ARIANA+L.

__ Title: __

__ Introduction: __

__ Materials & Methods: __

__ Results: __ **Figure 1a**: Fun plate containing no ampicillin swabbed with dirt from bottom of boot. Growth of bacteria developed after stored in 37 degree Celsius incubator over night. Creamy yellow dots represent individual bacteria colony, and droplets on plate represent condensation. 3 letter initials of each partner written, along with VDS, date, and “Fun Plate” that contained no DNA.

**Figure 1b**. Experimental Agar plate containing antibiotic Ampicillin, organism BL21 (DE3), and plasmid vector pGEM-gbr22. Growth of bacteria developed after stored in 37 degree Celsius incubator over night. Creamy yellow dots represent individual bacteria colony, and droplets on plate represent condensation. 3 letter initials of each partner written, along with VDS, date, organism BL21 (DE3), and plasmid vector pGEM-gbr22.

**Figure 1c.** Control agar plate containing organism BL21 (DE31), and antibiotic Ampicillin. No growth of bacteria developed after stored in 37 degree Celsius incubator over night due. Droplets on plate represent condensation. 3 letter initials of each partner written, along with VDS, date, organism BL21 (DE3), and “No DNA”.

**Figure 2.** Purple culture in each 125 ml Erlenmeyer flask. Covered with aluminum foil, and tape for sterile reasons when being placed in incubator at 37 degree Celsius for 16-24 hours. Cloudiness represents that bacteria has grown. Labeled with initials, media LB + AMP, organism: BL21 (DE3), and plasmid: pGEM-gbr22.



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**Figure 3.** Tubes containing 2.5 ml of 1x PBS with cell pellets in 50ml conical tube that were vortex to obtain even suspension of cells. Wet pellet weight of first tube was 0.11g. Wet pellet weight of second tube was 0.15 g. ======

**Figure 4**. 15 mL conical tubes containing gbr-22 after being eluded with the Elusion buffer. Elusion 1 is purple because a majority of the protein came out after the first round of Elution buffer was used, which then caused the second round to only have a little, if any, protein left.

**Figure 5.** Gel after staining with 6x loading die.The leftmost column is the protein band, followed by the cell lysate, soluble fraction, flow through, wash, elution 1, and elution 2.
 * Figure 6.** Gel wrapped in cellophane wrap, after pulled out of the drying bed. The leftmost column is the protein band, followed by the cell lysate, soluble fraction, flow through, wash, elution 1, and elution 2.

__ Discussion: __

__ Conclusions: __

__ References: __