Dengue+Methyltransferase

Target Name: Dengue Methyltranferase E.C. Number: 2.1.1.57 PDB ID: 1L9K Organism: Dengue Virus type 2 (strain Puerto Rico) Ethiological Risk Group: n/a

Disease Information: Dengue Fever is a mosquito-born virus that is a member of the Flaviviride virus family. The virus can result in symptoms such as fever, chills, nausea, rashes, and joint pain. It is most common in areas with a high density population and is only spread by infected mosquitos. It is also one of the most common arthropod born diseases.

[|Dengue Overview] [|embopress.org]

Essentiallity: The target is essential for the synthesis of RNA and invest themselves in the replication machinery of the virus.

[|Essentiality]

[|Brenda]

Link [|ExPasy]

Structure:



TMPred page: [|Expasy TMpred]

Gene Sequence: >fig|243365.4.peg.112|CV_0121|VBIChrVio67196_0112| SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase (EC 2.1.1.182) [Chromobacterium violaceum ATCC 12472 | 243365.4] atgagcaagcacatcccgcgcaagcgtttcgggcagaacttcctgcaggacgccagcgtc atcgccggcatcgtccacgcggtcaatccgcagcctgacgacatcgtcatcgagatcggc cccgggctgggcgccatcaccaagccgttgctggcgcgcctcaagcatctgcacgtggtg gagatcgaccgcgacatcatcgagcggctcaaggccgaacatccggccgacaagctgacc atccacgccggcgacgcgctggccttcgacttcgcctcggtcagcgaggcgccgctgaag atcgtcggcaacctgccgtacaatatctccacgccgctgctgttccacctggccagctac ggcaaccgggtgacggacatgcatttcatgctgcagaaggaagtgatagagcgcatggtg gccgagccgtccaccgccgattacggccggctgacagtgatgctgcagtaccgtttctac atggaaaacatcctgttcgtgccgccggaggcgttctggccgccgccgaaggtggattcc gccgtggtgcggatgattccggcgccgggccgctgcggcaccgcgcgcgatgaggcgctg ctggagaagctggtgagccaggcgttcgcccagcgccgcaagacgctgcgcaacaacctg aagggcctggccgacgcggccgacctggaggcgctgggcatcgaccccggcctgcgtccg gaaaacctgccggtggaggatttcgtccgcctggccaaccatctgcacgacaagggcgcg cgcggctga

Protein Sequence:

<span style="background-color: transparent; color: #333333; font-family: monospace; font-size: 14.2px; text-decoration: none;">>fig|243365.4.peg.112|CV_0121|VBIChrVio67196_0112| SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase (EC 2.1.1.182) [Chromobacterium violaceum ATCC 12472 | 243365.4]

<span style="background-color: transparent; color: #333333; font-family: monospace; font-size: 14.2px; text-decoration: none;">MSKHIPRKRFGQNFLQDASVIAGIVHAVNPQPDDIVIEIGPGLGAITKPLLARLKHLHVV

<span style="background-color: transparent; color: #333333; font-family: monospace; font-size: 14.2px; text-decoration: none;">EIDRDIIERLKAEHPADKLTIHAGDALAFDFASVSEAPLKIVGNLPYNISTPLLFHLASY

<span style="background-color: transparent; color: #333333; font-family: monospace; font-size: 14.2px; text-decoration: none;">GNRVTDMHFMLQKEVIERMVAEPSTADYGRLTVMLQYRFYMENILFVPPEAFWPPPKVDS

<span style="background-color: transparent; color: #333333; font-family: monospace; font-size: 14.2px; text-decoration: none;">AVVRMIPAPGRCGTARDEALLEKLVSQAFAQRRKTLRNNLKGLADAADLEALGIDPGLRP

<span style="background-color: transparent; color: #333333; font-family: monospace; font-size: 14.2px; text-decoration: none;">ENLPVEDFVRLANHLHDKGARG

<span style="background-color: #ffff00; color: #000000; font-family: arial,helvetica,sans-serif; font-size: 13px; text-decoration: none;">Do Not Need this info for <span class="r7mb967" style="background-color: #ffff00; color: #000000; font-family: arial,helvetica,sans-serif; font-size: 13px; text-decoration: none;">Spring <span style="background-color: #ffff00; color: #000000; font-family: arial,helvetica,sans-serif; font-size: 13px; text-decoration: none;"> (but still copy these lines to your Target page for now) <span style="background-color: transparent; color: #000000; display: block; font-family: arial,helvetica,sans-serif; font-size: 13px; text-align: left; text-decoration: none;"> Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): <span style="background-color: transparent; color: #000000; display: block; font-family: arial,helvetica,sans-serif; font-size: 13px; text-align: left; text-decoration: none;"> ( link to DNA Works output text file -  <span style="background-color: #ffffff; color: #000000; font-family: arial,helvetica,sans-serif; font-size: 13px; text-decoration: none;">that should be saved in your <span class="f1198g38" style="background-color: #ffffff; color: #000000; font-family: arial,helvetica,sans-serif; font-size: 13px; text-decoration: none;">Google Docs <span style="background-color: #ffffff; color: #000000; font-family: arial,helvetica,sans-serif; font-size: 13px; text-decoration: none;"> folder after you did the primer design protocol) <span style="background-color: #ffffff; color: #000000; font-family: arial,helvetica,sans-serif; font-size: 13px; text-decoration: none;">-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if <span class="m9l40l772" style="background-color: #ffffff; color: #000000; font-family: arial,helvetica,sans-serif; font-size: 13px; text-decoration: none;">you are not <span style="background-color: #ffffff; color: #000000; font-family: arial,helvetica,sans-serif; font-size: 13px; text-decoration: none;"> sure how to.