SANIYA+H.

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__ Introduction: __

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__ Results: __

Figure 1a: LB Agar Amp plate of BL21(DE3) E. coli bacteria with DNA plasmid pGEM-gbr22 and SOC media after 14 hours in a 37°C incubator. Small purple dots are indicative of colonies of bacterial growth.

Figure 1b: Control LB Agar Amp plate of BL21(DE3) E. coli bacteria with SOC media, without DNA plasmid after 15 hours in a 37°C incubator.

Figure 1c: LB agar plate without antibiotic of a swab of the dirt on the floor near lab refrigerator after about 15 hours in a 37°C incubator; "Fun Plate."

Figure 2: Two 125mL Erlenmeyer flasks of LB and 100ug/ml ampicillin with BL21(DE3) E. coli bacteria transformed with DNA plasmid pGEM-gbr22 after 24 hours in a 37°C shaking incubator. Purple/pink color originated from purple protein produced by bacteria.

Figure 3: Purple cell pellets of BL21(DE3) E. coli bacteria transformed with DNA plasmid pGEM-gbr22 after 10 minutes in a 4°C centrifuge at 5,000 rpm. Excess liquid decanted. Pellet weight recorded as 0.54 g.

Figure 4: PAGE gel of 6 samples of pgbr22. Columns: molecular weight standard, cell lysate, soluble fraction with pellet, flow through, wash (20mM imidazole), elution 1, and elution 2 (both elutions done with 1xPBS and 250mM imidazole).

Figure 5: Pre-stained molecular weight standard ladder used in lane 1 of gel sample.

__ Discussion: __

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