Target-+Inositol-phosphate+phosphatase+(S.+aureus)................................Brandon+N.

***NCBI Gene # or RefSeq#:** 5777523 ***Protein ID (NP or XP #) or Wolbachia#:** YP_001576165.1 ***Organism:** Staphylococcus aureus ***Background/Disease Information:** S. aureus is a facultatively anaerobic, Gram-positive coccus, which appears as grape-like clusters when viewed through a microscope, and has large, round, golden-yellow colonies, often with hemolysis, when grown on blood agar plates.S. aureus can cause a range of illnesses, from minor skin infections, such as pimples, impetigo, boils (furuncles), cellulitis folliculitis, carbuncles, scalded skin syndrome, and abscesses, to life-threatening diseases suchas pneumonia, meningitis, osteomyelitis, endocarditis, toxic shock syndrome (TSS),bacteremia, and sepsis. Its incidence ranges from skin, soft tissue, respiratory, bone, joint, endovascular to wound infections. It is still one of the five most common causes of nosocomial infections and is often the cause of postsurgical wound infections. Each year, some 500,000 patients in American hospitals contract a staphylococcal infection.
 * Target (protein/gene name):** Inositol-phosphate phosphatase
 * Etiologic Risk Group (see link below): ** Risk Group 2 (RG2)

**Essentiality of this protein:** None according to TDR **Complex of enzymes:** None ***EC#:** 3.1.3.25 **Link to BRENDA EC# page:** [] -- Show screenshot of BRENDA enzyme mechanism schematic



[] -- link to Sigma (or other company) page for assay or assay reagents (substrates) -- link to paper that contains assay information -- List cost and quantity of substrate reagents and supplier
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **

-- PDB # or closest PDB entry if using homology model: 3T0J -- Query Coverage (if not direct match): N/A -- Max Ident (if not direct match): N/A
 * Structure Available (PDB or Homology model) **

**Druggable Target (see Databases for this):** Yes

**Current Inhibitors:** None **Expression Information (has it been expressed in bacterial cells):** Yes, expressed in E. Coli **Purification Method:** []
 * Image of protein (PyMol or etc): **



MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVTNVDKQIQQQFQQFLATHFPEHQLLAE EKSNEMITNEINHLWIMDPIDGTANLVKQQEDYCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCN GIKMEEPPSLKLEDAIISFNAQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDI AAQFLFAELLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR
 * Amino Acid Sequence:**


 * length of your protein in Amino Acids:** 265 AA
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website:** 30385.0 Daltons

ATGACAGATAAAACATTACAGCAAATAGATAAGTTAATATGTTCGTGGTTGAAACAAATAGACAATGTTA TTCCGCAATTAATTATGGAAATGACTACAGAAACTAAGCGTCATCGATTTGATTTAGTTACAAATGTAGA TAAACAGATTCAACAACAATTCCAACAATTTTTAGCAACACATTTTCCGGAACACCAACTATTAGCGGAA GAAAAAAGTAATGAAATGATTACGAATGAAATTAATCATTTATGGATTATGGATCCCATTGATGGAACTG CCAATTTAGTAAAACAGCAAGAAGATTATTGTATTATATTGGCGTATTTTTATGAAGGTAAACCAATGTT ATCGTACGTATATGATTATCCGCATAAAAAGCTTTATAAAGCAATACGAGGAGAAGGTGCTTTTTGTAAT GGAATTAAGATGGAAGAACCACCATCGTTAAAATTGGAAGACGCTATTATATCGTTTAATGCACAAGTGA TGAATCTAGATACGGTGCAAGATTTATTTGACGCCTCATTTAGTTATCGTTTAGTTGGTGCTTGCGGATT AGATTCTATGCGAGTGGCAAAAGGTCAATTTGGGGCACATATTAATACAAATCCTAAACCTTGGGACATT GCTGCACAGTTTTTATTTGCAGAACTATTGAATCTAAAAATGACGACTTTAGATGGTAAGGCAATTGATC ATTTAAAGGGTGCACCTTTTATTATAAGTAATAAAGCATGTCATGAAACGGTACTTAAAATTTTAAATGC AAATGGTGGTTATCAAAAATACAGATAG
 * CDS Gene Sequence:**

ATGACCGACAAAACCCTGCAGCAGATCGATAAACTCATTTGTTCTTGGCTCAAGCAAATC GACAATGTGATCCCTCAACTGATTATGGAAATGACGACCGAAACGAAGCGTCACCGTTTC GACCTCGTTACCAATGTTGACAAACAGATCCAACAGCAGTTCCAGCAATTCCTCGCGACC CACTTTCCTGAGCACCAGCTGCTGGCGGAAGAAAAATCTAATGAAATGATTACCAACGAA ATCAACCACCTCTGGATCATGGACCCGATCGACGGTACGGCGAACCTGGTTAAACAACAG GAAGACTACTGCATCATCCTGGCGTACTTCTACGAAGGTAAACCTATGCTGTCTTACGTT TATGACTATCCTCACAAAAAACTGTATAAAGCTATCCGCGGTGAGGGTGCATTTTGCAAC GGTATCAAAATGGAAGAACCGCCTTCTCTCAAACTGGAAGACGCCATCATCTCTTTTAAC GCGCAGGTTATGAACCTCGACACTGTTCAGGACCTGTTCGATGCGTCCTTTTCTTATCGC CTGGTTGGTGCGTGCGGTCTGGACTCTATGCGTGTTGCTAAAGGTCAGTTCGGTGCCCAC ATCAACACCAATCCTAAGCCTTGGGACATCGCAGCGCAATTTCTCTTCGCGGAACTCCTC AATCTCAAGATGACCACGCTGGACGGTAAAGCCATCGATCACCTGAAAGGCGCTCCGTTC ATTATTTCTAACAAAGCGTGCCACGAAACCGTTCTGAAAATTCTCAATGCGAACGGTGGT TACCAGAAATACCGCCTGGGTGACTCTCCGGGTTACGTTATGTCTAACATCGAATTCCGT CAGCTCACCCGTGGCCATTCTCCATCTGACGAACGTGAAGCTCGCCGTGTCGAAGAAGCC GGTGGTCAGCTGTTCGTTATTGGTGGTGAACTCCGTGTAAATGGTGTGCTGAACCTGACG CGTGCCCTGGGCGACGTTCCGGGTCGTCCGATGATCAGCAACGAACCGGAAACCTGTCAA GTTCCGATTGAGAGCAGCGACTATCTGGTACTCCTGGCATGCGACGGCATCTCTGATGTG TTCAACGAGCGTGATCTGTACCAACTGGTTGAAGCCTTCGCAAACGACTACCCGGTTGAG GATTATGCAGAACTGTCTCGTTTCATCTGCACCAAGGCGATCGAGGCTGGTTCTGCGGAC AACGTCTCTGTTGTGATCGGTTTCCTGCGTCCGCCGCAAGACGTTTGGAAACTGATGAAG CACGAGTCTGATGACGAGGACTCTGACGTTACCGACGAAGAATAA
 * GC% Content for gene:** 32%
 * CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol:**
 * GC% Content for gene (codon optimized):** 50%

1 ATGACCGACAAAACCCTGCAGCAGATCGATAAACTCATTTGTTCTTGGCTCAAGC 55 2 TCATTTCCATAATCAGTTGAGGGATCACATTGTCGATTTGCTTGAGCCAAGAACAAATGA 60 3 CCCTCAACTGATTATGGAAATGACGACCGAAACGAAGCGTCACCGTTTCGACCTCGTTAC 60 4 GGAATTGCTGGAACTGCTGTTGGATCTGTTTGTCAACATTGGTAACGAGGTCGAAACGGT 60 5 ACAGCAGTTCCAGCAATTCCTCGCGACCCACTTTCCTGAGCACCAGCTGCTGGCGGAAGA 60 6 TCCAGAGGTGGTTGATTTCGTTGGTAATCATTTCATTAGATTTTTCTTCCGCCAGCAGCT 60 7 CGAAATCAACCACCTCTGGATCATGGACCCGATCGACGGTACGGCGAACCTGGTTAAACA 60 8 CTTCGTAGAAGTACGCCAGGATGATGCAGTAGTCTTCCTGTTGTTTAACCAGGTTCGCCG 60 9 CCTGGCGTACTTCTACGAAGGTAAACCTATGCTGTCTTACGTTTATGACTATCCTCACAA 60 10 ATGCACCCTCACCGCGGATAGCTTTATACAGTTTTTTGTGAGGATAGTCATAAACGTAAG 60 11 CGCGGTGAGGGTGCATTTTGCAACGGTATCAAAATGGAAGAACCGCCTTCTCTCAAACTG 60 12 GGTTCATAACCTGCGCGTTAAAAGAGATGATGGCGTCTTCCAGTTTGAGAGAAGGCGGTT 60 13 ACGCGCAGGTTATGAACCTCGACACTGTTCAGGACCTGTTCGATGCGTCCTTTTCTTATC 60 14 AACACGCATAGAGTCCAGACCGCACGCACCAACCAGGCGATAAGAAAAGGACGCATCGAA 60 15 GTCTGGACTCTATGCGTGTTGCTAAAGGTCAGTTCGGTGCCCACATCAACACCAATCCTA 60 16 GTTCCGCGAAGAGAAATTGCGCTGCGATGTCCCAAGGCTTAGGATTGGTGTTGATGTGGG 60 17 GCAATTTCTCTTCGCGGAACTCCTCAATCTCAAGATGACCACGCTGGACGGTAAAGCCAT 60 18 GCTTTGTTAGAAATAATGAACGGAGCGCCTTTCAGGTGATCGATGGCTTTACCGTCCAGC 60 19 CTCCGTTCATTATTTCTAACAAAGCGTGCCACGAAACCGTTCTGAAAATTCTCAATGCGA 60 20 GGAGAGTCACCCAGGCGGTATTTCTGGTAACCACCGTTCGCATTGAGAATTTTCAGAACG 60 21 CGCCTGGGTGACTCTCCGGGTTACGTTATGTCTAACATCGAATTCCGTCAGCTCACCCGT 60 22 TCTTCGACACGGCGAGCTTCACGTTCGTCAGATGGAGAATGGCCACGGGTGAGCTGACGG 60 23 GCTCGCCGTGTCGAAGAAGCCGGTGGTCAGCTGTTCGTTATTGGTGGTGAACTCCGTGTA 60 24 GGAACGTCGCCCAGGGCACGCGTCAGGTTCAGCACACCATTTACACGGAGTTCACCACCA 60 25 CCCTGGGCGACGTTCCGGGTCGTCCGATGATCAGCAACGAACCGGAAACCTGTCAAGTTC 60 26 TCGCATGCCAGGAGTACCAGATAGTCGCTGCTCTCAATCGGAACTTGACAGGTTTCCGGT 60 27 GGTACTCCTGGCATGCGACGGCATCTCTGATGTGTTCAACGAGCGTGATCTGTACCAACT 60 28 ATAATCCTCAACCGGGTAGTCGTTTGCGAAGGCTTCAACCAGTTGGTACAGATCACGCTC 60 29 GACTACCCGGTTGAGGATTATGCAGAACTGTCTCGTTTCATCTGCACCAAGGCGATCGAG 60 30 GCAGGAAACCGATCACAACAGAGACGTTGTCCGCAGAACCAGCCTCGATCGCCTTGGTGC 60 31 TGTTGTGATCGGTTTCCTGCGTCCGCCGCAAGACGTTTGGAAACTGATGAAGCACGAGTC 60 32 TTATTCTTCGTCGGTAACGTCAGAGTCCTCGTCATCAGACTCGTGCTTCATCAGTTTC 58
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **

@https://docs.google.com/file/d/0B4O2KqKh2q_-cWEzcmlETE9MN2s/edit


 * Primer design results for 'tail' primers (this is just 2 sequences): **

http://www.ncbi.nlm.nih.gov/pubmed/22360485 @http://enzyme.expasy.org/EC/3.1.3.25 @http://www.brenda-enzymes.org/php/result_flat.php4?ecno=3.1.3.25&Suchword=&organism%5B%5D=Staphylococcus+aureus&show_tm=0 @http://www.brenda-enzymes.org/literature/lit.php4?e=3.1.3.25&r=713666
 * Literature:**