MentorsSumm15

=**MENTOR TASKS**= NOTE: the best way to figure out what you need to work on is to do 'Ctrl+F' and search for your name. Hit 'enter' to find the next instance. However, some tasks say '**All**' so your name won't show up specificallly - Thanks, Dr. B - this assumes I haven't misspelled your name!

Lab Safety mentors - Charina, Andee

Please fill out your weekly schedule here:- **ALL**

Use the MentorLabLog on GDocs/VDSstaff for a Lab Notebook for Wet Lab tasks - **ALL**

Put new dates on Daily Schedule (i.e. take last year's items for each week and put the new dates for this year there) - **Andee** . Dr. B already did Week 0, Week 1, Week 2

Send out FRI Facebook link -**Andee** Send out FRI LinkeIn link - **whichever one of you has a LinkeIn profile**

Journal Club
Set up journal club page for Summer 2015 -**Nikki DONE** make schedule of presenters -**Nikki (in progress)**

Research Presentations Schedule - Nikki
- Mentors to update - but not yet (need some research first) - will be on the Journal Club page since that will also have the list of Journal Club presentations

Lab Safety (Monday)
LAB SAFETY MENTORS: **Andee, Charina** Update Lab Safety page in Wikispaces with names of each students and table, link from left hand menu. See Lab Safety Folder in GDrive/VDSstaff - Practice & Plan Lab Safety Walk Throughs. OH 201 Lab Safety Walk through – Charina and Nikki to lead (or Andee depending on timing)

Print Pink & Blue & Orange Sign Off Sheet for Site Specific training (Autoclave, Centrifuge) in GDocs/Misc/ --- SignOffSheet_SiteSpecific_Centrifuge_Autoclave_etc_Blue.docx - --- SignOffSheet_SiteSpecific_Centrifuge_Autoclave_etc_Pink.docx - --- SignOffSheet_SiteSpecific_Centrifuge_Autoclave_etc_Pink.docx - Show these to the students during safety training Afix these to the Big Centrifuge (in DIY lab), and the Sonicator (in our lab) Wait to Afix these to the FPLC (? not yet), Autoclave (? - not yet),
 * See One, Do One, Teach One**

- designate Summer Plasmids for students to Transform and make more of this summer - **Andee**
 * @Summer Plasmids**

Update Hours spreadsheet in GDocs - Andee Charina Tally weekly hours from sign in sheet and enter into spreadsheet on VDSStaff - Andee or Nikki
 * Hours for Students**

Create **Research Pages** for each student (OR SHOULD WE JUST DO DROPBOX FOLDERS?) - (use Research Pages 2014 as a guide)

- make Target Selection form (copy from Summ14 - or make fresh) - once we know which targets are options
 * Targets**

PCR, RE Digest, Cloning, etc. - Charina - Nikki - Andee
 * Teaching Modules** (in class brief activities – 5 slides, 1 activity, 2 slides)

Lab Tech Tasks (John G) - see Purchasing sheet on GDrive for items
@https://drive.google.com/open?id=19gXV3i-WeguSKluGbEH21akQqgE--S4UhNEUKmzalx4&authuser=0

Open, figure out if it is VDS, show to John G. if there, Label appropriately, Place in proper storage, Place receipt in Dr. B's pigeon hole by the lab notebooks and let him know it has arrived and is stored properly.
 * What do I do with a package that is delivered to lab?: **

Blue Juice for protein gels - **(need DTT - check first) -** Rinse the 'clean' collirollers in nanopure water, transfer to a clean bottle, label - then Autoclave (use autoclave tape) **- make students do** Divide up colirrolers in to 6 rollers each in sterile tubes of (600 ul tubes) - make a full ~ 80 tube rack of these - **make students do** Autoclave Nanopure water, label, store in Cubby. Make 2 x 500ml. - this is for 1st Lab to make 30% EtOH - **make students do**
 * LAB WORK**

**SOLUTIONS RECIPES & INSTRUCTIONS:**

 * (also see the Solutions&Dilutions Spreadsheet on GDocs for help with calculations)**


 * Make LB** - 2Liters - and autoclave
 * Make 5 M NaCl** - 500 ml in water, sterile filter into a bottle, label, store at Room Temp


 * Make Kanamycin**- stocks 50ug/ul store in -20degC, Make 30 tubes of 500 ul each. __Sterile filter with syringe filter__before aliquoting into tubes. Label. Store in -20degC


 * Make IPTG** - 1 M IPTG stock to make. Make 30 tubes of this with 500 ul in each. Label. Store in -20degC


 * Make Lysozyme** - 20 aliquots of 500 ul at 50 mg/ml in 1.7 ml tubes. Label. Store in -20degC


 * Make TCEP -** make ~50 x 100ul aliquots of 500 mM aliquots medium size centrifuge tubes. Label, put in a box and Store at Room Temp in cubby.


 * Make PMSF -** 30 x 500 ul aliquots of 10mM in **__Isop____ropanol__** 1.7 ml tubes. Label. store in -20degC


 * Make Imidazole** - 1M stock **make in beaker of 500 ml of water, then bottle top sterile filter it into a bottle, store at Room Temp**

Lysis Buffer for Sonication (make 500 ml)
Tris 100 mM NaCl 300 mM Imidazole 30 mM pH 8.0 w/ NaOH label, store at 4degC
 * (see GDocs '**Solutions&Dilutions**' in /**Misc **folder for calculations)**

Wash Buffer Tris 100 mM NaCl 300 mM Imidazole 30 mM pH 8.0 w/ NaOH
 * NEW Wash and Elution Buffers and Storage Buffer(see GDocs '**Solutions&Dilutions**' in /**Misc **folder for calculations)**

Tris 100 mM NaCl 300 mM Imidazole 300 mM pH 8.0 w/ NaOH
 * Elution Buffer**

Tris 50 mM NaCl 150 mM pH 8.0 w/ NaOH Then filter sterilize Then degass**
 * FPLC column buffer - tris Also 'storage' buffer**

6X SDS Page Buffer from 5 Prime Protocol
30 ml of glycerol – add first so that you can see the volume (viscous) (In a separate tube) - 30 ml of Nanopure water --- 1.635 g Tris powder --- 3 g SDS poweder --- 0.3 g Bromophenol blue 2.3136 g DTT Mix contents of second tube into tube with glycerol Add some more Nanopure water to 50 ml total final volume

= Printer Installation =
 * 1) Login as Mentor, Start Menu,
 * 2) Devices And printers
 * 3) Add New Printer
 * 4) Add "local" printer
 * 5) Create New Port --> standard TCP pathway
 * 6) Enter IP 128.82.195.186
 * 7) Printer Name Lexmark C543
 * 8) Add Printer (if can't find printer do Window Update)
 * For installation of a different Printer (such as Dell) change the IP address and Printer Name**

Printer Name: Lexmark C543 IP: 128.83.195.186 Printer Name: Dell IP: 129.116.152.49

=COMPLETED STUFF++++++++++++++++++++++++++++++++++++++++++++++++++++++++=

MentorsSp15 (last semester's mentors page)
M 3-4, T 4-5, F 2-3