TargetSummer17+- protein+phosphatase+2B,+putative+(T.+brucei)


 * Target (protein/gene name): **
 * NCBI #: **
 * Protein ID (NP or XP #) or Wolbachia#: **
 * Organism (including strain): **
 * Etiologic Risk Group (see link below): **


 * Disease Information (sort of like the Intro to your Mini Research Write up): **
 * Link to TDR Targets page (if present): **
 * Link to Gene Database page NCBI: **

**Is it a monomer or multimer as biological unit:**
 * Essentiality of this protein: **


 * Complex of proteins?: **

A continuous, spectrophotometric assay is available. [|Link to paper.]
 * Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): **
 * *EC#: **
 * Link to BRENDA EC# page: **
 * Enzyme Assay information: **
 * -- links to assay reagents (substrates) pages. **
 * --- List cost and quantity of substrate reagents, supplier, and catalog # **

[|PDB] #: 2VPQ In eukaryotes, the BC, CT and BCCD subunits reside in a single polypeptide chain. In bacteria, ACC assembles as a multisubunit complex with each of the reactions performed by a separate enzyme. These differences in tertiary structure can provide for selective inhibitors.
 * Structure (PDB or Homology model) **
 * Similarity to Human: **

One weak inhibitor [|has been discovered]. Chain A Sequence: MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIADEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFIGPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATGGGGKGIRVARDEKELETGFRMTEQEA QTAFGNGGLYMEKFIENFRHIEIQIVGDSYGNVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFIYDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHAIEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESA CYTNYTIPPYYDSMVAKLIIHEPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDEG Chain B Sequence: MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIADEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFIGPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATAGGGGKGIRVARDEKELETGFRMTEQE AQTAFGNGGLYMEKFIENFRHIEIQIVGDSYGNVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFIYDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHAIEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIES ACYTNYTIPPYYDSMVAKLIIHEPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDEG ATGAAAAAGGTTTTAATTGCAAACCGCGGTGAAATCGCAGTTAGGATTATTCGCGCTTGTCGTGATTTAGGCATCCAAACTGTTGCAATCTATTCTGAAGGGGATAAAGATGCGCTACATACTCAAATTGCTGATGAAGCATATTGCGTAGGTCCCACTTTGTCTAAAGATTCATATTTAAATATTCCGAACATCTTATCT ATTGCAACTTCTACAGGTTGTGATGGCGTCATCCGGGTTATGGCTTTTTAGCTGAAAATGCTGACTTTGCAGAATTATGCGAAGCATGCCAATTGAAGTTCATTGACCAAGTTATCAATCTATCCAAAAAATGGGTATCAAAGATGTTGCTAAGGCAGAAATGATCAAAGCCAATGTTCCAGTTGTTCCTGGTAGTGAC GGTTTAATGAAAGACGTCTCAGAAGCTAAGAAAATCGCTAAGAAAATTGGCTATCCGGTCATCATTAAAGCTACTGCTGGTGGTGGCGGAAAAGGTATCCGTGTTGCTCGTGATGAAAAAGAACTTGAAACTGGCTTCCGAATGACAGAACAAGAAGCTCAAACTGCATTTGGTAATGGTGGACTTTATATGGAGA AATTCATCGAAAACTTCCGCCATATTGAAATCCAAATTGTTGGGGACAGCTATGGTAATGTAATTCATTTAGGAGAACGTGATTGTACAATTCAAAGACGTATGCAGAAATTAGTGGAAGAAGCACCTTCCCCAATTTTAGATGATGAAACACGTCGTGAAATGGGAAATGCCGCAGTTCGTGCAGCGAAAGCTGTA AATTATGAAAATGCGGGAACAATTGAGTTTATATATGATTTAAATGATAATAAATTTTATTTTATGGAAATGAATACACGTATTCAAGTAGAACATCCTGTAACTGAAATGGTAACAGGAATTGATTTAGTTAAATTACAATTACAAGTTGCTATGGGTGACGTGTTACCGTATAAACAAGAAGATATTAAATAACAGGACA CGCAATTGAATTTAGAATTAATGCTGAAAATCCTTACAAGAACTTTATGCCATCACCAGGTAAAATTGAGCAATATCTTGCACCAGGTGGATATGGTGTTCGAATAGAGTCAGCATGTTATACTAATTATACGATACCGCCATATTATGATTCGATGGTAGCGAAATTAATCATACATGAACCGACACGAGATGAAGCGAT TATGGCTGGCATTCGTGCATTAAGTGAATTTGTGGTTCTTGGTATTGATACAACTATTCCATTCCATATTAAATTATTGAATAACGATATATTTAGAAGCGGTAAATTTAATACAAACTTTTTAGAGCAAAATAGCATTATGAATGATGAAGGTTAA
 * Current Inhibitors: **
 * Expression Information (has it been expressed in bacterial cells): ** It can and has been expressed in // E. coli. // Expressed in cytoplasm. ACC is essential for growth, is expressed at all times, expression is also very regulated to maintain the correct stoichiometry of subunits.
 * Purification Method : ** Standard nickel affinity column chromatography can be used.
 * Image of protein (PyMol with features delineated and shown separately): **
 * *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): **
 * Length of your protein in Amino Acids: ** 451 amino acids.
 * Molecular Weight of your protein in kiloDaltons: ** 50.05 kDA
 * Molar Extinction coefficient of your protein at 280 nm wavelength: ** 28685 mol^-1cm-1
 * TMpred graph Image ** (@http://www.ch.embnet.org/software/TMPRED_form.html).
 * *CDS Gene Sequence (paste as text only): **

Do Not Need this info for Spring (but still copy these lines to your Target page for now)


 * *GC% Content for gene: **


 * output **


 * *GC% Content for gene (codon optimized): **

-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
 * Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): **
 * ( link to DNA Works output text file - ** that should be saved in your Google Docs folder after you did the primer design protocol)

**
 * Primer design results for 'tail' primers (this is just 2 sequences): **