TargetSp17+-+NADH+ubiquinone+reductase+Na+ion+transporting+(Vibrio+cholerae)


 * Target (protein/gene name): ** NADH:ubiquinone reductase (Na+-transporting)

**NCBI #:** NC_005139.1


 * Protein ID (NP or XP #) or Wolbachia#: ** 331633 (from NCBI)

**Organism (including strain):** // Vibrio cholerae //

**Etiologic Risk Group (see link below):** Group 2

[]

**Disease Information** **(sort of like the Intro to your Mini****Research Write****up):**

// Vibrio cholerae // causes acute diarrhea by infecting human intestines and causes severe dehydration. The bacteria can thrive in human bodies and polluted water [1]. Even though the disease is rare in the United States, there is an increase in the number of cases around the world. Currently there are about 5 million cases and over 100,000 mortalities each year globally. Infection does not necessarily cause symptoms to appear. However, about 10% of the infected people will experience rapid loss of body fluids, electrolytes, and nutrients. Death can happen within several hours if treatment is not given. The disease is mostly diagnosed by stool sample. Cholera is a //reportable// disease in the United States and all samples are sent to state labs for subtyping. Current treatments include rehydration therapy, antibiotic treatment, and zinc treatment. However, antibiotic resistance has appeared in //V. cholerae// due to wide spread use of antibiotics [2]. [] [1] [][2]

**Link to TDR Targets page (if present):** n/a
 * Link to Gene Database page NCBI: ** []

**Previous studies:** ** There are 162 studies on quinone reductase **
 * Essentiality of this protein: **

NADH: ubiquinone oxidoreductase (Na ion pumping) serves as the main ion pump for the electrons entering the respiratory chain of a wide variety of disease causing bacteria. The protein works by creating a sodium gradient across membrane to maintain homeostasis, nutrition cotransport, ATP synthesis, flagella movements, and other vital functions[3]. The protein has also been found in //Chlamydiophyla pneumoniae// to help increase adaptive potential by maintaining internal homeostasis in environments with changing salinity and pH. This target can be inhibited to severely disrupt bacteria internal balance, thus impacting its ability to infect and adapt in the human body[4].

[][3] [][4]

**Is it a monomer or multimer as biological unit:** Monomer in vibrio cholerae.

**Complex of proteins?:** Several subunits.

**Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):** **[]**

***EC#:** 1.6.5.8


 * Link to BRENDA EC# page: ** []

**Enzyme Assay** **information:** ** Not specifically for 1.6.5.8, but there are many assay protocol for many different reductases on Sigma. There are assay protocols for the enzyme //Vibrio cholerae//. ** []
 * -- links to assay reagents (substrates) pages. **
 * [] **

**--- List cost and quantity of substrate reagents, supplier, and catalog #**

n/a

**Structure (PDB or Homology model)**

**Similarity to Human:** highest similarity 36.7% with OR51B6

**Current Inhibitors:** There are currently several inhibitors that are weak and have low binding affinity

**Expression Information (has it been expressed in bacterial cells):** yes

** Purification Method ** ** : ** D-salt polyacrylamide 6000 gel filtration []


 * Image of protein (PyMol with features delineated and shown separately): **

Figure 1. 3D image of NADH: ubiquinone reductase (Na+ transporting) of Vibrio cholerae from PDB, shown in cartoon in PyMol and colored by chain.

**Amino Acid Sequence:**
>tr|A0A177NCL7|A0A177NCL7_9GAMM Na(+)-translocating NADH-quinone reductase subunit E OS=Methylomonas koyamae GN=nqrE PE=3 SV=1

MEAYIALFVKAVFIENLALSFFLGMCTFLAVSKKIATAMGLGVAVMVVQTLTVPANNFIY

QTLLKEDALSWLGITGVDLSFLSLLSCIGMIAAIVQILEMFLDKFVPALYNALGIFLPLI

TVNCAILAGSLFMIERDYNFGESVVYGIGSGFGWALAITLMAGVREKLKYSDVPAALQGL

GITFITAGLMSLGFMAFSGIQL

[]

** Length of your protein in Amino Acids: ** 202
** Molecular Weight ** ** of your protein in kiloDaltons: ** 27600 kDaltons


 * Molar ** ** Extinction coefficient ** ** of your protein at 280 nm wavelength: ** 20065 (  []  )


 * TMpred graph Image ** ( @http://www.ch.embnet.org/software/TMPRED_form.html  ).

Figure 2. TMpred graph of Na(+) translocating NADH-quinone reductase subunit E from Methylomonas koyamae.

>NC_005139.1:c2614943-2613720 Vibrio vulnificus YJ016 DNA, chromosome I, complete sequence code ATGGACATTATTCTTGGTGTAGTGATGTTTACTCTGATCGTACTGGCTCTAGTACTAGTGATTCTTTTCG code code CTAAGTCTAAGCTTGTACCAACAGGTGACATTACAATTTCTATCAACGGCGACGCAGATAAGTCGATCGT code code TACTTCTCCAGGTGGCAAGCTACTAAGCGCGTTAGCTGGTGCAGGTGTATTCGTTTCTTCTGCTTGTGGT code code GGCGGTGGCTCTTGTGGTCAGTGTCGCGTAAAAGTGAAGAGCGGTGGTGGTGATATCCTTCCAACTGAGT code code TGGATCACATCACTAAAGGTGAAGCGCGTGAAGGTGAGCGTCTAGCGTGTCAGGTGGCTGTGAAAACAGA code code CATGGACATCGAGCTACCAGAAGAAATCTTCGGCGTTAAAAAGTGGGAATGTACGGTTATCTCTAACGAT code code AACAAAGCAACATTCATTAAAGAGCTTAAGCTACAAATTCCAGATGGCGAATCAGTACCTTTCCGTGCTG code code GTGGTTACATCCAGATTGAAGCACCAGCTCACCACGTTAAATACGCAGATTTCGACGTACCAGAAGAGTA code code CCGCGGTGACTGGGACAAGTTTAACTTGTTCCGTTACGAGTCTATCGTAAAAGAAGACATCATCCGTGCA code code TACTCAATGGCGAACTACCCTGAAGAGTTCGGCATCATCATGCTAAACGTACGTATCGCGACTCCGCCGC code code CAAATAACCCAGACGTAGCACCGGGTCAGATGTCTTCTTACATCTGGTCACTAAAAGAAGGTGATAAGTG code code TACGATTTCTGGTCCATTTGGTGAGTTCTTCGCAAAAGATACAGATGCAGAAATGGTCTTCATCGGTGGT code code GGTGCAGGTATGGCTCCTATGCGTTCTCATATCTTTGACCAACTGAAGCGTTTGAAGTCTAAGCGTAAGA code code TGTCTTACTGGTACGGCGCGCGTTCTAAGCGTGAAATGTTCTACGTAGAAGATTTCGATGGCCTAGCGGC code code TGAGAATGATAACTTCGTATGGCACTGTGCACTGTCTGATCCACTCCCAGAAGATAACTGGGATGGTTAC code code ACAGGCTTCATCCACAATGTGCTTTACGAAAACTATCTGCGTGATCACGATGCACCAGAAGATTGTGAAT code code ACTACATGTGTGGTCCACCGATGATGAACGCGGCTGTTATCGGCATGCTGAAAAATCTCGGTGTAGAAGA TGAAAACATCCTACTGGATGACTTCGGTGGTTAA code
 * *CDS ** ** Gene Sequence ** ** (paste as text only): **


 * *GC% Content for gene: **** 46.16% **
 * *GC% Content for gene (codon optimized): **** n/a **