Timeline+Vets+F12

TIMELINE FOR RESEARCH (VETS) A tentative schedule of things we will do over the first few weeks. Summer **Veterans**: Aldo, Andrew, Michael, Daniel, Janice, Max, Urvashi, Alex, Kaarthik, Aakash,
 * Researcher's Names**:
 * Sidestreamers**: Rishi, Paul, Priya, Vanna, Keyur, Ben
 * Springers**:

NOTE - see bottom of page for previous weeks' schedules and things to catch up on.

This Week
Finish cloning your gene into pNIC-Bsa4 Transform positive clone with your gene into BL21(De3) cells - begin expression PyMol Refresher - Due by ?? Science Works assignment due ??


 * 27-Aug Monday**


 * 28-Aug Tuesday**

2 - 3pm - **Lab Group Meeting**/Journal Club (in ???? classroom)
 * 29-Aug Wednesday**


 * 30-Aug Thursday**

Virtual Screen Refresher - Due ??
 * 31-Aug Friday**
 * Help Sessions: PyMol and Virtual Screen Refresher Welch Computer Lab

Wikispaces check on each Friday


 * 1-Sep Saturday**
 * 2-Sep Sunday**

Next Week

 * 3-Sep Monday**


 * 4-Sep Tuesday**

2 - 3pm - **Lab Group Meeting**/Journal Club (in ???? classroom)
 * 5-Sep Wednesday**


 * 6-Sep Thursday**

Lab Notebook Check this Friday- leave your notebook in 'Drug Screening Notebooks' drawer in lab by 7:00 pm
 * 7-Sep Friday**
 * should include all current results and commentary

Wikispaces check on each Friday


 * 8-Sep Saturday**
 * 9-Sep Sunday**

Begin **real** Virtual Screening - prepare protein in GOLD and start jobs (cb306 should be done this week and the next one started)


 * 10-SepMonday**
 * 11-SepTuesday**
 * 12-SepWednesday**
 * 13-SepThursday**
 * 14-SepFriday**
 * 15-SepSaturday**
 * 16-SepSunday**
 * 17-SepMonday**
 * 18-SepTuesday**
 * 19-SepWednesday**
 * 20-SepThursday**
 * 21-SepFriday**
 * 22-SepSaturday**
 * 23-SepSunday**
 * 24-SepMonday**
 * 25-SepTuesday**
 * 26-SepWednesday**
 * 27-SepThursday**
 * 28-SepFriday**
 * 29-SepSaturday**
 * 30-SepSunday**


 * 1-Oct Monday**
 * 2-Oct Tuesday**
 * 3-Oct Wednesday**
 * 4-Oct Thursday**
 * 5-Oct Friday**
 * 6-Oct Saturday**
 * 7-Oct Sunday**
 * 8-Oct Monday**
 * 9-Oct Tuesday**
 * 10-OctWednesday**
 * 11-OctThursday**
 * 12-OctFriday**
 * 13-OctSaturday**
 * 14-OctSunday**
 * 15-OctMonday**
 * 16-OctTuesday**
 * 17-OctWednesday**
 * 18-OctThursday**
 * 19-OctFriday**
 * 20-OctSaturday**
 * 21-OctSunday**
 * 22-OctMonday**
 * 23-OctTuesday**
 * 24-OctWednesday**
 * 25-OctThursday**
 * 26-OctFriday**
 * 27-OctSaturday**
 * 28-OctSunday**
 * 29-OctMonday**
 * 30-OctTuesday**
 * 31-OctWednesday**
 * 1-Nov Thursday**
 * 2-Nov Friday**
 * 3-Nov Saturday**
 * 4-Nov Sunday**
 * 5-Nov Monday**
 * 6-Nov Tuesday**
 * 7-Nov Wednesday**
 * 8-Nov Thursday**
 * 9-Nov Friday**
 * 10-NovSaturday**
 * 11-Nov Sunday**
 * 12-Nov Monday**
 * 13-Nov Tuesday**
 * 14-Nov Wednesday**
 * 15-Nov Thursday**
 * 16-Nov Friday**
 * 17-Nov Saturday**
 * 18-Nov Sunday**
 * 19-Nov Monday**
 * 20-Nov Tuesday**
 * 21-Nov Wednesday**
 * 22-Nov Thursday**
 * 23-Nov Friday**
 * 24-Nov Saturday**
 * 25-Nov Sunday**
 * 26-Nov Monday**
 * 27-Nov Tuesday**
 * 28-Nov Wednesday**
 * 29-Nov Thursday**
 * 30-Nov Friday**
 * 1-Dec Saturday**
 * 2-Dec Sunday**
 * 3-Dec Monday**
 * 4-Dec Tuesday**
 * 5-Dec Wednesday**
 * 6-Dec Thursday**
 * 7-Dec Friday**
 * 8-Dec Saturday**
 * 9-Dec Sunday**
 * 10-DecMonday**
 * 11-DecTuesday**
 * 12-DecWednesday**
 * 13-DecThursday**
 * 14-DecFriday**
 * 15-DecSaturday**
 * 16-DecSunday**
 * 17-DecMonday**
 * 18-DecTuesday**

From Fall11

Journal Club 3 Research Report Due - Tues Oct. 11th - Guidelines in GDocs folder Virtual Screening help session for setting up your protein (should have started your second library this week)
 * Week 7 (10/10 – 10/14) **
 * Monday 4 - 5:30 in WEL 2.306 Computer Lab.

Express and Purify and Characterize your protein PyMol Image contest Due Friday at Midnight Wikispaces check on each Friday

FPLC purify enzyme, store enzyme Test your enzyme for activity Wikispaces check on each Friday
 * Week 8 (10/17 – 10/21) **
 * VIRTUAL SCREENING - should have 3 libraries completed, at the least **

Journal Club 4 FPLC purify enzyme, store enzyme Test your enzyme for activity or Sat night if working on Sat. Wikispaces check on each Friday
 * Week 9 (10/24 – 10/28) **
 * VIRTUAL SCREENING ** - finish by this week - do **Top Ligands Procotol** to get final compounds for ordering
 * Fri ( 10/28) Lab Notebook check this Friday**(7:00 pm)

=Protein Expression Scheduling:= Here's the Sign Up Form on Google - new guys and returning Spring VDSers should fill this out by Monday night.

Tues Nov. 1s t Thursday Nov 3rd at Midnight (Materials & Methods for **Cloning** due) FPLC purify enzyme, store enzyme Test your enzyme for activity Wikispaces check on each Friday
 * Week 10 (10/31 – 11/4) **
 * FRI External Open House - **Wednesday Nov. 2nd 1- 4 pm
 * Top Ligands Procotol ** to get final compounds for ordering

Journal Club 5 Inhibition Assays Tues Nov. 8th at Midnight (Materials & Methods for **Virtual Screening**due) - see Tips & Hints page Wikispaces check on each Friday
 * Week 11 (11/7 – 11/11) **

Tues Nov. 15th at Midnight (Materials & Methods for **Expression, Purification, and Characterization** due) Purify and Characterize your protein - NOTE: this is a long and arduous process. Don't put it off. Inhibition Assays or Sat night if working on Sat. Wikispaces check on each Friday
 * Week 12 (11/14 – 11/18) **
 * Fri ( 11/18) Lab Notebook check this Friday**(7:00 pm)

Protein Protocols to be completed - also see instructional videos on GDocs
ProtocolProteinExpression_VDS_Fall11_ver2.doc ProtocolProteinPurificationVDS_Fall11.doc ProtocolProteinCharacterizationVDS_Fall11.doc ProtocolFPLC_GelFiltration_VDS_Fall11V_v2.docx

ProtocolLabEnzymeTest_Phosphatase_VDS_Fall11.xlsx Then on to Inhibition Assays once compounds arrive

Journal Club 6 Inhibition Assays - on 2 compounds
 * Week 13 (11/21 – 11/25): Thanksgiving **

Inhibition Assays - on 2 compounds Intro to ICM? Tues Nov. 29th - __FINAL PRESENTATIONS of Enzyme Inhibition Graphs - everyone__ make a one slide PowerPoint and upload to the GroupMeetingSlides/FInalSlides_StudentsFall11 folder on GDocs save file with your name and date in the file name
 * Week 14 (11/28 – 12/2) - Last Class week **


 * Thurs (12/1)** - Lab Cleanup (Required) - each students spends 1 hr cleaning in lab (counts as 1 hour of lab time)
 * Fri (12/2) -** Lab Cleanup (Required) - each students spends 1 hr cleaning in lab (counts as 1 hour of lab time)

Final Wikispaces check
 * Sat ( 12/3)**
 * Final Report due (5:00 pm )**
 * Final Lab Notebook check** (7:00 pm)