Target+Sp15+-+Acetyl-CoA+carboxylase+(Clostridium+botulinum)

Format for Individual Target pages (copy this list to new Target page and then fill in for your target):
//Clostridium botulinum// is a gram-positive, rod-shaped bacteria responsible for botulism. The toxin caused by //C. botulinum// affects nerves and, if untreated, can cause paralysis and respiratory failure. Transmission is usually foodborne and can come from home-canned foods with low acid content or sealed containers. Enzyme is essential in fatty acid and phospholipid metabolism in //C. botulinum//. Essentiality was determined for //C. botulinum// and the homology between the selected protein and human orthologs was taken into account. The enzyme is a non-human homologous sequence. [] (paper determining the essentiality of different proteins in C. botulinum) Is it a monomer or multimer as biological unit ** ? (make prediction at ** @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html ): multimer Inhibition of acetyl-CoA carboxylase (ACC) by isozyme-specific antisense oligonucleotides or isozyme-nonselective ACC inhibitors. [] Inhibition of acetyl-CoA carboxylase is also inhibited by nanomolar concentrations of both haloxyfop and tralkoxydim. []
 * *Target (protein/gene name): **Acetyl-CoA carboxylase
 * *NCBI Gene # or RefSeq#: **441770
 * *Protein ID (NP or XP #) or Wolbachia#: **
 * *Organism (including strain): ** //Clostridium botulinum// (strain ATCC 19397/Type A)
 * Etiologic Risk Group (see link below): **
 * */ Disease Information (sort of like the Intro to your Mini __Research Write__ up): **
 * Link to TDR Targets page (if present): **
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) **[]
 * Essentiality of this protein: **
 * Complex of proteins?: **complex
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **

http://www.brenda-enzymes.info/enzyme.php?ecno=6.4.1.2
 * *EC#: 6.4.1.2 **
 * Link to BRENDA EC# page: **

Spectrophotometric assay coupling the carboxylation of acetyl-CoA to NADPH-dependent reduction of malonyl-CoA. []
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): **

PDB structure: []
 * Structure (PDB or Homology model) **

[] http://www.sciencedirect.com/science/article/pii/S1046592806001781 code MDFKGIENLIKAMSESNLSSMDIEYNGIAIKMKKENNKIYKQETISQEYEKENRDNIVEE KKLDLLSNEEKTGIAIADNLIEIVSPIVGTFYESPGVDKKPYAKAGDKVKKGDTVCIVEA MKVMNEIEAEVDGEIVEVLVENEQMVQYGEVLFKIKPL code 17.85449 kDa ATGGATTTTA AAGGCATTGA AAATTTGATC AAAGCGATGT CTGAGAGCAA CCTGTCCTCA ATGGATATTG AATACAATGG TATTGCGATT AAAATGAAAA AGGAAAACAA CAAAATTTAT AAACAAGAAA CTATCTCACA AGAGTATGAG AAAGAAAATC GGGATAATAT CGTAGAGGAA AAGAAACTGG ACCTGTTGTC AAACGAAGAG AAAACGGGTA TCGCGATTGC CGATAATCTT ATTGAGATTG TTAGCCCAAT CGTTGGTACG TTCTATGAGT CCCCGGGCGT GGACAAAAAA CCATATGCGA AAGCGGGCGA CAAAGTGAAA AAAGGGGATA CCGTGTGCAT CGTTGAAGCT ATGAAAGTAA TGAACGAGAT TGAAGCGGAA GTGGATGGGG AAATTGTCGA AGTCCTGGTG GAGAATGAAC AAATGGTTCA ATACGGTGAA GTTCTGTTTA AGATCAAACC CCTG
 * Current Inhibitors: **589
 * Expression Information (has it been expressed in bacterial cells): **has been expressed in E. Coli
 * Purification Method : **Myc-5 IgG affinity column
 * Image of protein (PyMol with features delineated and shown separately): **
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **
 * *length of your protein in Amino Acids: **158
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website: **
 * Molar Extinction coefficient of your protein at 280 nm wavelength: ** 8940
 * TMpred graph Image ** ( @http://www.ch.embnet.org/software/TMPRED_form.html ). Input your amino acid sequence to it.
 * *CDS Gene Sequence (paste as text only): **
 * [|gi 261417741] 4 MKIQEIRELIRLVDQSSIDEFVYEQGETKVHMKK .[16]. V APP .[9]. PAPAAPP .[4]. ASEA PAP KA 86 [|gi 146319455] 1 MNITEIKDLMSQFDQSSLREFSYSNAGEILHFSK .[12]. E APL .[9]. PVPAVEM SEPA PAE AG 75 [|gi 146321652] 1 MNITEIKDLMSQFDQSSLREFSYSNAGEILHFSK .[14]. P LAP .[7]. PVPAVEM SEPA PAE AG 75 [|gi 253752470] 1 MNITEIKDLMSQFDQSSLREFSYSNAGEILHFSK .[14]. P LAP .[7]. PVPAVEM SEPA PAE AG 75 [|gi 253754296] 1 MNITEIKDLMSQFDQSSLREFSYSNAGEILHFSK .[14]. P LAP .[7]. PVPAVEM SEPA PAE AG 75 [|gi 253756230] 1 MNITEIKDLMSQFDQSSLREFSYSNAGEILHFSK .[14]. P LAP .[7]. PVPAVEM SEPA PAE AG 75 [|gi 288904558] 2 VNISEVKDLMAQFDQSSLREFSFKTGEAELTFSK .[17]. A APV EVAATAP VAPA .[1]. AAS .[1]. SE 74 [|gi 55820480] 1 MNISEIKDLLAQFDTSTLREFSYKNNGEELNLSK .[22]. Q APV .[1]. PVAAPAA .[1]. ETPA .[1]. PVE EA 79 [|gi 55822365] 1 MNISEIKDLLAQFDASTLREFSYKNNGEELNLSK .[19]. S SPQ .[4]. PVAAPAA .[1]. ETPA .[1]. PVE EA 79 [|gi 116627300] 1 MNISEIKDLLAQFDASTLREFSYKNNGEELNLSK .[19]. S SPQ .[4]. PVAAPAA .[1]. ETPA .[1]. PVE EA 79 ||
 * [|gi 261417741] 87 ET .[11]. GNLHQITSPMVGTFYAAPAPDKPPYVKPGDKVKKDTVVCIIEAMKLFNEIEAEVDGEIVEVLVQNGQL 167 [|gi 146319455] 76 AS .[ 2]. AEGAVVESPLVGVAYLSPAPDKPAFVAVGDTVKKGQTLMIIEAMKVMNEVPADRDGVVTEILVANQML 147 [|gi 146321652] 76 AS .[ 2]. AEGAVVESPLVGVAYLSPAPDKPAFVAVGDTVKKGQTLMIIEAMKVMNEVPADRDGVVTEILVANQDV 147 [|gi 253752470] 76 AS .[ 2]. AEGAVVESPLVGVAYLSPAPDKPAFVAVGDTVKKGQTLMIIEAMKVMNEVPADRDGVVTEILVANQDV 147 [|gi 253754296] 76 AS .[ 2]. AEGAVVESPLVGVAYLSPAPDKPAFVAVGDTVKKGQTLMIIEAMKVMNEVPADRDGVVTEILVANQDV 147 [|gi 253756230] 76 AS .[ 2]. AEGAVVESPLVGVAYLSPAPDKPAFVAVGDTVKKGQTLMIIEAMKVMNEVPADRDGVVTEILVANQDV 147 [|gi 288904558] 75 ET .[ 7]. AEGDEVTSPLVGVAYLAPAPDKPAFVSVGDSVKKGQTLLIIEAMKVMNEIPAPNDGIVTEIMVNNEDV 151 [|gi 55820480] 80 SA .[ 2]. AEGEVVESPLVGVAYLSPSPEKPAFVSVGDTVKKGQTLLIVEAMKVMNEVPAPKDGVITEILVANEEV 151 [|gi 55822365] 80 SA .[ 2]. AEGEVVESPLVGVAYLSPSPEKPAFVSVGDTVKKGQTLLIVEAMKVMNEVPAPKDGVITEILVANEEV 151 [|gi 116627300] 80 SA .[ 2]. AEGEVVESPLVGVAYLSPSPEKPAFVSVGDTVKKGQTLLIVEAMKVMNEVPAPKDGVITEILVANEEV 151 ||
 * [|gi 261417741] 168 .[11]. 178 [|gi 146319455] [|gi 146321652] 148 .[11]. 158 [|gi 253752470] 148 .[11]. 158 [|gi 253754296] 148 .[11]. 158 [|gi 253756230] 148 .[11]. 158 [|gi 288904558] 152 .[11]. 162 [|gi 55820480] 152 .[11]. 162 [|gi 55822365] 152 .[11]. 162 [|gi 116627300] 152 .[11]. 162 ||
 * *GC% Content for gene: **6.45%
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **
 * *GC% Content for gene (codon optimized): **39.87%

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)

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 * Primer design results for 'tail' primers (this is just 2 sequences): **