ProtocolPCRforREcloningRED.xls
 * PCR practice**

PM - 4 people transform pNIC-Bsa4 in DH5alpha PM - 4 people transform YopH and or PTP1b for protein expression practice (or do Ft Hap, Mt PSTP, etc)

Someone transform pmCherry for their Transformation Efficiency lab - Rishi and Jennifer?

High Schoolers & Newer people - Lab Safety training - make Google Docs folders in StudentFolders - Nanodrop protocol - Submit to DNA Sequencing protocol - pipetting exercise and Buffers & Solutions (Lab 1 of Spring VDS) - Literature search assignment

ProtocolPCRforREcloningGREEN.xls OR ProtocolPCRforREcloningRED.xls
 * WEDNESDAY 13-Jun**
 * PCR - practice -**

?? Protein Expression practice (YopH, PTP1b, Ft HAP, MtPSTP, MtPTPb) - takes ALL day

PCR practice check pNIC-Bsa4 transformation plates grow up pNIC-Bsa4 o/n (overnight) grow up YopH and or PTP1b for protein expression practice

High Schoolers & Newer people - pipetting exercise and Buffers & Solutions (Lab 1 of Spring VDS) - Literature search assignment - Analyze DNA Sequence exercise

Order: 500ml flask clamps, 4L flasks and 4L flask clamps Store overnight LB flasks in Fridge or go straight to 1st spin down step of MidiPrep (can save pellet in freezer until afternoon)
 * THURSDAY 14-Jun**

1:00 Everyone:
 * ProtocolPCR_PrimerDesign_for_pNIC-Bsa4CloningSumm12.doc
 * Pick up primers we ordere 2 days ago
 * ProtocolPrimerDilutionVDS_Summ12.docx

PCRs to do: ProtocolPCRforREcloningGREEN.xls OR ProtocolPCRforREcloningRED.xls ProtocolPCR_pLICsequencing.xls

Spin down and then Midi-prep pNIC-Bsa4 (in pairs) -
 * ProtocolMidiPrepKit_HiSpeed_VDS.doc

Everyone: ProtocolDNASequencingFacilitypNIC-Bsa4_VDS_Summ12.doc

Someone transform pmCherry and pGFP for their Transformation Efficiency lab - Rishi and Jennifer? Paul, Urvashi

?grow up pmCherry and pGFP o/n (overnight) - Urvashi, Rishi, Jennnifer, Andrew, Kaarthik

High Schoolers & Newer people - pipetting exercise and Buffers & Solutions (Lab 1 of Spring VDS) - Literature search assignment - Analyze DNA Sequence exercise - RE digest

Order: 500ml flask clamps, and 4L flask clamps
 * FRIDAY 15-Jun**

LARRY - test all tubes of KOD Polymerases in PCR

For those listed on board:
 * Pick up primers we ordered 2 days ago
 * ProtocolPrimerDilutionVDS_Summ12.docx

ProtocolPCRforREcloningGREEN.xls OR ProtocolPCRforREcloningRED.xls ProtocolPCR_pLICsequencing.xls
 * PCRs to do:**

Spin down and then Midi-prep pNIC-Bsa4 (in pairs) -
 * ProtocolMidiPrepKit_HiSpeed_VDS.doc

Everyone: ProtocolDNASequencingFacilitypNIC-Bsa4_VDS_Summ12.doc

High Schoolers & Newer people - Literature search assignment - Analyze DNA Sequence exercise - RE digest check on Gel


 * SATURDAY 16-Jun**

Dr. B of town
 * SUNDAY 17-Jun**

Week 4
Dr. B of town This is what Dr. B is doing: @http://workshop.eupathdb.org/2012/index.php?page=schedule
 * MONDAY 18-Jun**

Everyone: ProtocolDNASequencingFacilitypNIC-Bsa4_VDS_Summ12.doc (if you did not do on Friday)

ProtocolPCRforREcloningGREEN.xls OR ProtocolPCRforREcloningRED.xls ProtocolPCR_pLICsequencing.xls
 * PCRs to do succesfully before you can move on to Target cloning:**

Veteran VDSers:
 * 1) Pick up target tail primers (Leader and Laggers) we ordered 2 days ago (Joey to get in MBB building)
 * ProtocolPrimerDilutionVDS_Summ12.docx
 * 1) Make your Oligo Mix for your target (first step in Overlap PCR protocol) using PLATE of overlap primers - VERY important to not cross-contaminate your primers!!
 * ProtocolPCR_PrimerOverlapVDS_Summ12.docx
 * takes A LONG time to thaw primers in box.

Transformation Efficiency:
 * pmCherry **Rishi,Faiz, Priya,
 * pGFP ** Bobbi, Paul, Jennifer

Dr. B of town Joey in Ochem Lab Christina doing Intel internship - Larry to work with new guys on PyMol Labs 1 & 2 (Rishi, Jennifer R., Sam, Paul, Bobbi, Priya, Faiz) - Day off for everyone else (Holiday: National Virtual Screening Day!) 6:00 - Lab floors will be getting stripped and waxed. NO ONE IN LAB at this time. Please clean up loose stuff off the floors.
 * TUESDAY 19-Jun**

Dr. B of town Veteran VDSers: New guys: Rishi,Faiz, Priya, Bobbi, Paul, Jennifer, Grow up pmCherry and ** pGFP ** overnight each in 2 x 80 ml of LB - ,Faiz, Priya, pmCherry - Faiz, Priya, Paul Grow up pNIC-Bsa4 for Midiprep tomorrow in 2x 80 ml of LB: Rishi, Urvashi,
 * WEDNESDAY 20-Jun**
 * 1) Overlap PCRs
 * 2) Make Kan/Sucrose LB Agar plates for your target cloning
 * LabBeer'sLawVDS_Spring12.doc (see Google Docs/Protocols/Spring12_Labs/)
 * pGFP - ** Bobbi, Jennifer,

Veteran VDSers:
 * THURSDAY 21-Jun**
 * Make Kan/Sucrose LB Agar plates for your target cloning
 * ProtocolPCRforREcloningGREEN.xls OR ProtocolPCRforREcloningRED.xls
 * ProtocolPCR_pLICsequencing.xls
 * Dilute Tail primers - ProtocolPrimerDilutionVDS_Summ12.docx
 * Overlap PCRs

ProtocolMidiPrepKit_HiSpeed_VDS.doc on pmCherry - Faiz, Priya, Paul pNIC-Bsa4 : Rishi, Urvashi,
 * pGFP - ** Bobbi, Jennifer,

Grow up pNIC-Bsa4 for Midiprep tomorrow in 2x 80 ml of LB: ????, ???? Grow up ** pGFP **

(NOT YET) ProtocolBacterialGrowthCurve_VDS_Summ12.xlsx - test (needs to be modified a little)
 * FRIDAY 22-Jun**

Veteran VDSers:
 * Make Kan/Sucrose LB Agar plates for your target cloning
 * ProtocolPCRforREcloningGREEN.xls OR ProtocolPCRforREcloningRED.xls
 * ProtocolPCR_pLICsequencing.xls
 * Dilute Tail primers - ProtocolPrimerDilutionVDS_Summ12.docx
 * Overlap PCRs

Midiprep on pNIC-Bsa4 : Rishi, Urvashi, After your Primary and Secondary Overlap PCR work, go to : ProtocolpNIC-Bsa4_cloningVDS_Summ12.doc 2:00 - Joey & Dr. B tour the Algae lab


 * SATURDAY 23-Jun**
 * SUNDAY 24-Jun**

5th Week
Naturally Obsessed viewing @https://docs.google.com/spreadsheet/viewform?formkey=dHp6M2hkcE16RlRLcW1wcHNPTXp3Vmc6MA
 * Naturally Obsessed Form 2** - when to watch this documentary- Please __#|fill out__ this form.... for the SECOND time!

The **Naturally Obsessed viewing** will be **Thursday June 28th form 6-7 pm** ?? at **Dr. B's House**

Fill out - **Medical Waiver** (only 1 per person) Fill out - 2 **Liability forms**:
 * 1) Documentary Viewing (at Dr. B's House)
 * 2) TACC Tour

Walk through protein expression (use current mentor's enzymes)
 * Joey & Christina's //Francisella// HAP enzyme
 * Sadhana's //M. tuberculosis// enzyme
 * Joshua's //M. tuberculosis// enzyme
 * J.C.'s YopH

make SDS-Page protein gels for characterization step

2:30 - Lab Group Meeting in WELCH with Journal Club
 * MONDAY 25-Jun**

Veteran VDSers:
 * Make Kan/Sucrose LB Agar plates for your target cloning
 * ProtocolPCRforREcloningGREEN.xls OR ProtocolPCRforREcloningRED.xls
 * ProtocolPCR_pLICsequencing.xls
 * Dilute Tail primers - ProtocolPrimerDilutionVDS_Summ12.docx
 * Overlap PCRs
 * After your Primary and Secondary Overlap PCR work, go to : ProtocolpNIC-Bsa4_cloningVDS_Summ12.doc

Midiprep on pNIC-Bsa4 : Rishi, Urvashi, Midiprep on pUC19 : ??

Transformation of plasmids to make stocks - pUC19

Grow up pNIC-Bsa4 for Midiprep tomorrow in 2x 80 ml of LB: ??

New guys: Rishi,Faiz, Priya, Bobbi, Paul > Analyze DNA Sequence exercise ProtocolPrimerDilutionVDS_Summ12.docx
 * Gel Check 1st PCR
 * LabBeer'sLawVDS_Spring12.doc (see Google Docs/Protocols/Spring12_Labs/)
 * PyMol 2, 3Literature search assignment

Mentors: prepare RE digest products for MSers (Andrew, Alex, Michael, Daniel) - check on your LB and __#|Flasks__ for expression Veteran VDSers - work on cloning Midiprep on pNIC-Bsa4 : Rishi, Urvashi, Janice 6:00 __#|start__ overnight culture for protein expression (Andrew, Alex, Michael, Daniel)
 * TUESDAY 26-Jun**
 * Joey & Christina's //Francisella// HAP enzyme
 * Sadhana's //M. tuberculosis// enzyme

10:30 - all day: protein expression (Andrew, Alex, Michael, Daniel)
 * WEDNESDAY 27-Jun**
 * Joey & Christina's //Francisella// HAP enzyme
 * Sadhana's //M. tuberculosis// enzyme


 * THURSDAY 28-Jun**

Make LB and Autoclave with flasks that you will use. -- Kaarthik prepare LB and Flasks --- put 500ml into in 5x 2L flasks -- put 50 ml into 10 x 250 ml flasks

1:00 - - Urvashi, Janice, Max, Aldo - put LB and flasks in Autoclave (can autoclave directly in flask)

1:00 - Make Protein Gels (Homemade SDS-Page) with Dr. B or Joey - Daniel, Andrew, Alex, Michael

5:00 - Pick colonies and Start o/n protein expression in the 50 ml LB Flaks For each person: 1. one flask at 37degC in air incubator 2. one flask at 30degC in water bath shaker
 * Max - MtPSTP (Sadhana's Tuberculosis)
 * Urvashi - MtPSTP (Sadhana's Tuberculosis)
 * Kaarthik - FtHAP (Joey's and Christina's Francisella)
 * Janice - FtHAP (Joey's and Christina's Francisella)
 * Rishi - FtHAP (Joey's and Christina's Francisella)

5:00 - Janice - grow up some flaks of 2x80ml of LB with pNIC-Bsa4 in Kanamycin for eventual midiprep

Meet at the lab at 5:30 to head over to Dr. B's house Will use cars or can also take the #21 Bus that leaves from Dean Keeton & University @ 5:47 pm The **Naturally Obsessed viewing** will be **Thursday June 28th form 6-7 pm** at **Dr. B's House**

12:30, 1:00 - all day: protein expression (Max, Kaarthik , Urvashi , Janice , Rishi, Aldo (observe) ) -- we will start a little later than we normally would but we will use an abbreviated induction phase (~ 3 hrs) so that we can finish in a more reasonable time. Monitor log phase growth in Excel spreadsheet graph
 * FRIDAY 29-Jun**

Janice - spin down pNIC-Bsa4 pellets. Save for doing Midiprep next week Make Protein Gels (Homemade SDS-Page) with Dr. B or Joey - Alex, Michael - (and if time permits: Max, Kaarthik, Uvashi, Janice, Rishi, Aldo)

High Schoolers and new guys pmCherry PCR pLIC PCR

EVERYONE: determine gene inside pNIC-Bsa4 by checking your pLIC sequencing that you sent to the core the other week


 * SATURDAY 30-Jun**
 * SUNDAY 1-Jul**

(NOT YET) ProtocolBacterialGrowthCurve_VDS_Summ12.xlsx - test (needs to be modified a little)

FRIDAY 21-May SATURDAY 22-May SUNDAY 23-May MONDAY 24-May TUESDAY 25-May

6th Week
EVERYONE: determine gene inside pNIC-Bsa4 by checking your pLIC sequencing that you sent to the core the other week EVERYONE THAT HAS DONE MIDI-Prep (successfully): Have you submitted your sample to sequencing? Does it turn out to be the right plasmid? Veteran VDSers - work on cloning Daniel & Andrew - do test growth of FtHAP and MtPSTP in 50 ml of LB + Kan Each Person: Measure OD every few hours over ~ 8hrs time span until OD reaches 1.000 at 600nm Make LB and Autoclave with flasks that you will use. --- put 500ml into in 2x 2L flasks -- put 50 ml into 4 x 250 ml flasks 2:30 - **Lab Group Meeting**/Journal Club (in WELCH classroom) High Schoolers and new guys (Rishi,Faiz, Priya, Bobbi, Paul): --ProtocolPrimerDilutionVDS_Summ12.docx --pmCherry PCR --pLIC PCR - ProtocolPCR_pLICsequencing.xls --Overlap PCR of target Janice - overnight growth of 2x 80ml of LB with pNIC-Bsa4 colonies for doing Midiprep Make Protein Gels (Homemade SDS-Page) with Dr. B or Joey - Alex, Michael - (and if time permits: Max, Kaarthik, Uvashi, Janice, Rishi, Aldo
 * MONDAY 2-Jul**
 * one flask at 30degC in water bath shaker
 * one flask at 37deg in air incubator

Janice - spin down samples for pNIC-Bsa4 Midiprep Protein Sonication (Andrew, Alex, Michael, Daniel)
 * TUESDAY 3-Jul**

Protein expression - AGAIN Monitor log phase growth in Excel spreadsheet graph make SDS-Page protein gels for characterization step 3:00 - **Algae Lab Tour**: Meet in PAI 2.14 - then will walk over to BIO 320 Janice and Sam - Midiprep pNIC-Bsa4 plasmid samples, Nanodrop them and then send to sequencing
 * Joey & Christina's //Francisella// HAP enzyme
 * Sadhana's //M. tuberculosis// enzyme

OFF - Independence Day
 * WEDNESDAY 4-Jul**

EVERYONE: determine gene inside pNIC-Bsa4 by checking your pLIC sequencing that you sent to the core the other week EVERYONE THAT HAS DONE MIDI-Prep (successfully): Have you submitted your sample to sequencing? Does it turn out to be the right plasmid? ~ 10:00 Prep Starter Culture (grow until ~ 8hrs to OD600 = 1 ) Protein Expression People also make LB media and autoclave Protein Sonication (Andrew, Alex, Michael, Daniel ) Janice and Sam - send Midiprep sample of pNIC-Bsa4 to sequencing ~ 6:00 Move Starter Cultureto store in 4degC
 * THURSDAY 5-Jul**
 * Max - MtPSTP (Sadhana's Tuberculosis)
 * Urvashi - MtPSTP (Sadhana's Tuberculosis)
 * Kaarthik - FtHAP (Joey's and Christina's Francisella)
 * Janice - FtHAP (Joey's and Christina's Francisella)
 * Aldo- FtHAP (Joey's and Christina's Francisella)
 * Max - MtPSTP (Sadhana's Tuberculosis)
 * Urvashi - MtPSTP (Sadhana's Tuberculosis)
 * Kaarthik - FtHAP (Joey's and Christina's Francisella)
 * Janice - FtHAP (Joey's and Christina's Francisella)
 * Aldo- FtHAP (Joey's and Christina's Francisella)

Protein Purification (Andrew, Alex, Michael, Daniel ) --- Run gels, stain and destain
 * FRIDAY 6-Jul**

Protein expression 11:00 ? - talk to Dr. B if you can't make this time
 * Max - MtPSTP (Sadhana's Tuberculosis)
 * Urvashi - MtPSTP (Sadhana's Tuberculosis)
 * Kaarthik - FtHAP (Joey's and Christina's Francisella)
 * Janice - FtHAP (Joey's and Christina's Francisella)
 * Aldo- FtHAP (Joey's and Christina's Francisella)

make SDS-Page protein gels for characterization step

WEDNESDAY 26-May THURSDAY 27-May FRIDAY 28-May
 * SATURDAY 7-Jul**
 * SUNDAY 8-Jul**

TACC Tour - Thursday July 12th @3:00
(meet in lab before - then we'll catch bus up to __Pickle campus__) Fill out - **Medical Waiver** Fill out - **Liability form**

~ 10:00 Prep Starter Culture (grow until ~ 8hrs to OD600 = 1 )
 * MONDAY 9-Jul**
 * Max - MtPSTP (Sadhana's Tuberculosis)
 * Aldo - do protein expression starter culture too.

2:30 - **Lab Group Meeting**/Journal Club (in WELCH classroom) ~ 6:00 Move Starter Cultureto store in 4degC
 * Max - MtPSTP (Sadhana's Tuberculosis)
 * Aldo

Protein expression 11:00 ? -
 * TUESDAY 10-Jul**
 * Max - MtPSTP (Sadhana's Tuberculosis)
 * Aldo


 * WEDNESDAY 11-Jul**

NOTE - not change in schedule

TACC Tour at VisLab in ACES building __ON CAMPUS__ - @3:00
(meet in lab before at 2:50to walk over)


 * THURSDAY 12-Jul**

TACC Tour - @3:00 Cancelled and moved to WED
(meet in lab before - then we'll catch bus up to Pickle campus)


 * FRIDAY 13-Jul**


 * SATURDAY 14-Jul**


 * SUNDAY 15-Jul**


 * MONDAY 16-Jul**

2:30 - **Lab Group Meeting**/Journal Club (in WELCH classroom)


 * TUESDAY 17-Jul**


 * WEDNESDAY 18-Jul**


 * THURSDAY 19-Jul**


 * FRIDAY 20-Jul**


 * SATURDAY 21-Jul**


 * SUNDAY 22-Jul**


 * MONDAY 23-Jul**

2:30 - **Lab Group Meeting**/Journal Club (in WELCH classroom)


 * TUESDAY 24-Jul**


 * WEDNESDAY 25-Jul**

2-4 pm - Middle Schoolers visit lab


 * THURSDAY 26-Jul**

072612 - Thursday 3:00 - GASF (Genome Sequencing and Analysis Facility) Tour in MBB 4.102 Paper to read before the tour:

– Meet in lab at 2:50


 * FRIDAY 27-Jul**


 * SATURDAY 28-Jul**

SATURDAY 29-May SUNDAY 30-May MONDAY 31-May TUESDAY 1-Jun WEDNESDAY 2-Jun THURSDAY 3-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">FRIDAY 4-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SATURDAY 5-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SUNDAY 6-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">MONDAY 7-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">TUESDAY 8-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">WEDNESDAY 9-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">THURSDAY 10-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">FRIDAY 11-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SATURDAY 12-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SUNDAY 13-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">MONDAY 14-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">TUESDAY 15-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">WEDNESDAY 16-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">THURSDAY 17-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">FRIDAY 18-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SATURDAY 19-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SUNDAY 20-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">MONDAY 21-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">TUESDAY 22-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">WEDNESDAY 23-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">THURSDAY 24-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">FRIDAY 25-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SATURDAY 26-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SUNDAY 27-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">MONDAY 28-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">TUESDAY 29-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">WEDNESDAY 30-Jun <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">THURSDAY 1-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">FRIDAY 2-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SATURDAY 3-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SUNDAY 4-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">MONDAY 5-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">TUESDAY 6-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">WEDNESDAY 7-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">THURSDAY 8-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">FRIDAY 9-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SATURDAY 10-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SUNDAY 11-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">MONDAY 12-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">TUESDAY 13-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">WEDNESDAY 14-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">THURSDAY 15-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">FRIDAY 16-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SATURDAY 17-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SUNDAY 18-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">MONDAY 19-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">TUESDAY 20-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">WEDNESDAY 21-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">THURSDAY 22-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">FRIDAY 23-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SATURDAY 24-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SUNDAY 25-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">MONDAY 26-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">TUESDAY 27-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">WEDNESDAY 28-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">THURSDAY 29-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">FRIDAY 30-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SATURDAY 31-Jul <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SUNDAY 1-Aug <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">MONDAY 2-Aug <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">TUESDAY 3-Aug <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">WEDNESDAY 4-Aug <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">THURSDAY 5-Aug <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">FRIDAY 6-Aug <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SATURDAY 7-Aug <span style="display: block; height: 1px; left: -40px; overflow: hidden; position: absolute; top: 11557.5px; width: 1px;">SUNDAY 8-Aug
 * SUNDAY 29-Jul**