Target+-+Serine-threonine+specific+protein+phosphatase+(Taxoplasma+Gondii)


 * Target (protein/gene name):** Serine/threonine specific protein phosphatase


 * NCBI Gene # or RefSeq#:** NCBI gene # 5811


 * Protein ID (NP or XP #) or Wolbachia#:** None on PDB Page


 * Organism (including strain):** Toxoplasma gondii (strain ME49)


 * Etiologic Risk Group (see link below): Group A**

A single-celled parasite called //Toxoplasma gondii // causes a disease known as toxoplasmosis. While the parasite is found throughout the world, more than 60 million people in the United States may be infected with the //Toxoplasma // parasite. Of those who are infected, very few have symptoms because a healthy person's immune system usually keeps the parasite from causing illness. However, pregnant women and individuals who have compromised immune systems should be cautious; for them, a //Toxoplasma // infection could cause serious health problems. [1]
 * Background/ Disease Information (sort of like the Intro to your Mini Research Write up):**

http://www.tdrtargets.org/search?form=simple&form_submit=yes&query=Toxoplasma+gondii
 * Link to TDR Targets page:**

http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?lvl=0&id=5811
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.)**

** [|GO:0000159] protein phosphatase type 2A complex**
 * Essentiality of this protein:**
 * Gene Ontology**

Function: (mouse over links to read term descriptions) ** [|GO:0008601] protein phosphatase type 2A regulator activity** ** [|GO:0005488] binding**


 * Complex of proteins?: No**


 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):**
 * No chemical compounds**


 * EC#: 3.1.3.16**


 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**
 * -- link to Sigma (or other company ) page for assay (see Sigma links below)**
 * -- -or link (or citation) to paper that contains assay information**
 * -- links to assay reagents (substrates) pages.**
 * --- List cost and quantity of substrate reagents, supplier, and catalog #**

-- PDB # or closest PDB entry if using homology model: -- For Homology Model option: Show pairwise alignment of your BLASTP search in NCBI against the PDB Query Coverage: Max % Identities: % Positives Chain used for homology:
 * Structure Available (PDB or Homology model)**


 * Current Inhibitors:** No chemical compounds associated to this protein


 * Expression Information (has it been expressed in bacterial cells):** No


 * Purification Method :** Affinity Chromatography 6 his tag

No PyMol image of protein
 * Image of protein (PyMol with features delineated and shown separately):**


 * Amino Acid Sequence:**

MAGTSIFQGSSWARILRDAVQIIPGRFFFLSLTGTPWDTQSIHFFCTDQTFQYEPFFADF GPLSLGCIYKYAKLLESKLKEAEERQHILIHYSAIHPEKRANAALLIGAAQMLLFGMSAQ EAYRPFLNISPRFVPFRDATCGPCNFKLTILDCLKGLEFAMKLGWFDYKTFNVDEYDYYE KLKNGDMNWIIPSRILAFSCPSSATGNHDGYSTCTPEDYANIFNSLGIKTVVRLNKKQYD ARKFTDRNIEHVDLFFVDGTCPSREIIQAFLQVVENRDHPIAVHCKAGLGRTGTLIGCYA IKNFKFPAVEWIGWNRLCRPGSILGPQQQFLTEIQHELLQMNRENSIHTRRLHASQPSKS LPSSSPSQKDDLADCLAKLSLDSRRVAEHGDAGQGERLLVAKRQQQAGLSTAASSTSSSV NGIDSGSQRKILPLPLLLSRPLSSNTAPFSEVTVLFNVHLLKLHTIRSLTFCRGIVSRC


 * length of protein in Amino Acids:** 479


 * Molecular Weight of protein in kiloDaltons:** 53793.6


 * Molar Extinction coefficient of your protein at 280 nm wavelength:** 53245


 * TMpred graph Image**


 * CDS Gene Sequence :**

ATGGCAGGGACAAGCATTTTTCAAGGCTCTTCATGGGCTCGCATTCTTCGCGATGCGGTC CAGATCATTCCAGGTCGTTTCTTTTTCCTCTCACTGACTGGCACTCCTTGGGATACCCAG TCCATACACTTCTTCTGCACAGATCAGACATTCCAGTATGAGCCATTCTTCGCAGACTTC GGACCTCTTAGTCTTGGTTGCATTTACAAATATGCAAAACTTCTTGAATCCAAGCTCAAA GAAGCAGAGGAACGGCAACACATTTTGATACACTATTCGGCCATTCATCCTGAAAAGCGA GCAAATGCTGCGCTCTTAATCGGGGCAGCTCAGATGCTTCTCTTCGGAATGTCCGCTCAA GAGGCATACAGGCCGTTTCTTAATATTTCACCTCGATTCGTTCCTTTCCGGGATGCAACA TGCGGACCGTGTAATTTCAAGCTGACAATTTTGGACTGCCTTAAGGGTCTGGAATTCGCG ATGAAACTTGGATGGTTTGACTACAAGACCTTCAATGTTGACGAGTACGACTACTATGAA AAGTTGAAAAACGGAGATATGAATTGGATTATCCCAAGCCGTATCCTGGCATTCTCTTGC CCTTCCAGCGCCACAGGCAACCACGACGGATACAGCACGTGCACCCCAGAAGACTACGCG AATATTTTCAATAGCCTAGGGATTAAAACAGTCGTACGGTTGAACAAGAAACAATATGAT GCCAGGAAGTTCACCGACAGGAACATCGAGCACGTGGATCTATTTTTCGTAGATGGTACC TGCCCCTCCAGAGAAATAATACAAGCATTTCTGCAGGTGGTCGAAAACCGAGATCATCCA ATTGCGGTGCACTGTAAAGCTGGCCTTGGCCGAACAGGGACCCTCATCGGATGCTATGCC ATCAAAAACTTCAAGTTCCCCGCAGTTGAGTGGATAGGCTGGAACCGATTGTGCAGGCCG GGTAGCATTTTGGGGCCACAACAGCAATTCCTTACTGAAATACAGCACGAGTTGCTTCAA ATGAATCGGGAGAATTCTATACACACACGCCGATTACATGCTTCACAGCCATCGAAATCG CTGCCAAGCTCTTCTCCGTCTCAAAAGGACGATCTTGCCGATTGCTTAGCGAAACTTTCC CTGGACAGTCGGCGAGTGGCGGAACATGGAGACGCTGGACAAGGCGAGCGACTGCTCGTT GCCAAGCGGCAACAGCAGGCAGGGCTGTCTACTGCTGCATCGTCTACGTCGTCAAGTGTA AATGGCATTGACTCGGGGTCCCAAAGAAAGATCCTTCCCCTTCCTCTTCTTCTCTCCCGT CCTCTATCTAGCAATACTGCTCCGTTCTCAGAAGTGACGGTGCTATTCAATGTGCATCTT TTGAAGCTTCACACCATCAGAAGCCTCACATTCTGTAGGGGAATTGTCTCGCGCTGTTGA


 * GC% Content for gene: 48.6%**

ATGGCCGGAA CCTCTATTTT TCAGGGTTCT TCTTGGGCCC GGATTCTTCG CGATGCGGTC CAGATTATTC CAGGCCGTTT CTTTTTCCTC TCCCTGACTG GTACCCCCTG GGATACGCAG TCCATTCACT TCTTTTGCAC AGATCAAACG TTCCAGTACG AGCCTTTTTT CGCGGATTTT GGTCCATTGA GCTTAGGCTG TATCTACAAG TATGCCAAAT TATTAGAGTC AAAATTAAAA GAAGCAGAAG AGCGTCAGCA CATTCTCATC CATTACAGCG CAATTCACCC GGAAAAACGC GCCAATGCTG CATTGCTGAT TGGTGCGGCC CAGATGCTGC TTTTTGGAAT GAGCGCGCAG GAGGCATACC GCCCGTTTCT GAACATCAGC CCTCGGTTTG TGCCATTTCG TGATGCGACC TGCGGCCCTT GTAATTTTAA ATTGACGATC CTTGATTGCC TGAAAGGCCT GGAATTTGCC ATGAAACTGG GTTGGTTTGA CTATAAGACG TTCAATGTGG ACGAATACGA CTACTATGAA AAGTTAAAAA ACGGCGATAT GAATTGGATT ATTCCTAGTC GCATTCTGGC GTTCAGTTGT CCGTCCAGCG CGACGGGCAA CCATGATGGC TACTCCACGT GTACCCCGGA AGATTACGCA AATATTTTCA ACTCCCTGGG CATTAAAACC GTTGTTCGTT TAAATAAAAA ACAGTATGAC GCGCGCAAGT TCACTGATCG TAACATTGAG CATGTTGATC TGTTCTTTGT CGACGGGACA TGCCCGAGTC GGGAAATCAT CCAAGCGTTC CTGCAGGTTG TCGAAAACCG CGATCATCCG ATCGCGGTTC ATTGCAAAGC CGGCCTTGGC CGTACCGGCA CACTGATTGG TTGCTATGCG ATTAAGAATT TCAAATTCCC GGCCGTAGAA TGGATCGGAT GGAACCGGCT TTGCCGTCCG GGTAGCATTT TGGGGCCACA GCAGCAGTTC CTTACGGAGA TTCAGCACGA GCTCCTGCAG ATGAATCGGG AGAACTCGAT TCATACTCGT CGTCTGCATG CAAGCCAGCC GTCTAAAAGT CTGCCGTCAT CTAGCCCATC CCAGAAAGAT GACCTGGCTG ACTGTCTTGC GAAACTGTCA CTCGATAGCC GCCGTGTAGC AGAGCATGGA GACGCCGGGC AAGGTGAACG TCTGCTGGTT GCTAAACGCC AGCAACAGGC TGGTCTGTCA ACCGCAGCTT CTTCGACCTC CTCGAGTGTT AATGGCATCG ATTCAGGATC GCAACGTAAA ATCCTGCCAT TGCCGCTGCT CCTGTCCCGT CCTCTGTCAA GCAACACCGC GCCTTTCTCG GAGGTCACCG TTTTATTTAA CGTGCACCTG CTGAAATTAC ACACAATTCG CTCCCTCACC TTCTGTCGTG GCATTGTTTC CCGTTGTTAA
 * CDS Gene Sequence (codon optimized):**


 * GC% Content for gene (codon optimized): 50.1%**

Resources:
See ** ProtocolTargetDiscoveryVDS.docx ** for more

Etiologic Risk Group Categories (for pathogens): http://www.utexas.edu/research/rsc/ibc/agent_class.html#_Toc7238334


 * SIGMA-ALDRICH RESOURCES **

Enzyme Explorer

@http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer.html

Enzyme Classification Index (EC number)

@http://www.sigmaaldrich.com/life-science/biochemicals/biochemical-products.html?TablePage=14573088

WolframAlpha @http://www.wolframalpha.com/

DrugBank @http://www.drugbank.ca/


 * Databases of genes/organisms: **

@http://www.niaid.nih.gov/Pages/default.aspx

@http://eupathdb.org/eupathdb/

@https://patricbrc.vbi.vt.edu/portal/portal/patric/Home

@http://www.nmpdr.org/FIG/wiki/view.cgi/Main/EssentialGenes

@http://tubic.tju.edu.cn/deg/

@http://csgid.org/csgid/cake/pages/community_request_gateway

@http://tdrtargets.org/

@http://gsc.jcvi.org/status.shtml


 * Scientific Nomenclature page from Center for Disease Control (gene, protein names and abbreviations) **

@http://wwwnc.cdc.gov/eid/pages/scientific-nomenclature.htm


 * Gene Information: **

NCBI GENE Page: @http://www.ncbi.nlm.nih.gov/gene

BLAST Page: @http://blast.ncbi.nlm.nih.gov/


 * Protein Information: **

NCBI Protein Page: @http://www.ncbi.nlm.nih.gov/protein

Protein Expression Website

Protein Expression Paper:


 * Primer Overlap PCR Articles **






 * Is my target good for Virtual Screening programs? **