Biofilm-associated+protein+(BAP) Acinetobacter+baumannii

http://www.bacterio.net/-hazard.html#group2
 * Target: ** Nucleoside Diphosphate Kinase
 * NCBI RefSeq#: ** NZ_CP009257.1
 * *Protein ID (NP or XP #) or Wolbachia#: **
 * Organism (including strain): ** Acinetobacter baumannii (strain SDF)
 * Etiologic Risk Group (see link below): ** Group 2

@https://www.cdc.gov/hai/organisms/pseudomonas.html
 * Disease Information (sort of like the Intro to your Mini Research Write up):** Most serious // Pseudomonas // infections occur in hospitals, in patients with already-weakened immune systems or surgical/burn wounds. If severe, as in the cases of blood infection, pneumonia, and post-surgery infections, this can cause illness and death in patients. They can be spread on the hands of healthcare workers or equipment, especially breathing machines or catheters.

@https://www.ncbi.nlm.nih.gov/gene/?term=881248 @https://www.uniprot.org/uniprot/Q03023 https://www.patricbrc.org/view/Genome/208964.12#view_tab=features&filter=keyword(aprA)
 * Link to TDR Targets page (if present): ** n/a
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) **

@http://www.jimmunol.org/content/188/1/386.long Is it a monomer or multimer as biological unit ** ? (make prediction at ** @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): multimer @https://pubs.acs.org/doi/full/10.1021/acs.jmedchem.5b01698?src=recsys
 * Essentiality of this protein: ** Alkaline metalloproteinase cleaves the complement C1q and C3, which are involved in complement-mediated phagocytosis, and prevents killing by human neutrophils.
 * Complex of proteins?: ** n/a
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **

@http://www.brenda-enzymes.org/enzyme.php?ecno=3.4.24.40
 * *EC#: ** 3.4.24.40
 * Link to BRENDA EC# page: **
 * -- ** Show screenshot of BRENDA enzyme mechanism schematic

https://www.sciencedirect.com/science/article/pii/S0022283611012812?via%3Dihub#bbb0080
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**
 * -- link to Sigma (or other company) page for assay (see Sigma links below)**
 * -- -or link (or citation) to paper that contains assay information **


 * -- links to assay reagents (substrates) pages. **
 * https://shop.bachem.com/h-7145.htm
 * https://www.sigmaaldrich.com/catalog/search?term=4%2C4%27-dithiodipyridine&interface=All&N=0&mode=match%20partialmax&lang=en&region=US&focus=produc
 * --- List cost and quantity of substrate reagents, supplier, and catalog **
 * Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly ethyl ester: Bachem, $870.00/25mg, Catalog # 4012251
 * DTDP: Sigma Aldrich, $38.80/g, Catalog # 143057

http://www.brenda-enzymes.org/enzyme.php?ecno=3.4.24.40#INHIBITORS
 * Structure (PDB or Homology model): ** 1KAP
 * Current Inhibitors: **

@http://www.jimmunol.org/content/188/1/386.long @http://www.jimmunol.org/content/188/1/386.long
 * Expression Information (has it been expressed in bacterial cells): ** Yes
 * Purification Method:** His trap column

code format="genbank" mssnslalkg rsdaytqvdn flhayarggd elvnghpsyt vdqaaeqilr eqaswqkapg dsvltlsysf ltkpndffnt pwkyvsdiys lgkfsafsaq qqaqaklslq swsdvtnihf vdagqgdqgd ltfgnfsssv ggaafaflpd vpdalkgqsw ylinssysan vnpangnygr qtltheight lglshpgdyn agegdptyad atyaedtray svmsyweeqn tgqdfkgays sapllddiaa iqklyganlt trtgdtvygf nsnterdfys atssssklvf svwdaggndt ldfsgfsqnq kinlnekals dvgglkgnvs iaagvtvena iggsgsdlli gndvanvlkg gagndilygg lgadqlwgga gadtfvygdi aessaaapdt lrdfvsgqdk idlsgldafv ngglvlqyvd afagkagqai lsydaaskag slaidfsgda hadfainlig qatqadivv** *length of your protein in Amino Acids: ** 479aa code code ATGTCCAGCAATTCTCTTGCATTGAAAGGTCGTAGCGATGCGTATACCCAGGTAGACAAC TTCCTGCATGCCTATGCGCGGGGCGGGGACGAATTGGTCAATGGCCATCCGTCCTATACC GTCGACCAGGCGGCGGAGCAGATCCTCCGCGAACAGGCGTCTTGGCAGAAAGCGCCGGGC GACTCGGTGCTGACCCTGTCCTATTCGTTCCTGACCAAACCGAACGACTTCTTCAATACG CCGTGGAAGTATGTCAGCGATATCTACTCGCTGGGCAAGTTCAGCGCCTTTTCCGCGCAG CAGCAGGCCCAGGCCAAGTTGTCGCTGCAATCCTGGTCGGACGTCACCAATATCCACTTC GTCGACGCCGGCCAGGGCGATCAGGGCGACCTGACCTTCGGCAACTTCAGCAGTAGTGTC GGCGGTGCGGCGTTCGCCTTCCTGCCGGATGTACCGGATGCGCTCAAGGGGCAATCCTGG TACCTGATCAACAGCAGCTACAGCGCCAACGTCAATCCGGCCAACGGCAACTACGGACGC CAGACCCTGACCCACGAGATCGGCCATACCCTGGGCCTGAGCCACCCCGGCGACTACAAC GCCGGCGAGGGCGATCCCACCTACGCCGACGCTACCTACGCCGAGGACACCCGCGCCTAT TCGGTGATGAGCTACTGGGAAGAGCAGAACACCGGCCAGGACTTCAAGGGCGCCTATTCC TCGGCACCGCTGCTGGACGACATCGCGGCGATCCAGAAGCTCTACGGGGCCAACCTGACC ACTCGCACCGGCGACACGGTGTACGGCTTCAACTCCAACACCGAGCGCGACTTCTACAGC GCCACCTCGTCCAGTTCCAAGCTGGTGTTCTCGGTGTGGGACGCCGGCGGCAACGACACC CTGGACTTCTCCGGCTTCAGCCAGAACCAGAAGATCAACCTCAACGAGAAGGCGCTGTCC GATGTCGGCGGGTTGAAGGGCAATGTGTCGATCGCTGCCGGGGTCACCGTGGAAAACGCC ATCGGCGGCTCGGGTAGCGACCTGTTGATCGGCAACGACGTGGCCAACGTGCTCAAGGGC GGCGCCGGCAACGACATCCTCTACGGCGGCCTCGGCGCGGACCAGCTGTGGGGTGGCGCG GGAGCCGACACCTTCGTCTACGGCGATATCGCCGAGTCCTCCGCGGCGGCGCCGGATACC CTGCGCGACTTCGTCAGCGGCCAGGACAAGATCGACCTGTCCGGGCTGGACGCCTTCGTC AACGGCGGGCTGGTGCTGCAATACGTCGACGCCTTCGCCGGCAAGGCCGGCCAGGCGATC CTGTCCTACGACGCGGCGAGCAAGGCCGGCAGCCTGGCGATCGACTTCAGCGGGGACGCC CATGCCGATTTCGCGATCAATCTGATCGGCCAGGCGACCCAGGCCGACATCGTCGTCTGA ** *GC% Content for gene: ** 63.61% code
 * Image of protein (PyMol with features delineated and shown separately): **
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website:** 50432.96 kDa
 * Molar Extinction coefficient of your protein at 280 nm wavelength:** 71280
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
 * CDS Gene Sequence (paste as text only):**