TargetSp15-+Serine+threonine-protein+phosphatase+(Plasmodium+falciparum)

***NCBI Gene # or RefSeq#:** ** 813836 ** ***Protein ID (NP or XP #) or Wolbachia#:** ** 3H60 ** ***Organism (including strain):** ** Plasmodium falciparum ** **Etiologic Risk Group (see link below):** ** 2 ** ***/** **Disease Information** **(sort of like the Intro to your Mini****Research Write****up):**
 * Target (protein/gene name): **** serine/ threonine protein phosphatase **
 * Plasmodium falciparum is a parasitic protozoan that is involved in Malaria. It is the parasite responsible for roughly 85% of all Malaria cases and the most deadly of the Plasmodium protozoans. P. falciparum is carried by Anopheles mosquitoes and enters the body when a female mosquito injects it into the blood stream while feeding. From there, the P. falciparum travels to the liver via the blood stream, replicates, and then attacks the red blood cells. The parasite is carried through the bloodstream to other organs and can cause a host of symptoms including vomiting, fever, chills, diarrhea, and in severe cases, organ failure. **


 *  Serine/threonine protein phosphatase is an important target in P. falciparum. It works in part to regulate mitosis, cell division, apoptosis, and other cell functions, all of which are crucial aspects in the virulence of p. falciparum. **

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**Link to TDR Targets page (if present):** ** http://www.tdrtargets.org/targets/view?gene_id=189 ** **Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.)** ** http://plasmodb.org/plasmo/showRecord.do?name=GeneRecordClasses.GeneRecordClass&source_id=PF3D7_1355500&project_id=PlasmoDB ** **Essentiality of this protein:** ** Significant loss of fitness in bloodstream forms, significant loss of fitness in procyclic forms, significant loss of fitness in differentiation of procyclic forms ** **Is it a monomer or multimer as biological unit****? (make prediction at** ** http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html **** ): **** Monomer **** (3 amino acid chains and 6 ligands) (analysis based on protein in T. gondii) ** **Complex of proteins?:** ** No ** **Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):** ** 0.5 **

***EC#:** ** 3.1.3.16 ** **--** Show screenshot of BRENDA enzyme mechanism schematic **--Enzyme Assay** **information (spectrophotometric, coupled assay ?, reagent** ** s): Continuous or single time point spectrophotometric assay of hydrolysis of p-nitrophenyl phosphate (pNPP) ** **--Link (or citation) to paper that contains assay information** ** https://books.google.com/books?hl=en&lr=&id=Hz_2HV1GWfMC&oi=fnd&pg=PA153&dq=enzyme+assay+of+serine/threonine+phosphatase&ots=4sZj-96tE4&sig=GLqiXzO1mSK2Jb560Gl5Qp9VuvY#v=onepage&q=enzyme%20assay%20of%20serine%2Fthreonine%20phosphatase&f=false ** **--Links to assay reagents (substrates) pages. p-nitrophenyl phosphate** ** http://www.sigmaaldrich.com/catalog/product/sigma/p7998?lang=en®ion=US ** **--List cost and quantity of substrate reagents, supplier, and catalog:** **Structure (PDB or Homology model)** --PDB # or closest PDB entry if using homology model: ** 3H60 ** For Homology Model option: Show pairwise alignment of your BLASTP search in NCBI against the PDB Query Coverage: ** 80% ** Max % Identities: ** 44% ** % Positives: ** 62% ** Chain used for homology: ** Chain A ** **Current Inhibitors:** ** cyclosporine A, okadaic acid, trifluoperazine ** **Expression Information (has it been expressed in bacterial cells):** ** Yes, it has been expressed in E. coli (E. coli M15) ** ** Purification Method ** ** : **** N-terminal affinity tag was added to polypeptide. The His tag was purified through nickel-chelation chromatography. ** http://www.sciencedirect.com/science/article/pii/S0166685101002602
 * Link to BRENDA EC# page: ** ** http://www.brenda-enzymes.org/enzyme.php?ecno=3.1.3.16 **
 * Supplier: Sigma Aldrich **
 * Price/amount: $81.20 for 100mL **
 * Product Number: N2770 **
 * Image of protein (PyMol with features delineated and shown separately): **

***Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):**
 * MSKKSKDTKLIILGDVHGQLHDVLWLFNRFGIPSSTNIYIFNGDIADRGENATEIFILLF **


 * IFKLSCNDSVIINRGNHECSYMNEVYGFYNEVLSKYDNTIFDLFQNIFELLGLAVNVQNQ **


 * IFVVHGGLSRYQDITLKEIDELDRKKQEILHPEQYEDIVIFDLLWSDPQKKEGIGGNARG **


 * NNCITFGPDVTEMFLKNNNLDILIRSHQVPKTLKGIESHHEGKCITLFSASNYCNKIKNL **

***Length of your protein in Amino Acids:** ** 294 amino acids ** **Molecular Weight** **of your protein in kiloDaltons using the** [|**Expasy ProtParam**]** website: **** 33791.5 ** **Molar** **Extinction coefficient** **of your protein at 280 nm wavelength:** ** 24785 ** **TMpred graph Image** (http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it. ***CDS** **Gene Sequence** **(paste as text only):** atgagcaaaaaaagcaaagataccaaactgattattctgggcgatgtgcatggccagctgcatgatgtgctgtgg ctgtttaaccgctttggcattccgagcagcaccaacatttatatttttaacggcgatattgcggatcgcggcgaaaacgcgaccgaaatttttattctg ctgtttatttttaaactgagctgcaacgatagcgtgattattaaccgcggcaaccatgaatgcagctatatgaacgaagtgtatggcttttataacgaagtg ctgagcaaatatgataacaccatttttgatctgtttcagaacatttttgaactgctgggcctggcggtgaacgtgcagaaccagatttttgtggtgcatgg cggcctgagccgctatcaggatattaccctgaaagaaattgatgaactggatcgcaaaaaacaggaaattctgcatccggaacagtatgaagata ttgtgatttttgatctgctgtggagcgatccgcagaaaaaagaaggcattggcggcaacgcgcgcggcaacaactgcattacctttggcccggatg tgaccgaaatgtttctgaaaaacaacaacctggatattctgattcgcagccatcaggtgccgaaaaccctgaaaggcattgaaagccatcatgaag gcaaagcattaccctgtttagcgcgagcaactattgcaacaaaattaaaaacctgggcgcggcgattatttttaaccaggatctgacctttgaagtgc aggaatatatgagcccgagcctggaagtgattcgcgaaacctttgaagaaaaccagaaactgcgcgaaaaagtgctgcattgcagcaaaattgtg gaactggaaaaaaacgtgagcacc ***GC% Content for gene:** ** 44.61% **
 * GAAIIFNQDLTFEVQEYMSPSLEVIRETFEENQKLREKVLHCSKIVELEKNVST **