Target+-+

PROTEIN NAME: UDP-N-acetylglucosamine pyrophosphorylase GENE NAME: AFUA_7G02180
 * Target (protein/gene name): **
 * __ http://www.ncbi.nlm.nih.gov/pubmed/23750903 __
 * __ http://www.rcsb.org/pdb/explore/explore.do?structureId=4BMA __

499141912 499141913
 * NCBI Gene # or RefSeq#: **
 * http://www.ncbi.nlm.nih.gov/protein/4BMA_A
 * http://www.ncbi.nlm.nih.gov/protein/4BMA_B

4BMA.10.2210
 * Protein ID (NP or XP #) or Wolbachia#: **

//Aspergillus fumigatus//
 * Organism (including strain): **
 * __ http://gsc.jcvi.org/projects/gsc/a_fumigatus/index.shtml __

Risk group 2 >
 * Etiologic Risk Group (see link below): **
 * http://www.absa.org/riskgroups/
 * Background/ Disease Information (sort of like the Intro to your Mini Research Write up): **

Not present for the organism //Aspergillus fumigatus//
 * Link to TDR Targets page (if present): **


 * http://www.tdrtargets.org/search?form=simple&form_submit=yes&query=UDP-N-ACETYLGLUCOSAMINE+PYROPHOSPHORYLASE


 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) **
 * Essentiality of this protein: **
 * Complex of proteins?: **
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **


 * *EC#: **
 * Link to BRENDA EC# page: **
 * -- ** Show screenshot of BRENDA enzyme mechanism schematic
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **
 * -- link to Sigma (or other company ) page for assay (see Sigma links below) **
 * -- -or link (or citation) to paper that contains assay information **
 * -- links to assay reagents (substrates) pages. **
 * --- List cost and quantity of substrate reagents, supplier, and catalog # **

-- PDB # or closest PDB entry if using homology model: -- For Homology Model option: Show pairwise alignment of your BLASTP search in NCBI against the PDB Query Coverage: Max % Identities: % Positives Chain used for homology:
 * Structure Available (PDB or Homology model) **


 * Current Inhibitors: **
 * Expression Information (has it been expressed in bacterial cells): **
 * Purification Method : **
 * Image of protein (PyMol with features delineated and shown separately): **
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **
 * *length of your protein in Amino Acids **
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website **
 * Molar Extinction coefficient of your protein at 280 nm wavelength: **
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
 * *CDS Gene Sequence (paste as text only): **
 * *GC% Content for gene: **
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **
 * *GC% Content for gene (codon optimized): **


 * __ **

Resources:
See ** ProtocolTargetDiscoveryVDS.docx ** for more Etiologic Risk Group Categories (for pathogens): http://www.utexas.edu/research/rsc/ibc/agent_class.html#_Toc7238334

Enzyme Explorer @http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer.html
 * SIGMA-ALDRICH RESOURCES **

Enzyme Classification Index (EC number) @http://www.sigmaaldrich.com/life-science/biochemicals/biochemical-products.html?TablePage=14573088

WolframAlpha @http://www.wolframalpha.com/ DrugBank @http://www.drugbank.ca/

@http://www.niaid.nih.gov/Pages/default.aspx @http://eupathdb.org/eupathdb/ @https://patricbrc.vbi.vt.edu/portal/portal/patric/Home @http://www.nmpdr.org/FIG/wiki/view.cgi/Main/EssentialGenes @http://tubic.tju.edu.cn/deg/ @http://csgid.org/csgid/cake/pages/community_request_gateway @http://tdrtargets.org/ @http://gsc.jcvi.org/status.shtml
 * Databases of genes/organisms: **

@http://wwwnc.cdc.gov/eid/pages/scientific-nomenclature.htm
 * Scientific Nomenclature page from Center for Disease Control (gene, protein names and abbreviations) **

NCBI GENE Page: @http://www.ncbi.nlm.nih.gov/gene BLAST Page: @http://blast.ncbi.nlm.nih.gov/
 * Gene Information: **

NCBI Protein Page: @http://www.ncbi.nlm.nih.gov/protein Protein Expression Website Protein Expression Paper:
 * Protein Information: **


 * Primer Overlap PCR Articles **


 * Is my target good for Virtual Screening programs? **