Research+Pages+-+Sonny


 * Sonny's Research**

//**ToDo:**// safety training (see Required Safety Training link to the left) (print out TxClass training history sheet and hole punch and plate into VDS sign in binder with others)

Get a lab notebook

make 250 ml of LB make 4 LB/Agar +AMP plates

Nanodrop plasmid Submit pGBR22 plasmid to DNA sequencing

Transformation efficiency

Analyze DNA sequence exercise (on computer)

RE digest and Agarose gel check

1st PCR - of pGBR22 2nd PCR - Red or Green PCR 3rd PCR - pLIC

Start Cloning of gene for Target

PASTE YOUR RESULTS IMAGES WITH CAPTIONS HERE (and include all in lab notebook):

Transformation Efficiency





Submitting DNA to DNA Sequencing Facility- Results Sonny Cabrera (sc38763) Wednesday, 28, 2011 pGBR22 Forward Sequence Submitted Analysis to Google Docs PCR Protocol: Trial 1 SP6/T7

PCR Protocol :Trial 2 SP6/T7

PCR Protocol: Trial 3 M13 For/Rev

PRC Protocol: Trial 4 SP6/T7 (Success)

Protocol of PCR for RE Cloning Trial 1

Protocol of PCR for RE Cloning Trial 2

Protocol of PCR for RE Cloning Trial 3

Protocol of PCR for RE Cloning Trial 4

Protocol of PCR for RE Cloning Trial 5

Protocol of PCR for RE Cloning Trial 6 (Possible sucess)

PRC Protocol for pLIC Seqencing vectors of pNIC-Bsa4 Trial 1 Results for Trials 1&2 of the PCR Protocol for PLIC Sequencing Vectors pNIC-Bsa4 could be as a result of poor mixing in the dilutions.

PRC Protocol for pLIC Seqencing vectors of pNIC-Bsa4 Trial 1

PRC Protocol for pLIC Seqencing vectors of pNIC-Bsa4 Trial 2 Success

Sonny - what are each of these lanes? - Dr. B

Protocol of PCR for pNIC-Bsa4 Cloning Trial 1 (Success)

Trial 2

Trial 3 PCR's at regular protocol requirements work well. Past three attempts at regular protocol turn out well. All attempts at multiplying the samples to 50ul have resulted in a unsuccessful result.

FALL FRI 2011

PCR Primer Design for Overlap Assembly - 10.14.11