Target+-+UDP-N-acetylglucosamine+pyrophosphorylase+(Aspergillus+fumigatus)

__TARGET NAME:__ UDP-N-acetylglucosamine pyrophosphorylase __GENE NAME:__ AFUA_7G02180
 * Target: **
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70982372 66844338
 * NCBI Gene # or RefSeq#: **
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4BMA.10.2210
 * Protein ID (NP or XP #) or Wolbachia#: **

__Scientific Name:__ //Neosartorya fumigata// __Also Known As:__ //Aspergillus fumigatus// __Strains:__ ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)
 * <span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;">Organism: **
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<span style="font-family: Arial,Helvetica,sans-serif;">Risk Group 2
 * <span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;">Etiologic Risk Group (see link below): **
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====<span style="font-family: Arial,Helvetica,sans-serif;">Neosartorya fumigata is most commonly known as Aspergillus fumigatus and is the predominant cause allergic and invasive aspergillosis. The fungus is a primary pathogen of the sinuses, lungs, damaged skin, and subcutaneous tissue. It has the ability to spread to other organs including the brain. The medications used against ==== ====<span style="font-family: Arial,Helvetica,sans-serif;">Aspergillus fumigatus are azoles like voriconazole, however the fungus is beginning to build resistance to the drugs. In fungus the synthasis of chitin is an essential component of the cell wall. The sugar nucleotide UDP-N-acetylglucosamine (UDP-GLCNAc) is essential in the production of this chitin. The enzymeUDP-N-acetylglucosamine pyrophosphorylase is the enzyme that is essential in the production of the sugar nucleotide (UDP-GLCNAc). In conclusion, this enzyme is a key target when it comes to the Aspergillus fumigatus disease. The general idea is to inhibit the enzyme, which will stop the production of the essential sugar nucleotide, then no chitin will be made, and the cell wall will corrupt killing the fungus. ====
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**<span style="background-color: #ffffff; font-family: Arial,sans-serif; font-size: 12pt;">Figure 1: Microscopic image of //Aspergillus fumigatus// **

**<span style="background-color: #ffffff; font-family: Arial,sans-serif; font-size: 12pt;">Figure 2: How //Aspergillus fumigatus// invades the lungs ** <span style="font-family: Arial,Helvetica,sans-serif;"> There were no links for the organism Aspergillus fumigatus. The link given below is for other organisms.
 * <span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;">Link to TDR Targets page (if present): **
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<span style="font-family: Arial,Helvetica,sans-serif;"> There were no links for the organism Aspergillus fumigatus on Gene Database <span style="font-family: Arial,Helvetica,sans-serif; line-height: 1.5;">I was having trouble finding a link for the organism I chose. The links provided show the enzyme with other organisms. <span style="font-family: Arial,Helvetica,sans-serif;">The closest I got was this:
 * <span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;">Link to Gene Database page: **
 * <span style="font-family: Arial,Helvetica,sans-serif;">[|http://www.genedb.org/Query/quickSearch?product=true&allNames=true&d-16544-p=1&searchText=UDP-N-acetylglucosamine+pyrophosphorylase&taxons=Root&pseudogenes=true&y=0&x]
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 * http://www.rcsb.org/pdb/explore/explore.do?structureId=4BMA
 * http://www.ebi.ac.uk/pdbe-srv/view/entry/4bma/summary.html

**<span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;">Essentiality of this protein: ** <span style="font-family: Arial,Helvetica,sans-serif;">Inferred from electronic annotation. Source: UniProtKB-EC
 * [|UDP-N-actylglucosamine diphosphorylase activity]

**<span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;">Complex of proteins?: ** <span style="font-family: Arial,Helvetica,sans-serif; line-height: 1.5;">None were given.

**<span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;">Druggable Target: ** <span style="font-family: Arial,Helvetica,sans-serif;"> None were given.

**<span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;"> EC#: ** <span style="font-family: Arial,Helvetica,sans-serif;">[|2.7.7.23]
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**<span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;">Link to BRENDA EC# page: **
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**<span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;">Screenshot of BRENDA enzyme mechanism schematic ** <span style="font-family: Arial,Helvetica,sans-serif;">There was not one present.

**<span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;">Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): ** <span style="font-family: Arial,Helvetica,sans-serif;">Link to Sigma page for assay (see Sigma links below):
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<span style="font-family: Arial,Helvetica,sans-serif;">__Product Number__: U4375 __CAS Number__: 91183-98-1 __EC Number:__ 293-820-5 __MDL Number:__ MFCD00799985 __Supplier:__ Sigma-Aldrich **<span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;"> Structure Available (PDB or Homology model) ** PDB #: 4BMA
 * Pack Size || Price (USD) ||
 * 25 MG || 54.60 ||
 * 100 MG || 185.50 ||
 * 500 MG || 564.00 ||
 * 1G || 972.00 ||
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**<span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;">Current Inhibitors ** <span style="font-family: Arial,Helvetica,sans-serif;"> GOL- Glycerol More info on this website: **<span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;">Expression Information: ** <span style="font-family: Arial,Helvetica,sans-serif;">Has been expresses in //Escherichiu coli//
 * http://www.bindingdb.org/bind/luceneResult.jsp?thisInput=UDP-N-acetylglucosamine+pyrophosphorylase&submit=Go

**<span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;">Purification Method: ** http://www.ncbi.nlm.nih.gov/pubmed/17142897
 * <span style="font-family: Arial,Helvetica,sans-serif; font-size: 17px;">Image of protein: **

** Figure 3: PDB image of the UDP-N-acetylglucosamine diphosphorylase enzyme ** **<span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;">Amino Acid Sequence: ** <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;"> MAVAIKETVSNFMERFHGHPENLPREPSAEEFQQLRKKYTDAGQGHVFAFV  <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">DELQTGERSQLFHQLSSFDPVRINELADKALNPPKADDGPASLEPLPDI  <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">TASILDSDPKDLEQWYEEGLKLVAGNKVAVVLMAGGQGTRLGSSAPKGCF  <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">DIGLPSHKSLFQIQAERIAKLQLLAQRISG.KEAVIPWYVMTSGPTRKPT  <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">EEFFEQHKYFGLNKSDVIIFEQGVLPCISN.EGKILMESKFKVAVAPDGNG  <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">GIYQALLTSGVREDMRKRGIEHIHTYCVD.NCLVKVADPVFIGFAASKQV  <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">DIATKVVRKRNATESVGLILQKNGKPDVVE.YSEIDKETAEAKDPKQPDVL  <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">KFRAANIVNHYYSFKFFESIELWAHKLPHH.VARKKIPCIKEGTGEFFKPE  <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">KPNGIKLEQFVFDVFPMTPLEKFACIEVRR.EDEFSPLKNARGTGEDDPDT  <span style="font-family: Arial,Helvetica,sans-serif; text-align: left;">SKRDIMSQGQRWIEKAGGIVITEGDVVGVEVSPLISYGGEGLEFLKGREIKAPAFIEKEE
 * <span style="font-family: Arial,Helvetica,sans-serif; line-height: 1.5;">[|http://www.uniprot.org/uniprot/Q4WAR0#section_terms]

** Length of your Protein in Amino Acids: **  509


 * Molecular Weight: **  56715.7kDal
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**<span style="font-family: Arial,Helvetica,sans-serif; font-size: 130%;">Molar Extinction coefficient of your protein at 280 nm wavelength ** <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;"> __Assuming all pairs of Cys residues form cystines:__ 37275  <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">__Assuming all Cys residues are reduces:__ 3690 <span style="font-family: Arial,Helvetica,sans-serif; text-align: left;">** TMpred Graph Image <span style="background-color: #ffffff; font-family: Arial,sans-serif;">: ** **CDS Gene Sequence (paste as text only):** Not given. Primer did not generate the information. Primer did not generate the information.
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 * GC% Content for gene: **
 * CDS Gene Sequence (codon optimized) - copy from output of Primer **
 * Design Protocol (paste as text only): **

Primer did not generate the information.
 * GC% Content for gene (codon optimized): **

Resources:
See ** ProtocolTargetDiscoveryVDS.docx ** for more Etiologic Risk Group Categories (for pathogens): http://www.utexas.edu/research/rsc/ibc/agent_class.html#_Toc7238334

Enzyme Explorer @http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer.html
 * SIGMA-ALDRICH RESOURCES **

Enzyme Classification Index (EC number) @http://www.sigmaaldrich.com/life-science/biochemicals/biochemical-products.html?TablePage=14573088

WolframAlpha @http://www.wolframalpha.com/ DrugBank @http://www.drugbank.ca/

@http://www.niaid.nih.gov/Pages/default.aspx @http://eupathdb.org/eupathdb/ @https://patricbrc.vbi.vt.edu/portal/portal/patric/Home @http://www.nmpdr.org/FIG/wiki/view.cgi/Main/EssentialGenes @http://tubic.tju.edu.cn/deg/ @http://csgid.org/csgid/cake/pages/community_request_gateway @http://tdrtargets.org/ @http://gsc.jcvi.org/status.shtml
 * Databases of genes/organisms: **

@http://wwwnc.cdc.gov/eid/pages/scientific-nomenclature.htm
 * Scientific Nomenclature page from Center for Disease Control (gene, protein names and abbreviations) **

NCBI GENE Page: @http://www.ncbi.nlm.nih.gov/gene BLAST Page: @http://blast.ncbi.nlm.nih.gov/
 * Gene Information: **

NCBI Protein Page: @http://www.ncbi.nlm.nih.gov/protein Protein Expression Website Protein Expression Paper:
 * Protein Information: **


 * Primer Overlap PCR Articles **


 * Is my target good for Virtual Screening programs? **