TargetSp17-+Phophoglycerate+Kinase+(Mycobacterium+tuberculosis)

General Information:
MSVANLKDLL AEGVSGRGVL VRSDLNVPLD EDGTITDAGR IIASAPTLKA LLDADAKVVV AAHLGRPKDG PDPTLSLAPV AVALGEQLGR HVQLAGDVVG ADALARAEGL TGGDILLLEN IRFDKRETSK NDDDRRALAK QLVELVGTGG VFVSDGFGVV HRKQASVYDI ATLLPHYAGT LVADEMRVLE QLTSSTQRPY AVVLGGSKVS DKLGVIESLA TKADSIVIGG GMCFTFLAAQ GFSVGTSLLE DDMIEVCRGL LETYHDVLRL PVDLVVTEKF AADSPPQTVD VGAVPNGLMG LDIGPGSIKR FSTLLSNAGT IFWNGPMGVF EFPAYAAGTR GVAEAIVAAT GKGAFSVVGG GDSAAAVRAM NIPEGAFSHI STGGGASLEY LEGKTLPGIE VLSREQPTGG VL
 * Organism: ** Mycobacterium tuberculosis
 * Protein: ** Phosphoglycerate Kinase
 * EC Number ** **:** 2.7.2.3
 * Sequence: **
 * Length of your protein in Amino Acids: ** 461 amino acids.
 * Molecular Weight of your protein in kiloDaltons: ** ~45.0 kDA
 * Molar Extinction coefficient of your protein at 280 nm wavelength: **
 * TMpred graph Image: **

[]

[|http://www.brenda-enzymes.org/enzyme.php?ecno=2.7.2.3&organism_list=Homo%20sapiens&Suchword=&showtm=1&UniProtAcc=&reference=#SUBSTRATE]
 * Link to BRENDA EC# page: **

The BRENDA data shows for Homo Sapien phosphoglycerate kinase. There were no options/data for phosphoglycerate kinase for Mycobacterium tuberculosis.

Mycobacterium Tuberculosis is a bacteria that causes Tuberculosis (TB), a contagious and often airborne disease, mainly affecting the lungs. Main symptoms include a bad cough that may last up to/over 3 weeks, pain in chest, and coughing up of blood or mucus from lungs. Other mild symptoms include fatigue, fever, weight loss, etc. TB is one of the major causes of disability and death worldwide, especially in low and middle income countries.
 * Disease Information: **

[] https://www.niaid.nih.gov/diseases-conditions/tuberculosis-tb

Essentiality:
This protein is involved in the second step of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate in Glycolysis. PGK is found in all living organisms and has been highly conserved throughout evolution. This highlights how essential this protein is.

https://www.ncbi.nlm.nih.gov/pubmed/9384563 http://www.uniprot.org/uniprot/P9WID1#function

Assayable Enzyme Target:
Link goes to known assays based on E.C. number: http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/learning-center/assay-library/enzyme-activator-assays.html

Structure Availability:
Snapshot of phosphoglycerate kinase in Mycobacterium tuberculosis. Following link goes to interactive model of this enzyme: []

There were no pdb pages for this enzyme for Mycobacterium tuberculosis.

Similarity to Human:
After running BLASTP with the amino acid sequence of phophosglycerate kinase of M. Tuberculosis, it matched 98% to human phosphoglycerate kinase. As mentioned previously, this enzyme is in all living organisms, so this finding is not surprising. The human protein has an accession number of: NP_000282.1

https://blast.ncbi.nlm.nih.gov/Blast.cgi#alnHdr_4505763

Current Inhibitors:
Searching for inhibitors through Binding BD, there was only one compound found under the target. This indicates that not the protein has not been studied as a target to a large degree thereby a good candidate for virtual screening.

Expression:
This gene is highly-expressed at all times. It is even more expressed when subject to heat shock or cells are growing in glucose.This gene can also be expressed by bacteria such as E. Coli; reiterating to the essentiality portion above, this enzyme is found in almost all living organisms.