Bifunctional N-acetylglucosamine-1-phosphate+uridyl+transferase+(GlmU)

**Target (protein/gene name):** Bifunctional N-acetylglucosamine-1-phosphate uridyl transferase (GlmU)


 * NCBI # : **886069


 * Protein ID: **NP_215534


 * Organism: **Mycobacterium Tuberculosis

**Etiologic Risk Group (see link below):** Group 3

http://www.bacterio.net/-hazard.html#group3

Tuberculosis (TB) is caused by a bacterium called Mycobacterium tuberculosis. The bacteria usually attacks the lungs, but can also attack any part of the body such as the kidney, spine, and brain. Tuberculosis can be spread through the air, just like a cold or the flu. It is contagious, but not easy to catch. Additionally, not everyone infected with TB bacteria becomes sick. As a result, two TB-related conditions exist: latent TB infection (LTBI) and TB disease. If not treated properly, TB disease can be fatal.
 * Disease Information: **

https://www.cdc.gov/tb/topic/basics/default.htm

https://www.webmd.com/lung/understanding-tuberculosis-basics#1

http://tdrtargets.org/targets/view?gene_id=6147
 * Link to TDR targets page: **

https://www.ncbi.nlm.nih.gov/gene/886069 <span style="font-family: Times New Roman,Times,serif;">https://www.uniprot.org/uniprot/P9WMN3
 * <span style="font-family: Times New Roman,Times,serif;">Link to Gene Database page (NCBI): **

<span style="font-family: Times New Roman,Times,serif;">The //glmU// gene is essential in //Mycobacterium tuberculosis//, being required for optimal bacterial growth, and has been selected as a possible drug target for structural and functional investigation. GlmU is a bifunctional acetyltransferase/uridyltransferase that catalyses the formation of UDP-GlcNAc from GlcN-1-P. UDP-GlcNAc is a substrate for two important biosynthetic pathways: lipopolysaccharide and peptidoglycan synthesis.
 * <span style="font-family: Times New Roman,Times,serif;">Essentiality: **

<span style="font-family: Times New Roman,Times,serif;">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2651758/

<span style="font-family: Times New Roman,Times,serif;">UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) is an essential precursor of peptidoglycan and the rhamnose-GlcNAc linker region of mycobacterial cell wall. In Mycobacterium tuberculosis H37Rv genome, Rv1018c shows strong homology to the GlmU protein involved in the formation of UDP-GlcNAc from other bacteria. GlmU is a bifunctional enzyme that catalyzes two sequential steps in UDP-GlcNAc biosynthesis. Glucosamine-1-phosphate acetyl transferase catalyzes the formation of N-acetylglucosamine-1-phosphate, and N-acetylglucosamine-1-phosphate uridylyltransferase catalyzes the formation of UDP-GlcNAc. Since inhibition of peptidoglycan synthesis often results in cell lysis, M. tuberculosis GlmU is a potential anti-tuberculosis (TB) drug target.

<span style="font-family: Times New Roman,Times,serif;">https://www.ncbi.nlm.nih.gov/pubmed/18573680/


 * <span style="font-family: Times New Roman,Times,serif;">Is it a monomer or multimer as a biological unit? make prediction ** <span style="font-family: Times New Roman,Times,serif;">**at** @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html**):** multimer


 * <span style="font-family: Times New Roman,Times,serif;">Druggable Target (list number or cite evidence from a paper/database showing druggability in another organism): **<span style="font-family: Times New Roman,Times,serif;">0.2

<span style="font-family: Times New Roman,Times,serif;">http://tdrtargets.org/targets/view?gene_id=6147 <span style="font-family: Times New Roman,Times,serif;">https://www.sciencedirect.com/science/article/pii/S1472979215300159?via%3Dihub <span style="font-family: Times New Roman,Times,serif;">https://www.ncbi.nlm.nih.gov/pubmed/18573680/ <span style="font-family: Times New Roman,Times,serif;">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695056/#R84

<span style="font-family: Times New Roman,Times,serif;">**E.C# :** 2.3.1.157

<span style="font-family: Times New Roman,Times,serif;">http://www.brenda-enzymes.org/enzyme.php?ecno=2.3.1.157&Suchword=2.3.1.157&reference=&UniProtAcc=&organism%5B%5D=Mycobacterium+tuberculosis&show_tm=0
 * <span style="font-family: Times New Roman,Times,serif;">Link to BRENDA EC# page: **

<span style="font-family: Times New Roman,Times,serif;">

<span style="font-family: Times New Roman,Times,serif;">https://www.ncbi.nlm.nih.gov/pubmed/18573680 <span style="font-family: Times New Roman,Times,serif;">http://aac.asm.org/content/53/6/2306.long
 * <span style="font-family: Times New Roman,Times,serif;">Enzyme Assay Information: **


 * <span style="font-family: Times New Roman,Times,serif;">Links to assay reagents (substrates) pages: **

<span style="font-family: Times New Roman,Times,serif;">http://www.profoldin.com/glmu-assay.html

<span style="font-family: Times New Roman,Times,serif;">E.coli GlmU Assay Kit Plus-100 <span style="font-family: Times New Roman,Times,serif;">Catalong No. GLU100KE <span style="font-family: Times New Roman,Times,serif;">Price: $395.00

<span style="font-family: Times New Roman,Times,serif;">2QKX <span style="font-family: Times New Roman,Times,serif;">3D8V <span style="font-family: Times New Roman,Times,serif;">3D98 <span style="font-family: Times New Roman,Times,serif;">3DJ4 <span style="font-family: Times New Roman,Times,serif;">3FOQ <span style="font-family: Times New Roman,Times,serif;">3ST8 <span style="font-family: Times New Roman,Times,serif;">4G3Q <span style="font-family: Times New Roman,Times,serif;">4G3S <span style="font-family: Times New Roman,Times,serif;">4G87 <span style="font-family: Times New Roman,Times,serif;">4HCQ
 * <span style="font-family: Times New Roman,Times,serif;">Structure (PDB or Homology model): **

<span style="font-family: Times New Roman,Times,serif;">https://www.brenda-enzymes.org/all_enzymes.php?ecno=2.3.1.157&table=Inhibitors#TAB <span style="font-family: Times New Roman,Times,serif;">**Expression Information (has it been expressed in bacterial cells):** Yes <span style="font-family: Times New Roman,Times,serif;">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2531282/
 * <span style="font-family: Times New Roman,Times,serif;">Current Inhibitors: **

<span style="font-family: Times New Roman,Times,serif;">https://www.researchgate.net/publication/23232128_Expression_purification_and_preliminary_crystallographic_analysis_of_N_-acetylglucosamine-1-phosphate_uridylyltransferase_from_Mycobacterium_tuberculosis
 * <span style="font-family: Times New Roman,Times,serif;">Purification Method: **<span style="font-family: Times New Roman,Times,serif;">Hanging-drop vapour-diffusion method


 * <span style="font-family: Times New Roman,Times,serif;">Image of protein (PyMol with features delineated and shown separately): **

<span style="font-family: Times New Roman,Times,serif;">Amino Acid Sequence: code MTFPGDTAVL VLAAGPGTRM RSDTPKVLHT LAGRSMLSHV LHAIAKLAPQ RLIVVLGHDH QRIAPLVGEL ADTLGRTIDV ALQDRPLGTG HAVLCGLSAL PDDYAGNVVV TSGDTPLLDA DTLADLIATH RAVSAAVTVL TTTLDDPFGY GRILRTQDHE VMAIVEQTDA TPSQREIREV NAGVYAFDIA ALRSALSRLS SNNAQQELYL TDVIAILRSD GQTVHASHVD DSALVAGVNN RVQLAELASE LNRRVVAAHQ LAGVTVVDPA TTWIDVDVTI GRDTVIHPGT QLLGRTQIGG RCVVGPDTTL TDVAVGDGAS VVRTHGSSSS IGDGAAVGPF TYLRPGTALG ADGKLGAFVE VKNSTIGTGT KVPHLTYVGD ADIGEYSNIG ASSVFVNYDG TSKRRTTVGS HVRTGSDTMF VAPVTIGDGA YTGAGTVVRE DVPPGALAVS AGPQRNIENW VQRKRPGSPA AQASKRASEM ACQQPTQPPD ADQTP code <span style="font-family: Times New Roman,Times,serif;">**Length of your protein in Amino Acids:** 495 AA

<span style="font-family: Times New Roman,Times,serif;">Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website: 51583.99 kDa

<span style="font-family: Times New Roman,Times,serif;">Molar Extinction coefficient of your protein at 280 nm wavelength: 24535

<span style="font-family: Times New Roman,Times,serif;">TMpred graph Image(@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.

code >>NC_000962.3:c1138060-1136573 Mycobacterium tuberculosis H37Rv, complete genome ATGACGTTTCCTGGTGACACCGCGGTCCTGGTCCTAGCGGCCGGGCCCGGGACCCGGATGCGTTCGGACA CCCCCAAGGTGCTGCACACACTCGCCGGTCGCAGCATGCTGTCGCATGTACTGCATGCGATCGCCAAGCT GGCGCCGCAGCGTCTAATCGTGGTGCTGGGACACGATCACCAGCGCATCGCGCCGCTAGTCGGTGAACTC GCCGACACCCTAGGCCGAACGATCGACGTCGCCCTGCAGGATCGACCGCTAGGGACCGGGCATGCGGTAC TCTGCGGGCTGTCCGCGCTGCCCGATGACTACGCCGGCAACGTCGTGGTCACCTCGGGAGATACCCCGCT GCTGGACGCCGACACGCTGGCCGACTTGATCGCCACCCACCGCGCGGTGTCGGCTGCGGTGACGGTGCTG ACCACGACGCTGGATGATCCCTTCGGCTACGGCCGCATCCTGCGCACCCAGGATCACGAAGTCATGGCGA TCGTGGAGCAAACCGACGCGACACCATCGCAGCGGGAAATCCGCGAAGTCAACGCCGGCGTCTACGCCTT CGACATCGCCGCGCTGCGGTCCGCACTGAGCCGGCTGAGCTCCAACAACGCCCAACAGGAGCTCTACCTC ACCGACGTCATCGCCATCTTGCGCTCCGACGGCCAGACCGTACATGCCAGCCACGTCGACGACAGCGCGT TGGTGGCCGGCGTCAACAATCGCGTCCAGCTGGCCGAGCTGGCCTCCGAACTCAACCGGCGGGTGGTGGC CGCTCACCAGCTGGCCGGCGTCACCGTCGTCGACCCGGCTACCACCTGGATCGACGTCGACGTCACCATC GGCCGCGACACCGTCATTCACCCGGGTACCCAGTTGCTGGGCCGCACCCAGATCGGCGGTCGCTGTGTCG TCGGTCCCGACACCACCCTGACCGACGTCGCCGTCGGCGACGGTGCCTCGGTGGTGCGCACCCACGGTTC GTCGTCGTCGATTGGGGATGGCGCCGCGGTCGGGCCCTTCACCTACCTGCGGCCCGGAACCGCGTTGGGC GCCGACGGCAAGCTGGGCGCGTTCGTCGAGGTCAAGAACTCCACCATCGGCACCGGCACCAAGGTGCCGC ACCTGACCTACGTCGGCGACGCCGACATCGGCGAGTACAGCAACATCGGCGCCTCCAGCGTGTTCGTCAA CTACGACGGTACGTCCAAACGGCGCACCACCGTCGGTTCGCACGTACGGACCGGGTCCGACACCATGTTC GTGGCCCCAGTAACCATCGGCGACGGCGCGTATACCGGGGCCGGCACAGTGGTGCGGGAGGATGTCCCGC CGGGGGCGCTGGCAGTGTCGGCGGGTCCGCAACGCAACATCGAGAACTGGGTGCAGCGCAAACGCCCCGG CAGCCCAGCGGCTCAGGCCTCAAAAAGAGCCTCAGAAATGGCCTGCCAACAGCCCACACAACCACCCGAC GCTGATCAGACACCGTGA code <span style="font-family: "Times New Roman",Times,serif;">**GC Content:** 67.47%
 * <span style="font-family: "Times New Roman",Times,serif;">CDS Gene Sequence: **