Timeline+Vets

TIMELINE FOR RESEARCH (VETS)

FROM FALL 11 PyMol Refresher - Due Tuesday 9/12 Science Works assignment due Friday Sept 16th - see announcement page
 * 3rd Week (9/12 – 9/16) **

Lab Notebook Check this Friday - leave your notebook in 'Drug Screening Notebooks' drawer in lab by 7:00 pm


 * should include all current results and commentary

Wikispaces check on each Friday


 * 4th Week (9/19 – 9/23) **

Virtual Screen Refresher - Due 9/20 (see Announcement page for Virtual Screen Help Session on Thursday 9/14)
 * ProtocolVirtualScreenRefresherFall11.doc
 * Help Sessions: PyMol and Virtual Screen Refresherin Welch Computer Lab
 * Monday Sept 19th: 5-7 pm

Wikispaces check on each Friday

Finish cloning your gene into pNIC-Bsa4
 * Week 5 (9/26 – 9/30) **

For Virtual Refresher: Since the blades are full - you only need to have your first Run started by the end of this week. (by Friday 9/30)

Begin **real** Virtual Screening - prepare protein in GOLD and start jobs (cb306 should be done this week and the next one started) Wikispaces check on each Friday

Transform positive clone with your gene into BL21(De3) cells - begin expression Continue **real** Virtual Screening - prepare protein in GOLD and start jobs
 * Week 6 (10/3 – 10/7) **

Wikispaces check on each Friday
 * Lab Notebook check this Friday** (7:00 pm)

10/03/11 - Still need PyMol Refresher from: **Joey O, Larry H., J. C. S.**

Journal Club 3 Research Report Due - Tues Oct. 11th - Guidelines in GDocs folder Virtual Screening help session for setting up your protein (should have started your second library this week)
 * Week 7 (10/10 – 10/14) **
 * Monday 4 - 5:30 in WEL 2.306 Computer Lab.

Express and Purify and Characterize your protein PyMol Image contest Due Friday at Midnight Wikispaces check on each Friday

FPLC purify enzyme, store enzyme Test your enzyme for activity Wikispaces check on each Friday
 * Week 8 (10/17 – 10/21) **
 * VIRTUAL SCREENING - should have 3 libraries completed, at the least **

Journal Club 4 FPLC purify enzyme, store enzyme Test your enzyme for activity or Sat night if working on Sat. Wikispaces check on each Friday
 * Week 9 (10/24 – 10/28) **
 * VIRTUAL SCREENING ** - finish by this week - do **Top Ligands Procotol** to get final compounds for ordering
 * Fri ( 10/28) Lab Notebook check this Friday**(7:00 pm)

=Protein Expression Scheduling:= Here's the Sign Up Form on Google - new guys and returning Spring VDSers should fill this out by Monday night.

Tues Nov. 1s t Thursday Nov 3rd at Midnight (Materials & Methods for **Cloning** due) FPLC purify enzyme, store enzyme Test your enzyme for activity Wikispaces check on each Friday
 * Week 10 (10/31 – 11/4) **
 * FRI External Open House - **Wednesday Nov. 2nd 1- 4 pm
 * Top Ligands Procotol ** to get final compounds for ordering

Journal Club 5 Inhibition Assays Tues Nov. 8th at Midnight (Materials & Methods for **Virtual Screening**due) - see Tips & Hints page Wikispaces check on each Friday
 * Week 11 (11/7 – 11/11) **

Tues Nov. 15th at Midnight (Materials & Methods for **Expression, Purification, and Characterization** due) Purify and Characterize your protein - NOTE: this is a long and arduous process. Don't put it off. Inhibition Assays or Sat night if working on Sat. Wikispaces check on each Friday
 * Week 12 (11/14 – 11/18) **
 * Fri ( 11/18) Lab Notebook check this Friday**(7:00 pm)

Protein Protocols to be completed - also see instructional videos on GDocs
ProtocolProteinExpression_VDS_Fall11_ver2.doc ProtocolProteinPurificationVDS_Fall11.doc ProtocolProteinCharacterizationVDS_Fall11.doc ProtocolFPLC_GelFiltration_VDS_Fall11V_v2.docx

ProtocolLabEnzymeTest_Phosphatase_VDS_Fall11.xlsx Then on to Inhibition Assays once compounds arrive

Journal Club 6 Inhibition Assays - on 2 compounds
 * <span style="font-family: 'Times New Roman','serif';">Week 13 (11/21 – 11/25): Thanksgiving **

Inhibition Assays - on 2 compounds Intro to ICM? Tues Nov. 29th - __FINAL PRESENTATIONS of Enzyme Inhibition Graphs - everyone__ make a one slide PowerPoint and upload to the GroupMeetingSlides/FInalSlides_StudentsFall11 folder on GDocs save file with your name and date in the file name
 * <span style="font-family: 'Times New Roman','serif';">Week 14 (11/28 – 12/2) - Last Class week **


 * Thurs (12/1)** - Lab Cleanup (Required) - each students spends 1 hr cleaning in lab (counts as 1 hour of lab time)
 * Fri (12/2) -** Lab Cleanup (Required) - each students spends 1 hr cleaning in lab (counts as 1 hour of lab time)

Final Wikispaces check
 * Sat ( 12/3)**
 * Final Report due (5:00 pm )**
 * Final Lab Notebook check** (7:00 pm)