ResearchPage+-+Giancarlo

Giancarlo's Research
Giancarlo - put these in reverse chronological order. - Dr. B

Week 1: First Day 1) Prepare Template Dilutions for PCR 2) Run Dilutions though PCR machine (overnight). Week 1: Second Day 3) Create Agarose Gel 4) Run Gel for 40 minutes
 * Intoduction to PCR Protocol**

Figure 1: PCR-1 Gel Run: This gel run was done incorrectly due to the fact that only two lanes are visible. One needs five lanes visible for it to be done correctly.

Week 2: (PCR 1 didn't work) -First Day -Enzyme Digest -Redid PCR-1 -prepare template dilutions for PCR -run dilutions through PCR machine. -Second Day -PCR-1 -create Agarose gel -run PCR dilutions through PCR gel for 40 minutes -take picture of finished gel Figure 2: Week 3: Day1: -PCR-2 -create plasmid dilutions -create mater mix -create templates -add TAQ polymerase to each template -run templates through PCR machine -Enzyme Digest Gel Run Figure 3: (Enzyme Digest) Day 2: -run PCR-2 templates through gel -take picture Figure 4: (PCR2) (Failed)

This gel sample failed because only the DNA ladder appeared. If done correctly, the DNA ladder and the rest of the 4 samples would have appeared.

Week 4: Day 1: -attempted to do VirtualRefresher, but computer froze and runs won't run.

Day 2: -Since PCR 2 failed the first time, I had to do it again. -Run samples through PCR machine -Make gel -Run samples through gel Figure 5: (PCR2)(Failed AGAIN!)

Week 6: Day 1: -started primer overlap -attempted to do Virtual Refresher, but only the 4th and 5th folder didn't run (check again tomorrow)

Week 7: (during week 6 I attended lab for only 6 hours, so I'm continuing Virtual Refresher and Primer overlap) -Secondary PCR for Primer Overlap -Gel Run for Primary and Secondary PCR's -I ran the 4th and 5th folders for Virtual Refresher -Gel run for Primary and Secondary PCR (Figure below: FAILED)

Week 8: -Since Primary and Secondary PCR gel run FAILED, I had to redo the whole primer overlap lab.