Target+-+Arsenate+reductase+(S.+aureus)

==** *Target (protein/gene name): **Rv2122c, Mycobacterium tuberculosis, Probable phosphoribosyl-AMP pyrophosphatase HisE ==
 * NCBI Gene # or RefSeq#: 958674**
 * Protein ID (NP or XP #) or Wolbachia#:** MTCY182.45, MTCY398.12

====***Organism (including strain):** Mycobacterium tuberculosis ====


 * Etiologic Risk Group (see link below):** Risk Group 2 (WHO)

The // Mycobacterium tuberculosis // causes, a virulent disease that has infected a large portion of the world's population. It is spread through aerosols, so actions such as coughing or sneezing from an infected individual may release the pathogen into the air. The symptoms of this disease include chronic coughing, chest pain, fever, and chills. This is why individuals who are immunodeficient are highly susceptible to tuberculosis. Though the pathogen normally targets the lungs, other parts of the body are susceptible too.
 * Background/ Disease Information (sort of like the Intro to your Mini Research Write up):**


 * Link to TDR Targets page (if present):** @http://tdrtargets.org/targets/view?gene_id=6216

@http://www.ncbi.nlm.nih.gov/protein/CAD96999.1
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.):**


 * Essentiality of this protein:**
 * Rv2122c ** has essentiality data
 * Gene/Ortholog: ** mtu2155 (OG4_20466); **Phenotype: ** essential; **Source study: ** nmpdr

> No chemical compounds associated to this gene
 * Complex of proteins?: No**
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):**
 * **Curated from literature**
 * **Predicted associations**
 * **By orthology to known druggable targets**
 * **By sequence similarity to non orthologous druggable targets**

@http://www.brenda-enzymes.org/php/result_flat.php4?ecno=3.6.1.31&Suchword=&organism%5B%5D=Mycobacterium+tuberculosis&show_tm=0 **Assay information** No assay information for this target. **Reagent availability**
 * EC#:** 3.6.1.31
 * Link to BRENDA EC# page:**
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**

<span style="background-color: #e5ecf9; color: #333333; font-family: 'Lucida Grande','Trebuchet MS','Bitsream Vera Sans',Verdana,Helvetica,sans-serif; font-size: 11.111111640930176px;">No reagent availability information for this target.
 * -- link to Sigma (or other company ) page for assay (see Sigma links below)-** []
 * --- List cost and quantity of substrate reagents, supplier, and catalog #** Ioxynil PESTANAL- <span style="background-color: #ffffff; font-family: Arial,Helvetica,sans-serif; font-size: 11px;">36131-100MG - $45.50- Sigma-Aldrich

-- PDB # or closest PDB entry if using homology model: 1Y6X
 * Structure Available (PDB or Homology model)**

code >MQQSLAVKTFEDLFAELGDRARTRPADSTTVAALDGGVHALGKKLLEEAGEVWLAAEHESNDALAEEISQ LLYWTQVLMISRGLSLDDVYRKL code code 10274.6 kiloDaltons code code ATGACCGACGCTGAGACATCACCGCAGGGGCCGCTGGACCCCACGACTCTGGATTTCGCCAAGATGGACG GCCTGCTGCCCTGCATCGTGCAAGACGCCGACACCGCGCAGGTGCTGATGCTGGGCTATATGAACGAAGA AGCGCTTGAGCGCACGCAGGAGCGTGGCCTGGTCACCTTTTACAGCCGGTCCAAGGGGCGCCTGTGGACC AAAGGTGAAAGTTCCGGCCACACCTTGCAGCTGGTCCGCCTAAGCACCGACTGCGACAGCGACGCACTGT TGGTCCTGACCCGCCCACAGGGACCGACCTGCCACACCGGGGCCGTCAGCTGCTTTAGCGTGCAGCCGCC TGCACTGGAAATGCTGGGCCGCCTGGAACGCACTGTGCAGGGCCGCCGGGACGCCGACCCGCAGGCGAGC TACACCGCCCGGCTCCTGCAGGGCGAACCGCGCCGCGCGGCCCAGAAGGTAGGCGAGGAAGGAGTGGAAG TGGCCCTGGCCGCTCTGGCGCAAAGTGACGAGGAGTTGGCGGGTGAGGCCGCCGACCTGCTCTATCACCT GCTGGTGGTGCTGGCCCTGCGCGGCCTGCGGTTGGCAGATGTGCTGGCCGTGCTGCACGGGCGTTCCCAC TGA code
 * Current Inhibitors:** none
 * Expression Information (has it been expressed in bacterial cells):** no
 * Purification Method :** 6x HIS Ni-NTA Column Chromatography
 * Image of protein (PyMol with features delineated and shown separately):**
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):**
 * length of your protein in Amino Acids:** 93
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website:**
 * Molar Extinction coefficient of your protein at 280 nm wavelength:**
 * TMpred graph Image** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
 * CDS Gene Sequence (paste as text only):**
 * GC% Content for gene:** 58.44%

code ATGACCGACGCTGAGACATCACCGCAGGGGCCGCTGGACCCCACGACTCTGGATTTCGCCAAGATGGACG GCCTGCTGCCCTGCATCGTGCAAGACGCCGACACCGCGCAGGTGCTGATGCTGGGCTATATGAACGAAGA AGCGCTTGAGCGCACGCAGGAGCGTGGCCTGGTCACCTTTTACAGCCGGTCCAAGGGGCGCCTGTGGACC AAAGGTGAAAGTTCCGGCCACACCTTGCAGCTGGTCCGCCTAAGCACCGACTGCGACAGCGACGCACTGT TGGTCCTGACCCGCCCACAGGGACCGACCTGCCACACCGGGGCCGTCAGCTGCTTTAGCGTGCAGCCGCC TGCACTGGAAATGCTGGGCCGCCTGGAACGCACTGTGCAGGGCCGCCGGGACGCCGACCCGCAGGCGAGC TACACCGCCCGGCTCCTGCAGGGCGAACCGCGCCGCGCGGCCCAGAAGGTAGGCGAGGAAGGAGTGGAAG TGGCCCTGGCCGCTCTGGCGCAAAGTGACGAGGAGTTGGCGGGTGAGGCCGCCGACCTGCTCTATCACCT GCTGGTGGTGCTGGCCCTGCGCGGCCTGCGGTTGGCAGATGTGCTGGCCGTGCTGCACGGGCGTTCCCAC TGA code
 * CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):**


 * GC% Content for gene (codon optimized):** 45.67%

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**
 * ( link to DNA Works output text file - **that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences):**