Target+-+1-deoxy-D-xylulose+5-phosphate+reductoisomerase+(Wolbachia)

MHS Rank: 106 GCS Rank: 476 on WOLTR spreadsheet essential in 3 studies done on E.coli [] [] No assay found for //Wolbachia.// Spectrophotometric assay done in //Pseudomonas aeruginosa. // [] 1-Deoxy-D-xylulose-5-phosphate sodium salt Product #: 13368 1 mg costs $66.30 [] Known druggable targets in E.coli and synechocystis [] fosmidomycin .0035mM
 * Target (protein/gene name):** 1-deoxy-D-xylulose 5-phosphate reductoisomerase
 * NCBI Gene # or RefSeq#:** 3188958
 * Protein ID (NP or XP #) or Wolbachia#:** Q5GTA4
 * Organism:** Wolbachia
 * Etiologic Risk Group (see link below):** none listed
 * Background/Disease Information:** Wolbachia infects isopods, mites, spiders, and nematodes. The filarial worms Wolbachia infects cause diseases such as heartworm in dogs and River Blindness and Elephantitis in humans
 * Essentiality of this protein:** []
 * Complex of enzymes:** No
 * EC#:** 1.1.1.267
 * Link to BRENDA EC# page:**
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**
 * -- link to Sigma (or other company) page for assay or assay reagents (substrates)**
 * -- link to paper that contains assay information** not found
 * Structure Available (PDB or Homology model):** http://www.rcsb.org/pdb/explore/explore.do?structureId=1r0k
 * -- PDB # or closest PDB entry if using homology model:** 1R0K (organism: Zymomonas mobilis)
 * -- Query Coverage (if not direct match):** 98%
 * -- Max Ident (if not direct match):** 43%
 * Druggable Target (see Databases for this):**
 * Current Inhibitors:**
 * (in E.coli)**

(2R,3R)-2,3,4-trihydroxybutyl dihydrogen phosphate .310 mM IC50 none found []
 * Expression Information (has it been expressed in bacterial cells):**
 * Purification Method:**


 * Purification/COMMENTARY || ORGANISM || LITERATURE ||
 * by immobilized metal affinity chromatography on a Co column, followed by anion exchange chromatography, gel filtration and ultrafiltration || [|Escherichia coli] || 697249 ||
 * of recombinant protein || [|Escherichia coli] || 286423, 286427 , 286428 ||
 * recombinant His-tagged enzyme from overexpressing strain BL21 by nickel affinity chromatography || [|Escherichia coli] || 672040 ||

[]
 * Image of protein (PyMol or etc):**
 * 1ROK, PDB**
 * Amino Acid Sequence:**

>sp|Q5GTA4|DXR_WOLTR 1-deoxy-D-xylulose 5-phosphate reductoisomerase OS=Wolbachia sp. subsp. Brugia malayi (strain TRS) GN=dxr PE=3 SV=1 MKKISVLGSTGSIGKKTVDLLLKRKEEYQVEALSTCSNFALLACQAKLLNARYVAISNKR FYKDLKESLLGTGIKVEVGTEGLMNVASLPVDLSVVAVVGIAGLEPVMHVIESGTKVIAL ANKESIVCGGKLLLKKTEEKNVQIVPIDSEHNAIFQVLQNGNKCVEKIILTASGGSFLNY SLEQLRNVTVGQALSHPTWNMGKKISVDSATMMNKALEIIEAHNLFNISPNRIEAIVHPE SIIHGIVIYKDGFNFAVLAEADMAIPISYALSWPERSALSYKLDLTKQKLTFQEPDHKRF PALKLSMEVLNSSSPHTNSIVLNAANEVAVDKFLKSRIDFLEIIKVVKLTVENFDSYTDI NSLSDIINIDLESRAIAKEIIKNKVLAYS code format="sequence" 1**__0__**        2**__0__**         3**__0__**         4**__0__**         5**__0__**         6**__0__** MKKISVLGST GSIGKKTVDL LLKRKEEYQV EALSTCSNFA LLACQAKLLN ARYVAISNKR

7**__0__**        8**__0__**         9**__0__**        10**__0__**        11**__0__**        12**__0__** FYKDLKESLL GTGIKVEVGT EGLMNVASLP VDLSVVAVVG IAGLEPVMHV IESGTKVIAL

13**__0__**       14**__0__**        15**__0__**        16**__0__**        17**__0__**        18**__0__** ANKESIVCGG KLLLKKTEEK NVQIVPIDSE HNAIFQVLQN GNKCVEKIIL TASGGSFLNY

19**__0__**       20**__0__**        21**__0__**        22**__0__**        23**__0__**        24**__0__** SLEQLRNVTV GQALSHPTWN MGKKISVDSA TMMNKALEII EAHNLFNISP NRIEAIVHPE

25**__0__**       26**__0__**        27**__0__**        28**__0__**        29**__0__**        30**__0__** SIIHGIVIYK DGFNFAVLAE ADMAIPISYA LSWPERSALS YKLDLTKQKL TFQEPDHKRF

31**__0__**       32**__0__**        33**__0__**        34**__0__**        35**__0__**        36**__0__** PALKLSMEVL NSSSPHTNSI VLNAANEVAV DKFLKSRIDF LEIIKVVKLT VENFDSYTDI

37**__0__**       38**__0__**NSLSDIINID LESRAIAKEI IKNKVLAYS code http://www.ch.embnet.org/software/TMPRED_form.html
 * TMPRED**
 * put TmPred result image here .............**

[]

[]
 * length of your protein in Amino Acids:** 389
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam]website**

code 42738.7 code code Ext. coefficient   24410 code []
 * Molar Extinction coefficient of your protein at 280 nm wavelength:**
 * CDS Gene Sequence:**
 * 1167 bp**

code GTGAAAAAGATTTCAGTTTTAGGATCGACAGGAAGTATTGGAAAAAAGACTGTAGATTTACTATTAAAGA GAAAAGAAGAATATCAAGTAGAAGCACTCAGTACCTGTTCGAATTTTGCTCTACTGGCATGCCAAGCAAA ACTGCTAAATGCAAGGTATGTTGCTATCTCTAATAAAAGGTTTTATAAAGATTTAAAAGAAAGTCTACTT GGTACGGGCATCAAAGTAGAAGTTGGTACTGAGGGTCTAATGAATGTTGCTTCTCTGCCCGTTGATCTCT CAGTTGTTGCAGTAGTTGGTATTGCAGGCCTTGAGCCAGTAATGCACGTCATAGAAAGTGGTACTAAAGT CATTGCACTAGCAAACAAAGAAAGTATTGTTTGTGGTGGAAAACTATTACTTAAGAAAACCGAAGAAAAA AACGTACAGATAGTTCCTATTGACTCTGAACACAATGCAATTTTTCAAGTTTTGCAGAATGGCAATAAAT GTGTAGAAAAGATTATACTCACTGCTTCTGGTGGGTCATTCTTGAATTATAGCCTTGAGCAATTAAGAAA CGTTACAGTAGGTCAAGCACTTAGTCATCCTACTTGGAACATGGGAAAGAAAATCTCAGTTGATAGTGCA ACTATGATGAACAAAGCGCTGGAGATAATAGAAGCACATAATTTATTTAATATTAGCCCAAACAGAATTG AAGCAATAGTGCATCCCGAATCGATAATACATGGAATTGTAATTTATAAGGATGGTTTTAATTTTGCTGT ACTTGCAGAGGCTGATATGGCAATTCCCATTTCATATGCTCTGTCTTGGCCAGAAAGGTCAGCTTTAAGT TATAAATTAGATTTAACAAAGCAGAAATTGACCTTTCAAGAACCGGACCATAAGCGCTTTCCTGCGCTAA AGCTTAGTATGGAAGTGTTAAATTCCTCCTCTCCACACACAAATAGCATAGTACTTAATGCTGCAAATGA GGTAGCTGTTGATAAATTTTTAAAATCACGAATTGACTTTTTGGAGATAATAAAAGTAGTAAAGTTAACA GTAGAGAATTTTGATAGTTACACTGACATTAACTCATTATCCGATATAATAAACATTGATCTTGAAAGCC GTGCTATTGCAAAAGAAATTATCAAAAATAAAGTTCTTGCGTATAGCTAA code

[] []
 * GC% Content for gene: 35.45%**
 * CDS Gene Sequence (codon optimized) - after having done Primer Design:**

ATG __AAGAAAATTTCCGTTCTG__GGTTCTACCGGCTCTATCGGTAAAAAGACCGTAGATCTGCTGCTGAAACGCAAAGAAGAATACCAGGTTGAAGCGCTGTCTACCTGCTCTAACTTCGCGCTGCTCGCGTGTCAGGCGAAACTGCTCAATGCCCGCTATGTTGCGATCTCTAACAAACGCTTTTACAAAGACCTGAAAGAGTCTCTGCTGGGTACCGGCATTAAAGTTGAAGTTGGTACCGAAGGTCTGATGAACGTAGCGTCTCTCCCGGTTGACCTGTCTGTTGTCGCAGTTGTTGGTATCGCGGGTCTGGAACCAGTTATGCATGTCATCGAATCTGGTACGAAAGTTATCGCGCTCGCGAACAAAGAATCTATCGTTTGCGGTGGTAAACTCCTCCTCAAGAAAACTGAAGAGAAAAACGTCCAGATCGTGCCGATCGACAGCGAGCACAACGCGATCTTCCAGGTACTGCAAAACGGCAACAAATGTGTCGAAAAAATCATCCTGACCGCGTCTGGTGGTTCCTTCCTGAATTACTCTCTCGAACAGCTGCGTAATGTTACCGTAGGTCAGGCCCTGTCTCACCCGACGTGGAACATGGGCAAAAAGATCTCCGTTGACTCTGCGACCATGATGAATAAGGCGCTCGAAATTATCGAGGCACACAACCTGTTCAACATCTCTCCGAACCGTATCGAAGCGATCGTTCACCCGGAGTCTATCATCCACGGTATCGTTATCTACAAGGACGGTTTCAATTTCGCCGTGCTGGCGGAAGCGGACATGGCGATCCCGATTTCTTATGCACTGTCTTGGCCGGAGCGTTCCGCGCTGAGCTACAAACTGGACCTGACCAAACAGAAACTGACTTTCCAAGAACCGGACCATAAACGCTTCCCGGCACTGAAACTGTCTATGGAAGTTCTCAACTCTTCTTCTCCGCACACCAACAGCATCGTTCTGAACGCTGCGAACGAAGTTGCAGTGGACAAATTTCTGAAATCTCGCATCGATTTCCTGGAGATTATCAAAGTTGTTAAACTCACCGTTGAAAACTTCGACTCTTATACCGACATCAACTCCCTCTCTGACATCATTAACATTGACCTCGAAAGCCGTGCGATTGCAAAAGAGATCATCAAAAACAAA__GTTCTGGCGTACTCT__ TAA

[] 48.46%
 * GC% Content for gene (codon optimized):**


 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**


 * Primer design results for 'tail' primers (this is just 2 sequences):**

FORWARD PRIMER TACTTCCAATCC __ ATG __ __ AAGAAAATTTCCGTTCTG __ REVERSE PRIMER TATCCACCTTTACTG__ TTA ____ AGAGTACGCCAGAAC __ //__ Underlined: __// 18 bp from CDS // Highlighted: // start and stop codons


 * Primer Sequence for 1-deoxy-D-xylulose 5- phosphate reductoisomerase (Wolbachia endosymiont strain TRS of Brugia Malayi)**

[] see oligonucleotide sequences