Target+-+Succinate+Dehydrogenase+(Flavoprotein+subunit)+SDHA+(Succinic+dehydrogenase)+(Fumarate+reductase)+(Fumarate+dehydrogena)

http://www.absa.org/riskgroups/bacteriasearch.php?genus=Mycobacterium&species=tuberculosis
 * *<span style="background-color: transparent !important; background-position: initial initial !important; background-repeat: initial initial !important; border: none !important; display: inline-block !important; float: none !important; font-family: arial,helvetica,sans-serif !important; font-size: 13.333333015441895px !important; font-style: normal !important; font-variant: normal !important; font-weight: bold !important; height: auto !important; line-height: 26px !important; margin: 0px !important; min-height: 0px !important; min-width: 0px !important; padding: 0px !important; text-decoration: underline !important; vertical-align: baseline !important; width: auto !important;">[[image:http://cdncache1-a.akamaihd.net/items/it/img/arrow-10x10.png height="26"]] (protein/gene name): **SUCCINATE DEHYDROGENASE FLAVOPROTEIN SUBUNIT [Mycobacterium tuberculosis]
 * *NCBI Gene # or RefSeq#: ** 532538596
 * *Protein ID (NP or XP #) or Wolbachia#: ** Rv3318
 * *Organism (including strain): ** Mycobacterium tuberculosis
 * Etiologic Risk Group (see link below): ** 3


 * *Backgound [[image:http://cdncache1-a.akamaihd.net/items/it/img/arrow-10x10.png link="vdsstream/Target- PHOSPHOTYROSINE PROTEIN PHOSPHATASE PTPB (Mycobacterium tuberculosis)"]] /Disease Information (sort of like the Intro to your Mini Research Up): ** Mycobacterium tuberculosis (MTB) is a pathogenic bacterial species in the family Mycobacteriaceae and the causative agent of most cases of tuberculosis. Robert Koch first discovered M. tuberculosis in 1882, revealing that MTB has an unusual, waxy coating on its cell surface which makes the cells impervious to Gram staining, therefore acid-fast detection techniques are used instead. The physiology of M. tuberculosis is highly aerobic and requires high levels of oxygen. MTB, primarily a pathogen of the mammalian respiratory system, infects the lungs. Today, the most frequently used diagnostic methods for TB are the tuberculin skin test, acid-fast stain, and chest radiographs.


 * Link to TDR Targets page (if present): ** http://tdrtargets.org/targets/view?gene_id=9874


 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.): **http://www.ncbi.nlm.nih.gov/protein/EQM22493.1

Rv3318 has essentiality data Gene/Ortholog: mtu3378 (OG4_10363); Phenotype: non-essential; Source study: nmpdr Gene/Ortholog: eco701 (OG4_10363); Phenotype: undefined; Source study: blattner Gene/Ortholog: eco701 (OG4_10363); Phenotype: non-essential; Source study: gerdes Gene/Ortholog: eco701 (OG4_10363); Phenotype: non-essential; Source study: keio Gene/Ortholog: eco701 (OG4_10363); Phenotype: non-essential; Source study: shigen Gene/Ortholog: eco4000 (OG4_10363); Phenotype: non-essential; Source study: blattner Gene/Ortholog: eco4000 (OG4_10363); Phenotype: non-essential; Source study: gerdes Gene/Ortholog: eco4000 (OG4_10363); Phenotype: non-essential; Source study: keio Gene/Ortholog: eco4000 (OG4_10363); Phenotype: non-essential; Source study: shigen Gene/Ortholog: cel3359 (OG4_10363); Phenotype: Embryonic Lethal/<span style="background-color: transparent !important; background-position: initial initial !important; background-repeat: initial initial !important; border: none !important; display: inline-block !important; float: none !important; font-family: arial,helvetica,sans-serif !important; font-size: 13.333333015441895px !important; font-style: normal !important; font-variant: normal !important; font-weight: normal !important; height: auto !important; line-height: 26px !important; margin: 0px !important; min-height: 0px !important; min-width: 0px !important; padding: 0px !important; text-decoration: underline !important; vertical-align: baseline !important; width: auto !important;"> ; Source study: neb Gene/Ortholog: cel3359 (OG4_10363); Phenotype: Larval/Adult Lethal/<span style="background-color: transparent !important; background-position: initial initial !important; background-repeat: initial initial !important; border: none !important; display: inline-block !important; float: none !important; font-family: arial,helvetica,sans-serif !important; font-size: 13.333333015441895px !important; font-style: normal !important; font-variant: normal !important; font-weight: normal !important; height: auto !important; line-height: 26px !important; margin: 0px !important; min-height: 0px !important; min-width: 0px !important; padding: 0px !important; text-decoration: underline !important; vertical-align: baseline !important; width: auto !important;"> ; Source study: neb Gene/Ortholog: cel940 (OG4_10363); Phenotype: Growth Defect; Source study: neb Gene/Ortholog: cel940 (OG4_10363); Phenotype: Embryonic Lethal/<span style="background-color: transparent !important; background-position: initial initial !important; background-repeat: initial initial !important; border: none !important; display: inline-block !important; float: none !important; font-family: arial,helvetica,sans-serif !important; font-size: 13.333333015441895px !important; font-style: normal !important; font-variant: normal !important; font-weight: normal !important; height: auto !important; line-height: 26px !important; margin: 0px !important; min-height: 0px !important; min-width: 0px !important; padding: 0px !important; text-decoration: underline !important; vertical-align: baseline !important; width: auto !important;"> ; Source study: neb Gene/Ortholog: cel940 (OG4_10363); Phenotype: Morphology Defect; Source study: neb Gene/Ortholog: cel3359 (OG4_10363); Phenotype: Embryonic Lethal/Arrest; Source study: wormbase Gene/Ortholog: cel940 (OG4_10363); Phenotype: Embryonic Lethal/Arrest; Source study: wormbase Gene/Ortholog: Tb927.8.6580 (OG4_10363); Phenotype: significant gain of <span style="background-color: transparent !important; background-position: initial initial !important; background-repeat: initial initial !important; border: none !important; display: inline-block !important; float: none !important; font-family: arial,helvetica,sans-serif !important; font-size: 13.333333015441895px !important; font-style: normal !important; font-variant: normal !important; font-weight: normal !important; height: auto !important; line-height: 26px !important; margin: 0px !important; min-height: 0px !important; min-width: 0px !important; padding: 0px !important; text-decoration: underline !important; vertical-align: baseline !important; width: auto !important;"> in bloodstream forms (3 days); Source study: alsford Gene/Ortholog: Tb927.8.6580 (OG4_10363); Phenotype: significant gain of <span style="background-color: transparent !important; background-position: initial initial !important; background-repeat: initial initial !important; border: none !important; display: inline-block !important; float: none !important; font-family: arial,helvetica,sans-serif !important; font-size: 13.333333015441895px !important; font-style: normal !important; font-variant: normal !important; font-weight: normal !important; height: auto !important; line-height: 26px !important; margin: 0px !important; min-height: 0px !important; min-width: 0px !important; padding: 0px !important; text-decoration: underline !important; vertical-align: baseline !important; width: auto !important;"> in bloodstream forms (6 days); Source study: alsford Gene/Ortholog: Tb927.8.6580 (OG4_10363); Phenotype: no significant loss or gain of <span style="background-color: transparent !important; background-position: initial initial !important; background-repeat: initial initial !important; border: none !important; display: inline-block !important; float: none !important; font-family: arial,helvetica,sans-serif !important; font-size: 13.333333015441895px !important; font-style: normal !important; font-variant: normal !important; font-weight: normal !important; height: auto !important; line-height: 26px !important; margin: 0px !important; min-height: 0px !important; min-width: 0px !important; padding: 0px !important; text-decoration: underline !important; vertical-align: baseline !important; width: auto !important;"> in procyclic forms; Source study: alsford Gene/Ortholog: Tb927.8.6580 (OG4_10363); Phenotype: significant gain of fitness in differentiation of procyclic to bloodstream forms; Source study: alsford
 * Essentiality of this protein: **


 * Complex of proteins?: ** n/a
 * Druggable <span style="background-color: transparent !important; background-position: initial initial !important; background-repeat: initial initial !important; border: none !important; display: inline-block !important; float: none !important; font-family: arial,helvetica,sans-serif !important; font-size: 13.333333015441895px !important; font-style: normal !important; font-variant: normal !important; font-weight: bold !important; height: auto !important; line-height: 26px !important; margin: 0px !important; min-height: 0px !important; min-width: 0px !important; padding: 0px !important; text-decoration: underline !important; vertical-align: baseline !important; width: auto !important;">[[image:http://cdncache1-a.akamaihd.net/items/it/img/arrow-10x10.png height="26"]] (list number or cite evidence from a paper/database showing druggable in another organism): ** 0.7


 * *EC#: ** 1.3.99.1
 * Link to BRENDA EC# page: **http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.3.99.1




 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **

__ **Catalog Number:** __ SAB1100429 __ **Cost:** __ $317.50 __**Supplier:**__ Sigma-Aldrich
 * Anti-SDHA (131-145) antibody produced in rabbit **

http://www.sigmaaldrich.com/catalog/product/sigma/sab1100429?lang=en®ion=US
 * -- link to Sigma (or other company ) page for assay (see Sigma links below) **

**PDB #:** 2LFC http://www.rcsb.org/pdb/results/results.do?qrid=EEF61829&tabtoshow=Current
 * Structure (PDB or Homology model) **

http://www.bindingdb.org/jsp/dbsearch/PrimarySearch_pubmed.jsp?pubmed=50031969&pubmed_submit=TBD
 * Current Inhibitors: **
 * CHEMBL1170848 **
 * CHEMBL1170849 **
 * CHEMBL1170850 **
 * CHEMBL1170851**


 * Expression Information (has it been expressed in bacterial cells): ** Yes, in E. coli cells.


 * Image of protein: **

code MSTDIPATVSAETVTSWSDDVDVTVIGFGIAGGCAAVSAAAAGARVLVLERAAAAGGTTALAGGHFYLGG GTTVQLATGHPDSPEEMYKYLVAVSREPDHDKIRAYCDGSVEHFNWLEGLGFQFERSYFPGKAVIQPNTE GLMFTGNEKVWPFLELAVPAPRGHKVPVPGDTGGAAMVIDLLLKRAASLGIQIRYETGATELIVDGTGKV TGVMWKRFSETGAIKAKSVIIAAGGFVMNPDMVAKYTPKLAEKPFVLGNTYDDGLGIRLGVSAGGATQHM DQMFITAPPYPPSILLTGIIVNKLGQRFVAEDSYHSRTAGFIMEQPDSAAYLIVDEAHLEHPKMPLVPLI DGWETVVEMEAALGIPPGNLAATLDRYNAYAARGADPDFHKQPEFLAAQDNGPWGAFDMSLGKAMYAGFT LGGLATSVDGQVLRDDGAVVAGLYAVGACASNIAQDGKGYASGTQLGEGSFFGRRAGAHAAARAQGM code
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **

code Ext. coefficient   49975 Abs 0.1% (=1 g/l)  1.126, assuming all pairs of Cys residues form cystines
 * *length of your protein in Amino Acids: ** 417
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website: ** 44.3985 kDa

Ext. coefficient   49850 Abs 0.1% (=1 g/l)  1.123, assuming all Cys residues are reduced code http://web.expasy.org/cgi-bin/protparam/protparam
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.



atgagcaccgatattccggcgaccgtgagcgcggaaaccgtgaccagctggagcgatgat gtggatgtgaccgtgattggctttggcattgcgggcggctgcgcggcggtgagcgcggcg gcggcgggcgcgcgcgtgctggtgctggaacgcgcggcggcggcgggcggcaccaccgcg ctggcgggcggccatttttatctgggcggcggcaccaccgtgcagctggcgaccggccat ccggatagcccggaagaaatgtataaatatctggtggcggtgagccgcgaaccggatcat gataaaattcgcgcgtattgcgatggcagcgtggaacattttaactggctggaaggcctg ggctttcagtttgaacgcagctattttccgggcaaagcggtgattcagccgaacaccgaa ggcctgatgtttaccggcaacgaaaaagtgtggccgtttctggaactggcggtgccggcg ccgcgcggccataaagtgccggtgccgggcgataccggcggcgcggcgatggtgattgat ctgctgctgaaacgcgcggcgagcctgggcattcagattcgctatgaaaccggcgcgacc gaactgattgtggatggcaccggcaaagtgaccggcgtgatgtggaaacgctttagcgaa accggcgcgattaaagcgaaaagcgtgattattgcggcgggcggctttgtgatgaacccg gatatggtggcgaaatataccccgaaactggcggaaaaaccgtttgtgctgggcaacacc tatgatgatggcctgggcattcgcctgggcgtgagcgcgggcggcgcgacccagcatatg gatcagatgtttattaccgcgccgccgtatccgccgagcattctgctgaccggcattatt gtgaacaaactgggccagcgctttgtggcggaagatagctatcatagccgcaccgcgggc tttattatggaacagccggatagcgcggcgtatctgattgtggatgaagcgcatctggaa catccgaaaatgccgctggtgccgctgattgatggctgggaaaccgtggtggaaatggaa gcggcgctgggcattccgccgggcaacctggcggcgaccctggatcgctataacgcgtat gcggcgcgcggcgcggatccggattttcataaacagccggaatttctggcggcgcaggat aacggcccgtggggcgcgtttgatatgagcctgggcaaagcgatgtatgcgggctttacc ctgggcggcctggcgaccagcgtggatggccaggtgctgcgcgatgatggcgcggtggtg gcgggcctgtatgcggtgggcgcgtgcgcgagcaacattgcgcaggatggcaaaggctat gcgagcggcacccagctgggcgaaggcagcttttttggccgccgcgcgggcgcgcatgcg gcggcgcgcgcgcagggcatg
 * *CDS Gene Sequence (paste as text only): **


 * *GC% Content for gene: ** Not <span style="background-color: transparent !important; background-position: initial initial !important; background-repeat: initial initial !important; border: none !important; display: inline-block !important; float: none !important; font-family: arial,helvetica,sans-serif !important; font-size: 13.333333015441895px !important; font-style: normal !important; font-variant: normal !important; font-weight: normal !important; height: auto !important; line-height: 26px !important; margin: 0px !important; min-height: 0px !important; min-width: 0px !important; padding: 0px !important; text-decoration: underline !important; vertical-align: baseline !important; width: auto !important;">[[image:http://cdncache1-a.akamaihd.net/items/it/img/arrow-10x10.png height="26"]].
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): ** Not <span style="background-color: transparent !important; background-position: initial initial !important; background-repeat: initial initial !important; border: none !important; display: inline-block !important; float: none !important; font-family: arial,helvetica,sans-serif !important; font-size: 13.333333015441895px !important; font-style: normal !important; font-variant: normal !important; font-weight: normal !important; height: auto !important; line-height: 26px !important; margin: 0px !important; min-height: 0px !important; min-width: 0px !important; padding: 0px !important; text-decoration: underline !important; vertical-align: baseline !important; width: auto !important;">[[image:http://cdncache1-a.akamaihd.net/items/it/img/arrow-10x10.png height="26"]].
 * *GC% Content for gene (codon optimized): ** Not Available.

Do Not Need this info for Spring (but still copy these lines to your <span style="background-color: transparent !important; background-position: initial initial !important; background-repeat: initial initial !important; border: none !important; display: inline-block !important; float: none !important; font-family: arial,helvetica,sans-serif !important; font-size: 13.333333015441895px !important; font-style: normal !important; font-variant: normal !important; font-weight: normal !important; height: auto !important; line-height: 26px !important; margin: 0px !important; min-height: 0px !important; min-width: 0px !important; padding: 0px !important; text-decoration: underline !important; vertical-align: baseline !important; width: auto !important;"> page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)

**
 * Primer design results for 'tail' primers (this is just 2 sequences): **