-+UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopi+melate-D-alanyl-D-alanyl+ligase+(Mycobacterium+tuberculosis)

out in the air that can infect the surface in the lungs which secretes pulmonary surfactant that decreases the surface tension of the alveoli. The most common symptoms in a Tuberculosis-infected patient are coughing, fatigue, a high fever, and chest pain. Those who have poor nutritional diets, live in places that do not offer medical care, or are highly populated risk getting Tuberculosis. Because of its resiliency tendency, M.tuberculosis bacterium undergo quick mutations resulting in more resistant strains compared to other bacteria, so it is important to keep up with the strains and research in order to prevent the spread of the disease.
 * Target (protein/gene name): ** UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopi melate-D-alanyl-D-alanyl ligase ( [|Rv2158c] )
 * NCBI Gene # or RefSeq#: ** NC_000962.3
 * Protein ID (NP or XP #) or Wolbachia#: **[|NP_216674.1]
 * Organism (including strain): ** Mycobacterium tuberculosis (H37Rv)
 * Etiologic Risk Group (see link below): ** N/A
 * Disease Information (sort of like the Intro to your Mini Research Write up): ** Mycobacterium tuberculosis is a pathogenic-type bacteria that is in charge of the infectious Tuberculosis disease. Tuberculosis is highly infective, known for having a high morbidity and a high mortality rate. The disease starting infecting more and becoming more severe after 1985 when HIV entered into body systems and lowered the immunity of the infected. The severity of the bacteria is contagious by its microscopic bodily fluids

http://www.tdrtargets.org/targets/view?gene_id=6551 [|https://www.patricbrc.org/view/Feature/PATRIC.1643430.3.LDXW01000001.CDS.103333.104823.rev] []
 * Link to TDR Targets page (if present): **
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) **

Rv2158c has essentiality data
 * Essentiality of this protein: **

Gene/Ortholog: eco86 (OG4_14754); Phenotype: undefined; Source study: blattner

Gene/Ortholog: eco86 (OG4_14754); Phenotype: essential; Source study: gerdes

Gene/Ortholog: eco86 (OG4_14754); Phenotype: essential; Source study: keio

Gene/Ortholog: eco86 (OG4_14754); Phenotype: essential; Source study: shigen

No it is not.
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **

[|**6.3.2.13**]
 * EC#: **

@http://www.brenda-enzymes.org/enzyme.php?ecno=6.3.2.13&UniProtAcc=P9WJL3&OrganismID=3445
 * Link to BRENDA EC# page: **

[|http://www.brenda-enzymes.org/pathway_index.php?menu=off&site=pathway&search_mode=exact&pathway=peptidoglycan_biosynthesis&ecno=6.3.2.13]
 * Link to BRENDA HPTR Pathway: **

colorimetric phosphate detection method [1] @https://www.researchgate.net/publication/45707755_Anti-tubercular_screening_of_natural_product_from_Colombian_plants_3-methoxynordomesticine_an_inhibitor_of_MurE_ligase_of_Mycobacterium_tuberculosis [2] []
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **

Fluorogenic substrates, DMSO and PBS DMSO - $68.50 for 100 mL PBS - $110 for 1L

PDB # or closest PDB entry if using homology model: 2WTZ []
 * Structure (PDB or Homology model) **

Nine compounds for the Target in M. tuberculosis
 * Current Inhibitors: **

[1] Chandrakala Basavannacharya, Giles Robertson, Tulika Munshi, Nicholas H. Keep, Sanjib Bhakta Tuberculosis (Edinb) 2010 Jan; 90(1): 16–24. Published online 2009 Nov 27. doi: 10.1016/j.tube.2009.10.007 ATP-dependent MurE ligase in Mycobacterium tuberculosis: biochemical and structural characterisation. [2] Basavannacharya C, Moody PR, Munshi T, Cronin N, Keep NH, Bhakta S. Essential residues for the enzyme activity of ATP-dependent MurE ligase from //Mycobacterium tuberculosis //. //Protein & Cell //. 2010;1(11):1011-1022. doi:10.1007/s13238-010-0132-9.
 * Expression Information (has it been expressed in bacterial cells): **


 * Image of protein (PyMol with features deline ated and shown separately):**
 * Fig 1. Crystal structure of Mycobacterium tuberculosis UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopi melate-D-alanyl-D-alanyl ligase compared with the 2WTZ MurE ligase**

>2WTZ:A|PDBID|CHAIN|SEQUENCE MSSLARGISRRRTEVATQVEAAPTGLRPNAVVGVRLAALADQVGAALAEGPAQRAVTEDRTVTGVTLRAQDVSPGDLFAA LTGSTTHGARHVGDAIARGAVAVLTDPAGVAEIAGRAAVPVLVHPAPRGVLGGLAATVYGHPSERLTVIGITGTSGKTTT TYLVEAGLRAAGRVAGLIGTIGIRVGGADLPSALTTPEAPTLQAMLAAMVERGVDTVVMEVSSHALALGRVDGTRFAVGA FTNLSRDHLDFHPSMADYFEAKASLFDPDSALRARTAVVCIDDDAGRAMAARAADAITVSAADRPAHWRATDVAPTDAGG QQFTAIDPAGVGHHIGIRLPGRYNVANCLVALAILDTVGVSPEQAVPGLREIRVPGRLEQIDRGQGFLALVDYAHKPEAL RSVLTTLAHPDRRLAVVFGAGGDRDPGKRAPMGRIAAQLADLVVVTDDNPRDEDPTAIRREILAGAAEVGGDAQVVEIAD RRDAIRHAVAWARPGDVVLIAGKGHETGQRGGGRVRPFDDRVELAAALEALERRA
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **

535 AA 55.34076 kDa 18575
 * length of your protein in Amino Acids**
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website**
 * Molar Extinction efficient of your protein at 280 nm wavelenth:**

Input your amino acid sequence to it.
 * TMpred graph Image [|TMPRED]**

code >>Mycobacterium tuberculosis H37Rv|CDS|Rv2158c|2419001-2420608|-|murE|downstream:0|upstream:0 gtgtcatcgctggcccgagggatctcgcggcggcgaacggaggtggcgacacaggtggaggctgcgcccactg gcttgcgccccaacgccgtcgtgggcgttcggttggccgcactggccgatcaggtcggcgcggccctggccga gggtccagctcagcgtgccgtcaccgaggaccggacggtcaccggggtcacgctgcgcgcccaggacgtgtca cccggtgacctgttcgccgccctgaccggctcgaccacccacggggcccgccacgtcggcgacgcgatcgcac gcggcgccgtcgcggtgctcaccgaccccgccggggtcgccgagatcgccggacgagcggccgtgcccgtgtt ggtgcaccccgcaccccgcggcgtgctcggcggcttggccgccaccgtgtacgggcatccgtccgagcggttg acggttatcgggatcaccggaacgtccggcaagaccaccaccacctatctggtcgaggccgggttacgggctg ccggacgcgtcgccgggctgatcggcaccatcggcatccgcgtcggcggcgccgaccttcccagcgcgctgac caccccggaggcccccacgctgcaggcgatgctggcggcgatggtcgaacgcggggtggacaccgtggtcatg gaggtgtccagccacgcgctggcgctgggccgggtggacggcacccggttcgccgtcggcgccttcaccaatc tctcccgtgaccacctggatttccaccccagcatggccgactacttcgaggccaaggcgtcattgttcgatcc ggactcggcactgcgcgcccgcaccgccgtggtgtgcatcgacgacgacgccgggcgcgcgatggcggcgcgg gccgccgacgcgatcaccgtcagcgccgccgaccggcccgcacactggcgcgccacggatgtggcgcccacgg acgcgggcgggcaacaattcaccgccatcgaccccgccggcgtagggcatcacatcggaatccggctaccggg ccgctacaacgtcgccaattgcctggtcgccctggcgattctggacaccgtcggggtctccccggaacaggcg gtgccgggcctgcgtgagatccgggtcccggggcggctcgagcagatcgaccgcggccagggctttctcgcgc tggtcgactacgcgcacaaaccggaagcgctgcggtcggtgctgaccaccttggcgcacccggaccgccggct ggcggtggtgttcggcgccggcggcgatcgtgacccgggcaagcgggccccgatgggccggatagccgcgcag ctggccgacttggtggtcgtcaccgacgacaacccgcgtgacgaagatcccacggcgatccgccgcgaaatcc tggctggggcggccgaagtcggcggtgatgcccaggtcgtcgagatcgcagaccggcgggacgcgatccggca cgcggttgcctgggcgcgccccggcgacgtggtgctcatcgccggcaaaggccacgagaccgggcaacgcggc ggcgggcgggtccgcccgttcgacgaccgggtggagctggctgccgcgctagaggccctcgagcggcgcgcatga code code format="sequence" >>Mycobacterium tuberculosis H37Rv|Rv2158c|murE 10        20         30         40         50 MSSLARGISR RRTEVATQVE AAPTGLRPNA VVGVRLAALA DQVGAALAEG 60        70         80         90        100 PAQRAVTEDR TVTGVTLRAQ DVSPGDLFAA LTGSTTHGAR HVGDAIARGA 110       120        130        140        150 VAVLTDPAGV AEIAGRAAVP VLVHPAPRGV LGGLAATVYG HPSERLTVIG 160       170        180        190        200 ITGTSGKTTT TYLVEAGLRA AGRVAGLIGT IGIRVGGADL PSALTTPEAP 210       220        230        240        250 TLQAMLAAMV ERGVDTVVME VSSHALALGR VDGTRFAVGA FTNLSRDHLD 260       270        280        290        300 FHPSMADYFE AKASLFDPDS ALRARTAVVC IDDDAGRAMA ARAADAITVS 310       320        330        340        350 AADRPAHWRA TDVAPTDAGG QQFTAIDPAG VGHHIGIRLP GRYNVANCLV 360       370        380        390        400 ALAILDTVGV SPEQAVPGLR EIRVPGRLEQ IDRGQGFLAL VDYAHKPEAL 410       420        430        440        450 RSVLTTLAHP DRRLAVVFGA GGDRDPGKRA PMGRIAAQLA DLVVVTDDNP 460       470        480        490        500 RDEDPTAIRR EILAGAAEVG GDAQVVEIAD RRDAIRHAVA WARPGDVVLI 510       520        530 AGKGHETGQR GGGRVRPFDD RVELAAALEA LERRA code code Do Not Need this info for Spring (but still copy these lines to your Target page for now Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers link to DNA Works output m9l40l772 that should be saved in your folder after you did the primer design protocol -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not< sure how to. Primer design results for 'tail' primers (this is just 2 sequences):
 * Fig. 2 TMpred output for UDP-N-acetylmuramoylalanyl-D-glutamyl-2,** **6-diaminopi melate-D-alanyl-D-alanyl ligase**
 * Gene Sequence (paste as text only):**
 * Protein Sequence (paste as text only):**

code