Target-serine-threonine+protein+phosphatase.+(P.+falciparum+3D7)

Malaria is a life-threatening disease caused by protozoan parasites called Plasmodia. This disease is transmitted to humans from the bites of infected Anopheles mosquitoes. Currently, there are four types of malaria species that have been found to affect humans. They consist of Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale.
 * Target (protein/gene name): Serine-threonine protein phosphatase**
 * *NCBI Gene # or RefSeq#: 2655328 **
 * Protein ID (NP or XP #) or Wolbachia#: MAL7P1.230**
 * Organism (including strain): Plasmodium falciparum 3D7 (isolate:3D7)**
 * Etiologic Risk Group (see link below): Risk group 3**
 * Background/Disease Information (sort of like the Intro to your Mini Research Write up):**
 * Link to TDR Targets page (if present):** http://www.tdrtargets.org/targets/view?gene_id=533
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.)** http://www.ncbi.nlm.nih.gov/gene/2655328
 * Essentiality of this protein:**
 * Complex of proteins?:n/a**
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): .5**


 * *EC#: 3.1.3.16 **
 * Link to BRENDA EC# page:** http://www.brenda-enzymes.org/php/result_flat.php4?ecno=3.1.3.16
 * --** Show screenshot of BRENDA enzyme mechanism schematic

http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/learning-center/assay-library/ec-number-iii.html http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Enzyme_Assay/calcineurin.pdf http://www.sigmaaldrich.com/catalog/search?interface=All&term=serine-threonine%20protein%20phosphatase&N=0&focus=product&lang=en®ion=US $297.50 for .1ML Supplier: Sigma Aldrich (from Saint Louis) CAS#: 0000000000
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**
 * -- link to Sigma (or other company) page for assay (see Sigma links below)**
 * -- -or link (or citation) to paper that contains assay information**
 * -- links to assay reagents (substrates) pages.**
 * --- List cost and quantity of substrate reagents, supplier, and catalog #**

-- PDB # or closest PDB entry if using homology model: GAP50 -- For Homology Model option: Show pairwise alignment of your BLASTP search in NCBI against the PDB
 * Structure Available (PDB or Homology model)**

Query Coverage:99% Max % Identities:100% % Positives 100% Chain used for homology: Chain A

TATTTTTGTCAAAAATTTTTATATTTCTTTGATGTTCCGTTCTATAATTTCACGAAAATGCATTTGTCAG TAGTCTTGAATTCAATATATACGTCTTTTTTTATTAAACAAAAAAAAAAAAAAACTTCTTATTAATAATG TAAAAAAATAAAAGGAAAGAATAATAAGTGTTTCTTTCTTTTTATTTATTTATTTATCTATTTATTTTTC TTTCTTTTCCTTTTTCTTTTTTGAATAATTAAAAAAATAAACCATTTAAAAAGAAAGTAAAAATAATTAT CTATACATAAATAATTATACATATATATATATACAAAATTATGGAACCACTGCCTGATCCGAAGAATGAT AGACAAGTGAAGGATGTTGAGCCTCCACCAGCAAAAGTAAGAAGAATACTGATTAAGAATTTTATCTATC ATTATGAATATTTATGTCTAATTTGCTTTTTTATTTTATATTTATAATTTTAACATTTTCATAAATATAT AATATACATTCGTAATTTGGAACACACCCATATAATATATATATATATATATTTATTTATTTATTTATTT ATATTTCCTATACTAGCTAGCCAAAAGATAAATTATGAACAATTCATAATTTTGTTAATTTTGTTCATAT TATTTTCCTTATTTTTTTATTTTTTATTTTTTTATTTTTTATTTTTTATTTTTTTATTTTTTTATTTAGC CGTTAAGCTTAGAACTACTATATCCAAACGGAACGGAAGAGCCACCAGATTATAAAGCTCTAAGAGATCA TTTAAAAAAAGAGGGTCGCATAAGAAAAGAAGATTGTTTAGATATAATAAAGAAGGTGATAGATATAGTA AGCAATGAACCGAATTTATTGAGGTTAAAGGACCCAATAACAATTGTTGGAGATATTCATGGTCAGTATT ATGATTTATTAAAATTATTGGAAGTTGGAGGTAATCCTGATCATACTCAATTTTTATTTTTAGGAGATTA TGTCGATAGGGGTTCGTTTAGTATAGAAGTATTATTATTATTATATGCTTTAAAAATAAATTTCCCTGAT CGCATATGGTTAATACGAGGAAATCATGAATGTCGTCAAATGACAACATTTTTTAATTTTAGAGATGAAT GTGAATATAAGTATGATATAGTAGTATATTATGCTTTTATGGAATCTTTTGATACAATTCCTTTATCAGC TGTAATAAATGGTAAATTTTTAGGAGTTCATGGTGGTTTATCTCCTGATTTAATATTATTGAATCAAATA TGTTCATTTACTCGATTTCAAGAACCTCCACGTTCAGGAATTTTCTGTGACATATTATGGTCAGATCCTA TTGATGAAGATAAAGAAGAGCATACCATACAAACTGAATCTTATTTTCCTAATGACATAAGAGGATGTAG TTATTTTTTTGGATACAATGCAGCAACAACATTTTTAGAAAAAAATGGTTTATTATCAATAATACGAGCC CACGAAGCTCAGCTTGAAGGATATAAAATGCATCAGACAAATTTAAAGACTGGTTTTCCCATAGTTATAA CTATATTTTCAGCTCCTAATTATTGTGATGTTTATAATAACAAAGGTGCCGTTTTAAAATTCGATAGTAA CACATTAAATATACAACAATTTAGTTTTTCACCTCATCCTTACCATTTACCTAATTTTATGAATTTATTT ACATGGTCCTTACCATTTGTAAGCGAAAAAGTTACGGAAATGTTGTATTCCCTTTTAAATTGTAGTATGA ATGATACGGATGAAGGGATAAACGATATCGTTTTACCTAAAGAAGTAATACAAATTTTAAATTATATTGA AGAAAATAATAAAAGAATTAATGAAATGAATTTTAATAACAACGATGATAATGTACAATATGAAGATAAT GGTCCATATATAAATCAAAGTAATAATAATAATAATAATAATAATAAGGATAACAAGTTTGATGATATTA CTTATGATGATCATAAAAAAGAGAAAGACAAAAGAAATAAAATATCTTCTAATGGTAATATGCAAGATAA TAATCAATTATATGATCATTCAGAAGGACATAATAATTATAATGATGAAGATGAATTCTTCAAAAATGTT AAAAAGACAGATACAAATAATAATAATAATAATGAGGAAGAGGACGAGGAAGAGGACGAGGAGGAGGAGG AGGATGGAAGAAAAACTAAAGATGTAGGACAAGTATCTAAAGAAAGAACGGATACTCTAAGAAAAAAAGT ACAATCGGTAGGTAGACTAATGAGAGTTTTTAGAACCTTAAGGAAAGAAAATGAATTAATTGTGCAACTT AAAGGATGTAGTCCTGGTTATAGAATACCTGTAGGGTTATTATTAAGTGGAAAAGAGGGATTGGAAAATG AGCTCGAAAAATTTACAAAGGTTAAAGAAATTGATAGTATTAACGAAAAGAGGCCATCCAACGAATAAAA AAAAAAAAATAATAAATAAATAAAAATAAATGAATGAATGAATAAATGAATAAACAAATAAATGAATGTA TGAATAAATGAATAAACAAATAAATGAATGTATGAATAAATGAATAAACAAATAAATGAATGTATGAATA AATGAATAAACAAATAAATGAATGTATGAATAAATGTGTTTGTGTGGATGGATTGGTTTATGAGTATTTA CAAATAAAATGTAAAAATTATGATAAAATAAGAAAATCATAGTACATAGGAATAGAAGAACATACAACAC AATTTGTATACATACATACATATGTATATATATATA Ext. coefficient 17125 Abs 0.1% (=1 g/l) 0.075, assuming all pairs of Cys residues form cystine Ext. coefficient 0 Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
 * Current Inhibitors:info could not be found**
 * Expression Information (has it been expressed in bacterial cells):**
 * Purification Method:n/a**
 * Image of protein (PyMol with features delineated and shown separately):**
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):**
 * length of your protein in Amino Acids 2766**
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website :228446.7**
 * Molar Extinction coefficient of your protein at 280 nm wavelength:**
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
 * CDS Gene Sequence (paste as text only): info could not be found**
 * GC% Content for gene:**
 * CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):**
 * GC% Content for gene (codon optimized):**

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**
 * ( link to DNA Works output text file - **that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences):**