Target+Sp17-+Dihydropteroate+diphosphorylase+in+Salmonella+enteritidis


 * Target:** Dihydropteroate diphosphorylase



Substrates not readily available - Dr. B 060617

GO:0004156 Definition Catalysis of the reaction: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate
 * EC Number:** 2.5.1.15
 * Protein ID:** 9055-61-2
 * Organism:** Salmonellaa enteritidis
 * Etiological risk group:** N/A
 * Gene Ontology:**
 * Disease Information:**Salmonella enteritidis is responsible for fever, abdominal cramps, and diarrhea. It is common in food poisoning and causes a typhoid-like disease in mice. It is transmitted through contaminated water, contact with others, and through food. There is no cure for these strains of Salmonella as they eventually leave the body. However, mutant strains could lead to epidemics such as the recent outbreak of salmonella through consumption of peanut butter. Widespread contamination can cause problems for several groups of people and could result in recall of products.

[|Science Direct Article] [|CDC] [|Medicine Net]
 * Links:**


 * Link to TDR Targets:** Not present

This enzyme dihydropteroate in bacteria and is essential to the folate biosynthesis pathway. Cofactor production is essential for metabolism. diphosporylase is vital for catalyzing the condensation of 6-hydroxymethyl-7,8-dihydropteridine pyrophosphate to para-aminobenzoic acid to form 7,8-dihydropteroate.
 * Essentiality of Target:** ExPasy link: [|Expasy]


 * Druggable Target: []**
 * Link to BRENDA: [|BRENDA EC Number and Enzyme Information]**
 * Enzyme Assay: [|Label-free Fluorescence]**


 * 3D Structure: [|Link to PDB] (vibrio** **fisheri synonymous structure)**

code Ext. coefficient    5960 Abs 0.1% (=1 g/l)  0.198, assuming all pairs of Cys residues form cystines code code 30126.25 kDa code 10 20 30 40 50 MVTVFGILNL TEDSFFDESR RLDPAGAVTA AIEMLRVGSD VVDVGPAASH 60 70 80 90 100 PDARPVSPAD EIRRIAPLLD ALSDQMHRVS IDSFQPETQR YALKRGVGYL 110 120 130 140 150 NDIQGFPDPA LYPDIAEADC RLVVMHSAQR DGIATRTGHL RPEDALDEIV 160 170 180 190 200 RFFEARVSAL RRSGVAADRL ILDPGMGFFL SPAPETSLHV LSNLQKLKSA 210 220 230 240 250 LGLPLLVSVS RKSFLGATVG LPVKDLGPAS LAAELHAIGN GADYVRTHAP 260 270 GDLRSAITFS ETLAKFRSRD ARDRGLDHA
 * Amino Acid Length:** 279
 * Extinction Coefficient:** [|Expasy]
 * Molecular Weight:** [|Expasy]
 * Amino Acid Sequence: [|UniPro]**

Pairwaise BLASTP in NCBI: [|Link to BLASTP] **Query Coverage:** 99% **Max % Identities:** 99% **% Positives:** 99% **Chain used for homology:** A

TMpred Graph : [|Output graph]




 * Inhibitor:** Sulfadizine
 * CDS Gene Sequence:** [|Sequence Information]

code >ENA|EU934512|EU934512.2 Salmonella enterica subsp. enterica serovar Senftenberg strain SRC119 plasmid pSRC119-1 class 1 integron, partial sequence. GACGATGCGTGGAGACCGAAACCTTGCGCTCGTTCGCCAGCCAGGACAGAAATGCCTCGA CTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAA CCCAGTTGACATAAGCCTGTTCGGTTCGTAAACTGTAATGCAAGTAGCGTATGCGCTCAC GCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCA TGGCTTGTTATGACTGTTTTTTTGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGC AAGCAGCTATCGTTTTTGCAGTGCTGTGCCGCCTCGGTGGCGCAGCGTTTTTTCACGGTT AGCGCCCGTCGCCAAATTCAAGTTATCCGTTTTGGCTTCTGGTTCTAACATTTCGGTCAA GCCGACCCGCATTCTGCGGTCGGCTTACCTCGCCCGTTAGACATCATGAGGGAAGCGGTG ACCATCGAAATTTCGAACCAACTATCAGAGGTGCTAAGCGTCATTGAGCGCCATCTGGAA TCAACGTTGCTGGCCGTGCATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCATAC AGCGATATTGATTTGTTGGTTACTGTGGCCGTAAAGCTTGATGAAACGACGCGGCGAGCA TTGCTCAATGATCTTATGGAGGCTTCGGCTTTCCCTGGCGAGAGCGAGACGCTCCGCGCT ATAGAAGTCACCCTTGTCGTGCATGACGACATCATCCCGTGGCGTTATCCGGCTAAGCGC GAGCTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCGGGTATCTTCGAGCCAGCC ATGATCGACATTGATCTAGCTATCCTGCTTACAAAAGCAAGAGAACATAGCGTTGCCTTG GTAGGTCCGGCAGCGGAGGAATTCTTTGACCCGGTTCCTGAACAGGATCTATTCGAGGCG CTGAGGGAAACCTTGAAGCTATGGAACTCGCAGCCCGACTGGGCCGGCGATGAGCGAAAT GTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAATAACCGGCAAAATCGCGCCGAAG GATGTCGCTGCCGACTGGGCAATAAAACGCCTACCTGCCCAGTATCAGCCCGTCTTACTT GAAGCTAAGCAAGCTTATCTGGGACAAAAAGAAGATCACTTGGCCTCACGCGCAGATCAC TTGGAAGAATTTATTCGCTTTGTGAAAGGCGAGATCATCAAGTCAGTTGGTAAATGATGT CTAACAATTCGTTCAAGCCGACCGCGCTACGCGCGGCGGCTTAACTCCGGCGTTAGATGC ACTAAGCACATAATTGCTCACAGCCAAACTATCAGGTCAAGTCTGCTTTTATTATTTTTA AGCGTGCATAATAAGCCCTACACAAATTGGGAGATATATCATGAAAGGCTGGCTTTTTCT TGTTATCGCAATAGTTGGCGAAGTAATCGCAACATCCGCATTAAAATCTAGCGAGGGCTT TACTAAGCTTGCCCCTTCCGCCGTTGTCATAATCGGTTATGGCATCGCATTTTATTTTCT TTCTCTGGTTCTGAAATCCATCCCTGTCGGTGTTGCTTATGCAGTCTGGTCGGGACTCGG CGTCGTCATAATTACAGCCATTGCCTGGTTGCTTCATGGGCAAAAGCTTGATGCGTGGGG CTTTGTAGGTATGGGGCTCATAATTGCTGCCTTTTTGCTCGCCCGATCCCCATCGTGGAA GTCGCTGCGGAGGCCGACGCCATGGTGACGGTGTTCGGCATTCTGAATCTCACCGAGGAC TCCTTCTTCGATGAGAGCCGGCGGCTAGACCCCGCCGGCGCTGTCACCGCGGCGATCGAA ATGCTGCGAGTCGGATCAGACGTCGTGGATGTCGGACCGGCCGCCAGCCATCCGGACGCG AGGCCTGTATCGCCGGCCGATGAGATCAGACGTATTGCGCCGCTCTTAGACGCCCTGTCC GATCAGATGCACCGTGTTTCAATCGACAGCTTCCAACCGGAAACCCAGCGCTATGCGCTC AAGCGCGGCGTGGGCTACCTGAACGATATCCAAGGATTTCCTGACCCTGCGCTCTATCCC GATATTGCTGAGGCGGACTGCAGGCTGGTGGTTATGCACTCAGCGCAGCGGGATGGCATC GCCACCCGCACCGGTCACCTTCGACCCGAAGACGCGCTCGACGAGATTGTGCGGTTCTTC GAGGCGCGGGTTTCCGCCTTGCGACGGAGCGGGGTCGCTGCCGACCGGCTCATCCTCGAT CCGGGGATGGGATTTTTCTTGAGCCCCGCACCGGAAACATCGCTGCACGTGCTGTCGAAC CTTCAAAAGCTGAAGTCGGCGTTGGGGCTTCCGCTATTGGTCTCGGTGTCGCGGAAATCC TTCTTGGGCGCCACCGTTGGCCTTCCTGTAAAGGATCTGGGTCCAGCGAGCCTTGCGGCG GAACTTCACGCGATCGGCAATGGCGCTGACTACGTCCGCACCCACGCGCCTGGAGATCTG CGAAGCGCAATCACCTTCTCGGAAACCCTCGCGAAATTTCGCAGTCGCGACGCCAGAGAC CGAGGGTTAGATCATGCCTAGCATTCACCTTCCGGCCGCCCGCTAGCGGACCCTGGTCAG GTTCCGCGAAGGTGGGCGCAGACATGCTGGGCTCGTCAGGATCAAACTGCACTATGAGGC GGCGGTTCATACCGCGCCAGGGGAGCGAATGGACAGCGAGGAGCCTCCGAACGTTCGGGT CGCCTGCTCGGGTGATATCGACGAGGTTGTGCGGCTGATGCACGACGCTGCGGCGTGGAT GTCCGCCAAGGGAACGCCCGCCTGGGACGTCGCGCGGATCGACCGGACATTCGCGGAGAC CTTCGTCCTGAGATCCGAGCTCCTAGTCGCGAGTTGCAGCGACGGCATCGTCGGCTGTTG CACCTTGTCGGCCGAGGATCCCGAGTTCTGGCCCGACGCCCTCAAGGGGGAGGCCGCATA TCTGCACAAGCTCGCGGTGCGACGGACACATGCGGGCCGGGGTGTCAGCTCCGCGCTGAT CGAGGCTTGCCGCCATGCCGCGCGAACGCAGGGGTGCGCCAAGCTGCGGCTCGACTGCCA CCCGAACCTGCGTGGCCTATACGAGCGGCTCGGATTCACCCACGTCGACACTTTCAATCC CGGCTGGGATCCAACCTTCATCGCAGAACGCCTAGAACTCGAAATCTAACGTCCGTTCGG GCATCGAGGTCCATGTCGGGGTGGGACGGGCCCGTGGCTTCAAGATCACTTGCAGTCCGA CCGCGATGTCTTGGTTGCGCGAGAGGTTGTCGACAGGCCATGCTGAGTGTGCGATGGTTG ATCGCTTCCTCGCCGCTCTCCACGGCGACGATGGCCGCCGCCATCAGCAAGTGCGCCAGT TCCCCTATGGTGCCCTCGCTGCGTGTGAGCAGGTAGCGAGCCATGTCCAGCGTGGCAATT GGGGAAGGCCGGCGCAGCGGGAGCGAAGCGGCGAAGCTGGCCAGCAGTGAGCAGCAATCG TCGTTGGCCTCCCATACCGGCAGCATCATCGGCTCGAAGCGATTTTCCAACTGGTCATCG GAGCGGATGGCTAGGTAGGCGTCGCGCGTGCCTACCCCAACCAACGGGATGCGCAGTTCG TTGCCGAGGAAGCGCAGCAGGTTGAGGAATTC

code
 * GC% Content for gene:** 6.776895%
 * CDS Gene Sequence (codon optimized) - N/a**
 * GC% Content for gene (codon optimized): N/a**