TargetSp15-+Triosephosphate+Isomerase+(Trypanosoma+brucei)


 * Target (protein/gene name): **triosephosphate isomerase


 * NCBI Gene # or RefSeq#: **GI: 71755425


 * Protein ID (NP or XP #) or Wolbachia#: **XP_828627.1


 * Organism (including strain): **trypanosoma brucei


 * Etiologic Risk Group (see link below): **Risk Group 2: Bacterial agents

A protozoa of the speciestrypanosoma brucei (gambiense) causes African trypanosomiasis, a sleeping sickness, in humans. It is usually transmitted by the bite of an infected  tsetse fly  found most commonly in rural areas around sub-Saharan Africa. Symptoms include fevers, headaches, itchiness, joint pains, confusion, poor coordination, numbness and trouble sleeping. Diagnosis is via finding the parasite in a  blood smear or in the fluid of a lymph node.
 * Disease Information : **


 * Link to TDR Targets page (if present): **
 * [] **


 * Link to Gene Database page: **
 * [] **

Is it a monomer or multimer as biological unit ** ? (make prediction at ** @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): monomer
 * Essentiality of this protein: **


 * Complex of proteins?: ** no

<span style="font-family: Arial,sans-serif;">[]
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **
 * <span style="font-family: Arial,sans-serif;">[] **
 * <span style="font-family: Arial,sans-serif;">EC#: ****<span style="font-family: Arial,sans-serif;">5.3.1.1 **

<span style="font-family: Arial,sans-serif;"> **<span style="font-family: Arial,sans-serif;">Link to BRENDA EC# page: ****<span style="font-family: Arial,sans-serif;">[] ** <span style="font-family: Arial,sans-serif;"> <span style="font-family: Arial,sans-serif;">

<span class="m9l40l772" style="font-family: Arial,sans-serif;">**Enzyme Assay** <span class="apple-converted-space" style="font-family: Arial,sans-serif;"> <span style="font-family: Arial,sans-serif;">**<span style="font-family: Arial,sans-serif;">information (spectrophotometric, coupled assay ?, reagents): **

<span style="font-family: Arial,sans-serif;"> **<span style="font-family: Arial,sans-serif;">-- link to Sigma (or other ** <span class="apple-converted-space" style="font-family: Arial,sans-serif;"> <span class="r7mb967" style="font-family: Arial,sans-serif;">**company** <span style="font-family: Arial,sans-serif;">**<span style="font-family: Arial,sans-serif;">) page for assay (see Sigma links below): **
 * <span style="font-family: Arial,sans-serif;">[] **

<span style="font-family: Arial,sans-serif;">[]
 * <span style="font-family: Arial,sans-serif;">-- link (or citation) to paper that contains assay information: **

<span style="font-family: Arial,sans-serif;"> **<span style="font-family: Arial,sans-serif;">-- links to assay reagents (substrates) pages: ** <span style="color: windowtext; font-family: Arial,sans-serif; font-size: 10pt;">D-Glyceraldehyde 3-phosphate = glycerone phosphate

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **<span style="font-family: Arial,sans-serif;">--- List cost and quantity of substrate reagents, supplier, and catalog #: ** <span style="color: windowtext; font-family: Arial,sans-serif; font-size: 10pt;">D-Glyceraldehyde 3-phosphate: 8-13 mg/mL in H2O ,39705-1ML, $249.00, Sigma

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **<span style="font-family: Arial,sans-serif;">Structure (PDB or Homology model) **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> PDB # or closest PDB entry if using homology model: 1AG1


 * <span style="font-family: Arial,sans-serif; font-size: 10pt;">Current Inhibitors: **<span style="font-family: Arial,sans-serif; font-size: 10pt;">2-phosphoglycolic acid (PGA), phosphoenolpyruvate (PEP)


 * <span style="font-family: Arial,sans-serif; font-size: 10pt;">Expression Information (has it been expressed in bacterial cells): **<span style="font-family: Arial,sans-serif; font-size: 10pt;">Schistosoma japonicum

**<span style="font-family: Arial,sans-serif; font-size: 10pt;">Purification Method ** **<span style="font-family: Arial,sans-serif; font-size: 10pt;">: ****<span style="font-family: Arial,sans-serif; font-size: 10pt;">[] **

<span style="font-family: Arial,sans-serif; font-size: 10pt;">

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **<span style="font-family: Arial,sans-serif;">Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): ** <span style="font-family: Arial,sans-serif;">MSKPQPIAAANWKCNGSQQSLSELIDLFNSTSINHDVQCVVASTFVHLAMTKERLSHPKFVIAAQNAIAK <span style="font-family: Arial,sans-serif;">SGAFTGEVSLPILKDFGVNWIVLGHSERRAYYGETNEIVADKVAAAVAAGFMVIACIGETLQERESGRTA <span style="font-family: Arial,sans-serif;">VVVLTQIAAIAKKLKKADWAKVVIAYEPVWAIGTGKVATPQQAQEAHALIRSWVSSKIGADVAGELRILY <span style="font-family: Arial,sans-serif;">GGSVNGKNARTLYQQRDVNGFLVGGASLKPEFVDIIKATQ <span style="font-family: Arial,sans-serif; font-size: 10pt;"> **<span style="font-family: Arial,sans-serif;">length of your protein in Amino Acids: ****<span style="font-family: Arial,sans-serif;">250 amino acids **

<span class="m9l40l772" style="font-family: Arial,sans-serif;">**Molecular Weight** <span class="apple-converted-space" style="font-family: Arial,sans-serif;"> <span style="font-family: Arial,sans-serif;">**<span style="font-family: Arial,sans-serif;">of your protein in kiloDaltons using the ** <span class="apple-converted-space" style="font-family: Arial,sans-serif;"> <span style="font-family: Arial,sans-serif;">**<span style="font-family: Arial,sans-serif;">[|Expasy ProtParam] ** <span class="apple-converted-space" style="font-family: Arial,sans-serif;"> <span style="font-family: Arial,sans-serif;">**<span style="font-family: Arial,sans-serif;">website: **26818.8 kDa <span style="font-family: Arial,sans-serif; font-size: 10pt;"> **<span style="font-family: Arial,sans-serif;">Molar ** <span class="apple-converted-space" style="font-family: Arial,sans-serif; font-size: 10pt;"> <span class="m9l40l772" style="font-family: Arial,sans-serif; font-size: 10pt;">**Extinction coefficient** <span class="apple-converted-space" style="font-family: Arial,sans-serif; font-size: 10pt;"> <span style="font-family: Arial,sans-serif; font-size: 10pt;">**<span style="font-family: Arial,sans-serif;">of your protein at 280 nm wavelength: ** <span style="font-family: Arial,sans-serif;">Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

<span style="font-family: Arial,sans-serif;">Ext. coefficient 35075 <span style="font-family: Arial,sans-serif;">Abs 0.1% (=1 g/l) 1.308, assuming all pairs of Cys residues form cystines

<span style="font-family: Arial,sans-serif;">Ext. coefficient 34950 <span style="font-family: Arial,sans-serif;">Abs 0.1% (=1 g/l) 1.303, assuming all Cys residues are reduced

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **<span style="font-family: Arial,sans-serif;">TMpred graph Image ** ( <span style="font-family: Arial,sans-serif; font-size: 10pt;">@http://www.ch.embnet.org/software/TMPRED_form.html :

<span style="font-family: Arial,sans-serif; font-size: 10pt;">atgagcaaaccgcagccgattgcggcggcgaactggaaatgcaacggcagccagcagagc <span style="font-family: Arial,sans-serif; font-size: 10pt;">ctgagcgaactgattgatctgtttaacagcaccagcattaaccatgatgtgcagtgcgtg <span style="font-family: Arial,sans-serif; font-size: 10pt;">gtggcgagcacctttgtgcatctggcgatgaccaaagaacgcctgagccatccgaaattt <span style="font-family: Arial,sans-serif; font-size: 10pt;">gtgattgcggcgcagaacgcgattgcgaaaagcggcgcgtttaccggcgaagtgagcctg <span style="font-family: Arial,sans-serif; font-size: 10pt;">ccgattctgaaagattttggcgtgaactggattgtgctgggccatagcgaacgccgcgcg <span style="font-family: Arial,sans-serif; font-size: 10pt;">tattatggcgaaaccaacgaaattgtggcggataaagtggcggcggcggtggcggcgggc <span style="font-family: Arial,sans-serif; font-size: 10pt;">tttatggtgattgcgtgcattggcgaaaccctgcaggaacgcgaaagcggccgcaccgcg <span style="font-family: Arial,sans-serif; font-size: 10pt;">gtggtggtgctgacccagattgcggcgattgcgaaaaaactgaaaaaagcggattgggcg <span style="font-family: Arial,sans-serif; font-size: 10pt;">aaagtggtgattgcgtatgaaccggtgtgggcgattggcaccggcaaagtggcgaccccg <span style="font-family: Arial,sans-serif; font-size: 10pt;">cagcaggcgcaggaagcgcatgcgctgattcgcagctgggtgagcagcaaaattggcgcg <span style="font-family: Arial,sans-serif; font-size: 10pt;">gatgtggcgggcgaactgcgcattctgtatggcggcagcgtgaacggcaaaaacgcgcgc <span style="font-family: Arial,sans-serif; font-size: 10pt;">accctgtatcagcagcgcgatgtgaacggctttctggtgggcggcgcgagcctgaaaccg <span style="font-family: Arial,sans-serif; font-size: 10pt;">gaatttgtggatattattaaagcgacccag
 * <span style="font-family: Arial,sans-serif; font-size: 10pt;">CDS ** **<span style="font-family: Arial,sans-serif; font-size: 10pt;">Gene Sequence ** **<span style="font-family: Arial,sans-serif; font-size: 10pt;">(paste as text only): **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **<span style="font-family: Arial,sans-serif;">GC% Content for gene: ****<span style="font-family: Arial,sans-serif;">57.73 % **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **<span style="font-family: Arial,sans-serif;">CDS Gene Sequence (codon optimized) - copy from ** <span class="apple-converted-space" style="font-family: Arial,sans-serif; font-size: 10pt;"> <span class="m9l40l772" style="font-family: Arial,sans-serif; font-size: 10pt;">**output** <span class="apple-converted-space" style="font-family: Arial,sans-serif; font-size: 10pt;"> <span style="font-family: Arial,sans-serif; font-size: 10pt;">**<span style="font-family: Arial,sans-serif;">of ** <span class="apple-converted-space" style="font-family: Arial,sans-serif; font-size: 10pt;"> <span class="m9l40l772" style="font-family: Arial,sans-serif; font-size: 10pt;">**Primer Design** <span class="apple-converted-space" style="font-family: Arial,sans-serif; font-size: 10pt;"> <span style="font-family: Arial,sans-serif; font-size: 10pt;">**<span style="font-family: Arial,sans-serif;">Protocol (paste as text only): **

<span style="font-family: Arial,sans-serif; font-size: 10pt;"> **<span style="font-family: Arial,sans-serif;">GC% Content for gene (codon optimized): **