Target+-+Tyrosine-protein+phosphatase+yopH

Format for Individual Target pages (copy this list to new Target page and then fill in for your target):

 * Target (protein/gene name):** Tyrosine-protein phosphatase yopH
 * NCBI Gene # or RefSeq#:** YPPY56_4860
 * Protein ID (NP or XP #) or Wolbachia#:** P15273
 * Organism (including strain):** Yersinia enterocolitica
 * Etiologic Risk Group (see link below):**

Protein tyrosine phosphatases play a role in regulation of normal and pathological processes such as cell proliferation, differentiation, metabolism, and the survival of human diseases such as cancer and diabetes. Tyrosine-protein phosphatase yopH main function is to dephosphorylate host-proteins in Yersinia pathogenesis and contributes to the resistant’s of phagocytosis by peritoneal macrophages.
 * Background/ Disease Information (sort of like the Intro to your Mini Research Write up):**

http://www.rcsb.org/pdb/explore/explore.do?structureId=3BLT
 * Link to TDR Targets page (if present):** No page for yopH on TDR Targets
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.):**


 * Essentiality of this protein:**
 * Complex of proteins?:**
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):**

Fig 1: Reaction catalyzed by protein-tyrosine-phosphatase (3.1.3.48)
 * *EC#: 3.1.3.48 **
 * Link to BRENDA EC# page:**
 * --** Show screenshot of BRENDA enzyme mechanism schematic[[image:yoph.png width="592" height="164"]]


 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**
 * -- link to Sigma (or other company ) page for assay (see Sigma links below)**
 * -- -or link (or citation) to paper that contains assay information**
 * -- links to assay reagents (substrates) pages.**
 * --- List cost and quantity of substrate reagents, supplier, and catalog #**

code Query 1    RERPHTSGHHGAGEARATAPSTVSPYGPEARAELSSRLTTLRNTLAPATNDPRYLQACGG  60 RERPHTSGHHGAGEARATAPSTVSPYGPEARAELSSRLTTLRNTLAPATNDPRYLQACGG Sbjct 2    RERPHTSGHHGAGEARATAPSTVSPYGPEARAELSSRLTTLRNTLAPATNDPRYLQACGG  61
 * Structure Available (PDB or Homology model)**
 * -- PDB # or closest PDB entry if using homology model:** 3BLT
 * -- For Homology Model option:**
 * Query Coverage:** 100%
 * Max % Identities:** 100%
 * % Positives:** 100%
 * Chain used for homology:**
 * Show pairwise alignment of your BLASTP search in NCBI against the PDB**

Query 61   EKLNRFRDIQCRRQTAVRADLNANYIQVGNTRTIACQYPLQSQLESHFRMLAENRTPVLA  120 EKLNRFRDIQCRRQTAVRADLNANYIQVGNTRTIACQYPLQSQLESHFRMLAENRTPVLA Sbjct 62   EKLNRFRDIQCRRQTAVRADLNANYIQVGNTRTIACQYPLQSQLESHFRMLAENRTPVLA  121

Query 121  VLASSSEIANQRFGMPDYFRQSGTYGSITVESKMTQQVGLGDGIMADMYTLTIREAGQKT  180 VLASSSEIANQRFGMPDYFRQSGTYGSITVESKMTQQVGLGDGIMADMYTLTIREAGQKT Sbjct 122  VLASSSEIANQRFGMPDYFRQSGTYGSITVESKMTQQVGLGDGIMADMYTLTIREAGQKT  181

Query 181  ISVPVVHVGNWPDQTAVSSEVTKALASLVDQTAETKRNMYESKGSSAVADDSKLRPVIHC  240 ISVPVVHVGNWPDQTAVSSEVTKALASLVDQTAETKRNMYESKGSSAVADDSKLRPVIHC Sbjct 182  ISVPVVHVGNWPDQTAVSSEVTKALASLVDQTAETKRNMYESKGSSAVADDSKLRPVIHC  241

Query 241  RAGVGRTAQLIGAMCMNDSRNSQLSVEDMVSQMRVQRNGIMVQKDEQLDVLIKLAEGQGR  300 RAGVGRTAQLIGAMCMNDSRNSQLSVEDMVSQMRVQRNGIMVQKDEQLDVLIKLAEGQGR Sbjct 242  RAGVGRTAQLIGAMCMNDSRNSQLSVEDMVSQMRVQRNGIMVQKDEQLDVLIKLAEGQGR  301

Query 301  PLLNS  305 PLLNS Sbjct 302  PLLNS  306 code

RERPHTSGHHGAGEARATAPSTVSPYGPEARAELSSRLTTLRNTLAPATNDPRYLQACGGEKLNRFRDIQCRRQTAVRADLNANYIQVGNTRTIACQYPLQSQLESHFRMLAENRTPVLAVLASSSEIANQRFGMPDYFRQSGTYGSITVESKMTQQVGLGDGIMADMYTLTIREAGQKTISVPVVHVGNWPDQTAVSSEVTKALASLVDQTAETKRNMYESKGSSAVADDSKLRPVIHCRAGVGRTAQLIGAMCMNDSRNSQLSVEDMVSQMRVQRNGIMVQKDEQLDVLIKLAEGQGRPLLNS Ext. coefficient 19285 Abs 0.1% (=1 g/l) 0.379, assuming all pairs of Cys residues form cystine. Ext. coefficient 18910 Abs 0.1% (=1 g/l) 0.372, assuming all Cys residues are reduced.
 * Current Inhibitors:** []
 * Expression Information (has it been expressed in bacterial cells):** Mostly like no transmembrane protein - no possible model found according to TMPred results.
 * Purification Method :** []
 * Image of protein (PyMol with features delineated and shown separately):**
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):**
 * length of your protein in Amino Acids:** 305 residues
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website:** 50869.3
 * Molar Extinction coefficient of your protein at 280 nm wavelength:**
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
 * CDS Gene Sequence (paste as text only):**
 * GC% Content for gene:**
 * CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):**
 * GC% Content for gene (codon optimized):**

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**
 * ( link to DNA Works output text file - **that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences):**