Target-Epoxide+hydrolase+(Mycobacterium+tuberculosis)


 * *Target (protein/gene name): **
 * *NCBI Gene # or RefSeq#: **
 * *Protein ID (NP or XP #) or Wolbachia#: **
 * *Organism (including strain): **
 * Etiologic Risk Group (see link below): **
 * *Background/Disease Information (sort of like the Intro to your Mini Research Write up): **
 * Essentiality of this protein: **
 * Complex of proteins?: **
 * Druggable Target: **

-- PDB # or closest PDB entry if using homology model: -- For Homology Model option: Show pairwise alignment of your BLASTP search in NCBI against the PDB Query Coverage: Max % Identities: % Positives Chain used for homology:
 * *EC#: **
 * Link to BRENDA EC# page: **
 * -- ** Show screenshot of BRENDA enzyme mechanism schematic
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **
 * -- link to Sigma (or other company) page for assay or assay reagents (substrates) **
 * -- link (or citation) to paper that contains assay information **
 * -- List cost and quantity of substrate reagents and supplier **
 * Structure Available (PDB or Homology model) **


 * Current Inhibitors: **
 * Expression Information (has it been expressed in bacterial cells): **
 * Purification Method: **
 * Image of protein (PyMol with features delineated and shown separately): **
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **
 * *length of your protein in Amino Acids **
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website **
 * Molar Extinction coefficient of your protein at 280 nm wavelength: **
 * TMpred graph Image ** ( [] ). Input your amino acid sequence to it.
 * *CDS Gene Sequence (paste as text only): **
 * *GC% Content for gene: **
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **
 * *GC% Content for gene (codon optimized): **

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences): **