Viral+Neurominidase, Influenzavirus+A

Format for Individual Target pages (copy this list to new Target page and then fill in for your target):

 * Target (protein/gene name):** Viral neuraminidase
 * NCBI Gene # or RefSeq#:** NC_001422
 * Protein ID (NP or XP #) or Wolbachia#:**
 * Organism (including strain):** Orthomyxoviridae: Influenzavirus A
 * Etiologic Risk Group (see link below):**
 * / Disease Information (sort of like the Intro to your Mini Research Write up):**

The flu, also called Influenza, is an infectious disease which is instigated by the influenza virus. Influenza is most commonly experienced with a high fever, runny nose, sore throat, and feeling exhausted. Symptoms typically last than a week and are usually experienced two days after being exposed to the virus. There are three strains of the virus: Type A, Type B, and Type C and all are usually spread though the air. There is typically a global annual outbreak of Influenza, which results in a quarter million to half a million deaths. Viral neuraminidase is an enzyme located on the virus' surface and plays an essential role in the organism virulence. It allows the virus to be released from the host cell and pass on to infect other organisms. Viral neuraminidase work by removing the sialic acid group from a glycoprotein and are also essential for the rapid replication of the influenza virus.

https://www.ncbi.nlm.nih.gov/gene/2546398 Is it a monomer or multimer as biological unit**? (make prediction at** @http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): multimer Viral neuraminidase has been marked as a promising target but has yet been used to make an effective drug. Ulrike Grienke; Michaela Schmidtke; Grafenstein, S.; Johannes Kirchmair; R. Liedl, K.; M. Rollinger, J. Influenza neuraminidase: A druggable target for natural products http://pubs.rsc.org/en/content/articlehtml/2012/np/c1np00053e (accessed May 5, 2018)
 * Link to TDR Targets page (if present):** N/A
 * Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.)**
 * Essentiality of this protein:** required for host cell to infect other organisms
 * Complex of proteins?:** Yes
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):**


 * EC#:**
 * Link to BRENDA EC# page:**
 * --** Show screenshot of BRENDA enzyme mechanism schematic

-cell culture-based assays -in vitro assays with bacterial NA, like C. perfringens and V. cholerae, to pinpoint anti0viral agents using fluorescence and chemiiluminescence based testing systems that work with viral and bacterial NA
 * Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):**

 http://pubs.rsc.org/en/content/articlehtml/2012/np/c1np00053e https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/General_Information/2/biofiles_issue10.pdf N/A because unspecified volume, Sigma-Aldrich
 * -- link to Sigma (or other company ) page for assay (see Sigma links below)**
 * -- -or link (or citation) to paper that contains assay information**
 * -- links to assay reagents (substrates) pages.**
 * --- List cost and quantity of substrate reagents, supplier, and catalog #**

-- PDB # or closest PDB entry if using homology model: 7NN9 -- For Homology Model option: N/A Show pairwise alignment of your BLASTP search in NCBI against the PDB N/A Query Coverage: N/A Max % Identities: N/A % Positives N/A Chain used for homology: N/A
 * Structure (PDB or Homology model)**


 * Current Inhibitors:** zanamivir, oseltamivir, peramivir

code MNPNQKIITI GSVSLTIATV CFLMQIAILV TTVTLHFKQH DCDSPASNQV 050 MPCEPIIIER NITEIVYLNN TTIEKEICPK VVEYRNWSKP QCQITGFAPF 100 SKDNSIRLSA GGDIWVTREP YVSCDPVKCY QFALGQGTTL DNKHSNDTVH 150 DRIPHRTLLM NELGVPFHLG TRQVCIAWSS SSCHDGKAWL HVCITGDDKN 200 ATASFIYDGR LVDSIGSWSQ NILRTQESEC VCINGTCTVV MTDGSASGRA 250 DTRILFIEEG KIVHISPLAG SAQHVEECSC YPRYPGVRCI CRDNWKGSNR 300 PVVDINMEDY SIDSSYVCSG LVGDTPRNDD RSSNSNCRNP NNERGTQGVK 350 GWAFDNGNDL WMGRTISKDL RSGYETFKVI GGWSTPNSKS QINRQVIVDS 400 DNRSGYSGIF SVEGKSCINR CFYVELIRGR KQETRVWWTS NSIVVFCGTS 450 GTYGTGSWPD GANINFMPI                                   469 code
 * Expression Information (has it been expressed in bacterial cells):** yes
 * Purification Method :**
 * Image of protein (PyMol with features delineated and shown separately):**
 * Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):**

code format="genbank"                     MVRSYYPSECHADYFDFERIEALKPAIEACGISTLSQSPMLGFH KQMDNRIKLLEEILSFRMQGVEFDNGDMYVDGHKAASDVRDEFVSVTEKLMDELAQCY NVLPQLDINNTIDHRPEGDEKWFLENEKTVTQFCRKLAAERPLKDIRDEYNYPKKKGI KDECSRLLEASTMKSRRGFAIQRLMNAMRQAHADGWFIVFDTLTLADDRLEAFYDNPN ALRDYFRDIGRMVLAAEGRKANDSHADCYQYFCVPEYGTANGRLHFHAVHFMRTLPTG SVDPNFGRRVRNRRQLNSLQNTWPYGYSMPIAVRYTQDAFSRSGWLWPVDAKGEPLKA TSYMAVGFYVAKYVNKKSDMDLAAKGLGAKEWNNSLKTKLSLLPKKLFRIRMSRNFGM KMLTMTNLSTECLIQLTKLGYDATPFNQILKQNAKREMRLRLGKVTVADVLAAQPVTT NLLKFMRASIKMIGVSNLQSFIASMTQKLTLSDISDESKNYLDKAGITTACLRIKSKW TAGGK" code
 * length of your protein in Amino Acids** 469
 * Molecular Weight of your protein in kiloDaltons using the [|Expasy ProtParam] website** 45.55 kDa
 * Molar Extinction coefficient of your protein at 280 nm wavelength:** 12.6 l/mol*cm
 * TMpred graph Image** (@http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
 * CDS Gene Sequence (paste as text only):**
 * GC% Content for gene:** 61%
 * CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): N/A**
 * GC% Content for gene (codon optimized): N/A**

Do Not Need this info for Spring (but still copy these lines to your Target page for now) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):**
 * ( link to DNA Works output text file - **that should be saved in your Google Docs folder after you did the primer design protocol)


 * Primer design results for 'tail' primers (this is just 2 sequences):**