Hypoxanthine+phosphoribosyltransferase+(Mycobacterium+tuberbulosis)

[] [|https://www.patricbrc.org/view/Feature/PATRIC.83332.12.NC_000962.CDS.4063254.4063856.rev#view_tab=overview] []
 * Target (protein/gene name): ** Hypoxanthine guanine phophoribosyltransferase (HGPTR)
 * NCBI Gene # or RefSeq#: **NC_000962.3
 * Protein ID (NP or XP #) or Wolbachia#: ** NP_218141
 * Organism (including strain): ** //Mycobacterium tuberculosis (H37Rv)//
 * Etiologic Risk Group (see link below): ** N/A
 * Disease Information (sort of like the Intro to your Mini Research Write up): ** //Mycobacterium tuberculosis// is a pathogenic bacteria responsible for the infectious disease called Tuberculosis. Tuberculosis is highly virulent, having a high morbidity and mortality rate compared to other diseases. The disease began increasing after 1985 when HIV infections were introduced that lowered the immunity of the body. The virulence of the bacteria is transferred by microscopic airborne bodily fluids that infect the alveolar surfaces in the lungs. Some common symptoms include coughing, chest pain, fatigue, fever and etc. People who live in areas that lack medical care, have poor nutritional resources, and are overcrowded risk exposure to Tuberculosis. Each year, mutations have lead to more resistant strains of //M.// tuberculosis, so research is always ongoing to counteract the spreading of the disease.
 * Link to TDR Targets page (if present): **
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) **

Is it a monomer or multimer as biological unit?: The most probable structure of HGPTR is a dimer.
 * Essentiality of this protein: **

HGPTR plays a role in nucleotide salvage pathways. This protein is essential in //M. tuberculosis// and other organisms for the recycling of the purine nucleotide for DNA replication and transcription. Salvaging nucleotides will allow the infectious organism to replicate its genetic material efficiently to be carried out in a viral pathway in the host. There were also essentiality data on TDR targets that described HGPTR as gaining significant fitness.

[1] Eng, W.S.; Hockova, D.; Spacek P.; Janeba, Z.; West, N.P.; Woods, K.; Naesens, L.M.J.; Keough, D.T.; Guddat, L.W. First Crystal Structures of //Mycobacterium tuberculosis// 6-Oxopurine Phosphoribosyltransferase: Complexes with GMP and Pyrophosphate and with Acyclic Nucleoside Phosphonates Whose Prodrugs Have Antituberculosis Activity. //Journal of Medicinal Chemistry// **2015**, 58, (11), 4822-4838
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **

[2] []

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 * EC#: ** 2.4.2.8
 * Link to BRENDA EC# page: **

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 * Link to BRENDA HPTR Pathway: **

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 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **

-- PDB # or closest PDB entry if using homology model: 5KNP
 * Structure (PDB or Homology model) **

Biazus, G.; Schneider, C.Z.; Palma, M.S.; Basso, L.A.; Santos, D.S. Hypoxanthine-guanine phosphoribosyltransferase from //Mycobacterium tuberculosis// H37Rv: Cloning, expression, and biochemical characterization. //Protein Expression and Purification// **2009**, 66, (2), 185-190
 * Current Inhibitors: ** N/A in //M. tuberculosis//
 * Expression Information (has it been expressed in bacterial cells): **

Fig 1. Crystal structure of Mycobacterium tuberculosis hypoxanthine guanine phophoribosyltransferase (5KNP) in complex with [3S,4R]-(4-(Hypoxanthin-9-yl)pyrrolidin-3-yl)-oxymethanephosphonic acid
 * Image of protein (PyMol with features delineated and shown separately): **

code >5KNP:A|PDBID|CHAIN|SEQUENCE HHHHHHHVTQSSSAITPGQTAELYPGDIKSVLLTAEQIQARIAELGEQIGNDYRELSATTGQDLLLITVLKGAVLFVTDL ARAIPVPTQFEFMAVSSYGSSTSSSGVVRILKDLDRDIHGRDVLIVEDVVDSGLTLSWLSRNLTSRNPRSLRVCTLLRKP DAVHANVEIAYVGFDIPNDFVVGYGLDYDERYRDLSYIGTLDPRVYQ code 207 AA 22.94284 kDa 18910 Fig 2. TMpred output for hypoxanthine guanine phophoribosyltransferase
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **
 * length of your protein in Amino Acids **
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website **
 * Molar Extinction coefficient of your protein at 280 nm wavelength: **
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.

code >NC_000962.3:c4063904-4063254 Mycobacterium tuberculosis H37Rv, complete genome TTGACGCCCGCGTTGGTCGTTGGTCCCGCGGCGTGGCACGCTGTGCACGTGACCCAGAGCTCCTCGGCGA TCACCCCCGGGCAGACGGCGGAGCTTTATCCGGGGGACATCAAGTCGGTGCTGCTCACGGCCGAGCAGAT TCAGGCCCGCATCGCCGAGCTCGGCGAGCAGATCGGCAACGACTACCGCGAGCTGTCCGCTACCACCGGC CAGGATCTGCTGCTGATCACCGTGCTGAAGGGCGCGGTGCTCTTCGTCACCGACCTGGCGCGAGCGATTC CCGTGCCGACCCAGTTCGAGTTCATGGCGGTGAGTTCGTATGGGTCATCGACATCCTCGTCGGGCGTGGT GCGGATCCTCAAGGACCTCGACCGCGACATCCACGGCCGCGACGTGCTGATCGTCGAGGACGTCGTCGAC TCCGGCCTTACGCTTTCGTGGTTGTCGCGGAACCTGACGAGCCGGAATCCGCGGTCATTGCGGGTGTGCA CGCTGCTGCGCAAGCCCGATGCGGTGCACGCCAACGTCGAAATCGCGTACGTGGGTTTCGACATTCCCAA CGACTTCGTCGTGGGCTACGGCCTGGACTACGACGAACGCTACCGTGACCTGTCATACATCGGGACGCTG GACCCCAGGGTCTATCAGTAG code
 * CDS Gene Sequence (paste as text only): **

code >NP_218141.1 hypoxanthine-guanine phosphoribosyltransferase [Mycobacterium tuberculosis H37Rv] MTPALVVGPAAWHAVHVTQSSSAITPGQTAELYPGDIKSVLLTAEQIQARIAELGEQIGNDYRELSATTG QDLLLITVLKGAVLFVTDLARAIPVPTQFEFMAVSSYGSSTSSSGVVRILKDLDRDIHGRDVLIVEDVVD SGLTLSWLSRNLTSRNPRSLRVCTLLRKPDAVHANVEIAYVGFDIPNDFVVGYGLDYDERYRDLSYIGTL DPRVYQ code
 * Protein Sequence (paste as text only): **

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)

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 * Primer design results for 'tail' primers (this is just 2 sequences): **