Phosphatidylglycerophosphatase+B-


 * *Target (protein/gene name): ** Phosphatidylglycerophosphatase B
 * *NCBI Gene # or RefSeq#: ** 444351067
 * *Protein ID (NP or XP #) or Wolbachia#: ** YP_007387211.1
 * *Organism (including strain): ** Enterobacter aerogenes
 * Etiologic Risk Group (see link below): ** Escherichia coli - all enteropathogenic, enterotoxigenic, enteroinvasive and strains bearing K1 antigen, including E. coli O157:H7

Enterobacter aerogenes is a gram-negative, pathogenic bacteria that is commonly found in urinary and digestive tract infections. This bacterium is of special importance because of its ability to easily develop antibiotic resistance to common drugs. This has caused the pathogen to be frequently found in hospitals, where it has the ability to mutate to resist current antibiotics and other drugs. The bacterium does this by developing several pump-like mechanisms that push out harmful molecules from its cytosol. One of these pumps specifically uses a proton motive force to thrust out detergents disinfectants and antibiotics from the cell. It is because of these unique mechanisms the bacterium develops to become resistant to antibiotics that makes this a good target for virtual screening. If a specific essential protein, such as Phosphatidylglycerophosphatase B, can be identified and shut off in the organism then it can be degraded without the risk of having it develop antibiotic resistance. ( [])
 * *Background/Disease Information (sort of like the Intro to your Mini Research Write up): **


 * Essentiality of this protein: **In phospholipid synthesis/metabolism
 * Complex of proteins?: **No
 * Druggable Target: phosphatidic acid ( ** [|http://books.google.com/books?hl=en&lr=&id=sqp91vnUnaQC&oi=fnd&pg=PA215&dq=Phosphatidylglycerophosphatase+Enterobacter+aerogenes&ots=cdxKc1NYfu&sig=buVtgLc9DbB7qzMa3IY7HGGDTJE#v=onepage&q=Phosphatidylglycerophosphatase%20&f=false] )

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 * *EC#: ** 3.1.3.27
 * Link to BRENDA EC# page: **
 * -- ** Show screenshot of BRENDA enzyme mechanism schematic

J Bacteriol. 1977 October; 132(1): 159–165. PMCID: PMC221840 Intracellular Distribution of Enzymes of Phospholipid Metabolism in Several Gram-Negative Bacteria Anti-PGS1 (AB1) antibody produced in rabbit, Sigma, $335
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): ** decarboxylase
 * -- link to Sigma (or other company) page for assay or assay reagents (substrates) **
 * -- link (or citation) to paper that contains assay information **
 * -- List cost and quantity of substrate reagents and supplier **

-- PDB # or closest PDB entry if using homology model: **No structure found** -- For Homology Model option: Show pairwise alignment of your BLASTP search in NCBI against the PDB Query Coverage: Max % Identities: % Positives Chain used for homology:
 * Structure Available (PDB or Homology model) **


 * Current Inhibitors: **
 * Expression Information (has it been expressed in bacterial cells): ** No information was found about expression in other bacterial cells, but according to the paper referenced, the organism ( Enterobacter aerogenes) shares a 78% overall sequence identity in a 215-amino-acid (aa) overlap. This should mean that it would relatively easy to express the Phosphatidylglycerophosphatase B protein in a common bacteria such as //E. Coli//.

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Ion-exchange chromatography and Sephadex G-100 filtration ([|http://books.google.com/books?hl=en&lr=&id=sqp91vnUnaQC&oi=fnd&pg=PA215&dq=Phosphatidylglycerophosphatase+Enterobacter+aerogenes&ots=cdxKc1NYfu&sig=buVtgLc9DbB7qzMa3IY7HGGDTJE#v=snippet&q=purification&f=false])
 * Purification Method: **

//Protein shown in Mus musculus (could not be found in Enterobacter aerogenes) //
 * Image of protein (PyMol with features delineated and shown separately): **


 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **

1 mlliakrtai gaalllvmpv ivwlsgwqwq pgqapamlkm wywvtetvtq pwgiithval

61 fgwflwclrf rlrealvlfl ilgaailagq glkswikerv qeprpfviwl ertqqvpvaq

121 fyelkrkera klvheqlaeq qtipgflrkh wqketgfafp sghtmfaasw allgvgllwp

181 rrrwvtigll lgwatavmgs rlalgmhwpq dlivatslsw llvtaatwlt qrfcgpltpp

241 veeasdikkr at

Ext. coefficient 102105 Abs 0.1% (=1 g/l) 3.542, assuming all pairs of Cys residues form cystines Ext. coefficient 101980 Abs 0.1% (=1 g/l) 3.537, assuming all Cys residues are reduced gi|444350194:919247-920005 Enterobacter aerogenes EA1509E, complete genome
 * *length of your protein in Amino Acids **252 aa
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam]website: ** 28828.3
 * Molar Extinction coefficient of your protein at 280 nm wavelength: **
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it. [[image:http://www.ch.embnet.org/wwwtmp/.TMPRED.7199.8508.gif]]
 * *CDS Gene Sequence (paste as text only): **

ATGCTGTTGATTGCCAAAAGGACCGCCATCGGGGCGGCGCTATTGCTTGTTATGCCCGTTATAGTCTGGC

TCTCCGGCTGGCAGTGGCAGCCGGGTCAAGCACCGGCTATGTTGAAAATGTGGTACTGGGTGACTGAAAC

CGTCACCCAGCCGTGGGGAATAATTACTCACGTAGCGCTATTTGGCTGGTTTTTGTGGTGTTTACGATTT

CGTCTGCGCGAAGCGCTGGTGCTGTTTCTCATTCTCGGCGCCGCTATCCTGGCAGGACAGGGGCTTAAAT

CCTGGATCAAAGAACGGGTGCAGGAACCACGGCCGTTTGTGATTTGGTTAGAACGAACCCAACAGGTTCC

GGTGGCGCAATTCTATGAGTTAAAACGTAAAGAACGCGCTAAACTGGTGCATGAGCAATTGGCTGAACAA

CAGACAATACCGGGGTTCTTGCGTAAACACTGGCAAAAAGAGACCGGTTTTGCCTTTCCGTCCGGCCATA

CCATGTTTGCCGCCAGTTGGGCGCTACTGGGGGTAGGGCTGTTGTGGCCGCGCCGGCGCTGGGTAACTAT

CGGTTTGCTGTTGGGTTGGGCTACTGCGGTCATGGGCAGTCGTCTGGCGTTGGGCATGCACTGGCCGCAG

GATCTTATCGTGGCGACCAGCCTTTCATGGCTGCTGGTTACCGCAGCGACCTGGCTCACGCAGCGTTTTT

GCGGCCCGTTAACCCCGCCTGTTGAAGAGGCGAGCGATATCAAAAAACGCGCAACCTGA
 * *GC% Content for gene: ** 55.6%
 * *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): **
 * *GC% Content for gene (codon optimized): **