MentorsSp14

@Calendar for Staff Sp14
Use the MentorLabLog on GDocs/VDSstaff for as a Lab Notebook for Wet Lab tasks

@Teaching Points for Labs Sp14
-- also see folder in VDS Staff Google Docs account -- contains lab handout and teaching points sheets

MENTORS: **ANITA**, **TONY**, **GRACE**, **KAVYA**, **NICOLE**, **PRIYA**, **SERENA**, **VICKY**, **MELISSA**, TA**: JOEY**

NOTE: the best way to figure out what you need to work on is to do 'Ctrl+F' and search for your name. Hit 'enter' to find the next instance. - Thanks, Dr. B - this assumes I haven't misspelled your name!


 * Packages: ** Open, figure out if it is VDS, show to Owais if there, Label appropriately, Place in proper storage, Give receipt to Dr. B and let him know it has arrived and is stored

Please fill out your weekly schedule here: https://docs.google.com/spreadsheet/ccc?key=0AvGl3lMyhDsodDBwM3ZZQ3RpbVVMbVlpdDd1bFFXVkE&usp=sharing

- show them Alt + i + t for time stamp - have them open and use UTBox - how to force Firefox to download a file to a folder you specify - don't need pics for Warmups - their Word doc does not need to be typed verbatim from handout. They can copy and paste the procedure steps, but must add in comentary/analysis
 * NOTES for PyMol 1**

TA TASKS- Joey
Lab notebook grading

=MENTOR TASKS=


 * Week of MLK**

-- determine # of days late for each student using the Image file in the same folder that has the submission dates __DUE DATE__: Monday during the day - so that Dr. B can hand them back in class on Tuesday
 * Lit Search and Bibliography assignment reviewing** (see **ReviewingLitSearchforMentorsGDocsSp14** in VDSStaff GDocs) - **KAVYA, NICOLE**
 * --** copies are in Dr. B's Pigeon Hole, write directly on the papers and indicate points off out of total points and then indicate # of points off for days late.

-- After the students turn their lab notebooks in on Friday and Saturday, you can begin commenting. -(they turn them in on Saturday at the latest - but once a grading rubric is on there - you can start looking at it.) -- for the 4-5 notebooks which you have been assigned, go through them and write comments directly in the notebook (blu ink preferred) -- Also, as you go, please circle or underline items on the Lab Notebook Grading Rubric that would help Joey in grading. (e.g. missing analysis, materials not listed, no page numbers, etc.) -- Joey will go back through and apply a score to each one. Then Dr. B will approve them. __DUE DATE__: Monday night/Tuesday during the day of PyMol1 week - so that Joey and I have time to look at them.
 * Comment on Lab Notebooks** (see your assignments on the **LabNotebookCommentingforMentorsSp14** in VDSStaff GDocs) - **ALL**

'Starter Questions' for the TEAM meetings - to help your students get to know you better
QUESTIONS FOR THE WHOLE GROUP
 * Favorite place on the Drag (or around the 40 acres but not on campus)?


 * Where are you from? What high school did you go to?
 * How is your adjustment to UT going?
 * Favorite/Least favorite __#|classes this__ semester? Any particular classes you are concerned about?


 * What are your majors?
 * What are your career interests?


 * What do you think of VDS so far? Favorite aspects? Least favorite aspects?
 * Which do you prefer - wet lab or computer labs?
 * Do you have any concerns about the labs?
 * Do you have any questions regarding the concepts underlying the labs?


 * What do you hope to get out of this experience?
 * What are your research interests?
 * Since this is VDS, what is your favorite drug/molecule?

General points to cover (by the TEAM leaders):
- basic intro (major, year, etc.) - overview of what they can expect from VDS (short- and long-term) - how VDS has helped you and why it is beneficial for them to stick with it - your research interests and experiences - your career plans and what stage you are at now - Why VDS is the best stream!

From the Mini-Research Write Up Page (wait until they have turned in their 1st Mini Research Write Up as PDF.) --**Create a Wikispaces page** for each Disease (no student name on there)

Modify LabNotebook Reviewing assignments sheet in VDSStaff GDocs. -- update roster - DONE JOEY -- for each student in the spreadsheet - put in their Team name in a new column - DONE JOEY -- assign each mentor to the students in their group -DONE Dr. B

Teaching Assistant's Stuff - Joey
Go over Lab Notebook Grading Rubric & Lab Notebook spreadsheet Make Sign-In Sheet for Lab (for PAI 2.14 folder) - will probably be 2 sheets each week (in Gdocs, download, edit and re-upload)

ADMINISTRATIVE/OFFICE WORK
come up with a lab 'mission statement' - **ALL** Please Print, Read and Sign the Mentor Contracts. Then return to Dr. B - **ALL** --- Mentor Contract on GDocs update mentor schedules - **DONE** update Class Schedule - **GRACE** Take pictures of staff (group and individuals - upload to VDSStaff in GDocs, Upload to Wiki Mentors Sp14 page) - **DONE** Generate a time sheet - **PAID MENTORS**

post Labnotebook Grading rubric to Wiki (upload PDF then in space.menu on lhs - make link to file) - **ANITA** post Labnotebook vs LabReport to Wiki (upload PDF then in space.menu on lhs - make link to file) - **VICKY**

In 'Pigeon Holes' for lab notebooks
organize pigeon holes by moving VDS Alums to the other lab notebook stacks - **TONY (__DONE__)** make new name plate sheets (out of manila folders) - **KAVYA - (__DONE__)**

organize binders (Student one and Mentor one) - **DONE** Mentor Folder: Student Folder:
 * 1) print new timesheet pay periods sheet (from UT webpage - Timesheet Pay Periods and Due Dates (see bottom of link)
 * 2) create and print new hourly sign-in sheet (don't need to do TA though) **- see old one in GDocs either VDSStaff or /Mentors**
 * 3) make tabs for each mentor - see GDocs/Mentors/ Schedule sheet to get Mentor names (dont do TA because she does not do timesheets) -
 * 4) get some extra blue sheets to include in folder
 * 1) remove old safety training (except for people still working in lab (e.g. mentors and researchers)
 * 2) put new sign in sheet for students (get from the TA)
 * 3) make picture roster of students and staff pics (get photo roster from Dr. B - but add in staff pictures)

make Mentor name tags with VDS logo on them (talk to Dr. B - may have to use Dr. B's printer? Might work on lab printer in manual feed mode) - **DONE**

create a TEAMS Sp14 page in Wikispaces add link in the menu on the left hand side of Wikispaces create **TEAMS** by of about 5-7 students (#of students/# of mentors) per Team by UTEID alphabetical Make a Google Spreadsheet in GDocs/VDSclass/Misc that shows all of the Team, Mentor leaders, and team name - embed this spreadsheet into the Wikispaces page (Grace needs to learn embedding too - so get her when Dr. B shows you this step) Create the same TEAMS in Piazza - Dr. B needs to make the Piazza page first - add link to Wikispaces to Piazza (place on **space.menu** on left and on **TEAMS Sp14** page) ANNOUNCE TEAMS IN VDS 1st Class ???
 * TEAMS (on Piazza and Wikispaces) - NICOLE**

Once TEAMS are made - edit the TEAMS SIgns Sp14.docx in the VDSStaff GDocs/Misc and let Dr. B know (he will use it in class) - **NICOLE**

WIKISPACES WORK

 * 1) create Wikispaces pages for each student for Protein Labs (create new page Protein Labs Sp14) - **ANITA**

-- place receipts in Dr. B's receipts pigeon hole
 * CATALOGING PURCHASING INTO EXCEL SPREADSHEET FROM FALL 13**

Lab Safety
create Lab Safety Sp14 page in Wikispaces with names of each students and table, link from left hand menu - **PRIYA (done -Anita Thanks Anita <3 )** Practice Lab Safety Walk Throughs - **TONY, ANITA, PRIYA (__DONE__)** Lab Safety Videos posted to Wikispaces and Generate Quizzes in Canvas based on Procko work - **TONY, ANITA**

LAB WORK
clean lab - **ALL** clean out top loading -20degC freezer - remove old Biobricks stuff and create a new label for VDS on a metal rack. - **PRIYA** organize and label -80degC freezer racks - **DONE** unpacking supplies, labeling and storing them Transformation of pGBR22 in DH5alpha AMP plates - **PRIYA** Blue Juice for protein gels - **PRIYA (Waiting on DTT)** Make some YopH -- **Tony** Transformation, Expression, Sonication (__**DONE**__) -- **Nicole** Purification (Ni-NTA), FPLC, Storage in Glycerol walk through Buffer & Solutions -
 * First pipetting steps with __Excel graph__ of Buffers & Solutions **KAVYA, GRACE**
 * 200 ml 0.1 M Tris Base __pH 7.5 – pH meter, acids (calibrate pH meter as part of this, test out new pH probes) __ **- DONE**
 *  Kanamycin – __syringe filte __r - **SERENA**

THIS WEEK: walk through the Beer’s Law lab - **PRIYA, KAVYA** walk through Titration lab - **DONE** walk through virtual labs

Rinse the 'clean' collirollers in nanopure water, transfer to a clean bottle, label - then Autoclave (use autoclave tape) - **SERENA** Autoclave Nanopure water, label, store in Cubby. Make 2 x 500ml. - this is for 1st Lab to make 30% EtOH - **GRACE** Autoclave colirollers - **SERENA** divide up colirrolers in to 6 rollers each in sterile tubes of (600 ul tubes) - make a full ~ 80 tube rack of these - **SERENA & GRACE**

Pre and Post Surveys (SURE research surveys are online) - **DONE**
 * ASSESSMENT**
 * look up how to use and implement

Update Laptops and Desktops - **TONY, KAVYA (__DONE__)**
 * Clean off old icons from Desktops of __Desktop__ & __Laptop__ computers
 * Do Windows Updates on laptops

**SOLUTIONS INSTRUCTIONS:**

 * (also see the Solutions&Dilutions Spreadsheet on GDocs for help with calculations)**


 * Make LB** - 2Liters - and autoclave
 * Make 5 M NaCl** - 500 ml in water, sterile filter into a bottle, label, store at Room Temp


 * Make Kanamycin**- stocks 50ug/ul store in -20degC, Make 30 tubes of 500 ul each. __Sterile filter with syringe filter__before aliquoting into tubes. Label. Store in -20degC


 * Make IPTG** - 1 M IPTG stock to make. Make 30 tubes of this with 500 ul in each. Label. Store in -20degC


 * Make Lysozyme** - 20 aliquots of 500 ul at 50 mg/ml in 1.7 ml tubes. Label. Store in -20degC


 * Make TCEP -** make ~50 x 100ul aliquots of 500 mM aliquots medium size centrifuge tubes. Label, put in a box and Store at Room Temp in cubby.


 * Make PMSF -** 30 x 500 ul aliquots of 10mM in **__Isop____ropanol__** 1.7 ml tubes. Label. store in -20degC


 * Make Imidazole** - 1M stock **make in beaker of 500 ml of water, then bottle top sterile filter it into a bottle, store at Room Temp**

Lysis Buffer for Sonication (make 500 ml)
Tris 100 mM NaCl 300 mM Imidazole 30 mM pH 8.0 w/ NaOH label, store at 4degC
 * (see GDocs '**Solutions&Dilutions**' in /**Misc **folder for calculations)**

Wash Buffer Tris 100 mM NaCl 300 mM Imidazole 30 mM pH 8.0 w/ NaOH
 * NEW Wash and Elution Buffers and Storage Buffer(see GDocs '**Solutions&Dilutions**' in /**Misc **folder for calculations)**

Tris 100 mM NaCl 300 mM Imidazole 300 mM pH 8.0 w/ NaOH
 * Elution Buffer**

Tris 50 mM NaCl 150 mM pH 8.0 w/ NaOH Then filter sterilize Then degass**
 * FPLC column buffer - tris Also 'storage' buffer**

6X SDS Page Buffer from 5 Prime Protocol
30 ml of glycerol – add first so that you can see the volume (viscous) (In a separate tube) - 30 ml of Nanopure water --- 1.635 g Tris powder --- 3 g SDS poweder --- 0.3 g Bromophenol blue 2.3136 g DTT Mix contents of second tube into tube with glycerol Add some more Nanopure water to 50 ml total final volume

010914 - see revised Beginning of Semester Q's Ver2 Please fill out the VDSstaffBeginningofSemesterQ's https://docs.google.com/spreadsheet/viewform?formkey=dDNjTjlhdTlaM1BZWE9TNEVEdnlIeHc6MA#gid=0

= Printer Installation =


 * 1) Login as Mentor, Start Menu,
 * 2) Devices And printers
 * 3) Add New Printer
 * 4) Add "local" printer
 * 5) Create New Port --> standard TCP pathway
 * 6) Enter IP 128.82.195.186
 * 7) Printer Name Lexmark C543
 * 8) Add Printer (if can't find printer do Window Update)
 * For installation of a different Printer (such as Dell) change the IP address and Printer Name**

Printer Name: Lexmark C543 IP: 128.83.195.186 Printer Name: Dell IP: 129.116.152.49