Traget-+tyrosine+hydroxylase+(T.+gonddi+Toxoplasmosis)...........Saul+H.

Format for Individual Target pages (copy this list to new Target page and then fill in for your target):
It is a parasitic disease caused by the protozoan Toxoplasma gondii. The parasite infects most genera of warm-blooded animals, including humans, but the primary host is the felid (cat) family. Animals are infected by eating infected meat, by ingestion of feces of a cat that has itself recently been infected, and by transmission from mother to fetus. Cats are the primary source of infection to human hosts, although contact with raw meat, especially lamb, is a more significant source of human infections in some countries. Fecal contamination of hands is a significant risk factor. http://tdrtargets.org/targets/view?gene_id=237217 http://www.ncbi.nlm.nih.gov/gene/21823 Gene Ontology Function: (mouse over links to read term descriptions) ** [|GO:0005506] iron ion binding** ** [|GO:0004497] monooxygenase activity**
 * *Target (protein/gene name): ** tyrosine hydroxylase
 * *NCBI Gene # or RefSeq#: 21823 **
 * *Protein ID (NP or XP #) or Wolbachia#: **033403.1
 * *Organism (including strain): Toxoplasma gondii **
 * Etiologic Risk Group (see link below): Risk group 2 **
 * *Background/Disease Information (sort of like the Intro to your Mini Research Write up): **
 * Link to TDR Targets page (if present): **
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) **
 * Essentiality of this protein: **

Process: (mouse over links to read term descriptions) ** [|GO:0055114] oxidation reduction** ** [|GO:0009072] aromatic amino acid family metabolic process** http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.14.16.2
 * Complex of proteins?: no **
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **
 * no chemical ****compounds**
 * *EC#:1.14.16.2 **
 * Link to BRENDA EC# page: **

Continuous Spectrophotometric Rate Determination, 100 mM Tris HCl Buffer, pH 7.0 at 37 ° C (Buffer), 30 mM L -Tyrosine Solution, pH 7.0 at 37 ° C ( L -Tyr) http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/learning-center/assay-library/ec-number.html http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/General_Information/tyrosine_hydroxylase.pdf
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **
 * -- link to Sigma (or other company) page for assay (see Sigma links below) **
 * -- links to assay reagents (substrates) pages. **
 * --- List cost and quantity of substrate reagents, supplier, and catalog # **

6,7-Dimethyl-5,6,7,8-Tetrahydropterine 100 mg in glass bottle for $105.00. 1l Tris HCL $27.00, L-Tyrosine Solution 50g for $65.50 PDB # 2MDA
 * Structure Available (PDB or Homology model) **
 * Expression Information (has it been expressed in bacterial cells): ** yes in escherichia coli
 * Purification Method: ** one of many purification methods is chromatography
 * Image of protein (PyMol with features delineated and shown separately): **

code PTPDATTPQAKGFRRAVSELDAKQAEAIMVRGQGAPGPSLTGSPWPGTAAPAASYTPTPR SPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAVLNLLFSPRA TKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQ VSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIA FQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLERFSGYREDNIP QLEDVSRFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCCH ELLGHVPMLADRTFAQFSQDIGLASLGASDEEIEKLSTLSWFTVEFGLCKQNGEVKAYGA GLLSSYGELLHCLSEEPEIRAFDPEAAAVQPYQDQTYQSVYFVSESFSDAKDKLRSYASR IQRPFSVKFDPYTLAIDVLDSPQAVRRSLEGVQDELDTLAHALSAIG code
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **
 * *length of your protein in Amino Acids: 527 **
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website: 58392.9 **
 * Molar Extinction coefficient of your protein at 280 nm wavelength: **46215 M^-1 cm^-1
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.

code >1575 bp ATGCCCACCCCCGACGCCACCACGCCACAGGCCAAGGGCTTCCGCAGGGCCGTGTCTGAG CTGGACGCCAAGCAGGCAGAGGCCATCATGGGCGCCCCGGGGCCCAGCCTCACAGGCTCT CCGTGGCCTGGAACTGCAGCCCCAGCTGCATCCTACACCCCCACCCCAAGGTCCCCGCGG TTCATTGGGCGCAGGCAGAGCCTCATCGAGGACGCCCGCAAGGAGCGGGAGGCGGCGGTG GCAGCAGCGGCCGCTGCAGTCCCCTCGGAGCCCGGGGACCCCCTGGAGGCTGTGGCCTTT GAGGAGAAGGAGGGGAAGGCCGTGCTAAACCTGCTCTTCTCCCCGAGGGCCACCAAGCCC TCGGCGCTGTCCCGAGCTGTGAAGGTGTTTGAGACGTTTGAAGCCAAAATCCACCATCTA GAGACCCGGCCCGCCCAGAGGCCGCGAGCTGGGGGCCCCCACCTGGAGTACTTCGTGCGC CTCGAGGTGCGCCGAGGGGACCTGGCCGCCCTGCTCAGTGGTGTGCGCCAGGTGTCAGAG GACGTGCGCAGCCCCGCGGGGCCCAAGGTCCCCTGGTTCCCAAGAAAAGTGTCAGAGCTG GACAAGTGTCATCACCTGGTCACCAAGTTCGACCCTGACCTGGACTTGGACCACCCGGGC TTCTCGGACCAGGTGTACCGCCAGCGCAGGAAGCTGATTGCTGAGATCGCCTTCCAGTAC AGGCACGGCGACCCGATTCCCCGTGTGGAGTACACCGCCGAGGAGATTGCCACCTGGAAG GAGGTCTACACCACGCTGAAGGGCCTCTACGCCACGCACGCCTGCGGGGAGCACCTGGAG GCCTTTGCTTTGCTGGAGCGCTTCAGCGGCTACCGGGAAGACAATATCCCCCAGCTGGAG GACGTCTCCCGCTTCCTGAAGGAGCGCACGGGCTTCCAGCTGCGGCCTGTGGCCGGCCTG CTGTCCGCCCGGGACTTCCTGGCCAGCCTGGCCTTCCGCGTGTTCCAGTGCACCCAGTAT ATCCGCCACGCGTCCTCGCCCATGCACTCCCCTGAGCCGGACTGCTGCCACGAGCTGCTG GGGCACGTGCCCATGCTGGCCGACCGCACCTTCGCGCAGTTCTCGCAGGACATTGGCCTG GCGTCCCTGGGGGCCTCGGATGAGGAAATTGAGAAGCTGTCCACGCTGTCATGGTTCACG GTGGAGTTCGGGCTGTGTAAGCAGAACGGGGAGGTGAAGGCCTATGGTGCCGGGCTGCTG TCCTCCTACGGGGAGCTCCTGCACTGCCTGTCTGAGGAGCCTGAGATTCGGGCCTTCGAC CCTGAGGCTGCGGCCGTGCAGCCCTACCAAGACCAGACGTACCAGTCAGTCTACTTCGTG TCTGAGAGCTTCAGTGACGCCAAGGACAAGCTCAGGAGCTATGCCTCACGCATCCAGCGC CCCTTCTCCGTGAAGTTCGACCCGTACACGCTGGCCATCGACGTGCTGGACAGCCCCCAG GCCGTGCGGCGCTCCCTGGAGGGTGTCCAGGATGAGCTGGACACCCTTGCCCATGCGCTG AGTGCCATTGGCTAG code code ATGCCCACCCCCGACGCCACCACGCCACAGGCCAAGGGCTTCCGCAGGGCCGTGTCTGAG CTGGACGCCAAGCAGGCAGAGGCCATCATGGGCGCCCCGGGGCCCAGCCTCACAGGCTCT CCGTGGCCTGGAACTGCAGCCCCAGCTGCATCCTACACCCCCACCCCAAGGTCCCCGCGG TTCATTGGGCGCAGGCAGAGCCTCATCGAGGACGCCCGCAAGGAGCGGGAGGCGGCGGTG GCAGCAGCGGCCGCTGCAGTCCCCTCGGAGCCCGGGGACCCCCTGGAGGCTGTGGCCTTT GAGGAGAAGGAGGGGAAGGCCGTGCTAAACCTGCTCTTCTCCCCGAGGGCCACCAAGCCC TCGGCGCTGTCCCGAGCTGTGAAGGTGTTTGAGACGTTTGAAGCCAAAATCCACCATCTA GAGACCCGGCCCGCCCAGAGGCCGCGAGCTGGGGGCCCCCACCTGGAGTACTTCGTGCGC CTCGAGGTGCGCCGAGGGGACCTGGCCGCCCTGCTCAGTGGTGTGCGCCAGGTGTCAGAG GACGTGCGCAGCCCCGCGGGGCCCAAGGTCCCCTGGTTCCCAAGAAAAGTGTCAGAGCTG GACAAGTGTCATCACCTGGTCACCAAGTTCGACCCTGACCTGGACTTGGACCACCCGGGC TTCTCGGACCAGGTGTACCGCCAGCGCAGGAAGCTGATTGCTGAGATCGCCTTCCAGTAC AGGCACGGCGACCCGATTCCCCGTGTGGAGTACACCGCCGAGGAGATTGCCACCTGGAAG GAGGTCTACACCACGCTGAAGGGCCTCTACGCCACGCACGCCTGCGGGGAGCACCTGGAG GCCTTTGCTTTGCTGGAGCGCTTCAGCGGCTACCGGGAAGACAATATCCCCCAGCTGGAG GACGTCTCCCGCTTCCTGAAGGAGCGCACGG code
 * *CDS Gene Sequence (paste as text only): **
 * *GC% Content for gene: 66.05% **
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **
 * *GC% Content for gene (codon optimized): 50.3% **

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)

**
 * Primer design results for 'tail' primers (this is just 2 sequences): **

Resources:
See ** ProtocolTargetDiscoveryVDS.docx ** for more

Etiologic Risk Group Categories (for pathogens): http://www.utexas.edu/research/rsc/ibc/agent_class.html#_Toc7238334


 * SIGMA-ALDRICH RESOURCES **

Enzyme Explorer

@http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer.html

Enzyme Classification Index (EC number)

@http://www.sigmaaldrich.com/life-science/biochemicals/biochemical-products.html?TablePage=14573088

WolframAlpha @http://www.wolframalpha.com/

DrugBank @http://www.drugbank.ca/


 * Databases of genes/organisms: **

@http://www.niaid.nih.gov/Pages/default.aspx

@http://eupathdb.org/eupathdb/

@https://patricbrc.vbi.vt.edu/portal/portal/patric/Home

@http://www.nmpdr.org/FIG/wiki/view.cgi/Main/EssentialGenes

@http://tubic.tju.edu.cn/deg/

@http://csgid.org/csgid/cake/pages/community_request_gateway

@http://tdrtargets.org/

@http://gsc.jcvi.org/status.shtml


 * Scientific Nomenclature page from Center for Disease Control (gene, protein names and abbreviations) **

@http://wwwnc.cdc.gov/eid/pages/scientific-nomenclature.htm


 * Gene Information: **

NCBI GENE Page: @http://www.ncbi.nlm.nih.gov/gene

BLAST Page: @http://blast.ncbi.nlm.nih.gov/


 * Protein Information: **

NCBI Protein Page: @http://www.ncbi.nlm.nih.gov/protein

Protein Expression Website

Protein Expression Paper:


 * Primer Overlap PCR Articles **






 * Is my target good for Virtual Screening programs? **