TargetSp17+-+S-adenosylmethionine+syntheses+(Coxiella+burnetii)


 * Organism: ** Coxiella burnetii
 * NCBI#: ** NP_005902.1
 * Target: ** S-adenosylmethionine synthetase (EC 2.5.1.6)
 * Protein ID: ** 289455

Q fever is a bacterial disease that is caused by Coxiella brunetti. This disease is more abundant in animals, but Q fever has to potential to be used as biological warfare. It is an airborne that is contacted mainly by being in the environment of an affected animal. The symptoms vary to being flu like or it can be chronic and feel like hepatitis.
 * Disease information: **

S-adenosylmethionine synthetase plays an essential role in metabolism in organisms. It catalyzes synthesis reactions. The formation is from methionine and ATP. The enzyme consists of four subunits and there are two active sites on the protein. Both S-adenosylmethionine and triphosphate are formed with the protein. Link to TDR Targets page (if present): n/a Link to Gene Database page NCBI: []
 * Essentiality of this protein: **

S-adenosylmethionine synthetase is an enzyme in Coxiella burnetii that has four subunits. It synethesises S-adenosylmethionine (AdoMet), pyrophosphate (PPi), and orthophosphate (Pi) from ATP and L-methionine.
 * Complex of protein:**

[] [] EC#: 2.5.1.6
 * Druggable Target ** (list number or cite evidence from a paper/database showing druggable in another organism):


 * Link to BRENDA EC# page ** : []
 * Enzyme Assay information:** []


 * Structure (PDB or Homology model):** 4ODJ


 * Similarity to Humans:** gene sequence isn’t found in BLASTP


 * Current inhibitors:** There are 47 inhibitors


 * Expression information:** Expression of the alpha subunit in E. coli, gel filtration


 * Purification Method:** In humans, there are two methods. The first involved purification of the His-tagged protein under denaturing conditions of 8M urea and the second involved separation of SDS-PAGE.


 * Image of protein:**

Amino acid sequence: >fig|1294266.3.peg.2731|VBICoxBur279880_2731| S-adenosylmethionine synthetase (EC 2.5.1.6) [Coxiella burnetii Cb175_Guyana | 1294266.3] atgacgcacacgaccttatttacctcagaatccgtttccgaaggtcacccggacaaggtg gctgaccagatttcggacgccgttcttgacgccctgattggccaagaccccaactgtcga gtggcttgcgaagccgtcgtaaaatccggaatggtttttgtggccggggaaatcaccacc aacgcctgggcggacgtagaacaaattacccgcaacaccgttttacaaatcgggtataat gacccccaccttggctttgacggggaaacgtgtgccgttgtcactgccattggaaagcaa tcgcccgatatcgtcatgggtgtcgatggccgagaagaccaagaattaggggcgggcgac caagggttaatgtttgggtacgcttctcgggaaaccgatgtttttatgccggctcccatc acgtatgcccaccgacttgtacgccaacaagccctgttgcgcaaaaatggtcgacttcct tggctgcgtcccgatgcgaaatcccaagtaaccttgcgttacgaaaacggaaaacccgtc gccgccgactgtatcgtcctttccacccaacacagccccgaaattagccaagaaagctta cgagaagccgtcatcgacgaaattatcaaacctatcatgccgccccattggctcgataaa cacacgcgtttccttgtcaatccgacagggcgcttcgttatcggcggcccagtgggggat tgcgggttaaccggtcgaaaaattatcgtcgatacctacggcgggatggcgcgccacggg ggcgggtgtttctccggcaaagatccctccaaagtggatcgttcaggcgcttatgctgcc cgttatgtagccaaaaatatcgtggctggcggcctcgccgatcgctgtgaaatacaagtt tcctatgcaattggcgttgccgagcccacttcaataagtgtcgaaactttcggcacgggg aaaattgacgaagtccgcattcagcaattaattaaagaacattttgatcttcgtcctggc aaaattatcgaagaattgaacttattgagatccatttataaggccaccgccacctacgga catttcggtcgagaagaacccgaatttacgtgggagcgaaccgataaagccgaaatactc cgtgaagccgcgggtttagccgctgcaaacgtaaccaattaa
 * Length of your protein in Amino Acids: ** 1182


 * Molecular weight: ** 98009


 * Molar Extinction: ** 20,000


 * TMpred graph: **

at ggaatctttt ttgttcacat 241 ctgaatccgt caacgaggga catcccgaca agctttgtga tcagatctcc gacgctatcc 301 tcgatgcttg ccttgaacaa gaccctgaga gcaaagttgc ttgtgagact tgtaccaaga 361 ctaacatggt catggttttt ggagaaatca ccaccaaggc taacgttgat tacgagcaga 421 ttgttcgtaa aacatgccgt gagattggat tcgtctctgc tgacgttggt ctagatgctg 481 acaattgcaa ggttctggtt aacattgagc aacagagtcc tgacattgca caaggtgttc 541 atggtcatct caccaagaag ccagaggagg ttggagctgg tgaccaaggt cacatgtttg 601 ggtatgctac tgatgagact cctgagctca tgcctcttac tcacgttctc gctactaagc 661 ttggagctaa actcactgaa gttcgcaaga atggaacttg cccttggttg aggccagatg 721 gtaagactca agtcactatt gagtacatca acgaaagcgg agccatggtt cctgtacgtg 781 tccacactgt tctcatctca acacagcatg acgagactgt gactaacgat gagatcgcag 841 ctgatcttaa ggagcatgtg atcaagccag tgatcccaga gaaatacctt gatgagaaaa 901 ccatcttcca tctcaaccca tctggtcgtt ttgttatcgg aggtcctcat ggagatgcag 961 ggcttaccgg ccgtaagatc atcatcgata cttatggtgg ttggggtgca cacggaggtg 1021 gtgctttctc tggaaaggac ccaaccaagg ttgacaggag tggggcttac atcgttaggc 1081 aagcagctaa gagcattgta gccagtgggc tagcgaggcg ggtcattgtg caagtctcgt 1141 atgccattgg tgtccctgag ccattgtctg tgttcgtgga cagttatgga acaggaaaga 1201 taccagacaa ggagattctt gagattgtga aggagagttt tgatttcagg ccaggtatga 1261 tctccattaa cttggatctg aagagaggag gtaatggtag gttcttgaag actgctgcct 1321 atggtcactt tggaagggac gatgctgatt tcacctggga ggtagtcaag ccactcaagt 1381 ctaacaaggt ccaagcttga
 * CDS Gene sequence: **


 * GC% content for gene codon: ** 47%

Do Not Need this info for Spring (but still copy these lines to your Target page for now)


 * *GC% Content for gene: **


 * output**


 * *GC% Content for gene (codon optimized): **


 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **


 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)

-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.


 * Primer design results for 'tail' primers (this is just 2 sequences): **

**