Target+-+UDP-N-acetylenolpyruvylglucosamine+reductase

The enzymes involved in peptidoglycan biosynthesis are among the best-known targets in the search for new antibiotics" - Yang, et. al.
 * Target (protein/gene name):** UDP-N-acetylenolpyruvylglucosamine reductase
 * NCBI Gene # or RefSeq#:** 931443
 * Locus Tag:** murB
 * Protein ID (NP or XP #) or Wolbachia#:** YP_816689.1
 * Organism (including strain):** //Streptococcus pneumoniae//
 * Etiologic Risk Group (see link below):** Risk Group 2
 * Background/Disease Information (sort of like the Intro to your Mini Research Write up):**
 * Essentiality of this protein:** Is essential.
 * Complex of proteins?:** No
 * Druggable Target:** Yes "
 * *EC#: 1.1.1.158 **
 * Link to BRENDA EC# page:** []
 * --** Show screenshot of BRENDA enzyme mechanism schematic

Tris-HCl (pH 8.0), 10 mM KCl, 100µ M NADPH, and 50 µ M enolpyruvyl-UDP-N-acetylglucosamine(EP-UNAG) [|NADPH] [|EP-UNAG] [|Yang, Y.; Severin, A.; Chopra, R.; Krishnamurthy, G.; Sing, G.; Hu, W., Keeney.; Svenson, K.; Petersen, P.; Labthavikul, P.; Shales, D.; Rasmussen, B.; Failli, A.; Shumsky, J.; Kutterer, K.; Gilbert, A.; Mansour, T., 3,5-Dioxopyrazolidines, Novel Inhibitors of Acetylenolpyruvylglucosamine-N-UDP Reductase (MurB) with Activity against Gram-Positive Bacteria. Antimicrob. Agents Chemother. 2006, 50(2), 556.] NADPH: $66.20 per 25mg EP-UNAG: $51.40 per 25mg EP-UNAG was synthesized and purified according to the published method - Yang, et. al. -- PDB # or closest PDB entry if using homology model: 1MBT -- For Homology Model option: Show pairwise alignment of your BLASTP search in NCBI against the PDB Top: UDP-N-acetylenolpyruvylglucosamine reductase in //S. pneumoniae// Bottom: UDP-N-acetylenolpyruvylglucosamine reductase in //E. coli//
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **
 * -- link to Sigma (or other company) page for assay or assay reagents (substrates)**
 * -- link (or citation) to paper that contains assay information **
 * -- List cost and quantity of substrate reagents and supplier**
 * Structure Available (PDB or Homology model)**

Query Coverage: 94% Max % Identities:52% % Positives: 68% Chain used for homology: Chain A

Image of protein (PyMol with features delineated and shown separately): MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRFELARVVKFANQENIPWMVLGNASNII VRDGGIRGFVILCDKLNNVSVDGYTIEAEAGANLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAY GGEIAHILQSCKVLTKDGEIETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLR ELKQPLEYPSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQSVIE KVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR
 * Current Inhibitors:** 3,5-Dioxopyrazolidines
 * Expression Information (has it been expressed in bacterial cells):** Yes
 * Purification Method:** ADP-Sepharose column elution purification [|[Details]]
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):**

6,220 cm^-1M^-1
 * length of your protein in Amino Acids:** 316
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website:** 34.5387kDa
 * Molar Extinction coefficient of your protein at 280 nm wavelength:**
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.

TMpred indicates that the protein has a high likelihood of being transmembrane. However, empirical studies indicate that the protein is not transmembrane, but globular and the Philius prediction model concurs.



ATGTCTGTAAGAGAAAAAATGCTTGAAATCTTAGAAGGAATTGATATCCGTTTTAAGGAACCCTTGCATA GCTATAGTTATACAAAAGTAGGTGGAGAGGCTGATTATTTGGTCTTTCCACGAAATCGTTTTGAGTTGGC TCGCGTTGTGAAATTTGCCAACCAAGAAAATATCCCTTGGATGGTTCTTGGCAATGCAAGCAATATCATC GTTCGTGATGGTGGGATTCGTGGATTTGTCATCTTGTGTGACAAGCTCAATAACGTTTCTGTTGATGGCT ATACCATTGAAGCAGAAGCTGGGGCTAACTTGATTGAAACAACTCGCATTGCCCTCCGTCATAGTTTAAC TGGCTTTGAGTTTGCTTGTGGTATTCCAGGAAGCGTTGGCGGTGCTGTCTTTATGAATGCGGGTGCCTAT GGTGGCGAGATTGCTCACATCTTGCAGTCTTGTAAGGTCTTGACCAAGGATGGAGAAATCGAAACCCTGT CTGCTAAAGACTTGGCTTTTGGTTACCGTCATTCAGCTATTCAGGAGTCTGGTGCAGTTGTCTTGTCAGT TAAATTTGCCCTAGCTCCAGGAACCCATCAGGTTATCAAGCAGGAAATGGACCGCTTGACGCACCTACGT GAACTCAAGCAACCTTTGGAATACCCATCTTGTGGCTCGGTCTTTAAGCGTCCAGTCGGGCATTTTGCAG GTCAGTTAATTTCAGAAGCTGGCTTGAAAGGCTATCGTATCGGTGGCGTAGAAGTGTCAGAAAAGCATGC AGGATTTATGATCAATGTCGCAGATGGAACGGCCAAAGACTACGAGGACTTGATCCAATCGGTTATCGAA AAAGTCAAGGAACACTCAGGTATTACGCTTGAAAGAGAAGTCCGGATCTTGGGTGAAAGCCTATCGGTAG CGAAGATGTATGCAGGTGGTTTTACTCCCTGCAAGAGGTAG
 * CDS Gene Sequence (paste as text only):**


 * GC% Content for gene:** 45% [Found Using [|Genomics Content Calculator]]
 * CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):**
 * GC% Content for gene (codon optimized):**

[|-http://brenda-enzymes.org/php/result_flat.php4?ecno=1.1.1.158]
 * Reference Sites:**

-[] [|-http://www.sigmaaldrich.com/catalog/search?interface=All&term=enolpyruvyl-UDP-N-acetylglucosamine&lang=en&region=US&focus=product&N=0+220003048+219853269+219853286] [|-http://aac.asm.org/content/50/2/556#ref-list-1] [|-http://web.expasy.org/protparam/] [|-http://www.ch.embnet.org/software/TMPRED_form.html] [|-http://www.sciencebuddies.org/science-fair-projects/project_ideas/Genom_GC_Calculator.shtml]