Trypanosoma+brucei+protein+tyrosine+phosphatase,+putative

Trypanosoma brucei, protein tyrosine phosphatase
 * Target (protein/gene name): **

295789515 http://www.ncbi.nlm.nih.gov/protein/3M4U_A
 * NCBI Gene # or RefSeq#: **

Tb927.10.6690
 * Protein ID (NP or XP #) or Wolbachia#: **

Trypanosoma brucei
 * Organism (including strain): **

Risk Group 2
 * Etiologic Risk Group (see link below): **

//Trypanosoma brucei// are microscopic parasites that causes African Trypanosomiasis, also known as "sleeping sickness." The parasite makes its way into the blood-brain barrier and infects the central nervous system causing fever, headache, muscle and joint aches, enlarged lymph nodes and sores around the wound. As time progresses, it may cause mental deterioration and other neurologic problems. Death ensues usually within a few months. It is transmitted by the tsetse fly (//Glossina// species), which is found only in rural Africa. Although the infection is not found in the United States, it is a serious public health problem in some regions of sub-Saharan Africa. Sleeping sickness is curable with medication, but if left untreated, the infection will eventually lead to coma and death.
 * Background/Disease Information: **

[]
 * Link to TDR Targets page: **

**Link to Gene Database page:** []

Tb927.10.6690 has essentiality data
 * Essentiality of this protein: **
 * Gene/Ortholog:** Tb927.10.6690 (OG4_23831); **Phenotype:** no significant loss or gain of fitness in bloodstream forms (3 days); **Source study:** alsford
 * Gene/Ortholog:** Tb927.10.6690 (OG4_23831); **Phenotype:** no significant loss or gain of fitness in bloodstream forms (6 days); **Source study:** alsford
 * Gene/Ortholog:** Tb927.10.6690 (OG4_23831); **Phenotype:** no significant loss or gain of fitness in procyclic forms; **Source study:** alsford
 * Gene/Ortholog:** Tb927.10.6690 (OG4_23831); **Phenotype:** no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms; **Source study:** alsford

None
 * Complex of proteins: **

Druggability index (range: 0 to 1): 0.3 No chemical compounds associated to this gene No orthologous druggable targets No additional associated druggable targets
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **

3.1.3.48
 * EC#: **

[]
 * Link to BRENDA EC# page: **



http://www.brenda-enzymes.info/literature/lit.php4?e=3.1.3.48&r=95003 http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1995.871_a.x/pdf
 * Enzyme Assay Information Link: **


 * List cost and quantity of substrate reagents, supplier, and catalog #: **
 * **Substrate** || **Cost** || **Quantity** || **Supplier** || **Catalog #** ||
 * 4-nitrophenyl phosphate || 209.50 || 50 || Sigma-Aldrich Corporation || 333338-18-4 ||
 * Raytide peptide || Contact || Contact || Manufactured by EMD Biosciences, Inc || PK02 ||

3M4U
 * Structure Available (PDB or Homology model): **

H3VO4
 * Current Inhibitors: **

Escherichiu coli
 * Expression Information (has it been expressed in bacterial cells): **

Purification of plasma membranes described by Seyfang (Scyfdng and Duszenko, 1993) and fractions were stored at -80°C. Briefly. the ghost fraction (F2) was stripped of peripheral and cytoskeletal proteins by EDTA/alkali treatment (F3), and octylthioglucoside was used to solubilize plasma-membrane proteins (F4). The protein concentration was determined by the method of Bradford (1976) using BSA as the standard and 0.1% Triton X-100 to solubilize membrane proteins.
 * Purification Method: **

**Image of protein:**

MSTAKSFPMAQLSTRAQYSRMQREFVQLQRQENPRNINFTTSLKNRHKNRYLDILANEET IYPPVLKAVGAQPGRYPYINGNLIDLDLPHTFVACQAPVPQGVPDFLETLSEKKVDLVVM LTKLREGGVLKAERYWPEEEEDSLSFPESGHDAIKVTRDAEASYEVDAELDIVRRPLVIH VPGKPMHRVLQVQYVGWPDHGVPESAASFDELLSVIKNCVTTSPILVHCSAGIGRTGTLI GAYAALLHIERGILTDSTVYSIVAAMKQKRFGMVQRLEQYAVIYMTVLGRLGVDISGLVS TLNLKA
 * Amino Acid Sequence: **

Sequence Length: 306 aa
 * Length of your protein in Amino Acids: **

34132.3
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website **

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 29005 Abs 0.1% (=1 g/l) 0.850, assuming all pairs of Cys residues form cystines Ext. coefficient 28880 Abs 0.1% (=1 g/l) 0.846, assuming all Cys residues are reduced
 * Molar Extinction coefficient of your protein at 280 nm wavelength: **


 * TMpred graph Image (@http://www.ch.embnet.org/software/TMPRED_form.html). **

CDS Gene Sequence (paste as text only):

GC% Content for gene:

CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):

GC% Content for gene (codon optimized):

Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):

Primer design results for 'tail' primers (this is just 2 sequences):

See ProtocolTargetDiscoveryVDS.docx for more Etiologic Risk Group Categories (for pathogens): http://www.utexas.edu/research/rsc/ibc/agent_class.html#_Toc7238334 SIGMA-ALDRICH RESOURCES Enzyme Explorer @http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer.html Enzyme Classification Index (EC number) @http://www.sigmaaldrich.com/life-science/biochemicals/biochemical-products.html?TablePage=14573088 WolframAlpha @http://www.wolframalpha.com/ DrugBank @http://www.drugbank.ca/ Databases of genes/organisms: @http://www.niaid.nih.gov/Pages/default.aspx @http://eupathdb.org/eupathdb/ @https://patricbrc.vbi.vt.edu/portal/portal/patric/Home @http://www.nmpdr.org/FIG/wiki/view.cgi/Main/EssentialGenes @http://tubic.tju.edu.cn/deg/ @http://csgid.org/csgid/cake/pages/community_request_gateway @http://tdrtargets.org/ @http://gsc.jcvi.org/status.shtml Scientific Nomenclature page from Center for Disease Control (gene, protein names and abbreviations) @http://wwwnc.cdc.gov/eid/pages/scientific-nomenclature.htm Gene Information: NCBI GENE Page: @http://www.ncbi.nlm.nih.gov/gene BLAST Page: @http://blast.ncbi.nlm.nih.gov/ Protein Information: NCBI Protein Page: @http://www.ncbi.nlm.nih.gov/protein Protein Expression Website Protein Expression Paper: Primer Overlap PCR Articles Is my target good for Virtual Screening programs?
 * Resources:**