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Jeanette's Research
Transform Efficiency Calculations: Sample A: 48/1ng * 1000ng/1ug = 4.8e^4 transformants/ug plasmid Sample B: 144/5ng * 1000ng/1ug = 2.88e^4 transformants/ug plasmid Sample C: 368/25ng * 1000ng/1ug = 1.472e^4 transformants/ug plasmid



Any thoughts on why the left side didn't work? - Dr. B The anneal temperature during PCR may have been too high. None of the experimental lanes appeared. Lane 8 Elution 1 has a light band of the protein, ideally it should have been darker. Lane 9 Elution 2 had nothing as expected. This gel was ran to confirm that PCR worked and oligo mix was made correctly. My first PCR had a slight smear and the second didn't. The PCR will be made again. Primary PCR had a slight smear and the secondary PCR had a band. Success.