Research+Page-Justin

**Justin's Research**

 * Week 9 - Virtual Screening & Protein Expression **


 * Week 8 - Nothing. Too Many Tests... **


 * Week 7 - PP2Ctg Virtual Screening & Lab Report **


 * Week 6 - PP2Ctg Virtual Screening & Protein Expression **


 * Week 5 - Virtual Screening Refresher - looks good - Dr. B. any wet lab data? **

All the top ten ligands determined by gold have long structures of aromatic rings joined by carbon chains, allowing them to position themselves in a bent conformation inside the active site. The cyclic rings seem to contribute to the molecules’ general compatibility with the shape of the active site. The inclusion of a substituent midway through the carbon chain seems to act as a sort of anchoring tail, allowing the molecules to create an additional bond with a residue in the middle of the active site that lies a little further than the others away from the bulk of the molecules’ structures. These purine/pyrimidine-like rings most likely play a large part in the binding of the ligands, which corresponds with the known function of the protein, which includes the manufacturing of molecules later used to create nucleotides. The ability to donate one hydrogen and accept three hydrogens in order to create hydrogen bonds holds true for the top three ligands, possibly leading to the conclusion that the active site has an identical reverse ability to make hydrogen bonds. The three top ligands, according to the Lipinski’s Rule of Five, have promise for medical applications.


 * Week 4 - Version 3 Transformation and Mini-Prep Processing for Sequencing. **






 * Week 3 Nanodrop Deduced Concentrations of CDS for Version 3 and PyMOL Refresher **


 * Week 2 - Primer Overlap PCR and Cutting of Pnic-Bsa4 for Version 3 **


 * Week 1 - Nothing **