Dihydropteroate+synthase+2+Mycobacterium+tuberculosis

**Organism:** Mycobacterium Tuberculosis
 * Target (protein/gene name): ** Dihydropteroate synthase 2
 * E.C Number: ** 2.5.1.15
 * NCBI Gene # or RefSeq#: **NC_000962.3
 * PBD ID: **2VP8
 * Etiologic Risk Group: **N/A
 * Disease Information: **Mycobacterium is an organism that induces the disease Tuberculosis. Mycobacterium does this through the formation of aerosol droplets onto lung-alveolar spaces in the body. The disease was it's first sighting in dirt found on earth but was soon found in animal, specifically cows, which in turn passed on what was called M.bovis (M.tuberculosis) to humans. It was found that over the years the disease has been quite fatal and it wasn't until the 20th century that vaccines and antibiotics were discovered to treat this disease. Although there are discovered treatments for this disease, there is still fatal cases around the world today. The most commons symptoms seen affect the lungs and nervous system.

Links: 1. [|What is Tuberculosis?] 2. [|Mycobacterium tuberculosis Pathogenesis and Molecular Determinants of Virulence]

Dihydropteroate synthase participates in the biosynthetic pathways for folate (in bacteria, plants and fungi) and methanopterin (in archaea). The enzyme exists in varying types of multifunctional proteins in different organisms such as the plant Arabidopsis thaliana and the yeast Saccharomyces cerevisiae.
 * Essentiality: **

[|Expasy Page]
 * Link: **

[|A Promising Target for Novel Anti-Malarial Chemotherapy]
 * Druggable Target: **

[]
 * Assayable Enzyme Target: **

A simple method to detect plasmodium falciparum single nucleotide polymorphisms in many proteins including Dihydropteroate synthase using polymerase chain reaction and enzyme linked immunosorbent assay-based technology.


 * Brenda: **
 * [] **


 * Structure: **

code Query 1    MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADVIDLGPASSNPDAAPVS  60 MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADVIDLGPASSNPDAAPVS Sbjct 13   MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADVIDLGPASSNPDAAPVS  72
 * Pairwise alignment of BlastP: **

Query 61   SDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAYLNDIRGFPDAAFYPQLAK  120 SDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAYLNDIRGFPDAAFYPQLAK Sbjct 73   SDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAYLNDIRGFPDAAFYPQLAK  132

Query 121  SSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAALTGAGIKRNRLVLDPGMGFF  180 SSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAALTGAGIKRNRLVLDPGMGFF Sbjct 133  SSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAALTGAGIKRNRLVLDPGMGFF  192

Query 181  LGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRALTGRGPGDVGAATLAAELAAAA  240 LGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRALTGRGPGDVGAATLAAELAAAA Sbjct 193  LGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRALTGRGPGDVGAATLAAELAAAA  252

Query 241  GGADFIRTHEPRPLRDGLAVLAALKETARIR  271 GGADFIRTHEPRPLRDGLAVLAALKETARIR Sbjct 253  GGADFIRTHEPRPLRDGLAVLAALKETARIR  283

code
 * Query Coverage: ** 100%
 * Max % Identities: ** 100%
 * % Positives: ** 100%
 * Chain used for Homology: ** A


 * Similarity to Human: **No similarity to human was found

In bacteria, antibacterial sulfonamides act as competitive inhibitors of the enzyme dihydropteroate synthase, DHPS. DHPS catalyses the conversion of PABA (para-aminobenzoate) to dihydropteroate, a key step in folate synthesis.
 * Inhibitors:**

[|Folate Inhibitors]

The genes for dihydropteroate synthase of Mycobacterium tuberculosis were expressed under control of the T5 promoter in a dihydropteroate synthase-deficient strain of Escherichia coli. Using three chromatography steps, M. tuberculosis can be purified.
 * Expression: **

[]

There are 4 Transmembrane Bound regions
 * Transmembrane Bound Regions: **


 * TMpred graph Image **

[]
 * TMpred page Link:**

code >NC_000962.3:1351191-1352147 Mycobacterium tuberculosis H37Rv, complete genome GTGCGTTCAACACCGCCGGCCTCAGCCGGTCGTTCAACACCGCCGGCGTTAGCCGGCCATTCAACACCGC CGGCGTTAGCCGGCCATTCAACGCTGTGCGGCCGTCCAGTCGCAGGTGATCGTGCGCTGATCATGGCGAT CGTCAACCGCACCCCGGATTCGTTTTACGACAAGGGTGCGACTTTCAGCGACGCGGCTGCCAGAGACGCG GTCCACCGGGCCGTCGCCGACGGTGCCGACGTCATCGACGTCGGCGGTGTCAAAGCCGGCCCGGGTGAAC GCGTCGACGTCGACACCGAGATCACGCGGCTGGTGCCGTTCATCGAATGGCTCCGCGGTGCTTACCCGGA CCAGCTGATCAGTGTCGACACCTGGCGCGCGCAGGTGGCGAAGGCGGCCTGCGCGGCGGGGGCGGACCTG ATCAACGACACCTGGGGTGGCGTCGACCCGGCCATGCCCGAGGTGGCCGCCGAGTTCGGCGCGGGCCTGG TGTGTGCGCACACCGGCGGCGCGCTGCCACGCACGCGACCCTTCCGGGTGAGCTACGGTACGACTACCCG CGGTGTGGTGGATGCTGTGATTAGCCAGGTCACAGCCGCCGCCGAGCGGGCCGTCGCGGCCGGGGTGGCC CGCGAGAAGGTGTTGATCGACCCGGCACACGACTTCGGCAAGAACACCTTCCATGGGCTGCTGCTATTGC GACACGTGGCCGATCTTGTTATGACCGGGTGGCCCGTGCTGATGGCTTTGAGCAACAAGGACGTTGTCGG GGAGACTCTGGGCGTGGATTTGACCGAACGGCTTGAGGGAACGCTGGCAGCCACCGCGTTGGCTGCGGCC GCCGGGGCGCGCATGTTTCGGGTGCATGAGGTCGCCGCCACCCGGCGGGTGCTGGAAATGGTGGCATCGA TTCAGGGGGTCCGGCCGCCGACGCGCACGGTGAGAGGACTCGCATGA code
 * CDS Gene Sequence:**

code >NC_000962.3:1351191-1352147 Mycobacterium tuberculosis H37Rv, complete genome GTGCGTTCAACACCGCCGGCCTCAGCCGGTCGTTCAACACCGCCGGCGTTAGCCGGCCATTCAACACCGC CGGCGTTAGCCGGCCATTCAACGCTGTGCGGCCGTCCAGTCGCAGGTGATCGTGCGCTGATCATGGCGAT CGTCAACCGCACCCCGGATTCGTTTTACGACAAGGGTGCGACTTTCAGCGACGCGGCTGCCAGAGACGCG GTCCACCGGGCCGTCGCCGACGGTGCCGACGTCATCGACGTCGGCGGTGTCAAAGCCGGCCCGGGTGAAC GCGTCGACGTCGACACCGAGATCACGCGGCTGGTGCCGTTCATCGAATGGCTCCGCGGTGCTTACCCGGA CCAGCTGATCAGTGTCGACACCTGGCGCGCGCAGGTGGCGAAGGCGGCCTGCGCGGCGGGGGCGGACCTG ATCAACGACACCTGGGGTGGCGTCGACCCGGCCATGCCCGAGGTGGCCGCCGAGTTCGGCGCGGGCCTGG TGTGTGCGCACACCGGCGGCGCGCTGCCACGCACGCGACCCTTCCGGGTGAGCTACGGTACGACTACCCG CGGTGTGGTGGATGCTGTGATTAGCCAGGTCACAGCCGCCGCCGAGCGGGCCGTCGCGGCCGGGGTGGCC CGCGAGAAGGTGTTGATCGACCCGGCACACGACTTCGGCAAGAACACCTTCCATGGGCTGCTGCTATTGC GACACGTGGCCGATCTTGTTATGACCGGGTGGCCCGTGCTGATGGCTTTGAGCAACAAGGACGTTGTCGG GGAGACTCTGGGCGTGGATTTGACCGAACGGCTTGAGGGAACGCTGGCAGCCACCGCGTTGGCTGCGGCC GCCGGGGCGCGCATGTTTCGGGTGCATGAGGTCGCCGCCACCCGGCGGGTGCTGGAAATGGTGGCATCGA TTCAGGGGGTCCGGCCGCCGACGCGCACGGTGAGAGGACTCGCATGA code
 * Protein Sequence:**

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)