Target+-+2-oxoglutarate+dehydrogenase+complex+(Wolbachia)


 * Target (protein/gene name):** 2-oxoglutarate dehydrogenase complex
 * NCBI Gene # or RefSeq#:** 3266428
 * Protein ID (NP or XP #) or Wolbachia#:** Q5GSP1
 * Organism:** Wolbachia
 * Etiologic Risk Group (see link below):** N/A
 * Background/Disease Information:**
 * Essentiality of this protein:** http://tdrtargets.org/targets/view?gene_id=232973
 * Complex of enzymes:**
 * EC#:** 1.2.4.2
 * Link to BRENDA EC# page:** http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.2.4.2
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**
 * -- link to Sigma (or other company) page for assay or assay reagents (substrates)**
 * -- link to paper that contains assay information**
 * Structure Available (PDB or Homology model):** http://www.rcsb.org/pdb/explore/explore.do?structureId=2JGD
 * -- PDB # or closest PDB entry if using homology model:** 2JGD
 * -- Query Coverage (if not direct match):** 98%
 * -- Max Ident (if not direct match):** 42%
 * Druggable Target (see Databases for this):**
 * Current Inhibitors:**
 * Expression Information (has it been expressed in bacterial cells):**
 * Purification Method:**
 * Image of protein (PyMol or etc): [[image:2jgd_bio_r_500.jpg]]**
 * Amino Acid Sequence:** 887 Wolbachia; 933 E. Coli
 * length of your protein in Amino Acids**
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website:** E. Coli: 105,062 Da Wolbachia: 100,186 Da
 * Molar Extinction coefficient of your protein at 280 nm wavelength:**
 * CDS Gene Sequence:**
 * GC% Content for gene:**
 * CDS Gene Sequence (codon optimized) - after having done Primer Design:**
 * GC% Content for gene (codon optimized):**


 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**


 * Primer design results for 'tail' primers (this is just 2 sequences):**

http://www.uniprot.org/uniprot/P0AFG3
 * E. Coli:**

http://www.uniprot.org/uniprot/Q5GSP1
 * Wolbachia:**