Target+-+Probable+Adenosylhomocysteinase+SAHH+(S-Adenosyl-L-Homocysteine+Hydrolase)+(Adohcyase)+(Mycobacterium+tuberculosis)

=**Target (protein/gene name):**= =**NCBI Gene # or RefSeq#:**= 203282344 =**Protein ID (NP or XP #) or Wolbachia#:**= Rv3248c =**Organism (including strain):**= Mycobacterium Tuberculosis H37Rv =**Etiologic Risk Group (see link below):**= 3 =**Background/ Disease Information (sort of like the Intro to your Mini Research Write up):**= Mycobacterium Tuberculosis is a small, aerobic, bacterial pathogen which causes the disease Tuberculosis. Tuberculosis often affects the lungs and is spread by respiratory fluids which patients transmit through the air. This disease cannot be transmitted be shaking hands, sharing food or drink, touching bed linens or toilet seats, sharing toothbrushes, or kissing.
 * PROBABLE ADENOSYLHOMOCYSTEINASE SAHH (S- ADENOSYL-L-HOMOCYSTEINE HYDROLASE) (ADOHCYASE) **

The symptoms of this disease are chronic cough, bloody sputum, fever, night-sweats, weight loss, no appetite, and chills. This disease is very common throughout the world with more cases occurring in developing countries. Persons with HIV, who have had TB before, drug and alcohol abusers, and those with other health problems are particularly susceptible to contracting Tuberculosis.

S-Asenosyl-L-Homosysteine Hydrolase is a protein present in Mycobacterium Tuberculosis which has functions in cysteine and methionine metabolism and Selenoamino acid metabolism. These are processes which are vital to the survival of the bacterium which, when inhibited, may reduce or stop the effects of the disease Tuberculosis. =

= =**Link to TDR Targets page (if present):**=
 * []**

=**Link to Gene Database page (NCBI, EuPath databases -e.g. Try**** Tryp, PlasmoDB, etc - or PATRIC, etc.): **= []

=**Essentiality of this protein:**= Rv3248c has essentiality data Gene/Ortholog: mtu3307 (OG4_10673); Phenotype: essential; Source study: nmpdr Gene/Ortholog: sce1759 (OG4_10673); Phenotype: inviable; Source study: yeastgenome Gene/Ortholog: cel11695 (OG4_10673); Phenotype: Larval/Adult Lethal/Arrest; Source study: neb Gene/Ortholog: cel11695 (OG4_10673); Phenotype: Morphology Defect; Source study: neb Gene/Ortholog: cel11695 (OG4_10673); Phenotype: Growth Defect; Source study: neb Gene/Ortholog: cel11695 (OG4_10673); Phenotype: Embryonic Lethal/Arrest; Source study: neb Gene/Ortholog: cel11695 (OG4_10673); Phenotype: Embryonic Lethal/Arrest; Source study: wormbase Gene/Ortholog: cel11695 (OG4_10673); Phenotype: Larval/Adult Lethal/Arrest; Source study: wormbase Gene/Ortholog: Tb11.01.1350 (OG4_10673); Phenotype: significant gain of fitness in bloodstream forms (3 days); Source study: alsford Gene/Ortholog: Tb11.01.1350 (OG4_10673); Phenotype: significant gain of fitness in bloodstream forms (6 days); Source study: alsford Gene/Ortholog: Tb11.01.1350 (OG4_10673); Phenotype: no significant loss or gain of fitness in procyclic forms; Source study: alsford Gene/Ortholog: Tb11.01.1350 (OG4_10673); Phenotype: no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms; Source study: alsford =**Complex of proteins:**= ternary, with NAD and 3-deazaadenosine =**Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):**= 0.5 =** *EC#: **= 3.3.1.1 =**Link to BRENDA EC# page:**= []
 * --** Show screenshot of BRENDA enzyme mechanism schematic

=** Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**= [] Potassium Phosphate [] S-Adenosyl-L-Homocysteine Solution (SAH) [] Adenosine Deaminase Enzyme Solution (ADA) [] (This is an alternate. Original product was discontinued) Xanthine Oxidase Enzyme Solution (XO) [] Nucleoside Phosphorylase Enzyme Solution (NP) [] (Product discontinued) (see above links)
 * -- link to Sigma (or other company ) page for assay (see Sigma links below)**
 * -- links to assay reagents (substrates) pages.**
 * --- List cost and quantity of substrate reagents, supplier, and catalog #**

=**Structure Available (PDB or Homology model)**= -- PDB # or closest PDB entry if using homology model: **2ZIZ**

=**Current Inhibitors:**= [|CHEMBL405186] [|CHEMBL262063] [|NORARISTEROMYCIN] [|CHEMBL261619] [|CHEMBL129469] [|CHEMBL49935] =**Expression Information (has it been expressed in bacterial cells):**= []
 * Has been expressed in E. Coli**

=** Purification Method :**= purified as a recombinant His6-tagged protein (HTc-SahH) from //Escherichia coli//. (above link) =**Image of protein (PyMol with features delineated and shown separately):**=
 * Figure 1. 2ZIZ protein shown as cartoon colored by chain. Figure 2: 2ZIZ protein shown as sticks and colored by element with carbons as green.**

=***Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):**= code format="genbank"        1 mtgnlvtkns ltpdvrngid fkiadlslad fgrkelriae hempglmslr reyaevqplk 61 garisgslhm tvqtavliet ltalgaevrw ascnifstqd haaaavvvgp hgtpdepkgv 121 pvfawkgetl eeywwaaeqm ltwpdpdkpa nmilddggda tmlvlrgmqy ekagvvppae 181 eddpaewkvf lnllrtrfet dkdkwtkiae svkgvteett tgvlrlyqfa aagdlafpai 241 nvndsvtksk fdnkygtrhs lidginrgtd aliggkkvli cgygdvgkgc aeamkgqgar 301 vsvteidpin alqammegfd vvtveeaigd adivvtatgn kdiimlehik amkdhailgn 361 ighfdneidm aglersgatr vnvkpqvdlw tfgdtgrsii vlsegrllnl gnatghpsfv 421 msnsfanqti aqielwtknd eydnevyrlp khldekvari hvealgghlt kltkeqaeyl 481 gvdvegpykp dhyry code =**Length of your protein in Amino Acids:** <span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11px;"> 495 = =** Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website:**= code 54323.6 code =**Molar Extinction coefficient of your protein at 280 nm wavelength:**= code Ext. coefficient   67505 Abs 0.1% (=1 g/l)  1.243, assuming all pairs of Cys residues form cystines

Ext. coefficient   67380 Abs 0.1% (=1 g/l)  1.240, assuming all Cys residues are reduced code =** TMpred graph Image: **= =***CDS Gene Sequence (paste as text only):**= code <span style="background-color: #ffffff; font-family: monospace,serif;"><span class="ff_line">TCAGTAGCGGTAGTGGTCCGGCTTGTAGGGACCCTCGACGTCGACGCCGAGGTATTCGGCCTGCTCCTTG GTCAGCTTGGTCAGGTGACCGCCAAGGGCCTCGACATGGATTCGAGCCACCTTCTCGTCGAGGTGCTTGG GCAGCCGGTACACCTCGTTGTCGTACTCGTCGTTCTTGGTCCACAGCTCGATCTGGGCGATCGTCTGGTT AGCGAAGCTGTTGCTCATCACGAACGAGGGGTGCCCGGTGGCATTGCCCAGGTTCAGCAGCCGCCCCTCG GACAGCACGATGATCGAGCGGCCCGTGTCGCCAAAGGTCCACAGGTCGACCTGAGGCTTGACGTTGACCC GTGTCGCCCCGGAGCGCTCCAGCCCGGCCATGTCGATCTCGTTGTCGAAGTGGCCGATATTTCCCAGGAT CGCGTGGTCCTTCATCGCCTTAATGTGCTCGAGCATGATGATGTCTTTGTTGCCGGTCGCGGTTACGACG ATGTCGGCGTCCCCGATGGCCTCCTCGACGGTGACCACGTCGAAGCCCTCCATCATGGCCTGCAGCGCGT TGATCGGGTCGATCTCGGTGACGGAGACCCGCGCTCCCTGGCCCTTCATCGCCTCCGCACAGCCCTTACC GACGTCGCCGTAGCCGCAGATGAGGACCTTCTTACCGCCGATCAGCGCGTCGGTGCCGCGGTTGATGCCG TCGATCAGGGAGTGCCGAGTGCCGTACTTGTTGTCGAATTTGGACTTGGTCACCGAGTCGTTGACGTTGA TCGCCGGGAAGGCCAGATCCCCGGCCGCGGCGAATTGGTAGAGCCGCAGCACGCCGGTGGTGGTCTCCTC GGTGACGCCCTTGACCGACTCGGCTATCTTGGTCCACTTGTCCTTGTCGGTCTCGAAGCGGGTCCGTAGC AGGTTCAGGAAGACCTTCCACTCGGCGGGGTCGTCCTCCTCGGCGGGCGGCACCACGCCGGCCTTCTCAT ACTGCATGCCGCGCAGCACCAACATGGTGGCGTCACCGCCGTCATCGAGGATCATGTTGGCCGGCTTGTC GGGGTCCGGCCAGGTGAGCATCTGCTCGGCGGCCCACCAGTACTCTTCGAGCGTCTCGCCCTTCCACGCG AACACCGGGACACCCTTGGGCTCGTCGGGGGTGCCGTGCGGGCCGACCACGACGGCGGCGGCGGCGTGAT CCTGGGTGGAGAAGATGTTGCACGAGGCCCAGCGGACTTCGGCGCCCAGCGCGGTGAGGGTTTCGATCAA CACCGCGGTCTGCACCGTCATGTGCAGCGAACCCGAGATCCGGGCCCCCTTCAGGGGTTGCACCTCGGCA TACTCGCGCCGCAGCGACATCAGGCCGGGCATCTCGTGCTCGGCGATCCGGAGTTCTTTGCGGCCGAAAT CCGCTAGTGACAGGTCGGCGATCTTAAAGTCGATGCCGTTACGAACGTCAGGGGTCAGCGAATTTTTGGT CACCAAATTTCCGGTCAT code =**GC% Content for gene:**= Not Available =**CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):**= Not Available =**GC% Content for gene (codon optimized):**= Not Available

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**
 * ( link to DNA Works output text file - **that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences)**